PRMT7 (NM_019023) Human Untagged Clone

CAT#: SC125318

PRMT7 (untagged)-Human protein arginine methyltransferase 7 (PRMT7), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRMT7
• Synonyms: SBIDDS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125318 sequence for NM_019023 edited (data generated by NextGen Sequencing)
  ATGAAGATCTTCTGCAGTCGGGCCAATCCGACCACGGGGTCTGTGGAGTGGCTGGRGGAG
  GATGAACACTATGATTACCACCAGGAGATTGCAAGGTCATCTTATGCAGATATGCTACAT
  GACAAAGACAGAAATGTAAAATACTACCAAGGTATCCGGGCTGCCGTGAGCAGGGTGAAG
  GACAGAGGACAGAAGGCCTTGGTTCTCGACATTGGCACTGGCACGGGACTCTTGTCAATG
  ATGGCGGTCACAGCAGGTGCCGACTTCTGCTATGCCATCGAGGTTTTCAAGCCTATGGCT
  GATGCTGCTGTGAAGATTGTGGAGAAAAATGGCTTTAGTGATAAGATTAAGGTTATCAAC
  AAGCATTCCACCGAGGTGACTGTAGGTCCAGAGGGTGACATGCCATGCCGTGCCAACATC
  CTGGTCACAGAGTTGTTTGACACAGAGCTGATCGGGGAGGGGGCGCTGCCCTCCTATGAG
  CACGCACACAGGCATCTCGTGGAGGAAAATTGTGAGGCCGTGCCCCACAGAGCCACCGTC
  TATGCACAGCTGGTGGAGTCCGGGAGGATGTGGTCGTGGAACAAGCTATTTCCCATCCAC
  GTGCAGACCAGCCTCGGAGAGCAGGTCATCGTCCCTCCCGTTGACGTGGAGAGCTGCCCT
  GGCGCACCCTCTGTCTGTGACATTCAGCTGAACCAGGTGTCACCAGCCGACTTTACAGTC
  CTCAGCGATGTGCTGCCCATGTTCAGCATAGACTTCAGCAAGCAAGTCAGTAGCTCAGCA
  GCCTGCCATAGCAGGCGGTTTGAACCTCTGACATCTGGCCGAGCTCAGGTGGTTCTCTCG
  TGGTGGGACATTGAAATGGACCCTGAGGGGAAGATCAAGTGCACCATGGCCCCCTTCTGG
  GCACACTCAGACCCAGAGGAGATGCAGTGGCGGGACCACTGGATGCAGTGTGTGTACTTC
  CTGCCACAAGAGGAGCCTGTGGTGCAGGGCTCAGCGCTCTATCTGGTAGCCCACCACGAT
  GACTACTGCGTATGGTACAGCCTGCAGAGGACCAGCCCTGAAAAGAATGAGAGAGTCCGC
  CAGATGCGCCCCGTGTGTGACTGCCAGGCTCACCTGCTCTGGAACCGGCCTCGGTTTGGA
  GAGATCAATGACCAGGACAGAACTGATCGATACGTCCAGGCTCTGAGGACCGTGCTGAAG
  CCAGACAGCGTGTGCCTGTGTGTCAGCGATGGCAGCCTGCTCTCCGTGCTGGCCCATCAC
  CTGGGGGTGGAGCAGGTGTTTACAGTCGAGAGTTCAGCAGCTTCTCACAAACTGTTGAGA
  AAAATCTTCAAGGCTAACCACTTGGAAGATAAAATTAACATCATAGAGAAACGGCCGGAA
  TTATTAACAAATGAGGACCTACAGGGCAGAAAGGTCTCTCTCCTCCTGGGCGAGCCGTTC
  TTCACTACCAGCCTGCTGCCGTGGCACAACCTCTACTTCTGGTACGTGCGGACCGCTGTG
  GACCAGCACCTGGGGCCAGGTGCCATGGTGATGCCCCAGGCAGCCTCGCTGCACGCTGTG
  GTTGTGGAGTTCAGGGACCTGTGGCGGATCCGGAGCCCCTGTGGTGACTGCGAAGGCTTC
  GACGTGCACATCATGGACGACATGATTAAGCGTGCCCTGGACTTCAGGGAGAGCAGGGAA
  GCTGAGCCCCACCCGCTGTGGGAGTACCCATGCCGCAGCCTCTCCGAGCCCTGGCAGATC
  CTGACCTTTGACTTCCAGCAGCCGGTGCCCCTGCAGCCCCTGTGTGCCGAGGGCACTGTG
  GAGCTCAGAAGGCCCGGGCAGAGCCACGCAGCGGTGCTATGGATGGAGTACCACCTGACC
  CCGGAGTGCACGCTCAGCACTGGCCTCCTGGAGCCTGCAGACCCCGAGGGGGGCTGCTGC
  TGGAACCCCCACTGCAAGCAGGCCGTCTACTTCTTCAGCCCTGCCCCAGATCCCAGAGCA
  CTGCTGGGTGGCCCACGGACTGTCAGCTATGCAGTGGAGTTTCACCCCGACACAGGCGAC
  ATCATCATGGAGTTCAGGCATGCAGATACCCCAGACTGA
  
