KAT2A (NM_021078) Human Untagged Clone

CAT#: SC125929

KAT2A (untagged)-Human K(lysine) acetyltransferase 2A (KAT2A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KAT2A
• Synonyms: GCN5; GCN5L2; hGCN5; PCAF-b
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_021078 edited
  CCACGCGTCCGGGGCTGGGAGCGCGGCGCCGCTCTCCGCTGCGGGGGAGGCCATGGCGGA
  ACCTTCCCAGGCCCCGACCCCGGCCCCGGCTGCGCAGCCCCGGCCCCTTCAGTCCCCAGC
  CCCTGCCCCAACTCCGACTCCTGCACCCAGCCCGGCTTCAGCCCCGATTCCGACTCCCAC
  CCCGGCACCAGCCCCTGCCCCAGCTGCAGCCCCAGCCGGCAGCACAGGGACTGGGGGGCC
  CGGGGTAGGAAGTGGGGGGGCCGGGAGCGGGGGGGATCCGGCTCGACCTGGCCTGAGCCA
  GCAGCAGCGCGCCAGTCAGAGGAAGGCGCAAGTCCGGGGGCTGCCGCGCGCCAAGAAGCT
  TGAGAAGCTAGGGGTCTTCTCGGCTTGCAAGGCCAATGAAACCTGTAAGTGTAATGGCTG
  GAAAAACCCCAAGCCCCCCACTGCACCCCGCATGGATCTGCAGCAGCCAGCTGCCAACCT
  GAGTGAGCTGTGCCGCAGTTGTGAGCACCCCTTGGCTGACCACGTATCCCACTTGGAGAA
  TGTGTCAGAGGATGAGATAAACCGACTGCTGGGGATGGTGGTGGATGTGGAGAATCTCTT
  CATGTCTGTTCACAAGGAAGAGGACACAGACACCAAGCAGGTCTATTTCTACCTCTTCAA
  GCTACTGCGGAAATGCATCCTGCAGATGACCCGGCCTGTGGTGGAGGGGTCCCTGGGCAG
  CCCTCCATTTGAGAAACCTAATATTGAGCAGGGTGTGCTGAACTTTGTGCAGTACAAGTT
  TAGTCACCTGGCTCCCCGGGAGCGGCAGACGATGTTCGAGCTCTCAAAGATGTTCTTGCT
  CTGCCTTAACTACTGGAAGCTTGAGACACCTGCCCAGTTTCGGCAGAGGTCTCAGGCTGA
  GGACGTGGCTACCTACAAGGTCAATTACACCAGATGGCTCTGTTACTGCCACGTGCCCCA
  GAGCTGTGATAGCCTCCCCCGCTACGAAACCACTCATGTCTTTGGGCGAAGCCTTCTCCG
  GTCCATTTTCACCGTTACCCGCCGGCAGCTGCTGGAAAAGTTCCGAGTGGAGAAGGACAA
  ATTGGTGCCCGAGAAGAGGACCCTCATCCTCACTCACTTCCCCAAATTCCTGTCCATGCT
  GGAGGAGGAGATCTATGGGGCAAACTCTCCAATCTGGGAGTCAGGCTTCACCATGCCACC
  CTCAGAGGGGACACAGCTGGTTCCCCGGCCAGCTTCAGTCAGTGCAGCGGTTGTTCCCAG
  CACCCCCATCTTCAGCCCCAGCATGGGTGGGGGCAGCAACAGCTCCCTGAGTCTGGATTC
  TGCAGGGGCCGAGCCTATGCCAGGCGAGAAGAGGACGCTCCCAGAGAACCTGACCCTGGA
  GGATGCCAAGCGGCTCCGTGTGATGGGTGACATCCCCATGGAGCTGGTCAATGAGGTCAT
  GCTGACCATCACTGACCCTGCTGCCATGCTGGGGCCTGAGACGAGCCTGCTTTCGGCCAA
  TGCGGCCCGGGATGAGACAGCCCGCCTGGAGGAGCGCCGCGGCATCATCGAGTTCCATGT
  CATCGGCAACTCACTGACGCCCAAGGCCAACCGGCGGGTGTTGCTGTGGCTCGTGGGGCT
  GCAGAATGTCTTTTCCCACCAGCTGCCGCGCATGCCTAAGGAGTATATCGCCCGCCTCGT
  CTTTGACCCGAAGCACAAGACTCTGGCCTTGATCAAGGATGGGCGGGTCATCGGTGGCAT
  CTGCTTCCGCATGTTTCCCACCCAGGGCTTCACGGAGATTGTCTTCTGTGCTGTCACCTC
  GAATGAGCAGGTCAAGGGTTATGGGACCCACCTGATGAACCACCTGAAGGAGTATCACAT
  CAAGCACAACATTCTCTACTTCCTCACCTACGCCGACGAGTACGCCATCGGCTACTTCAA
  AAAGCAGGGTTTCTCCAAGGACATCAAGGTGCCCAAGAGCCGCTACCTGGGCTACATCAA
  GGACTACGAGGGAGCGACGCTGATGGAGTGTGAGCTGAATCCCCGCATCCCCTACACGGA
  GCTGTCCCACATCATCAAGAAGCAGAAAGAGATCATCAAGAAGCTGATTGAGCGCAAACA
  GGCCCAGATCCGCAAGGTCTACCCGGGGCTCAGCTGCTTCAAGGAGGGCGTGAGGCAGAT
  CCCTGTGGAGAGCGTTCCTGGCATTCGAGAGACAGGCTGGAAGCCATTGGGGAAGGAGAA
