GAA (NM_000152) Human Untagged Clone

SKU SC125512

GAA (untagged)-Human glucosidase, alpha, acid (GAA), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: GAA
• Synonyms: LYAG
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125512 sequence for NM_000152 edited (data generated by NextGen Sequencing); ATGGGAGTGAGGCACCCGCCCTGCTCCCACCGGCTCCTGGCCGTCTGCGCCCTCGTGTCC; TTGGCAACCGCTGCACTCCTGGGGCACATCCTACTCCATGATTTCCTGCTGGTTCCCCGA; GAGCTGAGTGGCTCCTCCCCAGTCCTGGAGGAGACTCACCCAGCTCACCAGCAGGGAGCC; AGCAGACCAGGGCCCCGGGATGCCCAGGCACACCCCGGCCGTCCCAGAGCAGTGCCCACA; CAGTGCGACGTCCCCCCCAACAGCCGCTTCGATTGCGCCCCTGACAAGGCCATCACCCAG; GAACAGTGCGAGGCCCGCGGCTGTTGCTACATCCCTGCAAAGCAGGGGCTGCAGGGAGCC; CAGATGGGGCAGCCCTGGTGCTTCTTCCCACCCAGCTACCCCAGCTACAAGCTGGAGAAC; CTGAGCTCCTCTGAAATGGGCTACACGGCCACCCTGACCCGTACCACCCCCACCTTCTTC; CCCAAGGACATCCTGACCCTGCGGCTGGACGTGATGATGGAGACTGAGAACCGCCTCCAC; TTCACGATCAAAGATCCAGCTAACAGGCGCTACGAGGTGCCCTTGGAGACCCCGCATGTC; CACAGCCGGGCACCGTCCCCACTCTACAGCGTGGAGTTCTCCGAGGAGCCCTTCGGGGTG; ATCGTGCGCCGGCAGCTGGACGGCCGCGTGCTGCTGAACACGACGGTGGCGCCCCTGTTC; TTTGCGGACCAGTTCCTTCAGCTGTCCACCTCGCTGCCCTCGCAGTATATCACAGGCCTC; GCCGAGCACCTCAGTCCCCTGATGCTCAGCACCAGCTGGACCAGGATCACCCTGTGGAAC; CGGGACCTTGCGCCCACGCCCGGTGCGAACCTCTACGGGTCTCACCCTTTCTACCTGGCG; CTGGAGGACGGCGGGTCGGCACACGGGGTGTTCCTGCTAAACAGCAATGCCATGGATGTG; GTCCTGCAGCCGAGCCCTGCCCTTAGCTGGAGGTCGACAGGTGGGATCCTGGATGTCTAC; ATCTTCCTGGGCCCAGAGCCCAAGAGCGTGGTGCAGCAGTACCTGGACGTTGTGGGATAC; CCGTTCATGCCGCCATACTGGGGCCTGGGCTTCCACCTGTGCCGCTGGGGCTACTCCTCC; ACCGCTATCACCCGCCAGGTGGTGGAGAACATGACCAGGGCCCACTTCCCCCTGGACGTC; CAGTGGAACGACCTGGACTACATGGACTCCCGGAGGGACTTCACGTTCAACAAGGATGGC; TTCCGGGACTTCCCGGCCATGGTGCAGGAGCTGCACCAGGGCGGCCGGCGCTACATGATG; ATCGTGGATCCTGCCATCAGCAGCTCGGGCCCTGCCGGGAGCTACAGGCCCTACGACGAG; GGTCTGCGGAGGGGGGTTTTCATCACCAACGAGACCGGCCAGCCGCTGATTGGGAAGGTA; TGGCCCGGGTCCACTGCCTTCCCCGACTTCACCAACCCCACAGCCCTGGCCTGGTGGGAG; GACATGGTGGCTGAGTTCCATGACCAGGTGCCCTTCGACGGCATGTGGATTGACATGAAC; GAGCCTTCCAACTTCATCAGGGGCTCTGAGGACGGCTGCCCCAACAATGAGCTGGAGAAC; CCACCCTACGTGCCTGGGGTGGTTGGGGGGACCCTCCAGGCGGCCACCATCTGTGCCTCC; AGCCACCAGTTTCTCTCCACACACTACAACCTGCACAACCTCTACGGCCTGACCGAAGCC; ATCGCCTCCCACAGGGCGCTGGTGAAGGCTCGGGGGACACGCCCATTTGTGATCTCCCGC; TCGACCTTTGCTGGCCACGGCCGATACGCCGGCCACTGGACGGGGGACGTGTGGAGCTCC; TGGGAGCAGCTCGCCTCCTCCGTGCCAGAAATCCTGCAGTTTAACCTGCTGGGGGTGCCT; CTGGTCGGGGCCGACGTCTGCGGCTTCCTGGGCAACACCTCAGAGGAGCTGTGTGTGCGC; TGGACCCAGCTGGGGGCCTTCTACCCCTTCATGCGGAACCACAACAGCCTGCTCAGTCTG; CCCCAGGAGCCGTACAGCTTCAGCGAGCCGGCCCAGCAGGCCATGAGGAAGGCCCTCACC; CTGCGCTACGCACTCCTCCCCCACCTCTACACACTGTTCCACCAGGCCCACGTCGCGGGG; GAGACCGTGGCCCGGCCCCTCTTCCTGGAGTTCCCCAAGGACTCTAGCACCTGGACTGTG; GACCACCAGCTCCTGTGGGGGGAGGCCCTGCTCATCACCCCAGTGCTCCAGGCCGGGAAG; GCCGAAGTGACTGGCTACTTCCCCTTGGGCACATGGTACGACCTGCAGACGGTGCCAGTA; GAGGCCCTTGGCAGCCTCCCACCCCCACCTGCAGCTCCCCGTGAGCCAGCCATCCACAGC; GAGGGGCAGTGGGTGACGCTGCCGGCCCCCCTGGACACCATCAACGTCCACCTCCGGGCT; GGGTACATCATCCCCCTGCAGGGCCCTGGCCTCACAACCACAGAGTCCCGCCAGCAGCCC; ATGGCCCTGGCTGTGGCCCTGACCAAGGGTGGGGAGGCCCGAGGGGAGCTGTTCTGGGAC; GATGGAGAGAGCCTGGAAGTGCTGGAGCGAGGGGCCTACACACAGGTCATCTTCCTGGCC; AGGAATAACACGATCGTGAATGAGCTGGTACGTGTGACCAGTGAGGGAGCTGGCCTGCAG; CTGCAGAAGGTGACTGTCCTGGGCGTGGCCACGGCGCCCCAGCAGGTCCTCTCCAACGGT; GTCCCTGTCTCCAACTTCACCTACAGCCCCGACACCAAGGTCCTGGACATCTGTGTCTCG; CTGTTGATGGGAGAGCAGTTTCTCGTCAGCTGGTGTTAG; ; Clone variation with respect to NM_000152.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000152 unedited; TTTCCCCGCCCGTTGNCGCATTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCA; GAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGA; ATTCGGCACGAGGGTGAGCCGGGCCGGGGCTGCGGGGCTTCCCTGAGCGCGGGCCGGGTC; GGTGGGGCGGTCGGCTGCCCGCGCGGCCTCTCAGTTGGGAAAGCTGAGGTTGTCGCCGGG; GCCGCGGGTGGAGGTCGGGGATGAGGCAGCAGGTAGGACAGTGACCTCGGTGACGCGAAG; GACCCCGGCCACCTCTAGGTTCTCCTCGTCCGCCCGTTGTTCAGCGAGGGAGGCTCTGCG; CGTGCCGCAGCTGACGGGGAAACTGAGGCACGGAGCGGGCCTGTAGGAGCTGTCCAGGCC; ATCTCCAACCATGGGAGTGAGGCACCCGCCCTGCTCCCACCGGCTCCTGGCCGTCTGCGC; CCTCGTGTCCTTGGCAACCGCTGCACTCCTGGGGCACATCCTACTCCATGATTTCCTGCT; GGTTCCCCGAGAGCTGAGTGGCTCCTCCCCAGTCCTGGAGGAGACTCACCCAGCTCACCA; GCAGGGAGCCAGCAGACCAGGGCCCNCGGGATGCCCAGGCACACCCCGGGCCGTCCCAGA; GCAGTGCCCACACAGTGCGACGTCCCCCCCACAGCCGCTTCGATTGCGCCCCTGACANGG; CCATCACCCAGNAACAGTGCGAGGCCCCGCGGCTGGTGCTACATCCCTGNCAAGCAGGGG; CTGCAGGGAGCCCANATGGGGCAGCCTGGTGCTTTTTCCACCAGCTACCCAGCTACAGCT; GGAGAACTGAGCTCTCTGAATGGGCTACACGGCACCCTGACCGTACACCCCCACTTCTTC; CCAGGACANCTGACCTGCGNNTGACGTGA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000152 