FLIP (CFLAR) (NM_003879) Human Untagged Clone

CAT#: SC127121

CFLAR (untagged)-Human CASP8 and FADD-like apoptosis regulator (CFLAR), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FLIP
• Synonyms: c-FLIP; c-FLIPL; c-FLIPR; c-FLIPS; CASH; CASP8AP1; Casper; cFLIP; CLARP; FLAME; FLAME-1; FLAME1; FLIP; I-FLICE; MRIT
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127121 sequence for NM_003879 edited (data generated by NextGen Sequencing)
  ATGTCTGCTGAAGTCATCCATCAGGTTGAAGAAGCACTTGATACAGATGAGAAGGAGATG
  CTGCTCTTTTTGTGCCGGGATGTTGCTATAGATGTGGTTCCACCTAATGTCAGGGACCTT
  CTGGATATTTTACGGGAAAGAGGTAAGCTGTCTGTCGGGGACTTGGCTGAACTGCTCTAC
  AGAGTGAGGCGATTTGACCTGCTCAAACGTATCTTGAAGATGGACAGAAAAGCTGTGGAG
  ACCCACCTGCTCAGGAACCCTCACCTTGTTTCGGACTATAGAGTGCTGATGGCAGAGATT
  GGTGAGGATTTGGATAAATCTGATGTGTCCTCATTAATTTTCCTCATGAAGGATTACATG
  GGCCGAGGCAAGATAAGCAAGGAGAAGAGTTTCTTGGACCTTGTGGTTGAGTTGGAGAAA
  CTAAATCTGGTTGCCCCAGATCAACTGGATTTATTAGAAAAATGCCTAAAGAACATCCAC
  AGAATAGACCTGAAGACAAAAATCCAGAAGTACAAGCAGTCTGTTCAAGGAGCAGGGACA
  AGTTACAGGAATGTTCTCCAAGCAGCAATCCAAAAGAGTCTCAAGGATCCTTCAAATAAC
  TTCAGGCTCCATAATGGGAGAAGTAAAGAACAAAGACTTAAGGAACAGCTTGGCGCTCAA
  CAAGAACCAGTGAAGAAATCCATTCAGGAATCAGAAGCTTTTTTGCCTCAGAGCATACCT
  GAAGAGAGATACAAGATGAAGAGCAAGCCCCTAGGAATCTGCCTGATAATCGATTGCATT
  GGCAATGAGACAGAGCTTCTTCGAGACACCTTCACTTCCCTGGGCTATGAAGTCCAGAAA
  TTCTTGCATCTCAGTATGCATGGTATATCCCAGATTCTTGGCCAATTTGCCTGTATGCCC
  GAGCACCGAGACTACGACAGCTTTGTGTGTGTCCTGGTGAGCCGAGGAGGCTCCCAGAGT
  GTGTATGGTGTGGATCAGACTCACTCAGGGCTCCCCCTGCATCACATCAGGAGGATGTTC
  ATGGGAGATTCATGCCCTTATCTAGCAGGGAAGCCAAAGATGTTTTTTATTCAGAACTAT
  GTGGTGTCAGAGGGCCAGCTGGAGGACAGCAGCCTCTTGGAGGTGGATGGGCCAGCGATG
  AAGAATGTGGAATTCAAGGCTCAGAAGCGAGGGCTGTGCACAGTTCACCGAGAAGCTGAC
  TTCTTCTGGAGCCTGTGTACTGCGGACATGTCCCTGCTGGAGCAGTCTCACAGCTCACCA
  TCCCTGTACCTGCAGTGCCTCTCCCAGAAACTGAGACAAGAAAGAAAACGCCCACTCCTG
  GATCTTCACATTGAACTCAATGGCTACATGTATGATTGGAACAGCAGAGTTTCTGCCAAG
  GAGAAATATTATGTCTGGCTGCAGCACACTCTGAGAAAGAAACTTATCCTCTCCTACACA
  TAA
  
