14 3 3 eta (YWHAH) (NM_003405) Human Untagged Clone

CAT#: SC126632

YWHAH (untagged)-Human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide (YWHAH)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: 14 3 3 eta
• Synonyms: YWHA1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126632 sequence for NM_003405 edited (data generated by NextGen Sequencing)
  ATGGGGGACCGGGAGCAGCTGCTGCAGCGGGCGCGGCTGGCCGAGCAGGCGGAGCGCTAC
  GACGACATGGCCTCCGCTATGAAGGCGGTGACAGAGCTGAATGAACCTCTCTCCAATGAA
  GATCGAAATCTCCTCTCTGTGGCCTACAAGAATGTGGTTGGTGCCAGGCGATCTTCCTGG
  AGGGTCATTAGCAGCATTGAGCAGAAAACCATGGCTGATGGAAACGAAAAGAAATTGGAG
  AAAGTTAAAGCTTACCGGGAGAAGATTGAGAAGGAGCTGGAGACAGTTTGCAATGATGTC
  CTGTCTCTGCTTGACAAGTTCCTGATCAAGAACTGCAATGATTTCCAGTATGAGAGCAAG
  GTGTTTTACCTGAAAATGAAGGGTGATTACTACCGCTACTTAGCAGAGGTCGCTTCTGGG
  GAGAAGAAAAACAGTGTGGTCGAAGCTTCTGAAGCTGCCTACAAGGAAGCCTTTGAAATC
  AGCAAAGAGCAGATGCAACCCACGCATCCCATCCGGCTGGGCCTGGCCCTCAACTTCTCC
  GTGTTCTACTATGAGATCCAGAATGCACCTGAGCAAGCCTGCCTCTTAGCCAAACAAGCC
  TTCGATGATGCCATAGCTGAGCTGGACACACTAAACGAGGATTCCTATAAGGACTCCACG
  CTGATCATGCAGTTGCTGCGAGACAACCTCACCCTCTGGACGAGCGACCAGCAGGATGAA
  GAAGCAGGAGAAGGCAACTGA
  
  Clone variation with respect to NM_003405.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003405 unedited
  TTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGACAGCGCGCGGGGCGAG
  CCAGCGAGAGGGCGCGAGCGGCGGCGCTGCCTGCAGCCTGCAGCCTGCAGCCTCCGGCCG
  GCCGGCGAGCCAGTGCGCGTGCGCGGCGGCGGCCTCCGCAGCGACCGGGGAGCGGACTGA
  CCGGCGGGAGGGCTAGCGAGCCAGCGGTGTGAGGCGCGAGGCGAGGCCGAGCCGCGAGCG
  ACATGGGGGACCGGGAGCAGCTGCTGCAGCGGGCGCGGCTGGCCGAGCAGGCGGAGCGCT
  ACGACGACATGGCCTCCGCTATGAAGGCGGTGACAGAGCTGAATGAACCTCTCTCCAATG
  AAGATCGAAATCTCCTCTCTGTGGCCTACAAGAATGTGGTTGGTGCCAGGCGATCTTCCT
  GGAGGGTCATTAGCAGCATTGAGCAGAAAACCATGGCTGATGGAAACGAAAAGAAATTGG
  AGAAAGTTAAAGCTTACCGGGAGAAGATTGAGAAGGAGCTGGAGACAGTTTGCAATGATG
  TCCTGTCTCTGCTTGACAAGTTCCTGATCAAGAACTGCAATGATTTCCAGTATGAGAGCA
  AGGTGTTTTACCTGAAAATGAAGGGTGATTACTACCGCTACTTANCAGAGGTCGCTTCTG
  GGGAGAAGAAAAACAGTGTGGTCGAAGCTTCTGAAGCTGCCTACAGGGAAGCCTTTGAAA
  TCAGCAAGAGCAGATGCAACCCACGCATTCCATCCGGGCTGGCCCTGGCCCTAAACTTCT
  CCGGGGGTCTCTATGAGATCCAGAATGCCCCTGAGCAAGCCTTGCTCCTTACAAAACAGC
  CTTCGATGATGGCATAGCTGACCTGGACCACTTAAGAAGGATCCTATAGGGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003405 unedited
  TCGCAGCGCATTTTTTACCCATTGATTCCAAAACATTTATTTTAGAAGTCTGGCATTTCA
  TAATCACCCATCTTGATTGACATGGAGCTGACACACATGGCGCTGTCAGGATACACAAGG
  ACAATAGCCAAAGAGTTACAAAAAATAAATCCATGATTCTTAAACAATTGCAACCAAAAA
  AAAAAAAAAGTTACAAGTATCAAAACTTTCAGATGCATTGCATTGAAAAGAAGGTTTGTG
  CACGTCATAATTTAAAGAGGCAAAACCAAGGAGCTACTGAAACCTCACGTTAAACAGTTT
  ATTATAAAGCTGATGGAAAGGAGCAAGTTGTCTCTCTGTATCAGCTTCCCTTAACAGTTT
  TCCATTAATTGAAGAAAGAGGTGGGAGGGGTGAATTCATTTTTGCATGCACAAGATGTAC
  TGCTTAACGAAACACTATCAGCTTGTTTTAAATGGATCTTTTAAATATCAACTGTAGCCT
  GGTTGGCTAATTCTTTCTAATCTTCCCCATTACTTTCGCCTAGATTTCCCATAGATCAAC
  AGGCATAGTAAAATGCCTCATCAGAACACACTTCTCCACACAATTCAAAAAGGGAGCTCC
  TGTGGGCTCAAAGCAACCATCAGTCCAGCAATGCCCATGATTTATCTGAAACTGCTTCCC
  AAGAGACAGGAGTGCAGATCTGAGTAGCTGTGCTGCCAATACAGATAGGTTTAGCACTAG
  ATATTTAGTGATTGTGGCAAGGAAGAATCGGTGATGATGGGGGTGGTGGGTGAAGGAAGG
  GCCAGGGGACCTGAAGGATCTTCAGTTGCCTTCTNCTGCTTCTTCATCCTGCTGGTCGCT
  CGTCCAGAGGGTGAAGTTGTCTCGCAGCACTGCATGATCAGCGTGGAGTCCTTTATAGAA
  ATCCCGNNTTATGTGTCCAGCTCANCTATGCATCATCCAAAGCTTGNNTTGCTAAAAGCA
  GCCTGCTCAGTGCATN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003405
• Insert Size: 1840 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003405.3, NP_003396.1
• RefSeq Size: 1807 bp
• RefSeq ORF: 741 bp
• Locus ID: 7533
• UniProt ID: Q04917
• Cytogenetics: 22q12.3
• Domains: 14-3-3
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Cell cycle, Neurotrophin signaling pathway, Oocyte meiosis
• Gene Summary: This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder. [provided by RefSeq, Jun 2009]