IKK beta (IKBKB) (NM_001556) Human Untagged Clone

CAT#: SC125322

IKBKB (untagged)-Human inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta (IKBKB), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IKBKB
• Synonyms: IKK-beta; IKK2; IKKB; IMD15; IMD15A; IMD15B; NFKBIKB
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001556 edited
  ATGAGCTGGTCACCTTCCCTGACAACGCAGACATGTGGGGCCTGGGAAATGAAAGAGCGC
  CTTGGGACAGGGGGATTTGGAAATGTCATCCGATGGCACAATCAGGAAACAGGTGAGCAG
  ATTGCCATCAAGCAGTGCCGGCAGGAGCTCAGCCCCCGGAACCGAGAGCGGTGGTGCCTG
  GAGATCCAGATCATGAGAAGGCTGACCCACCCCAATGTGGTGGCTGCCCGAGATGTCCCT
  GAGGGGATGCAGAACTTGGCGCCCAATGACCTGCCCCTGCTGGCCATGGAGTACTGCCAA
  GGAGGAGATCTCCGGAAGTACCTGAACCAGTTTGAGAACTGCTGTGGTCTGCGGGAAGGT
  GCCATCCTCACCTTGCTGAGTGACATTGCCTCTGCGCTTAGATACCTTCATGAAAACAGA
  ATCATCCATCGGGATCTAAAGCCAGAAAACATCGTCCTGCAGCAAGGAGAACAGAGGTTA
  ATACACAAAATTATTGACCTAGGATATGCCAAGGAGCTGGATCAGGGCAGTCTTTGCACA
  TCATTCGTGGGGACCCTGCAGTACCTGGCCCCAGAGCTACTGGAGCAGCAGAAGTACACA
  GTGACCGTCGACTACTGGAGCTTCGGCACCCTGGCCTTTGAGTGCATCACGGGCTTCCGG
  CCCTTCCTCCCCAACTGGCAGCCCGTGCAGTGGCATTCAAAAGTGCGGCAGAAGAGTGAG
  GTGGACATTGTTGTTAGCGAAGACTTGAATGGAACGGTGAAGTTTTCAAGCTCTTTACCC
  TACCCCAATAATCTTAACAGTGTCCTGGCTGAGCGACTGGAGAAGTGGCTGCAACTGATG
  CTGATGTGGCACCCCCGACAGAGGGGCACGGATCCCACGTATGGGCCCAATGGCTGCTTC
  AAGGCCCTGGATGACATCTTAAACTTAAAGCTGGTTCATATCTTGAACATGGTCACGGGC
  ACCATCCACACCTACCCTGTGACAGAGGATGAGAGTCTGCAGAGCTTGAAGGCCAGAATC
  CAACAGGACACGGGCATCCCAGAGGAGGACCAGGAGCTGCTGCAGGAAGCGGGCCTGGCG
  TTGATCCCCGATAAGCCTGCCACTCAGTGTATTTCAGACGGCAAGTTAAATGAGGGCCAC
  ACATTGGACATGGATCTTGTTTTTCTCTTTGACAACAGTAAAATCACCTATGAGACTCAG
  ATCTCCCCACGGCCCCAACCTGAAAGTGTCAGCTGTATCCTTCAAGAGCCCAAGAGGAAT
  CTCGCCTTCTTCCAGCTGAGGAAGGTGTGGGGCCAGGTCTGGCACAGCATCCAGACCCTG
  AAGGAAGATTGCAACCGGCTGCAGCAGGGACAGCGAGCCGCCATGATGAATCTCCTCCGA
  AACAACAGCTGCCTCTCCAAAATGAAGAATTCCATGGCTTCCATGTCTCAGCAGCTCAAG
  GCCAAGTTGGATTTCTTCAAAACCAGCATCCAGATTGACCTGGAGAAGTACAGCGAGCAA
  ACCGAGTTTGGGATCACATCAGATAAACTGCTGCTGGCCTGGAGGGAAATGGAGCAGGCT
  GTGGAGCTCTGTGGGCGGGAGAACGAAGTGAAACTCCTGGTAGAACGGATGATGGCTCTG
  CAGACCGACATTGTGGACTTACAGAGGAGCCCCATGGGCCGGAAGCAGGGGGGAACGCTG
  GACGACCTAGAGGAGCAAGCAAGGGAGCTGTACAGGAGACTAAGGGAAAAACCTCGAGAC
  CAGCGAACTGAGGGTGACAGTCAGGAAATGGTACGGCTGCTGCTTCAGGCAATTCAGAGC
  TTCGAGAAGAAAGTGCGAGTGATCTATACGCAGCTCAGTAAAACTGTGGTTTGCAAGCAG
  AAGGCGCTGGAACTGTTGCCCAAGGTGGAAGAGGTGGTGAGCTTAATGAATGAGGATGAG
  AAGACTGTTGTCCGGCTGCAGGAGAAGCGGCAGAAGGAGCTCTGGAATCTCCTGAAGATT
  GCTTGTAGCAAGGTCCGTGGTCCTGTCAGTGGAAGCCCGGATAGCATGAATGCCTCTCGA
  CTTAGCCAGCCTGGGCAGCTGATGTCTCAGCCCTCCACGGCCTCCAACAGCTTACCTGAG
  CCAGCCAAGAAGAGTGAAGAACTGGTGGCTGAAGCACATAACCTCTGCACCCTGCTAGAA
  AATGCCATACAGGACACTGTGAGGGAACAAGACCAGAGTTTCACGGCCCTAGACTGGAGC
  TGGTTACAGACGGAAGAAGAAGAGCACAGCTGCCTGGAGCAGGCCTCATGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001556 unedited
