GAA (NM_000152) Human Untagged Clone

CAT#: SC125512

GAA (untagged)-Human glucosidase, alpha, acid (GAA), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GAA
• Synonyms: LYAG
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125512 sequence for NM_000152 edited (data generated by NextGen Sequencing)
  ATGGGAGTGAGGCACCCGCCCTGCTCCCACCGGCTCCTGGCCGTCTGCGCCCTCGTGTCC
  TTGGCAACCGCTGCACTCCTGGGGCACATCCTACTCCATGATTTCCTGCTGGTTCCCCGA
  GAGCTGAGTGGCTCCTCCCCAGTCCTGGAGGAGACTCACCCAGCTCACCAGCAGGGAGCC
  AGCAGACCAGGGCCCCGGGATGCCCAGGCACACCCCGGCCGTCCCAGAGCAGTGCCCACA
  CAGTGCGACGTCCCCCCCAACAGCCGCTTCGATTGCGCCCCTGACAAGGCCATCACCCAG
  GAACAGTGCGAGGCCCGCGGCTGTTGCTACATCCCTGCAAAGCAGGGGCTGCAGGGAGCC
  CAGATGGGGCAGCCCTGGTGCTTCTTCCCACCCAGCTACCCCAGCTACAAGCTGGAGAAC
  CTGAGCTCCTCTGAAATGGGCTACACGGCCACCCTGACCCGTACCACCCCCACCTTCTTC
  CCCAAGGACATCCTGACCCTGCGGCTGGACGTGATGATGGAGACTGAGAACCGCCTCCAC
  TTCACGATCAAAGATCCAGCTAACAGGCGCTACGAGGTGCCCTTGGAGACCCCGCATGTC
  CACAGCCGGGCACCGTCCCCACTCTACAGCGTGGAGTTCTCCGAGGAGCCCTTCGGGGTG
  ATCGTGCGCCGGCAGCTGGACGGCCGCGTGCTGCTGAACACGACGGTGGCGCCCCTGTTC
  TTTGCGGACCAGTTCCTTCAGCTGTCCACCTCGCTGCCCTCGCAGTATATCACAGGCCTC
  GCCGAGCACCTCAGTCCCCTGATGCTCAGCACCAGCTGGACCAGGATCACCCTGTGGAAC
  CGGGACCTTGCGCCCACGCCCGGTGCGAACCTCTACGGGTCTCACCCTTTCTACCTGGCG
  CTGGAGGACGGCGGGTCGGCACACGGGGTGTTCCTGCTAAACAGCAATGCCATGGATGTG
  GTCCTGCAGCCGAGCCCTGCCCTTAGCTGGAGGTCGACAGGTGGGATCCTGGATGTCTAC
  ATCTTCCTGGGCCCAGAGCCCAAGAGCGTGGTGCAGCAGTACCTGGACGTTGTGGGATAC
  CCGTTCATGCCGCCATACTGGGGCCTGGGCTTCCACCTGTGCCGCTGGGGCTACTCCTCC
  ACCGCTATCACCCGCCAGGTGGTGGAGAACATGACCAGGGCCCACTTCCCCCTGGACGTC
  CAGTGGAACGACCTGGACTACATGGACTCCCGGAGGGACTTCACGTTCAACAAGGATGGC
  TTCCGGGACTTCCCGGCCATGGTGCAGGAGCTGCACCAGGGCGGCCGGCGCTACATGATG
  ATCGTGGATCCTGCCATCAGCAGCTCGGGCCCTGCCGGGAGCTACAGGCCCTACGACGAG
  GGTCTGCGGAGGGGGGTTTTCATCACCAACGAGACCGGCCAGCCGCTGATTGGGAAGGTA
  TGGCCCGGGTCCACTGCCTTCCCCGACTTCACCAACCCCACAGCCCTGGCCTGGTGGGAG
  GACATGGTGGCTGAGTTCCATGACCAGGTGCCCTTCGACGGCATGTGGATTGACATGAAC
  GAGCCTTCCAACTTCATCAGGGGCTCTGAGGACGGCTGCCCCAACAATGAGCTGGAGAAC
  CCACCCTACGTGCCTGGGGTGGTTGGGGGGACCCTCCAGGCGGCCACCATCTGTGCCTCC
  AGCCACCAGTTTCTCTCCACACACTACAACCTGCACAACCTCTACGGCCTGACCGAAGCC
