CHRNA3 (BC006114) Human Untagged Clone

CAT#: SC126406

CHRNA3 (untagged)-Human cholinergic receptor, nicotinic, alpha polypeptide 3, mRNA (cDNA clone MGC:12991 IMAGE:3503197), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHRNA3
• Synonyms: Cholinergic receptor, neuronal nicotinic, alpha polypeptide-3; cholinergic receptor, nicotinic, alpha 3; cholinergic receptor, nicotinic, alpha polypeptide 3; LNCR2; MGC104879; neuronal nicotinic acetylcholine receptor, alpha3 subunit; PAOD2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC006114 edited
  GTCCTTCCCAGGGTGTGTTAGCGTCTCTCCTACGGCGCCCGCCTAGCCCGGTGGTCCCAA
  CCCCCTGCGGGAGCTGCACACGCCCCGGAAACCTGGGACAGAAACTGAGTCCCTCTCCTT
  CCTGGTGGTGGTGACAGCACCTGCTCAGATCTGGTCGGACGCCGCCGGCCGGAGCACCCA
  GCCCGGCGGAGAAGGAGCTCGCCCGGCGCTGGGGACTGGGACCTGGAGCCCCTTCCCCTA
  CCGCACGTACGCCCCGCCCCGCGCACGCCCGCCCGCCCGCCCGCGCCTGGCGCAGCTTCA
  CTCCGGATGGTTCCTGTCCTCCCGCGGGTCCGAGGGCGCTGGAAACCCAGCGGCGGCGAA
  GCGGAGAGGAGCCCCGCGCGTCTCCGCCCGCACGGCTCCAGGTCTGGGGTCTGCGCTGGA
  GCCGCGCGGGGAGAGGCCGTCTCTGCGACCGCCGCGCCCGCTCCCGACCGTCCGGGTCCG
  CGGCCAGCCCGGCCACCAGCCATGGGCTCTGGCCCGCTCTCGCTGCCCCTGGCGCTGTCG
  CCGCCGCGGCTGCTGCTGCTGCTGCTGCTGTCTCTGCTGCCAGTGGCCAGGGCCTCAGAG
  GCTGAGCACCGTCTATTTGAGCGGCTGTTTGAAGATTACAATGAGATCATCCGGCCTGTA
  GCCAACGTGTCTGACCCAGTCATCATCCATTTCGAGGTGTCCATGTCTCAGCTGGTGAAG
  GTGGATGAAGTAAACCAGATCATGGAGACCAACCTGTGGCTCAAGCAAATCTGGAATGAC
  TACAAGCTGAAATGGAACCCCTCTGACTATGGTGGGGCAGAGTTCATGCGTGTCCCTGCA
  CAGAAGATCTGGAAGCCAGACATTGTGCTGTATAACAATGCTGTTGGGGATTTCCAGGTG
  GACGACAAGACCAAAGCCTTACTCAAGTACACTGGGGAGGTGACTTGGATACCTCCGGCC
  ATCTTTAAGAGCTCCTGTAAAATCGACGTGACCTACTTCCCGTTTGATTACCAAAACTGT
  ACCATGAAGTTCGGTTCCTGGTCCTACGATAAGGCGAAAATCGATCTGGTCCTGATCGGC
  TCTTCCATGAACCTCAAGGACTATTGGGAGAGCGGCGAGTGGGCCATCATCAAAGCCCCA
  GGCTACAAACACGACATCAAGTACAACTGCTGCGAGGAGATCTACCCCGACATCACATAC
  TCGCTGTACATCCGGCGCCTGCCCTTGTTCTACACCATCAACCTCATCATCCCCTGCCTG
  CTCATCTCCTTCCTCACTGTGCTCGTCTTCTACCTGCCCTCCGACTGCGGTGAGAAGGTG
  ACCCTGTGCATTTCTGTCCTCCTCTCCCTGACGGTGTTTCTCCTGGTGATCACTGAGACC
  ATCCCTTCCACCTCGCTGGTCATCCCCCTGATTGGAGAGTACCTCCTGTTCACCATGATT