  Clone variation with respect to NM_003879.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003879 unedited
  GGGCACGTACACTATATTTGTAATACGACTCACTATAGGGCGGCCGCGCATAGCAGAAAC
  AGCGAGCTTGCAGCCTCACCGACGAGTCTCACTAAAAGGACTCCCGGAGCTAGGGGTGGG
  GACTCGGCCTCACACAGTGAGTGCCGGCTATTGGACTTTTGTCCAGTGACAGCTGAGACA
  ACAAGGACCACGGGAGGAGGTGTAGGAGAGAAGCGCCGCGAACAGCGATCGCCCAGCACC
  AAGTCCGCTTCCAGGCTTTCGGTTTCTTTGCCTCCATCTTGGGTGCGCCTTCCCGGCGTC
  TAGGGGAGCGAAGGCTGAGGTGGCAGCGGCAGGAGAGTCCGGCCGCGACAGGACGAACTC
  CCCCACTGGAAAGGATTCTGAAAGAAATGAAGTCAGCCCTCAGAAATGAAGTTGACTGCC
  TGCTGGCTTTCTGTTGACTGGCCCGGAGCTGTACTGCAAGACCCTTGTGAGCTTCCCTAG
  TCTAAGAGTAGGATGTCTGCTGAAGTCATCCATCAGGTTGAAGAAGCACTTGATACAGAT
  GAGAAGGAGATGCTGCTCTTTTTGTGCCGGGATGTTGCTATAGATGTGGTTCCACCTAAT
  GTCAGGGACCTTCTGGGATATTTACGGGGAAAGAGGTAAGCTGTCTGTCGGGGACTTGGC
  TGAACTGCTCTACAGAGTGAGGCGAATTGGCCTGCTCAAACGTATCTTGGAACATGGACA
  GAAAGCTGTGGAGACCCACCTTGCTCGGAACCCTCACCTTGGTTTCGGACTATTAAATGC
  TGGATGGCAAGATTGGTGAGGGATTTGGATAAACCTGAGGTGTCCCCATAAATTTTCCTC
  ATGAAGGATTCAATGGGCCGGGGCAAGATAACCAGGAAAAAAATT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003879 unedited
  NGGTATTACTATGNACGCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTAAAG
  AGAGGGATCTTGCTATATTGCCCAGGTTGGTATGCAGTGGCTATTCGTAGGCACAATCAC
  AGCATGCTATGACCCTGAACTCCTGGGTTCAAAAGATCCTCCCACCTCAGCCTCCCAAGT
  AACTGGGAATACAGGTACCCACACCCACACCCAGCTAATTTTTGTATTTTTACTAGGGAC
  AGCGTTTACCATGTTGGCCAGGCTGGTCTCGAACTCCTGACCTGAAGTGATCTGCCCTCC
  TTGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACTACGCCCAGCCTTTTGGTTTC
  TTATGTGTAGGAGAGGATAAGTTTCTTTCTCAGAGTGTGCTGCAGCCAGACATAATATTT
  CTCCTTGGCAGAAACTCTGCTGTTCCAATCATACATGTAGCCATTGAGTTCAATGTGAAG
  ATCCAGGAGTGGGCGTTTTCTTTCTTGTCTCAGTTTCTGGGAGAGGCACTGCAGGTACAG
  GGATGGTGAGCTGTGAGACTGCTCCAGCAGGGACATGTCCGCAGTACACAGGCTCCAGAA
  GAAGTCAGCTTCTCGGTGAACTGTGCACAGCCCCTCGCTTCTGAGCCTTGAATTCCACAT
  TTCTTCATCGCTGATCGCATCCACCTTCAAGAGGCTGCTGTCCTCCAGCTGGGCCTCTGA
  CACCACATAGTTCTGGATAAAAACATCTTTGGCTTCCCTGCTTGAAAAGGGCATGAATTC
  TCCCTGAACATCCTCCTGAAGTGATGCAGGGGGAGCCCTTGATGAGTCTGGTCCCACCAT
  ACACACTCTGGGGAGCCTCTTTGGGCTACCAGGACACCACCAAAGCTGCGGTAATACTCG
  GTGCTCGGGCATACCGGCAAATTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003879
• Insert Size: 2500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003879.3, NP_003870.3
• RefSeq Size: 2243 bp
• RefSeq ORF: 1443 bp
• Locus ID: 8837
• UniProt ID: O15519
• Cytogenetics: 2q33.1
• Domains: DED, CASc, ICE_p20
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Apoptosis
• Gene Summary: The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011]
  Transcript Variant: This variant (1) represents use of an alternate first exon and encodes the longest protein isoform (1). Variants 1 and 2 both encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.