CD247 (NM_198053) Human Untagged Clone

CAT#: SC125046

CD247 (untagged)-Human CD247 molecule (CD247), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD247
• Synonyms: CD3-ZETA; CD3H; CD3Q; CD3Z; IMD25; T3Z; TCRZ
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125046 sequence for NM_198053 edited (data generated by NextGen Sequencing)
  ATGAAGTGGAAGGCGCTTTTCACCGCGGCCATCCTGCAGGCACAGTTGCCGATTACAGAG
  GCACAGAGCTTTGGCCTGCTGGATCCCAAACTCTGCTACCTGCTGGATGGAATCCTCTTC
  ATCTATGGTGTCATTCTCACTGCCTTGTTCCTGAGAGTGAAGTTCAGCAGGAGCGCAGAC
  GCCCCCGCGTACCAGCAGGGCCAGAACCAGCTCTATAACGAGCTCAATCTAGGACGAAGA
  GAGGAGTACGATGTTTTGGACAAGAGACGTGGCCGGGACCCTGAGATGGGGGGAAAGCCG
  NNGAGAAGGAAGAACCCTCAGGAAGGCCTGTACAATGAACTGCAGAAAGATAAGATGGCG
  GAGGCCTACAGTGAGATTGGGATGAAAGGCGAGCGCCGGAGGGGCAAGGGGCACGATGGC
  CTTTACCAGGGTCTCAGTACAGCCACCAAGGACACCTACGACGCCCTTCACATGCAGGCC
  CTGCCCCCTCGCTAA
  
  Clone variation with respect to NM_198053.2
  301 c=>n;302 a=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_198053 unedited
  NNGGTTCAAATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGCAGAGAC
  TCCTTTTCTCCTAACCGTCCCGGCCACCGCTGCCTCAGCCTCTGCCTCCCAGCCTCTTTC
  TGAGGGAAAGGACAAGATGAAGTGGAAGGCGCTTTTCACCGCGGCCATCCTGCAGGCACA
  GTTGCCGATTACAGAGGCACAGAGCTTTGGCCTGCTGGATCCCAAACTCTGCTACCTGCT
  GGATGGAATCCTCTTCATCTATGGTGTCATTCTCACTGCCTTGTTCCTGAGAGTGAAGTT
  CAGCAGGAGCGCAGACGCCCCCGCGTACCAGCAGGGCCAGAACCAGCTCTATAACGAGCT
  CAATCTAGGACGAAGAGAGGAGTACGATGTTTTGGACAAGAGACGTGGCCGGGACCCTGA
  GATGGGGGGAAAGCCGAGAAGGAAGAACCCTCAGGAAGGCCTGTACAATGAACTGCAGAA
  AGATAAGATGGCGGAGGCCTACAGTGAGATTGGGATGAAAGGCGAGCGCCGGAGGGGCAA
  GGGGCACGATGGCCTTTACCAGGGTCTCAGTACAGCCACCAAGGACACCTACGACGCCCT
  TCACATGCAGGCCCTGCCCCCTCGCTAACAGCCAGGGGATTTCACCACTCAAAGGCCAGA
  CCTGCAGACGCCCAGATTATGAGACACAGGATGAAGCATTTACCAACCCGGTTCACTCTT
  CTCAGCACTGAAGTATTCCNCTTTATGTACAGGGATGCTTTGGNTATATTTAGCTNCCAA
  CCTTCCACACAGACTGGTGTCCCTGCACTCTTAANGGGAGTGTACTCCCAGGGCTTACGG
  NCCTGGNCTTGGCCCTCTGNNTNTGCCGTGGTGCANGTAAACTGCTCCCGTGCGTTTCTC
  GTCTCCCTGGGGAGGCGGCGCCTGCTCTCACCTGGNTGTGGAATCTCGTTTTGAAGCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_198053
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198053.1, NP_932170.1
• RefSeq Size: 1677 bp
• RefSeq ORF: 495 bp
• Locus ID: 919
• UniProt ID: P20963
• Cytogenetics: 1q24.2
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway
• Gene Summary: The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longer isoform (1).