  Clone variation with respect to NM_019023.2
  56 a=>r;1797 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_019023 unedited
  GGGCGGTCAGGAATTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGCCCGCC
  TGCTGGCCGCGGTAAAATGGTAGCAGCGGAGGCGAGCGGAGGGTTTCCCGCGGCGGGTGA
  GGCGCTGGATTTCTGACAGTCAGACTTGTCCACAAGAACTCAACTGGCAAGGCTGCTTTT
  CTGTGCTAAAACTGGGGAGCTAGTGGGCACCATGAAGATCTTCTGCAGTCGGGCCAATCC
  GACCACGGGGTCTGTGGAGTGGCTGGAGGAGGATGAACACTATGATTACCACCAGGAGAT
  TGCAAGGTCATCTTATGCAGATATGCTACATGACAAAGACAGAAATGTAAAATACTACCA
  AGGTATCCGGGCTGCCGTGAGCAGGGTGAAGGACAGAGGACAGAAGGCCTTGGTTCTCGA
  CATTGGCACTGGCACGGGACTCTTGTCAATGATGGCGGTCACAGCAGGTGCCGACTTCTG
  CTATGCCATCGAGGTTTTCAAGCCTATGGCTGATGCTGCTGTGAAGATTGTGGAGAAAAA
  TGGCTTTAGTGATAAGATTAAGGTTATCAACAAGCATTCCACCGAGGTGACTGTAGGTCC
  AGAGGGTGACATGCCATGCCGTGCCAACATCCTGGTCACAGAGTTGTTTGACACAACTGG
  ATCGGGGAGGGGGCGCTGCCCTCCTATGAGCACGCACACAGGCATCTCGTGGAGGAAAAT
  TGTGAGGCCGTGCCCCACAGAGCCACCGTCTATGCACAGCTGGNTGGAGTCCGGNAAGAT
  GTNGTTCGTGGAACAAGCTATTTCCATNCACGTGCAGACCAGCCTCGNAGAAGCAGTCAT
  CGTCCCTCCCGTTGACGTGNAGAGCTGCCCTGGCGCACCCTCTGTCTGTGACATTCAGCT
  GAACCCAGTGTCACCAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_019023 unedited
  TTCCTCAGGCCACTTTATGCTCAGNATGGTCAGTCTGGGGTATCTGCATGCCTGAACTCC
  ATGATGATGTCGCCTGTGTCGGGGTGAAACTCCACTGCATAGCTGACAGTCCGTGGGCCA
  CCCAGCAGTGCTCTGGGATCTGGGGCAGGGCTGAAGAAGTAGACGGCCTGCTTGCAGTGG
  GGGTTCCAGCAGCAGCCCCCCTCGGGGTCTGCAGGCTCCAGGAGGCCAGTGCTGAGCGTG
  CACTCCGGGGTCAGGTGGTACTCCATCCATAGCACCGCTGCGTGGCTCTGCCCGGGCCTT
  CTGAGCTCCACAGTGCCCTCGGCACACAGGGGCTGCAGGGGCACCGGCTGCTGGAAGTCA
  AAGGTCAGGATCTGCCAGGGCTCGGAGAGGCTGCGGCATGGGTACTCCCACAGCGGGTGG
  GGCTCAGCTTCCCTGCTCTCCCTGAAGTCCAGGGCACGCTTAATCATGTCGTCCATGATG
  TGCACGTCGAAGCCTTCGCAGTCACCACAGGGGCTCCGGATCCGCCACAGGTCCCTGAAC
  TCCACAACCACAGCGTGCAGCGAGGCTGCCTGGGGCATCACCATGGCACCTGGCCNCAGG
  TGCTGGTCCACAGCGGTCCGCACGTACCAGAAGTAGAGGTTGTGCCACGGCAGCAGGCTG
  GTAGTGAAGAACGGCTCGCCCAGNANGAGAGAGACCCTTCTGCCCTGTANGTCCTCATTT
  GTTAATAATTCCCGCCGTTTCTCTATGATGGTAATTTNTATCTCCAAGTGGTTAGCCTTG
  AAAGATTTTCTCACAGTTTGTGAGGAGCTGCTGAACTCTCGACTGTAAACACCCTGCTCA
  CCCCCAGGTGATGGCCAGCACCGAGAGCAGGCTGCATCGCTGACCCCAAGCCCACGCTGG
  CTGGGTTCAGAACGGTCTCAGACCTGGACTTTCGATCAGTTCTGTCTGTCAA
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_019023
• Insert Size: 2500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019023.1, NP_061896.1
• RefSeq Size: 2390 bp
• RefSeq ORF: 2079 bp
• Locus ID: 54496
• UniProt ID: Q9NVM4
• Cytogenetics: 16q22.1
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the protein arginine N-methyltransferase family of proteins. The encoded enzyme transfers single methyl groups to arginine residues to generate monomethylarginines on histone proteins as well as other protein substrates. This enzyme plays a role in a wide range of biological processes, including neuronal differentiation, male germ line imprinting, small nuclear ribonucleoprotein biogenesis, and regulation of the Wnt signaling pathway. Mutations in this gene underlie multiple related syndromes in human patients characterized by intellectual disability, short stature and other features. The encoded protein may promote breast cancer cell invasion and metastasis in human patients. [provided by RefSeq, May 2017]
  Transcript Variant: This variant (1) includes a 3' terminal exon that extends past a splice site that is used in variant 4, which results in a novel 3' coding region and 3' UTR compared to variant 4. The encoded isoform (1) is shorter and has a distinct C-terminus compared to isoform 4. Both variants 1 and 3 encode the same isoform (1).