CDw75 (ST6GAL1) (NM_173216) Human Untagged Clone

CAT#: SC127214

ST6GAL1 (untagged)-Human ST6 beta-galactosamide alpha-2,6-sialyltranferase 1 (ST6GAL1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDw75
• Synonyms: SIAT1; ST6GalI; ST6N
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127214 sequence for NM_173216 edited (data generated by NextGen Sequencing)
  ATGATTCACACCAACCTGAAGAAAAAGTTCAGCTGCTGCGTCCTGGTCTTTCTTCTGTTT
  GCAGTCATCTGTGTGTGGAAGGAAAAGAAGAAAGGGAGTTACTATGATTCCTTTAAATTG
  CAAACCAAGGAATTCCAGGTGTTAAAGAGTCTGGGGAAATTGGCCATGGGGTCTGATTCC
  CAGTCTGTATCCTCAAGCAGCACCCAGGACCCCCACAGGGGCCGCCAGACCCTCGGCAGT
  CTCAGAGGCCTAGCCAAGGCCAAACCAGAGGCCTCCTTCCAGGTGTGGAACAAGGACAGC
  TCTTCCAAAAACCTTATCCCTAGGCTGCAAAAGATCTGGAAGAATTACCTAAGCATGAAC
  AAGTACAAAGTGTCCTACAAGGGGCCAGGACCAGGCATCAAGTTCAGTGCAGAGGCCCTG
  CGCTGCCACCTCCGGGACCATGTGAATGTATCCATGGTAGAGGTCACAGATTTTCCCTTC
  AATACCTCTGAATGGGAGGGTTATCTGCCCAAGGAGAGCATTAGGACCAAGGCTGGGCCT
  TGGGGCAGGTGTGCTGTTGTGTCGTCAGCGGGATCTCTGAAGTCCTCCCAACTAGGCAGA
  GAAATCGATGATCATGACGCAGTCCTGAGGTTTAATGGGGCACCCACAGCCAACTTCCAA
  CAAGATGTGGGCACAAAAACTACCATTCGCCTGATGAACTCTCAGTTGGTTACCACAGAG
  AAGCGCTTCCTCAAAGACAGTTTGTACAATGAAGGAATCCTAATTGTATGGGACCCATCT
  GTATACCACTCAGATATCCCAAAGTGGTACCAGAATCCGGATTATAATTTCTTTAACAAC
  TACAAGACTTATCGTAAGCTGCACCCCAATCAGCCCTTTTACATCCTCAAGCCCCAGATG
  CCTTGGGAGCTATGGGACATTCTTCAAGAAATCTCCCCAGAAGAGATTCAGCCAAACCCC
  CCATCCTCTGGGATGCTTGGTATCATCATCATGATGACGCTGTGTGACCAGGTGGATATT
  TATGAGTTCCTCCCATCCAAGCGCAAGACTGACGTGTGCTACTACTACCAGAAGTTCTTC
  GATAGTGCCTGCACGATGGGTGCCTACCACCCGCTGCTCTATGAGAAGAATTTGGTGAAG
  CATCTCAACCAGGGCACAGATGAGGACATCTACCTGCTTGGAAAAGCCACACTGCCTGGC
  TTCCGGACCATTCACTGCTAA
  