  ATCGCCTCCCACAGGGCGCTGGTGAAGGCTCGGGGGACACGCCCATTTGTGATCTCCCGC
  TCGACCTTTGCTGGCCACGGCCGATACGCCGGCCACTGGACGGGGGACGTGTGGAGCTCC
  TGGGAGCAGCTCGCCTCCTCCGTGCCAGAAATCCTGCAGTTTAACCTGCTGGGGGTGCCT
  CTGGTCGGGGCCGACGTCTGCGGCTTCCTGGGCAACACCTCAGAGGAGCTGTGTGTGCGC
  TGGACCCAGCTGGGGGCCTTCTACCCCTTCATGCGGAACCACAACAGCCTGCTCAGTCTG
  CCCCAGGAGCCGTACAGCTTCAGCGAGCCGGCCCAGCAGGCCATGAGGAAGGCCCTCACC
  CTGCGCTACGCACTCCTCCCCCACCTCTACACACTGTTCCACCAGGCCCACGTCGCGGGG
  GAGACCGTGGCCCGGCCCCTCTTCCTGGAGTTCCCCAAGGACTCTAGCACCTGGACTGTG
  GACCACCAGCTCCTGTGGGGGGAGGCCCTGCTCATCACCCCAGTGCTCCAGGCCGGGAAG
  GCCGAAGTGACTGGCTACTTCCCCTTGGGCACATGGTACGACCTGCAGACGGTGCCAGTA
  GAGGCCCTTGGCAGCCTCCCACCCCCACCTGCAGCTCCCCGTGAGCCAGCCATCCACAGC
  GAGGGGCAGTGGGTGACGCTGCCGGCCCCCCTGGACACCATCAACGTCCACCTCCGGGCT
  GGGTACATCATCCCCCTGCAGGGCCCTGGCCTCACAACCACAGAGTCCCGCCAGCAGCCC
  ATGGCCCTGGCTGTGGCCCTGACCAAGGGTGGGGAGGCCCGAGGGGAGCTGTTCTGGGAC
  GATGGAGAGAGCCTGGAAGTGCTGGAGCGAGGGGCCTACACACAGGTCATCTTCCTGGCC
  AGGAATAACACGATCGTGAATGAGCTGGTACGTGTGACCAGTGAGGGAGCTGGCCTGCAG
  CTGCAGAAGGTGACTGTCCTGGGCGTGGCCACGGCGCCCCAGCAGGTCCTCTCCAACGGT
  GTCCCTGTCTCCAACTTCACCTACAGCCCCGACACCAAGGTCCTGGACATCTGTGTCTCG
  CTGTTGATGGGAGAGCAGTTTCTCGTCAGCTGGTGTTAG
  
  Clone variation with respect to NM_000152.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000152 unedited
  TTTCCCCGCCCGTTGNCGCATTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCA
  GAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGA
  ATTCGGCACGAGGGTGAGCCGGGCCGGGGCTGCGGGGCTTCCCTGAGCGCGGGCCGGGTC
  GGTGGGGCGGTCGGCTGCCCGCGCGGCCTCTCAGTTGGGAAAGCTGAGGTTGTCGCCGGG
  GCCGCGGGTGGAGGTCGGGGATGAGGCAGCAGGTAGGACAGTGACCTCGGTGACGCGAAG
  GACCCCGGCCACCTCTAGGTTCTCCTCGTCCGCCCGTTGTTCAGCGAGGGAGGCTCTGCG
  CGTGCCGCAGCTGACGGGGAAACTGAGGCACGGAGCGGGCCTGTAGGAGCTGTCCAGGCC
  ATCTCCAACCATGGGAGTGAGGCACCCGCCCTGCTCCCACCGGCTCCTGGCCGTCTGCGC
  CCTCGTGTCCTTGGCAACCGCTGCACTCCTGGGGCACATCCTACTCCATGATTTCCTGCT
  GGTTCCCCGAGAGCTGAGTGGCTCCTCCCCAGTCCTGGAGGAGACTCACCCAGCTCACCA
  GCAGGGAGCCAGCAGACCAGGGCCCNCGGGATGCCCAGGCACACCCCGGGCCGTCCCAGA
  GCAGTGCCCACACAGTGCGACGTCCCCCCCACAGCCGCTTCGATTGCGCCCCTGACANGG
  CCATCACCCAGNAACAGTGCGAGGCCCCGCGGCTGGTGCTACATCCCTGNCAAGCAGGGG
