ADAMTS4 (NM_005099) Human Untagged Clone

CAT#: SC125261

ADAMTS4 (untagged)-Human ADAM metallopeptidase with thrombospondin type 1 motif, 4 (ADAMTS4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ADAMTS4
• Synonyms: ADAMTS-2; ADAMTS-4; ADMP-1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005099 edited
  GGGAACCCACAGGGAGACCCACAGACACATATGCACGAGAGAGACAGAGGAGGAAAGAGA
  CAGAGACAAAGGCACAGCGGAAGAAGGCAGAGACAGGGCAGGCACAGAAGCGGCCCAGAC
  AGAGTCCTACAGAGGGAGAGGCCAGAGAAGCTGCAGAAGACACAGGCAGGGAGAGACAAA
  GATCCAGGAAAGGAGGGCTCAGGAGGAGAGTTTGGAGAAGCCAGACCCCTGGGCACCTCT
  CCCAAGCCCAAGGACTAAGTTTTCTCCATTTCCTTTAACGGTCCTCAGCCCTTCTGAAAA
  CTTTGCCTCTGACCTTGGCAGGAGTCCAAGCCCCCAGGCTACAGAGAGGAGCTTTCCAAA
  GCTAGGGTGTGGAGGACTTGGTGCCCTAGACGGCCTCAGTCCCTCCCAGCTGCAGTACCA
  GTGCCATGTCCCAGATAGGCTCGCATCCCGGGAGGGGCTTGGCAGGGCGCTGGCTGTGGG
  GAGCCCAACCCTGCCTCCTGCTCCCCATTGTGCCGCTCTCCTGGCTGGTGTGGCTGCTTC
  TGCTACTGCTGGCCTCTCTCCTGCCCTCAGCCCGGCTGGCCAGCCCCCTCCCCCGGGAGG
  AGGAGATCGTGTTTCCAGAGAAGCTCAACGGCAGCGTCCTGCCTGGCTCGGGCGCCCCTG
  CCAGGCTGTTGTGCCGCTTGCAGGCCTTTGGGGAGACGCTGCTACTAGAGCTGGAGCAGG
  ACTCCGGTGTGCAGGTCGAGGGGCTGACAGTGCAGTACCTGGGCCAGGCGCCTGAGCTGC
  TGGGTGGAGCAGAGCCTGGCACCTACCTGACTGGCACCATCAATGGAGATCCGGAGTCGG
  TGGCATCTCTGCACTGGGATGGGGGAGCCCTGTTAGGCGTGTTACAATATCGGGGGGCTG
  AACTCCACCTCCAGCCCCTGGAGGGAGGCACCCCTAACTCTGCTGGGGGACCTGGGGCTC
  ACATCCTACGCCGGAAGAGTCCTGCCAGCGGTCAAGGTCCCATGTGCAACGTCAAGGCTC
  CTCTTGGAAGCCCCAGCCCCAGACCCCGAAGAGCCAAGCGCTTTGCTTCACTGAGTAGAT
  TTGTGGAGACACTGGTGGTGGCAGATGACAAGATGGCCGCATTCCACGGTGCGGGGCTAA
  AGCGCTACCTGCTAACAGTGATGGCAGCAGCAGCCAAGGCCTTCAAGCACCCAAGCATCC
  GCAATCCTGTCAGCTTGGTGGTGACTCGGCTAGTGATCCTGGGGTCGGGCGAGGAGGGGC
  CCCAAGTGGGGCCCAGTGCTGCCCAGACCCTGCGCAGCTTCTGTGCCTGGCAGCGGGGCC
  TCAACACCCCTGAGAACTCGGACCCTGACCACTTTGACACAGCCATTCTGTTTACCCGTC
  AGGACCTGTGTGGAGTCTCCACTTGCGACACGCTGGGTATGGCTGATGTGGGCACCGTCT
  GTGACCCGGCTCGGAGCTGTGCCATTGTGGAGGATGATGGGCTCCAGTCAGCCTTCACTG
  CTGCTCATGAACTGGGTCATGTCTTCAACGTGCTCCATGACAACTCCAAGCCATGCATCA
  GTTTGAATGGGCCTTTGAGCACCTCTCGCCATGTCATGGCCCCTGTGATGGCTCATGTGG
  ATCCTGAGGAGCCCTGGTCCCCCTGCAGTGCCCGCTTCATCACTGACTTCCTGGACAATG
  GCTATGGGCACTGTCTCTTAGACAAACCAGAGGCTCCATTGCATCTGCCTGTGACTTTCC
  CTGGCAAGGACTATGATGCTGACCGCCAGTGCCAGCTGACCTTCGGGCCCGACTCACGCC
  ATTGTCCACAGCTGCCGCCGCCCTGTGCTGCCCTCTGGTGCTCTGGCCACCTCAATGGCC
  ATGCCATGTGCCAGACCAAACACTCGCCCTGGGCCGATGGCACACCCTGCGGGCCCGCAC
  AGGCCTGCATGGGTGGTCGCTGCCTCCACATGGACCAGCTCCAGGACTTCAATATTCCAC
  AGGCTGGTGGCTGGGGTCCTTGGGGACCATGGGGTGACTGCTCTCGGACCTGTGGGGGTG
  GTGTCCAGTTCTCCTCCCGAGACTGCACGAGGCCTGTCACCCGGAATGGTGGCAAGTACT
  GTGAGGGCCGCCGTGCCCGCTTCCGCTCCTGCAACACTGAGGACTGCCCAACTGGCTCAG
  CCCTGACCTTCCGCGAGGAGCAGTGTGCTGCCTACAACCACCGCACCGACCTCTTCAAGA
  GCTTCCCAGGGCCCATGGACTGGGTTCCTCGCTACACAGGCGTGGCCCCCCAGGACCAGT
  GCAAACTCACCTGCCAGGCCCAGGCACTGGGCTACTACTATGTGCTGGAGCCACGGGTGG
  TAGATGGGACCCCCTGTTCCCCGGACAGCTCCTCGGTCTGTGTCCAGGGCCGATGCATCC
  ATGCTGGCTGTGATCGCATCATTGGCTCCAAGAAGAAGTTTGACAAGTGCATGGTGTGCG
