ARFGAP1 (NM_175609) Human Untagged Clone

CAT#: SC126067

ARFGAP1 (untagged)-Human ADP-ribosylation factor GTPase activating protein 1 (ARFGAP1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARFGAP1
• Synonyms: ARF1GAP; HRIHFB2281
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_175609 edited
  CGGCCCTACCTCGGCCCTGGCCTGACCCCGGCGGCCCTGCCCGCCCCTCCCTCCAGCATC
  ATGGCCAGCCCAAGAACCAGGAAGGTTCTTAAAGAAGTCAGGGTGCAGGATGAGAACAAC
  GTTTGTTTTGAGTGTGGCGCGTTCAATCCTCAGTGGGTCAGTGTGACCTACGGCATCTGG
  ATCTGCCTGGAGTGCTCGGGGAGACACCGCGGGCTTGGGGTTCACCTCAGCTTTGTGCGC
  TCTGTTACTATGGACAAGTGGAAGGACATTGAGCTTGAGAAGATGAAAGCTGGTGGGAAT
  GCTAAGTTCCGAGAGTTCCTGGAGTCTCAGGAGGATTACGATCCTTGCTGGTCCTTGCAG
  GAGAAGTACAACAGCAGAGCCGCGGCCCTCTTTAGGGATAAGGTGGTCGCTCTGGCCGAA
  GGCAGAGAGTGGTCTCTGGAGTCATCACCTGCCCAGAACTGGACCCCACCTCAGCCCAGG
  ACGCTGCCGTCCATGGTGCACCGAGTCTCTGGCCAGCCGCAGAGTGTGACCGCCTCCTCG
  GACAAGGCTTTTGAAGACTGGCTGAATGATGACCTCGGCTCCTATCAAGGGGCCCAGGGG
  AATCGCTACGTGGGGTTTGGGAACACGCCACCGCCTCAGAAGAAAGAAGATGACTTCCTC
  AACAACGCCATGTCCTCCCTGTACTCGGGCTGGAGCAGCTTCACCACTGGAGCCAGCCGG
  TTTGCCTCGGCAGCCAAGGAGGGCGCTACAAAGTTTGGATCCCAAGCGAGTCAGAAGTTT
  TGGGGTCACAAGCAGCAGCCGGAGCCGGCGTCCGAGCTGGGCCACAGCCTGAACGAGAAC
  GTCCTCAAGCCTGCGCAGGAGAAGGTGAAGGAGGGAAAGATTTTTGATGATGTCTCCAGT
  GGGGTCTCTCAGTTGGCGTCCAAGGGAGTCGGTAGTAAGGGATGGCGGGACGTCACCACC
  TTTTTTTCGGGGAAAGCAGAGGGCCCCTTGGACAGCCCCTCGGAGGGCCACAGTTATCAG
  AACAGCGGTCTGGACCACTTCCAAAACAGCAACATAGACCAGAGCTTCTGGGAGACCTTT
  GGAAGTGCTGAGCCCACCAAGACCCGCAAGTCCCCGAGCAGCGACAGCTGGACGTGCGCG
  GACACCTCCACCGAGAGGAGGAGCTCGGACAGCTGGGAGGTGTGGGGCTCGGCCTCCACC
  AACAGGAACAGCAACAGCGACGGCGGGGAGGGCGGGGAGGGCACCAAGAAGGCAGTGCCG
  CCGGCCGTGCCCACTGATGATGGCTGGGACAACCAGAACTGGTAGGGCCCACTGCGCCCC
  CGTCCCCAGCGCCCCCGGGCGACTTCGTGTTTGCACTCTGCCCTCGTCGTTCCTCCTCCT
  TCCATTTGACCCAAGAATCAGCAACTGCAGTGTGAGGACAGCGTCTCGGGAGGCAGGACC
  CTAGGGAGACCCGGGTGTGCGCCGCCTGCGCGTGGGGAGTCTTCGGTGCGTGGGGGCGGC
  TTGCTGTCCAGCCTGTGTGGGGGCCGTCCCGTCCCACACTCCCCTGGGCATTCTTGGACT
  CAAGGCCGGGGCTCTGCGTGGCTTGCTGGGAGGTGGGCTGCAGCACAGAGGCCTGTGACT
  GCGTTCCAGCGGCCAGTTCACTACGCAGTATCTCTGGGGCCTGGGACCAGCCACGTGCCG
  AGCTGTCAGCGACGTGAGGTGTCCCTTCTCGTTGAGATATTTAACTTTGGTTTTGCTCTA
  GTTCTTTCTTTTTGAAGAGAGTGACTGGAGTGGTAAAGATGGAAATGCTGGAAATGATAC
  TGGCGCTCACGCTGCCATCCGACCACCCTCGGCTCCCGAGTCCACGCCTGCCTGGGCCTG
  TGCTGTCAGACCCGCGTCGGTCGTAACCCTCTGTGGCTCCCCTGCATCAGCACCGTCCCA