unedited; TCNCCCCCAAGATCGTGGNACGCGGNCCCGCATTTTANGANCGNGTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCAAAGCCCCCTTTTATTAAAAATA; ATTGCACTTAAAATTTAAAATCTCCCAAGTCCTGGGAATTTTCCTGAGTTCCCAGGCTTG; CTCGGGGACCGGCAGGCATCCAGCCCTTTGGGCAGCCGGGCAGCGGTCGCGTTGGGGCAA; AGCAACAGGCCCGAAGCAGGAGCTCAGGTGCATACCCCACACCTCCACCTGAGCACCCCC; TTTTCCGGTGCTGGGAACAAATGGAGGGGGGTGGCTAATACTACCCGCATGCCGGCAACA; GGTGAGGAGGGCAAACCTTACAATCTTATTAACACAGAGCAGCCCTCCAGGGCCCCGGCC; CACAGTGCGATCTAGGGAAAAAGCTCTCCTAAACACGTTGGGGGCCAAAGCCCCGGCCCA; GGAGGGGGAAACAAGCGATGCGGCGGCTTGGAATGGTTAGTGCCCAGCTCCAGGTGACAC; ATGCAACCCCCAGGCCCGCACACAGTTGCTGCCCGCACACAGGCTCTGCTTCCCTGGGGA; ACCAGCCTCCCTCTGGAGAGACTAACACACTCCGCCCGGCTAACACCAGCTGACGAGAAA; CTGCTCTCCCATCAACAGCGAGACACAGATGTTCCAGGACCTTGGTGTCGGGGCTGTAGG; TGAAGTTGGAGACAGGGACACCGTTTGGAAAGGACCTGCTGGGGGCCCCCGGCCCACCCC; CAAGAAGTCACCTTCTGGAGGTGCAGGCCAACTCCCTTACTGGTCAAACGTACCAACTTA; TTCAGAATCGGGTTATTCCCTGGCCGA;
• Restriction Sites: NotI-NotI
• ACCN: NM_000152
• Insert Size: 3700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000152.2, NP_000143.1
• RefSeq Size: 3846 bp
• RefSeq ORF: 2859 bp
• Locus ID: 2548
• UniProt ID: P10253
• Cytogenetics: 17q25.3
• Domains: Glyco_hydro_31, PD
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Galactose metabolism, Lysosome, Metabolic pathways, Starch and sucrose metabolism
• Summary: This gene encodes lysosomal alpha-glucosidase, which is essential for the degradation of glycogen to glucose in lysosomes. The encoded preproprotein is proteolytically processed to generate multiple intermediate forms and the mature form of the enzyme. Defects in this gene are the cause of glycogen storage disease II, also known as Pompe's disease, which is an autosomal recessive disorder with a broad clinical spectrum. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]; Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, and 3 all encode the same protein.