GLUT4 (SLC2A4) (NM_001042) Human Untagged Clone

SKU SC125240

SLC2A4 (untagged)-Human solute carrier family 2 (facilitated glucose transporter), member 4 (SLC2A4)

Product Data

• Type: Human Untagged Clone
• Target Symbol: GLUT4
• Synonyms: GLUT4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125240 sequence for NM_001042 edited (data generated by NextGen Sequencing); ATGCCGTCGGGCTTCCAACAGATAGGCTCCGAAGATGGGGAACCCCCTCAGCAGCGAGTG; ACTGGGACCCTGGTCCTTGCTGTGTTCTCTGCGGTGCTTGGCTCCCTGCAGTTTGGGTAC; AACATTGGGGTCATCAATGCCCCTCAGAAGGTGATTGAACAGAGCTACAATGAGACGTGG; CTGGGGAGGCAGGGGCCTGAGGGACCCAGCTCCATCCCTCCAGGCACCCTCACCACCCTC; TGGGCCCTCTCCGTGGCCATCTTTTCCGTGGGCGGCATGATTTCCTCCTTCCTCATTGGT; ATCATCTCTCAGTGGCTTGGAAGGAAAAGGGCCATGCTGGTCAACAATGTCCTGGCGGTG; CTGGGGGGCAGCCTCATGGGCCTGGCCAATGCTGCTGCCTCCTATGAAATGCTCATCCTT; GGACGATTCCTCATTGGCGCCTACTCAGGGCTGACATCAGGGCTGGTGCCCATGTACGTG; GGGGAGATTGCTCCCACTCACCTGCGGGGCGCCCTGGGGACGCTCAACCAACTGGCCATT; GTTATCGGCATTCTGATCGCCCAGGTGCTGGGCTTGGAGTCCCTCCTGGGCACTGCCAGC; CTGTGGCCACTGCTCCTGGGCCTCACAGTGCTACCTGCCCTCCTGCAGCTGGTCCTGCTG; CCCTTCTGTCCCGAGAGCCCCCGCTACCTCTACATCATCCAGAATCTCGAGGGGCCTGCC; AGAAAGAGTCTGAAGCGCCTGACAGGCTGGGCCGATGTTTCTGGAGTGCTGGCTGAGCTG; AAGGATGAGAAGCGGAAGCTGGAGCGTGAGCGGCCACTGTCCCTGCTCCAGCTCCTGGGC; AGCCGTACCCACCGGCAGCCCCTGATCATTGCGGTCGTGCTGCAGCTGAGCCAGCAGCTC; TCTGGCATCAATGCTGTTTTCTATTATTCGACCAGCATCTTCGAGACAGCAGGGGTAGGC; CAGCCTGCCTATGCCACCATAGGAGCTGGTGTGGTCAACACAGTCTTCACCTTGGTCTCG; GTGTTGTTGGTGGAGCGGGCGGGGCGCCGGACGCTCCATCTCCTGGGCCTGGCGGGCATG; TGTGGCTGTGCCATCCTGATGACTGTGGCTCTGCTCCTGCTGGAGCGAGTTCCAGCCATG; AGCTACGTCTCCATTGTGGCCATCTTTGGCTTCGTGGCATTTTTTGAGATTGGCCCTGGC; CCCATTCCTTGGTTCATCGTGGCCGAGCTCTTCAGCCAGGGACCCCGCCCGGCAGCCATG; GCTGTGGCTGGTTTCTCCAACTGGACGAGCAACTTCATCATTGGCATGGGTTTCCAGTAT; GTTGCGGAGGCTATGGGGCCCTACGTCTTCCTTCTATTTGCGGTCCTCCTGCTGGGCTTC; TTCATCTTCACCTTCTTAAGAGTACCTGAAACTCGAGGCCGGACGTTTGACCAGATCTCA; GCTGCCTTCCACCGGACACCCTCTCTTTTAGAGCAGGAGGTGAAACCCAGCACAGAACTT; GAGTATTTAGGGCCAGATGAGAACGACTGA; ; Clone variation with respect to NM_001042.