EFEMP1 (NM_004105) Human Untagged Clone

SKU SC126895

EFEMP1 (untagged)-Human EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: EFEMP1
• Synonyms: DHRD; DRAD; EGF-containing fibulin-like extracellular matrix protein 1; FBLN3; FBNL; fibrillin-like; fibulin 3; FLJ35535; MGC111353; MLVT; MTLV; S1-5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126895 sequence for NM_004105 edited (data generated by NextGen Sequencing); ATGTTGAAAGCCCTTTTCCTAACTATGCTGACTCTGGCGCTGGTCAAGTCACAGGACACC; GAAGAAACCATCACGTACACGCAATGCACTGACGGATATGAGTGGGATCCTGTGAGACAG; CAATGCAAAGATATTGATGAATGTGACATTGTCCCAGACGCTTGTAAAGGTGGAATGAAG; TGTGTCAACCACTATGGAGGATACCTCTGCCTTCCGAAAACAGCCCAGATTATTGTCAAT; AATGAACAGCCTCAGCAGGAAACACAACCAGCAGAAGGAACCTCAGGGGCAACCACCGGG; GTTGTAGCTGCCAGCAGCATGGCAACCAGTGGAGTGTTGCCCGGGGGTGGTTTTGTGGCC; AGTGCTGCTGCAGTCGCAGGCCCTGAAATGCAGACTGGCCGAAATAACTTTGTCATCCGG; CGGAACCCAGCTGACCCTCAGCGCATTCCCTCCAACCCTTCCCACCGTATCCAGTGTGCA; GCAGGCTACGAGCAAAGTGAACACAACGTGTGCCAAGACATAGACGAGTGCACTGCAGGG; ACGCACAACTGTAGAGCAGACCAAGTGTGCATCAATTTACGGGGATCCTTTGCATGTCAG; TGCCCTCCTGGATATCAGAAGCGAGGGGAGCAGTGCGTAGACATAGATGAATGTACCATC; TCTCCATATTGCCACCAAAGATGCGTGAATACACCAGGCTCATTTTATTGCCAGTGCAGT; CCTGGGTTTCAATTGGCAGCAAACAACTATACCTGCGTAGATATAAATGAATGTGATGCC; AGCAATCAATGTGCTCAGCAGTGCTACAACATTCTTGGTTCATTCATCTGTCAGTGCAAT; CAAGGATATGAGCTAAGCAGTGACAGGCTCAACTGTGAAGACATTGATGAATGCAGAACC; TCAAGCTACCTGTGTCAATATCAATGTGTCAATGAACCTGGGAAATTCTCATGTATGTGC; CCCCAGGGATACCAAGTGGTGAGAAGTAGAACATGTCAAGATATAAATGAGTGTGAGACC; ACAAATGAATGCCGGGAGGATGAAATGTGTTGGAATTATCATGGCGGCTTCCGTTGTTAT; CCACGAAATCCTTGTCAAGATCCCTACATTCTAACACCAGAGAACCGATGTGTTTGCCCA; GTCTCAAATGCCATGTGCCGAGAACTGCCCCAGTCAATAGTCTACAAATACATGAGCATC; CGATCTGATAGGTCTGTGCCATCAGACATCTTCCAGATACAGGCCACAACTATTTATGCC; AACACCATCAATACTTTTCGGATTAAATCTGGAAATGAAAATGGAGAGTTCTACCTACGA; CAAACAAGTCCTGTAAGTGCAATGCTTGTGCTCGTGAAGTCATTATCAGGACCAAGAGAA; CATATCGTGGACCTGGAGATGCTGACAGTCAGCAGTATAGGGACCTTCCGCACAAGCTCT; GTGTTAAGATTGACAATAATAGTGGGGCCATTTTCATTTTAG; ; Clone variation with respect to NM_004105.3; 661 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004105 