  GCCGCCCCAGCCCCGCCTTCCCCGCCCCGGGGAGCCCGCCCCCTGCCCCGCGTCCCTGCC
  GACAGAGTTAGCACGACATCAGTATGAGCTGGTCACCTTCCCTGACAACGCAGACATGTG
  GGGCCTGGGAAATGAAAGAGCGCCTTGGGACAGGGGGATTTGGAAATGTCATCCGATGGC
  ACAATCAGGAAACAGGTGAGCAGATTGCCATCAAGCAGTGCCGGCAGGAGCTCAGCCCCC
  GGAACCGAGAGCGGTGGTGCCTGGAGATCCAGATCATGAGAAGGCTGACCCACCCCAATG
  TGGTGGCTGCCCGAGATGTCCCTGAGGGGATGCAGAACTTGGCGCCCAATGACCTGCCCC
  TGCTGGCCATGGAGTACTGCCAAAGAGGAGATCTCCGGAAGTACCTGAACCAGTTTGAGA
  ACTGCTGTGGTCTGCGGGAAAGGTGCATCCTCACCTTGCTGAGAGACATTGCCTCTGCGC
  TTANATACCTTCTTGAAAACAAAATCATCCATCGGGATCTAAAGCCCAAAAACATCTTCC
  TGCAGCAAGGAGAACAAAGGTTAATCCACAAAATTATTGCCCTATGATTTGCCCACGGAG
  CTGGATTAGGGCAGTCTTTTCACATAATTCTGGGGGACCCTGGATTCCTGGCCCCAAACT
  TCTGGAGCCAAAAAGTACCACGGACCGTCGCTATTGGACTTCGGCCCCTGTCTTTGATGG
  ATAAGGGCTTTCGGCCTTTCTTCCCACTGGCAGCCCGGGGTGGTGTTTAAAATCCGGGAA
  AAAAGAGGGGGCTTGTTTGTCCAAACCTAATTGAACGTA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001556 unedited
  NGGCCTAGGGTCTTTATAACGGCCTCCTCATTGGNAAGTTAATACATGTTTCACACACAC
  AATCAGCAGGAGAAAAGTTTCTATAGCCAAATGACATTGATAAACAGTGTTCTTACACAA
  AGTTAAGCCTGTTTCTTTATTATAAGACTTTTCAGATACTTTCCCAGTGCATAGTGTGAA
  TCTCCACAAAGCAAGTGAAACATGAATAGCTCCCTAAACTTACTTGACCACAGAATGCTT
  CATTCTATCTCCATGGAAACCACTTGTGAAAGTCTCCTACTCCAACTTTCTTCTCCAACA
  AAACTCACCTGTTTGTGATCATCTGAACTCATTTTGTGCTCTTGCCTTTATATAAAAGAG
  AAGTTCTGTGAAATGTTGAAGAAGCAGGGTTATGGCACTAGTAATGATCCAGGCACTATA
  CACATTGTTAACATTTTCCATTATTTTAGTTCAGAAGCCCTAACCATATGTATTCTAACA
  GTGGATTGTGGAACCCACTATGAGTGTTTGAGACGTGTCGCCNTATACGGGAGCTATTTT
  GTATATGAATCCATTGATTATCCCTGCTAGTTGCCGTGTGAGACGCCCACTCACCGGGCG
  AGCGCCCTTGCTCGGGCCGGCCTGGGGACCCGGTCGGGCGCCGGCCGGCAGGTTGCTCGG
  CCCTTGCCCGTGCTGGGGGCGGGCTTTGTCTCTTCTCTCCCTAGGTTGCGTGTGGGTCGC
  CCCGCTTGTCGCTGGTGGCGTTTTTCCGTCGGGCGCTTTCGGCGCTTCTTCCTCTNNTAC
  TGCCGCGTGTGGTCGGCACCCCTGCATCTCCCCNCGTTGGTGCTCCCTTTGATGGACTGG
  GCGCGGCGCCGCTCCGATCATTTGTTGCCGTCTTTTCCCGCCCTTTGCCGCTTTTCGTCT
  CCCCCCTCCCCCTTTTTTCCCGCCTTTGTTTTATATAGACGCTTAGCCAGAACAACA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001556
• Insert Size: 4500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001556.1, NP_001547.1
• RefSeq Size: 3916 bp
• RefSeq ORF: 2271 bp
• Locus ID: 3551
• UniProt ID: O14920
• Cytogenetics: 8p11.21
• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, Insulin signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway, Type II diabetes mellitus
• Gene Summary: The protein encoded by this gene phosphorylates the inhibitor in the inhibitor/NF-kappa-B complex, causing dissociation of the inhibitor and activation of NF-kappa-B. The encoded protein itself is found in a complex of proteins. Several transcript variants, some protein-coding and some not, have been found for this gene. [provided by RefSeq, Sep 2011]
  Transcript Variant: This variant (1) encodes the longest isoform (1).