  GGGGAAGGAGCTGAAGGACCCCGACCAGCTCTACACAACCCTCAAAAACCTGCTGGCCCA
  AATCAAGTCTCACCCCAGTGCCTGGCCCTTCATGGAGCCTGTGAAGAAGTCGGAGGCCCC
  TGACTACTACGAGGTCATCCGCTTCCCCATTGACCTGAAGACCATGACTGAGCGGCTGCG
  AAGCCGCTACTACGTGACCCGGAAGCTCTTTGTGGCCGACCTGCAGCGGGTCATCGCCAA
  CTGTCGCGAGTACAACCCCCCGGACAGCGAGTACTGCCGCTGTGCCAGCGCCCTGGAGAA
  GTTCTTCTACTTCAAGCTCAAGGAGGGAGGCCTCATTGACAAGTAGGCCCATCTTTGGGC
  CGCAGCCCTGACCTGGAATGTCTCCACCTCGGATTCTGATCTGATCCTTAGGGGGTGCCC
  TGGCCCCACGGACCCGACTCAGCTTGAGACACTCCAGCCAAGGGTCCTCCGGACCCGATC
  CTGCAGCTCTTTCTGGACCTTCAGGCACCCCCAAGCGTGCAGCTCTGTCCCAGCCTTCAC
  TGTGTGTGAGAGGTCTCCTGGGTTGGGGCCCAGCCCCTCTAGAGTAGCTGGTGGCCAGGG
  ATGAACCTTGCCCAGCCGTGGTGGCCCCCAGGCCTGGTCCCCAAGAGCTTTGGAGGCTTG
  GATTCCTGGGCCTGGCCCAGGTGGCTGTTTCCCTGAGGACCAGAACTGCTCATTTTAGCT
  TGAGTGATGGCTTCAGGGGTTGGAAGTTCAGCCCAAACTGAAGGGGGCCATGCCTTGTCC
  AGCACTGTTCTGTCAGTCTCCCCCAGGGGTGGGGGGTATGGGGACCATTCATTCCCTGGC
  ATTAATCCCTTAGAGGGAATAATAAAGCTTTTTATTTCTCTGTGAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021078 unedited
  GGTTAGGATTTGTATACGACTTACTATAGGCGGCCGCGGATTCAGATCTGGTACCGGTCC
  GGAATTCCCGGGATATCGTCGACCCACGCGTCCGGGGCTGGGAGCGCGGCGCCGCTCTCC
  GCTGCGGGGGAGGCCATGGCGGAACCTTCCCAGGCCCCGACCCCGGCCCCGGCTGCGCAG
  CCCCGGCCCCTTCAGTCCCCAGCCCCTGCCCCAACTCCGACTCCTGCACCCAGCCCGGCT
  TCAGCCCCGATTCCGACTCCCACCCCGGCACCAGCCCCTGCCCCAGCTGCAGCCCCAGCC
  GGCAGCACAGGGACTGGGGGGCCCGGGGTAGGAAGTGGGGGGGCCGGGAGCGGGGGGGAT
  CCGGCTCGACCTGGCCTGAGCCAGCAGCAGCGCGCCAGTCAGAGGAAGGCGCAAGTCCGG
  GGGCTGCCGCGCGCCAAGAAGCTTGAGAAGCTAGGGGTCTTCTCGGCTTGCAAGGCCAAT
  GAAACCTGTAAGTGTAATGGCTGGAAAAACCCCAAGCCCCCCACTGCACCCCGCATGGAT
  CTGCAGCAGCCAGCTGCCAACCTGAGTGAGCTGTGCCGCAGTTGTGAGCACCCCTTGGCT
  GACCACGTATCCCACTTGGAGAATGTGTCAGAGGATGAGATAAACCGACTGCTGGGGATG
  GTGGTGGATGTGGAGAATCTCTTCATGTCTGTTCACAGGAAGAGGACACAGACACCAAGC
  AGGTCTATTTCTACCTCTTCAAGCTACTGCGGAAATGCATCCTGCAGATGACCCGGCCTG
  TGGTGGAGGGGTCCCTGGGCAGCCCTCCTTTTGAGAACCCAATATTGAGCAGGGTGTGCT
  GAACTTTGGGCCAGACAGGTTAGTCACCTGGCCTCCCGGAAGCGGCANACATGTTCCAGC
  CTCTAAAAAGTTCTTGCTCTGCCTA
  
• Restriction Sites: Please inquire
• ACCN: NM_021078
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021078.1, NP_066564.1
• RefSeq Size: 3074 bp
• RefSeq ORF: 2514 bp
• Locus ID: 2648
• UniProt ID: Q92830
• Cytogenetics: 17q21.2
• Protein Families: Transcription Factors
• Protein Pathways: Notch signaling pathway
• Gene Summary: KAT2A, or GCN5, is a histone acetyltransferase (HAT) that functions primarily as a transcriptional activator. It also functions as a repressor of NF-kappa-B (see MIM 164011) by promoting ubiquitination of the NF-kappa-B subunit RELA (MIM 164014) in a HAT-independent manner (Mao et al., 2009 [PubMed 19339690]).[supplied by OMIM, Sep 2009]