unedited; TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGCTGCCCTCCCCT; GCGCACTCCCCTCGCTGCCCGGGCCCGGAGCGCAGCGCGGCCGCACAGATTCACAATGTT; GAAAGCCCTTTTCCTAACTATGCTGACTCTGGCGCTGGTCAAGTCACAGGACACCGAAGA; AACCATCACGTACACGCAATGCACTGACGGATATGAGTGGGATCCTGTGAGACAGCAATG; CAAAGATATTGATGAATGTGACATTGTCCCAGACGCTTGTAAAGGTGGAATGAAGTGTGT; CAACCACTATGGAGGATACCTCTGCCTTCCGAAAACAGCCCAGATTATTGTCAATAATGA; ACAGCCTCAGCAGGAAACACAACCAGCAGAAGGAACCTCAGGGGCAACCACCGGGGTTGT; AGCTGCCAGCAGCATGGCAACCAGTGGAGTGTTGCCCGGGGGTGGTTTTGTGGCCAGTGC; TGCTGCAGTCGCAGGCCCTGAAATGCAGACTGGCCGAAATAACTTTGTCATCCGGCGGAA; CCCAGCTGACCCTCAGCGCATTCCCTCCAACCCTTCCCACCGTATCCAGTGTGCAGCAGG; CTACGAGCAAAGTGAACACAACGTGTGCCAAGACATAGACGAGTGCACTGCANGGACGCA; CAACTGTAGAGCAGACCAAGTGTGCATCAATTACGGGGATCCTTTGCATGTCAGTGCCCT; CCTGGATATCANAAGCGAGGGGAGCAGTGCGTAAACATAGATGAATGTACCATCTCTTCA; TATTGCCACCAAAGATGCGTGAATACACCANGCTCATTTTATTTGCAGTGCAGTCCTGGG; TTTCAATTGGCAGCAACAACTATACCTGCGTAGATATAATGAATGGGATGCCAGCAATCA; TGTGCTCAGCAGTGCTACAACATTCTTGTTCATCATCTGTCATGCAAN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004105 unedited; TGACCGCGGCCGCATCTATAGTCGGTTTTTTTTTTTTTTTTTTCTAAAGAAATTATCTTT; ATTGACCTTTTCCATTTTCTTACAAAGCAGTTAAAAACTCATTCTTACCCTTGCATGCTA; TTCAATGGTTATGATGGCTGCCTCCTTATGAGACTGTTAGTGGAGCTCTAGTAAGTTTCC; TTAAGCACCACAGAAGATCATCATTGCTTGGTCCCACTTTTATAGGAAAGAATCCAAGTT; TCACCAGATAGTGTATACTTAGCTCTCTTGAAGAAGACCAGTTTAGTGGATTAATGTCTA; ATTTACATATAGTAATAAAGACAAACTTTGAATCTTTACATATTAAATGCCCACTTTATA; CCATGGTGTAATTGTTTGAATTATGGGTGAGTGTACAGTATAGAGATGTAGATGCACTAG; ATATATCTATAAATAAAATAGGGCTTTAAGGTAACAATATTCTTTGGCTGACTTAAATGC; CTGTGGTTGACTCTTAGAAAAGACTAAAATGAAAATGGCCCCACTATTATTGTCAATCTT; AACACAGAGCTTGTGCGGAAGGTCCCTATACTGCTGACTGTCAGCATCTCCAGGTCCACG; ATATGTTCTCTTGGTCCTGATAATGACTTCACGAGCACAAGCATTGCACTTACAGGACTT; GTTTGTCGTAGGTAGAACTCTCCATTTTCATTTCCAGATTTAATCCCGAAAGTATTGATG; GGTGTGGCATAAATAGTTGTAGCCCTGTATCTGGAAAATGTCTGATGCACANACCTATCA; AATCGGATGCTCATGTATTTGTANACTATTGACTGGGGCAGTTCTGGCACATGGCATTTG; AGACTGGGCAAACACATCGGTCCTTTGGGGTAAAATGTAGGGATCTGACAGGNATTTCCT; GGGTAACACCNAAGCCGCCTGGAATTTCCACCATTTATTCTCCCCGCGTTCCTTGGGGGC; AAACCCATATTTATTGGATGTGTTCCCTTCACCCCTGGGNTNCCGGGGGGCCCTCTGGAA; TATTCACGGTCTTGA;
• Restriction Sites: NotI-NotI
• ACCN: NM_004105
• Insert Size: 2050 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004105.2, NP_004096.2
• RefSeq Size: 2742 bp
• RefSeq ORF: 1482 bp
• Locus ID: 2202
• Cytogenetics: 2p16.1
• Domains: EGF, EGF_CA
• Protein Families: Secreted Protein
• Summary: This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.[provided by RefSeq, Nov 2009]; Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein.