NR1D1 (NM_021724) Human Untagged Clone

CAT#: SC127002

NR1D1 (untagged)-Human nuclear receptor subfamily 1, group D, member 1 (NR1D1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NR1D1
• Synonyms: ear-1; EAR1; hRev; REVERBA; REVERBalpha; THRA1; THRAL
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127002 sequence for NM_021724 edited (data generated by NextGen Sequencing)
  ATGACGACCCTGGACTCCAACAACAACACAGGTGGCGTCATCACCTACATTGGCTCCAGT
  GGCTCCTCCCCAAGCCGCACCAGCCCTGAATCCCTCTATAGTGACAACTCCAATGGCAGC
  TTCCAGTCCCTGACCCAAGGCTGTCCCACCTACTTCCCACCATCCCCCACTGGCTCCCTC
  ACCCAAGACCCGGCTCGCTCCTTTGGGAGCATTCCACCCAGCCTGAGTGATGACGGCTCC
  CCTTCTTCCTCATCTTCCTCGTCGTCATCCTCCTCCTCCTTCTATAATGGGAGCCCCCCT
  GGGAGTCTACAAGTGGCCATGGAGGACAGCAGCCGAGTGTCCCCCAGCAAGAGCACCAGC
  AACATCACCAAGCTGAATGGCATGGTGTTACTGTGTAAAGTGTGTGGGGACGTTGCCTCG
  GGCTTCCACTACGGTGTGCACGCCTGCGAGGGCTGCAAGGGCTTTTTCCGTCGGAGCATC
  CAGCAGAACATCCAGTACAAAAGGTGTCTGAAGAATGAGAATTGCTCCATCGTCCGCATC
  AATCGCAACCGCTGCCAGCAATGTCGCTTCAAGAAGTGTCTCTCTGTGGGCATGTCTCGA
  GACGCTGTGCGTTTTGGGCGCATCCCCAAACGAGAGAAGCAGCGGATGCTTGCTGAGATG
  CAGAGTGCCATGAACCTGGCCAACAACCAGTTGAGCAGCCAGTGCCCGCTGGAGACTTCA
  CCCACCCAGCACCCCACCCCAGGCCCCATGGGCCCCTCGCCACCCCCTGCTCCGGTCCCC
  TCACCCCTGGTGGGCTTCTCCCAGTTTCCACAACAGCTGACGCCTCCCAGATCCCCAAGC
  CCTGAGCCCACAGTGGAGGATGTGATATCCCAGGTGGCCCGGGCCCATCGAGAGATCTTC
  ACCTACGCCCATGACAAGCTGGGCAGCTCACCTGGCAACTTCAATGCCAACCATGCATCA
  GGTAGCCCTCCAGCCACCACCCCACATCGCTGGGAAAATCAGGGCTGCCCACCTGCCCCC
  AATGACAACAACACCTTGGCTGCCCAGCGTCATAACGAGGCCCTAAATGGTCTGCGCCAG
  GCTCCCTCCTCCTACCCTCCCACCTGGCCTCCTGGCCCTGCACACCACAGCTGCCACCAG
  TCCAACAGCAACGGGCACCGTCTATGCCCCACCCACGTGTATGCAGCCCCAGAAGGCAAG
  GCACCTGCCAACAGTCCCCGGCAGGGCAACTCAAAGAATGTTCTGCTGGCATGTCCTATG
  AACATGTACCCGCATGGACGCAGTGGGCGAACGGTGCAGGAGATCTGGGAGGATTTCTCC
  ATGAGCTTCACGCCCGCTGTGCGGGAGGTGGTAGAGTTTGCCAAACACATCCCGGGCTTC
  CGTGACCTTTCTCAGCATGACCAAGTCACCCTGCTTAAGGCTGGCACCTTTGAGGTGCTG
  ATGGTGCGCTTTGCTTCGTTGTTCAACGTGAAGGACCAGACAGTGATGTTCCTAAGCCGC
  ACCACCTACAGCCTGCAGGAGCTTGGTGCCATGGGCATGGGAGACCTGCTCAGTGCCATG
  TTCGACTTCAGCGAGAAGCTCAACTCCCTGGCGCTTACCGAGGAGGAGCTGGGCCTCTTC
  ACCGCGGTGGTGCTTGTCTCTGCAGACCGCTCGGGCATGGAGAATTCCGCTTCGGTGGAG
  CAGCTCCAGGAGACGCTGCTGCGGGCTCTTCGGGCTCTGGTGCTGAAGAACCGGCCCTTG
  GAGACTTCCCGCTTCACCAAGCTGCTGCTCAAGCTGCCGGACCTGCGGACCCTGAACAAC
  ATGCATTCCGAGAAGCTGCTGTCCTTCCGGGTGGACGCCCAGTGA
  