  TTTGTAACCTTGTCCATCGTCATCACCGTCTTCGTGCTCAACGTGCACTACAGAACCCCG
  ACGACACACACAATGCCCTCATGGGTGAAGACTGTATTCTTGAACCTGCTCCCCAGGGTC
  ATGTTCATGACCAGGCCAACAAGCAACGAGGGCAACGCTCAGAAGCCGAGGCCCCTCTAC
  GGTGCCGAGCTCTCAAATCTGAATTGCTTCAGCCGCGCAGAGTCCAAAGGCTGCAAGGAG
  GGCTACCCCTGCCAGGACGGGATGTGTGGTTACTGCCACCACCGCAGGATAAAAATCTCC
  AATTTCAGTGCTAACCTCACGAGAAGCTCTAGTTCTGAATCTGTTGATGCTGTGCTGTCC
  CTCTCTGCTTTGTCACCAGAAATCAAAGAAGCCATCCAAAGTGTCAAGTATATTGCTGAA
  AATATGAAAGCACAAAATGAAGCCAAAGAGGAACAAAAAGCCCAAGAGATCCAACAATTG
  AAACGAAAAGAAAAGTCCACAGAAACATCCGATCAAGAACCTGGGCTATGAATTTCCAAT
  CTTCAACAACCTGTTAGGAAAAAAATAAAAATGCATTAAATAATTGTAAAAAAAAAAAAA
  AAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC006114 unedited
  NAAGGTCGNCATTTGTATACGACTCATATAGGCGGACCGCGAATTCGCACGAGGTCCTTC
  CCAGGTGTGTTAGCGTCTCTCCTACGGCGCCCGCCTAGCCCGGTGGTCCCAACCCCCTGC
  GGGAGCTGCACACGCCCCGGAAACCTGGGACAGAAACTGAGTCCCTCTCCTTCCTGGTGG
  TGGTGACAGCACCTGCTCAGATCTGGTCGGACGCCGCCGGCCGGAGCACCCAGCCCGGCG
  GAGAAGGAGCTCGCCCGGCGCTGGGGACTGGGACCTGGAGCCCCTTCCCCTACCGCACGT
  ACGCCCCGCCCCGCGCACGCCCGCCCGCCCGCCCGCGCCTGGCGCAGCTTCACTCCGGAT
  GGTTCCTGTCCTCCCGCGGGTCCGAGGGCGCTGGAAACCCAGCGGCGGCGAAGCGGAGAG
  GAGCCCCGCGCGTCTCCGCCCGCACGGCTCCAGGTCTGGGGTCTGCGCTGGAGCCGCGCG
  GGGAGAGGCCGTCTCTGCGACCGCCGCGCCCGCTCCCGACCGTCCGGGTCCGCGGCCAGC
  CCGGCCACCAGCCATGGGCTCTGGCCCGCTCTCGCTGCCCCTGGCGCTGTCGCCGCCGCG
  GCTGCTGCTGCTGCTGCTGCTGTCTCTGCTGCCAGTGGCCAGGGCCTCAGAGGCTGAGCA
  CCGTCTATTTGAGCGGCTGTTTGAAGATTACAATGAGATCATCCGGCCTGTAGCCAACGT
  GTCTGACCCAGTCATCATCCATTTCGAGGTGTCCATGTCTCAGCTGGTGAAGGTGGATGA
  AGTAAACCAGATCATGGAGACCAACCTGTGGCTCAAGCAAATCTGGNAATGACTACAGCT
  GAAATGGAACCCCTCTGACTATGGTGGGGCAGAGTTCATGCGTGTCCCTGCACAGAAGAT
  CTGGAAGCCAGA
  
• Restriction Sites: Please inquire
• ACCN: BC006114
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC006114.1, AAH06114.1
• RefSeq Size: 2046 bp
• RefSeq ORF: 1467 bp
• Locus ID: 1136
• Cytogenetics: 15q25.1
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Gene Summary: This locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2009]