CD247 (NM_198053) Human Untagged Clone

SKU SC125046

CD247 (untagged)-Human CD247 molecule (CD247), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: CD247
• Synonyms: CD3-ZETA; CD3H; CD3Q; CD3Z; IMD25; T3Z; TCRZ
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125046 sequence for NM_198053 edited (data generated by NextGen Sequencing); ATGAAGTGGAAGGCGCTTTTCACCGCGGCCATCCTGCAGGCACAGTTGCCGATTACAGAG; GCACAGAGCTTTGGCCTGCTGGATCCCAAACTCTGCTACCTGCTGGATGGAATCCTCTTC; ATCTATGGTGTCATTCTCACTGCCTTGTTCCTGAGAGTGAAGTTCAGCAGGAGCGCAGAC; GCCCCCGCGTACCAGCAGGGCCAGAACCAGCTCTATAACGAGCTCAATCTAGGACGAAGA; GAGGAGTACGATGTTTTGGACAAGAGACGTGGCCGGGACCCTGAGATGGGGGGAAAGCCG; NNGAGAAGGAAGAACCCTCAGGAAGGCCTGTACAATGAACTGCAGAAAGATAAGATGGCG; GAGGCCTACAGTGAGATTGGGATGAAAGGCGAGCGCCGGAGGGGCAAGGGGCACGATGGC; CTTTACCAGGGTCTCAGTACAGCCACCAAGGACACCTACGACGCCCTTCACATGCAGGCC; CTGCCCCCTCGCTAA; ; Clone variation with respect to NM_198053.2; 301 c=>n;302 a=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_198053 unedited; NNGGTTCAAATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGCAGAGAC; TCCTTTTCTCCTAACCGTCCCGGCCACCGCTGCCTCAGCCTCTGCCTCCCAGCCTCTTTC; TGAGGGAAAGGACAAGATGAAGTGGAAGGCGCTTTTCACCGCGGCCATCCTGCAGGCACA; GTTGCCGATTACAGAGGCACAGAGCTTTGGCCTGCTGGATCCCAAACTCTGCTACCTGCT; GGATGGAATCCTCTTCATCTATGGTGTCATTCTCACTGCCTTGTTCCTGAGAGTGAAGTT; CAGCAGGAGCGCAGACGCCCCCGCGTACCAGCAGGGCCAGAACCAGCTCTATAACGAGCT; CAATCTAGGACGAAGAGAGGAGTACGATGTTTTGGACAAGAGACGTGGCCGGGACCCTGA; GATGGGGGGAAAGCCGAGAAGGAAGAACCCTCAGGAAGGCCTGTACAATGAACTGCAGAA; AGATAAGATGGCGGAGGCCTACAGTGAGATTGGGATGAAAGGCGAGCGCCGGAGGGGCAA; GGGGCACGATGGCCTTTACCAGGGTCTCAGTACAGCCACCAAGGACACCTACGACGCCCT; TCACATGCAGGCCCTGCCCCCTCGCTAACAGCCAGGGGATTTCACCACTCAAAGGCCAGA; CCTGCAGACGCCCAGATTATGAGACACAGGATGAAGCATTTACCAACCCGGTTCACTCTT; CTCAGCACTGAAGTATTCCNCTTTATGTACAGGGATGCTTTGGNTATATTTAGCTNCCAA; CCTTCCACACAGACTGGTGTCCCTGCACTCTTAANGGGAGTGTACTCCCAGGGCTTACGG; NCCTGGNCTTGGCCCTCTGNNTNTGCCGTGGTGCANGTAAACTGCTCCCGTGCGTTTCTC; GTCTCCCTGGGGAGGCGGCGCCTGCTCTCACCTGGNTGTGGAATCTCGTTTTGAAGCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_198053
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_198053.1, NP_932170.1
• RefSeq Size: 1677 bp
• RefSeq ORF: 495 bp
• Locus ID: 919
• UniProt ID: P20963
• Cytogenetics: 1q24.2
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway
• Summary: The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) encodes the longer isoform (1).