  CTGCAGGGAGCCCANATGGGGCAGCCTGGTGCTTTTTCCACCAGCTACCCAGCTACAGCT
  GGAGAACTGAGCTCTCTGAATGGGCTACACGGCACCCTGACCGTACACCCCCACTTCTTC
  CCAGGACANCTGACCTGCGNNTGACGTGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000152 unedited
  TCNCCCCCAAGATCGTGGNACGCGGNCCCGCATTTTANGANCGNGTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCAAAGCCCCCTTTTATTAAAAATA
  ATTGCACTTAAAATTTAAAATCTCCCAAGTCCTGGGAATTTTCCTGAGTTCCCAGGCTTG
  CTCGGGGACCGGCAGGCATCCAGCCCTTTGGGCAGCCGGGCAGCGGTCGCGTTGGGGCAA
  AGCAACAGGCCCGAAGCAGGAGCTCAGGTGCATACCCCACACCTCCACCTGAGCACCCCC
  TTTTCCGGTGCTGGGAACAAATGGAGGGGGGTGGCTAATACTACCCGCATGCCGGCAACA
  GGTGAGGAGGGCAAACCTTACAATCTTATTAACACAGAGCAGCCCTCCAGGGCCCCGGCC
  CACAGTGCGATCTAGGGAAAAAGCTCTCCTAAACACGTTGGGGGCCAAAGCCCCGGCCCA
  GGAGGGGGAAACAAGCGATGCGGCGGCTTGGAATGGTTAGTGCCCAGCTCCAGGTGACAC
  ATGCAACCCCCAGGCCCGCACACAGTTGCTGCCCGCACACAGGCTCTGCTTCCCTGGGGA
  ACCAGCCTCCCTCTGGAGAGACTAACACACTCCGCCCGGCTAACACCAGCTGACGAGAAA
  CTGCTCTCCCATCAACAGCGAGACACAGATGTTCCAGGACCTTGGTGTCGGGGCTGTAGG
  TGAAGTTGGAGACAGGGACACCGTTTGGAAAGGACCTGCTGGGGGCCCCCGGCCCACCCC
  CAAGAAGTCACCTTCTGGAGGTGCAGGCCAACTCCCTTACTGGTCAAACGTACCAACTTA
  TTCAGAATCGGGTTATTCCCTGGCCGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000152
• Insert Size: 3700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000152.2, NP_000143.1
• RefSeq Size: 3846 bp
• RefSeq ORF: 2859 bp
• Locus ID: 2548
• UniProt ID: P10253
• Cytogenetics: 17q25.3
• Domains: Glyco_hydro_31, PD
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Galactose metabolism, Lysosome, Metabolic pathways, Starch and sucrose metabolism
• Gene Summary: This gene encodes lysosomal alpha-glucosidase, which is essential for the degradation of glycogen to glucose in lysosomes. The encoded preproprotein is proteolytically processed to generate multiple intermediate forms and the mature form of the enzyme. Defects in this gene are the cause of glycogen storage disease II, also known as Pompe's disease, which is an autosomal recessive disorder with a broad clinical spectrum. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, and 3 all encode the same protein.