Ephrin A1 (EFNA1) (NM_004428) Human Untagged Clone

SKU SC125307

EFNA1 (untagged)-Human ephrin-A1 (EFNA1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: Ephrin A1
• Synonyms: B61; ECKLG; EFL1; EPLG1; GMAN; LERK-1; LERK1; TNFAIP4
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125307 sequence for NM_004428 edited (data generated by NextGen Sequencing); ATGGAGTTCCTCTGGGCCCCTCTCTTGGGTCTGTGCTGCAGTCTGGCCGCTGCTGATCGC; CACACCGTCTTCTGGAACAGTTCAAATCCCAAGTTCCGGAATGAGGACTACACCATACAT; GTGCAGCTGAATGACTACGTGGACATCATCTGTCCGCACTATGAAGATCACTCTGTGGCA; GACGCTGCCATGGAGCAGTACATACTGTATCTGGTGGAGCATGAGGAGTACCAGCTGTGC; CAGCCCCAGTCCAAGGACCAAGTCCGCTGGCAGTGCAACCGGCCCAGTGCCAAGCATGGC; CCGGAGAAGCTGTCTGAGAAGTTCCAGCGCTTCACACCTTTCACCCTGGGCAAGGAGTTC; AAAGAAGGACACAGCTACTACTACATCTCCAAACCCATCCACCAGCATGAAGACCGCTGC; TTGAGGTTGAAGGTGACTGTCAGTGGCAAAATCACTCACAGTCCTCAGGCCCATGACAAT; CCACAGGAGAAGAGACTTGCAGCAGATGACCCAGAGGTGCGGGTTCTACATAGCATCGGT; CACAGTGCTGCCCCACGCCTCTTCCCACTTGCCTGGACTGTGCTGCTCCTTCCACTTCTG; CTGCTGCAAACCCCGTGA; ; Clone variation with respect to NM_004428.2; 210 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004428 unedited; TCTATCACCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAA; GCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG; CGAATTCGGCACGAGGGCTGACTGCGCCGCGGAGAAAGCCAGTGGGAACCCAGACCCATA; GGAGACCCGCGTTCCCGCTCGGCCTGGCCAGGCCCCGCGCTATGGAGTTCCTCTGGGCCC; CTCTCTTGGGTCTGTGCTGCAGTCTGGCCGCTGCTGATCGCCACACCGTCTTCTGGAACA; GTTCAAATCCCAAGTTCCGGAATGAGGACTACACCATACATGTGCAGCTGAATGACTACG; TGGACATCATCTGTCCGCACTATGAAGATCACTCTGTGGCAGACGCTGCCATGGAGCAGT; ACATACTGTATCTGGTGGAGCATGAGGAGTACCAGCTGTGCCAGCCCCAGTCCAAGGACC; AAGTCCGCTGGCAGTGCAACCGGCCCAGTGCCAAGCATGGCCCGGAGAAGCTGTCTGAGA; AGTTTCAGCGCTTCACACCTTTCACCCTGGGCAAGGAGTTCANAGAAGGACACAGCTACT; ACTACATCTCCAAACCCATCCACCAGCATGAAGACCGCTGCTTGAGGTTGAAGGTGACTG; TCAGTGGCAAAATCACTCACAGTCCTCAGGCCCATGACAATCCACAGGAGAAGAGACTTG; CAGCAGATGACCCAGAGGTGCGGGTTCTACATAGCATCGGTCACAGTGCTGCCCCACGCT; CTTCCCACTTTGCTGAACTGTGCTGCTCCTTTCACTTCTNTGCTGCGAACCCCGTGGAGG; TGTGTTGCCACACCTGGCTTTATGAGGGACAGGCTTGANAAGGGAACAGGCCTCCAAACT; GGTCTGGGGCCATTTCAAAGCCCCCAGCCTGGGAACAATCCCC;
• Restriction Sites: ECoRI-NOT
• ACCN: NM_004428
• Insert Size: 800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004428.2, NP_004419.2
• RefSeq Size: 1590 bp
• RefSeq ORF: 618 bp
• Locus ID: 1942
• UniProt ID: P20827
• Cytogenetics: 1q22
• Domains: Ephrin
• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance
• Summary: This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNA class ephrin which binds to the EPHA2, EPHA4, EPHA5, EPHA6, and EPHA7 receptors. Two transcript variants that encode different isoforms were identified through sequence analysis. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) encodes the longer isoform (a).