  Clone variation with respect to NM_173216.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_173216 unedited
  TAACCCCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGC
  AGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCG
  AATTCGGCACGAGGACCGACCGGCGTGGGCGCGGAGCGGGCGGCCGCCACCGAGCGTGCT
  GAGCAACCGCAGCCTCCGCGGCCGAGAGTGCAGCGAGCAAGGGGAGAGCCAGTTGCGCAG
  AGCCCTGCAACCAGCAGTCCAGGGAGAAGTGGTGAATGTCATGGAGCCCAGCTGAAATGG
  ACTGGCCCCCTTGAGCCTGTCCCAAGCCCTGGTGCCAGGTGTCCATCCCCGTGCTGAGAT
  GAGTTTTGATCATCCTGAGAAAAATGGGCCTTGGCCTGCAGACCCAATAAACCTTCCCTC
  CCATGGATAATAGTGCTAATTCCTGAGGACCTGAAGGGCCTGCCGCCCCTGNGGGATTAG
  CCAGAAGCAGGCTTGTTTTCCTGCTCAGAACAAAGTGACTTCCCTGAACACATCTTCATT
  ATGATTCACACCAACCTGAAGAAAAAGTTCAGCTGCTGCGTCCTGGTCTTTCTTCTGTTT
  GCAGTCATCTGTGTGTGGAAGGAAAAGAAGAAAGGGAGTTACTATGATTCCTTTACATTG
  CAAACCAAGGAATTCCAGGTGTTAAAGAGTCTGGNGACATTGGCCATGGNGTCTGATTCC
  CAGTCTGTATNCTCAAGCAGCACCCAGAACCCCCACAGGGGCCGNCAGACCCTTCGCAGT
  CTCAGAGGCCTAGCCAAGCCAAACCAGAGGCCCTCTTCCAGGNTGTGGAACAGGNACAGC
  TCTTCCAAAACCTTATCCTAGCTGCAAAGATACTGGAGAATACCTAGCATGACCAGTCCA
  AGTGTCTACAAGGGCCAGACAGGCTCNAGTCAGTGCANAGCCCTGGCTGCACTCGACAAT
  GN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_173216 unedited
  CATCCAGTTGACCTATTGGGNGATGGTCAACTTCCCAGGNCCAGNAAAGCACTGGAGGAG
  GGTCACAGGGATGCCACCCGGGTTCTGTTCAGGAAAACAGCTATGACCGCGGCCGCAATC
  TAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAGAAACACACACACT
  TTTATTTTAAAATTTGACATAATCCTTGGGTAATTATTAGCATTTTTCAAAAGTTTCCGA
  GACTGATATCTCAACTCCAAACCAATAGCAATAGCTTATTTGATTGGTGGAAGGATTAGA
  GAGGGTCCCATGATCCAGATGAATAGATGGCTATCAAAGAAGACAGAACCCCATAGACAC
  AGGCAGCATTAACTCATAATTTTTAAATTAACTGTCACAAATATGAAACATCTCACCAGA
  CCAATGATAACGTCCATATCATAATTTGCATTTTACCAAAGTGTCCACATAGACAGCACA
  GAACTAAAAGTTCTGTAGACTTCAGAATCATATTTAAATTAATTTGATCCTTTATATTAA
  TAATAATTAAAAACTAGATTCTACCCAGTGTCGTCCCAGTCTTCACACCAACATGTATGT
  CCTGCTGTCGGAATGCCACCTCCTCTGCACTCAGTGTCATGATTCGAAGATTAAAATCAG
  TGTGTCAAATCAGAACATAAACCAAGCCAGGAATTCATGGGCCCAGAGAGGGGTAAGGGG
  CTTGTCCTATACAGCAGTTAAGCAGCCCACGTGCTTCGCCCATTTTCCAAGCAAAATCAN
  ATTGGAGCTGCAGGCAGCGAGGAAGAAGGCGTGTTCTGTGTTGNCCACTGCTTTGCATGG
  TTTCTTGGGTGTTCCCAAGGAGACAGTTTGTGTCTCCCAATAGACGTTAATTCCCTGAGG
  ACGGACC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_173216
• Insert Size: 4700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173216.1, NP_775323.1
• RefSeq Size: 4403 bp
• RefSeq ORF: 1221 bp
• Locus ID: 6480
• UniProt ID: P15907
• Cytogenetics: 3q27.3
• Protein Families: Secreted Protein
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• Gene Summary: This gene encodes a member of glycosyltransferase family 29. The encoded protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The protein, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CD75, and CD76. This gene has been incorrectly referred to as CD75. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2017]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1, 2 and 4 encode the same protein (isoform a).