  GAGGGGACGGTTCTGGTTGCAGCAAGCAGTCAGGCTCCTTCAGGAAATTCAGGTACGGAT
  ACAACAATGTGGTCACTATCCCCGCGGGGGCCACCCACATTCTTGTCCGGCAGCAGGGAA
  ACCCTGGCCACCGGAGCATCTACTTGGCCCTGAAGCTGCCAGATGGCTCCTATGCCCTCA
  ATGGTGAATACACGCTGATGCCCTCCCCCACAGATGTGGTACTGCCTGGGGCAGTCAGCT
  TGCGCTACAGCGGGGCCACTGCAGCCTCAGAGACACTGTCAGGCCATGGGCCACTGGCCC
  AGCCTTTGACACTGCAAGTCCTAGTGGCTGGCAACCCCCAGGACACACGCCTCCGATACA
  GCTTCTTCGTGCCCCGGCCGACCCCTTCAACGCCACGCCCCACTCCCCAGGACTGGCTGC
  ACCGAAGAGCACAGATTCTGGAGATCCTTCGGCGGCGCCCCTGGGCGGGCAGGAAATAAC
  CTCACTATCCCGGCTGCCCTTTCTGGGCACCGGGGCCTCGGACTTAGCTGGGAGAAAGAG
  AGAGCTTCTGTTGCTGCCTCATGCTAAGACTCAGTGGGGAGGGGCTGTGGGCGTGAGACC
  TGCCCCTCCTCTCTGCCCTAATGCGCAGGCTGGCCCTGCCCTGGTTTCCTGCCCTGGGAG
  GCAGTGATGGGTTAGTGGATGGAAGGGGCTGACAGACAGCCCTCCATCTAAACTGCCCCC
  TCTGCCCTGCGGGTCACAGGAGGGAGGGGGAAGGCAGGGAGGGCCTGGGCCCCAGTTGTA
  TTTATTTAGTATTTATTCACTTTTATTTAGCACCAGGGAAGGGGACAAGGACTAGGGTCC
  TGGGGAACCTGACCCCTGACCCCTCATAGCCCTCACCGTGGGGCTAGGAAATCCAGGGTG
  GTGGTGATAGGTATAAGTGGTGTGTGTATGCGTGTGTGTGTGTGTGAAAATGTGTGTGTG
  CTTATGTATGAGGTACAACCTGTTCTGCTTTCCTCTTCCTGAATTTTATTTTTTGGGAAA
  AGAAAAGTCAAGGGTAGGGTGGGCCTTCAGGGAGTGAGGGATTATCTTTTTTTTTTTTTT
  CTTTCTTTCTTTCTTTTTTTTTTTTGAGACAGAATCTCGCTCTGTCGCCCAGGCTGGAGT
  GCAATGGCACAATCTCGGCTCACTGCATCCTCCGCCTCCCGGGTTCAAGTGATTCTCATG
  CCTCAGCCTCCTGAGTAGCTGGGATTACAGGCTCCTGCCACCACGCCCGGCTAATTTTTG
  TTTTGTTTTGTTTGGAGACAGAGTCTCGCTATTGTCACCAGGGCTGGAATGATTTCAGCT
  CACTGCAACCTTCGCCACCTGGGTTCCAGCAATTCTCCTGCCTCAGCCTCCCGAGTAGCT
  GAGATTATAGGCACCTACCACCACGCCCGGCTAATTTTTGTATTTTTAGTAGAGACGGGG
  TTTCACCATGTTGGCCAGGCTGGTCTCGAACTCCTGACCTTAGGTGATCCACTCGCCTTC
  ATCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCGTGCCTGGCCACGCCCAACTAAT
  TTTTGTATTTTTAGTAGAGACAGGGTTTCACCATGTTGGCCAGGCTGCTCTTGAACTCCT
  GACCTCAGGTAATCGACCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCA
  CCACGCCCGGTACATATTTTTTAAATTGAATTCTACTATTTATGTGATCCTTTTGGAGTC
  AGACAGATGTGGTTGCATCCTAACTCCATGTCTCTGAGCATTAGATTTCTCATTTGCCAA
  TAATAATACCTCCCTTAGAAGTTTGTTGTGAGGATTAAATAATGTAAATAAAGAACTAGC
  ATAACAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_005099
• Insert Size: 4400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to contain 4 SNPs compared with NM_005099.3.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005099.3, NP_005090.2
• RefSeq Size: 4342 bp
• RefSeq ORF: 2514 bp
• Locus ID: 9507
• UniProt ID: O75173
• Cytogenetics: 1q23.3
• Protein Families: Druggable Genome, Protease, Secreted Protein, Transmembrane
• Gene Summary: This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of this family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene lacks a C-terminal TS motif. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The expression of this gene is upregulated in arthritic disease and this may contribute to disease progression through the degradation of aggrecan. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the shorter isoform (1).