  CCACCAAGTTCACCAGGTTCACCAGACACGGCCTCCACAATAGCCACACCCACACCTGAG
  CTGTTCTCAGTGCTGGAACTTGACCATCCTGGAACACCCTGGAAGAAAAAGGAGCGCAGG
  GTGGGCCCTCGGCCCTGATGCAGGAGGGTGCGATAGCGGACGTGGCCAGGCAGGAGGGGC
  CGGGTTCAGGAGCTGAGCAGGGGATGCCTGTGCGTGGTGCCTGGGTCTAGGGAAGCTCCA
  GCCCCAGGATGGGGCTGCCCTGCACACCGGTGCCCGCCACATGCCAACCCTCACCTCCCC
  GAGGACTGGATGATGTGCTGCCACGTGTGACTCGTCTCCCTTGTCTGCCCTGTGTGACCC
  TCAGTCTTGGCCAGCCATGCATGCGCCCGAAGCTCGTGCAGTTTGTACGTGAGGTGCTCT
  CCTCCCTGCCACCATGCTCATCACTCTGGCCTTGGCCATGCTCCCTGGTCACCCCACTTC
  CCGGTCGCCGTCTGCAGCACTCCTGGAGCAGCCTGGGCCCTTCAGCCCCTGTGCTCGTCC
  CACCCTAGGGACTCAGCCACTTGCAGAATAGGATGGGACCGAGATTTCAGCGAGCCCTCC
  TGGCGCCCGGTCCTCCCTGTGGGCACCAGCCCTCTTGGTAGCTGGTGTGGAGGGCCGGTG
  TCCTTGGCTGCCACGGAGGGATTTGATCACCGAAGCAGCCACCTGCTGTAGTTGGACCTG
  AGGTCAGAGGCGGGGCATCAGAGGCTCAAGGTGCTGAGAAGCCACCGGGAAAGCAGCCAG
  CACAAAGGGCCCAGGAAGCCAGCCCCCGAGAGCTGAGCGTGGGGGTCTTTGAGTGTCTTT
  CTCCAAGCTGAGACGT
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_175609 unedited
  GTTAGGATTTGTAATACGACTTCTACTATAGGGCGGACCGCGATTCANATCTGGTACCGG
  TCCGGAATTCCCGGGATCGGCCCTACCTGGGCCCTGGCCTGACCCCGGCGGCCCTGCCCG
  CCCCTCCCTCCAGCATCATGGCCAGCCCAAGAACCAGGAAGGTTCTTAAAGAAGTCAGGG
  TGCAGGATGAGAACAACGTTTGTTTTGAGTGTGGCGCGTTCAATCCTCAGTGGGTCAGTG
  TGACCTACGGCATCTGGATCTGCCTGGAGTGCTCGGGGAGACACCGCGGGCTTGGGGTTC
  ACCTCAGCTTTGTGCGCTCTGTTACTATGGACAAGTGGAAGGACATTGAGCTTGAGAAGA
  TGAAAGCTGGTGGGAATGCTAAGTTCCGAGAGTTCCTGGAGTCTCAGGAGGATTACGATC
  CTTGCTGGTCCTTGCAGGAGAAGTACAACAGCAGAGCCGCGGCCCTCTTTAGGGATAAGG
  TGGTCGCTCTGGCCGAAGGCAGAGAGTGGTCTCTGGAGTCATCACCTGCCCAGAACTGGA
  CCCCACCTCAGCCCAGGACGCTGCCGTCCATGGTGCACCGAGTCTCTGGCCAGCCGCAGA
  GTGTGACCGCCTCCTCGGACAAGGCTTTTGAAGACTGGCTGAATGATGACCTCGGCTCCT
  ATCAAGGGGCCCAGGGGAATCGCTACGTGGGGTTTGGGACACGCCACCGCCTCAGAAGAA
  GGAGATGACTTCCTCAACAACGCCATGTCCTCCCTGTACTCGGGCTGNAGCAGCTTTACC
  ACTGGAGCCAGCCGGTTGCCTCGGCAGCCAAGGAGGGCGCTACAAAGTTGGATCCCCAGC
  GAGTCCACAGTTTTGGGGTACAAGCACAACCCGAGCCGGCGTCCAACTGGGCCCAACCTG
  GACGAGACGTCCTCAGCCTGGCCCAGAGAGGGG
  
• Restriction Sites: Please inquire
• ACCN: NM_175609
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175609.1, NP_783202.1
• RefSeq Size: 3274 bp
• RefSeq ORF: 1245 bp
• Locus ID: 55738
• UniProt ID: Q8N6T3
• Cytogenetics: 20q13.33
• Protein Pathways: Endocytosis
• Gene Summary: The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (2) encodes the longest isoform (b).