  Clone variation with respect to NM_021724.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021724 unedited
  TTTTGAATCCGACTTTCTATAGGGCGGCCGCGAATTCGGGCACGAGGGAGGCGCTCCCTG
  GGATCACATGGTACCTGCTCCAGTGCCGCGTGCGGCCCGGGAACCCTGGGCTGCTGGCGC
  CTGCGCAGAGCCCTCTGTCCCAGGGAAAGGCTCGGGCAAAAGGCGGCTGAGATTGGCAGA
  GTGAAATATTACTGCCGAGGGAACGTAGCAGGGCACACGTCTCGCCTCTTTGCGACTCGG
  TGCCCCGTTTCTCCCCATCACCTACTTACTTCCTGGTTGCAACCTCTCTTCCTCTGGGAC
  TTTTGCACCGGGAGCTCCAGATTCGCTACCCCGCAGCGCTGCGGAGCCGGCAGGCAGAGG
  CACCCCGTACACTGCAGAGACCCGACCCTCCTTGCTACCTTCTAGCCAGAACTACTGCAG
  GCTGATTCCCCCTACACACTCTCTCTGCTCTTCCCATGCAAAGCAGAACTCCGTTGCCTC
  AACGTCCAACCCTTCTGCAGGGCTGCAGTCCGGCCACCCCAAGACCTTGCTGCAGGGTGC
  TTCGGATCCTGATCGTGAGTCGCGGGGTCCACTCCCCGCCCTTAGCCAGTGCCCAGGGGG
  CAACAGCGGCGATCGCAACCTCTAGTTTGAGTCAAGGTCCAGTTTGAATGACCGCTCTCA
  GCTGGTGAAGACATGACGACCCTGGACTCCAACAACAACACAGGTGGCGTCATCACCTAC
  ATTGGCTCCAGTGGCTCCTCCCCAGCCGCACCAGCCCTGAATCCCTCTATAGTGACAACT
  CCAATGGCAGCTTCCAGTCCCTGACCCAGGCTGTCCCACTACTTCCACCATCCCCACTGG
  CTCCTCACCCAGACCCGCTCGTCCTTGGGGACATCCCCCACCTGATGAGACGCTCCCTCT
  T
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021724 unedited
  CCCCGCTATTCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCAGCTGTGAACTATTGGATTT
  GAGACAGGAACAGAACACCTCAGAGGGCCAGGGGAGGGTTGTGGGGGAGACATATTGGTT
  TAAATAGGGGAGGAGGGGAAGTTCGGTGATGGGGGAGGCAGGCTCCCCCCCCCCCTCCCC
  CTCCTTCTTTTTCCCCCCTCTCCCCCCCCCCCTTTTCCTCCCTTTCCCTCTTATCTTCTC
  CTCCCTCCTCCTCCCCTCCTCTCCTCCCCCCTCCCCCCCCCCCCCTCTCCTCCTCCCCCT
  CTTCCTTTCTCTCCTCTTTCCCCTCTTTCTCCCCTCTCCCTCTCCTCCCCCCCCCCCCGT
  TTTTTTCCTTTTTCTTTTCTTTTTTTTTCTTCCTCTTTCTCCTCCCTCCCCTTCTCCCCT
  CCTCCTTCTTTCCCTCCCTCCCCCCTCCCCCCCCTTTCTCTTCCCCTCTTCTTTCCCCTC
  TCCCCCACTCTCCCCCTCTCCCCCCTCTTTCCCTCCCTTACCTCCCTCCTCCCCCCTCCT
  CTCCCCTTCCTCTCCCTTTCCCCCCTTCCTCCCCCTCTCTCCCCTCCCTCCCTCTCCCCT
  CCCCTCCCCCCCCTTCTCCTCTCTCCCTTCCCCCCCCCCCTTCCCTTTTTTTCCCCCTCC
  TCTCCTCCCTCTCTTTTTTCTCCCCCCTCTCCCTTCCTCCCTACTCCTTTTTCTTTCTTT
  TCCCCTCCTCTCCCCCTCTCTCCTCCCTTCTTCCCTCCCCCCCTCCCCTCNTTTCTTCCT
  CTCTCTCCCTCCCCTCTCCCTCCCTCTCCTTCTCCTCCCTCTCCCTCTTCCCCTTCTTCT
  TTGTCCATCCTTCCATCACTTTCTCCCTGACTTCCTTTCTTCCTCATTCCTCTCTCGCCT
  CTTCCTTCTACCCCCCACTCCTCTCGCTTTTCCGTTTCCTCTCTCTTCTCTCGTCTTGCT
  CCCCCTCCTCCCCCTTCTCTTTCTCTCTCTCCCTCTCCTCTCTCTCCTTCCCTCTCCCCT
  CTACTCTCCGTCCTTCCTCCTCTTCTTTTCTCCTCCTTTATTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021724
• Insert Size: 2770 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021724.1, NP_068370.1
• RefSeq Size: 2768 bp
• RefSeq ORF: 1845 bp
• Locus ID: 9572
• UniProt ID: P20393
• Cytogenetics: 17q21.1
• Domains: HOLI, zf-C4
• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• Protein Pathways: Circadian rhythm - mammal
• Gene Summary: This gene encodes a transcription factor that is a member of the nuclear receptor subfamily 1. The encoded protein is a ligand-sensitive transcription factor that negatively regulates the expression of core clock proteins. In particular this protein represses the circadian clock transcription factor aryl hydrocarbon receptor nuclear translocator-like protein 1 (ARNTL). This protein may also be involved in regulating genes that function in metabolic, inflammatory and cardiovascular processes. [provided by RefSeq, Jan 2013]