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001042 unedited; GGGGAAGGAGNNNTTTAGTCGATTTGTATACGAAGGNNTTANGGCGGCCGCGAATTCGGC; ACGAGACCAGATCCGCGGGAGCCCCACTGCTCTCCGGGTCCTTGGCTTGTGGCTGTGGGT; CCCATCGGGCCCGCCCTCGCACGTCACTCCGGGACCCCCGCGGCCTCCGCAGGTTCTGCG; CTCCAGGCCGGAGTCAGAGACTCCAGGATCGGTTCTTTCATCTTCGCCGCCCCTGCGCGT; CCAGCTCTTCTAAGACGAGATGCCGTCGGGCTTCCAACAGATAGGCTCCGAAGATGGGGA; ACCCCCTCAGCAGCGAGTGACTGGGACCCTGGTCCTTGCTGTGTTCTCTGCGGTGCTTGG; CTCCCTGCAGTTTGGGTACAACATTGGGGTCATCAATGCCCCTCAGAAGGTGATTGAACA; GAGCTACAATGAGACGTGGCTGGGGAGGCAGGGGCCTGAGGGACCCAGCTCCATCCCTCC; AGGCACCCTCACCACCCTCTGGGCCCTCTCCGTGGCCATCTTTTCCGTGGGCGGCATGAT; TTCCTCCTTCCTCATTGGTATCATCTCTCAGTGGCTTGGAAGGAAAAGGGCCATGCTGGT; CAACAATGTCCTTGCGGTGCTGGGGGCAGCCTCATGGGCCTGGCCATGCTGCTGCTCTAT; GAATGCTATCNTGACGATCTCATGCGCTATCGGCTGCTCAGCTGTCCATGTCTGGGAATG; TCATACTGCGGGCCTGGACTACACTGCTGTTGC;
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_001042 unedited; TGGCATGGGTTTCAGTATGTTGCGGAGGCTATGGGGCCCTACGTCTTCCTTCTATTTGCG; GTCCTCCTGCTGGGCTTCTTCATCTTCACCTTCTTAAGAGTACCTGAAACTCGAGGCCGG; ACGTTTGACCAGATCTCAGCTGCCTTCCACCGGACACCCTCTCTTTTAGAGCAGGAGGTG; AAACCCAGCACAGAACTTGAGTATTTAGGGCCAGATGAGAACGACTGAGGGGCCAGGCAG; GGGTGGGAGAGCCAGCTCTCTCTACCCGGCCCAGAGACCCCTTCCTTTCCTCTGCAGCAC; TTTAACCCTCTCTTCCCTATTATTTCCGGGTGGAAAAGAATCCCTGCAGCCTGGTAGAAT; TGGGAAGCTGGGGGAAGGGTGGTCTGAGCACCCCCTCATTCCCCTCGTGTGACTCTCTTG; GATTATTTATGTGTTGTGGTTTGGCCGTGGCCATCAGGGTGGGCCACTCTCCCCTCCCTC; TTCCTTCCCCCATCCCCTTTCCTCCCCACCTTCCCCAGACTCAGCTCCAGAATACCTTCT; TCGCTGCTAGAGAAGGGGGATTGGAGGGAAGACAGGTCTAGACTTTCTCAGTGGGACAAA; CCAGAGCAGAGAGCAGGACAGGAGACAAGAAATCCAGTTTCCCACCACCTTGGACTCCTC; CCACAATCTGGGACTTTCACTGAATTCTTGCCACGCAGACTCTGGGCAAAGGGGTTTTTT; TTTTTTTTTTTTTTTTTTTTTTTGAGACAGTCTCGCTCTGTCGCCCAGGCTCGAGTGCAG; TGGCGTGATCTTG;
• Restriction Sites: Please inquire
• ACCN: NM_001042
• Insert Size: 3000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001042.1, NP_001033.1
• RefSeq Size: 2128 bp
• RefSeq ORF: 1530 bp
• Locus ID: 6517
• UniProt ID: P14672
• Cytogenetics: 17p13.1
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, Insulin signaling pathway, Type II diabetes mellitus
• Summary: This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM). [provided by RefSeq, Jul 2008]