PYK2 (PTK2B) (NM_173174) Human Untagged Clone

CAT#: SC124895

PTK2B (untagged)-Human PTK2B protein tyrosine kinase 2 beta (PTK2B), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PYK2
• Synonyms: CADTK; CAKB; FADK2; FAK2; PKB; PTK; PYK2; RAFTK
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_173174, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCTGGGGTGTCCGAGCCCCTGAGTCGAGTAAAGTTGGGCACGTTACGCCGGCCTGAAGGCCCTGCAG
  AGCCCATGGTGGTGGTACCAGTAGATGTGGAAAAGGAGGACGTGCGTATCCTCAAGGTCTGCTTCTATAG
  CAACAGCTTCAATCCTGGGAAAAACTTCAAACTGGTCAAATGCACTGTCCAGACGGAGATCCGGGAGATC
  ATCACCTCCATCCTGCTGAGCGGGCGGATCGGGCCCAACATCCGGTTGGCTGAGTGCTATGGGCTGAGGC
  TGAAGCACATGAAGTCCGATGAGATCCACTGGCTGCACCCACAGATGACGGTGGGTGAGGTGCAGGACAA
  GTATGAGTGTCTGCACGTGGAAGCCGAGTGGAGGTATGACCTTCAAATCCGCTACTTGCCAGAAGACTTC
  ATGGAGAGCCTGAAGGAGGACAGGACCACGCTGCTCTATTTTTACCAACAGCTCCGGAACGACTACATGC
  AGCGCTACGCCAGCAAGGTCAGCGAGGGCATGGCCCTGCAGCTGGGCTGCCTGGAGCTCAGGCGGTTCTT
  CAAGGATATGCCCCACAATGCACTTGACAAGAAGTCCAACTTCGAGCTCCTAGAAAAGGAAGTGGGGCTG
  GACTTGTTTTTCCCAAAGCAGATGCAGGAGAACTTAAAGCCCAAACAGTTCCGGAAGATGATCCAGCAGA
  CCTTCCAGCAGTACGCCTCGCTCAGGGAGGAGGAGTGCGTCATGAAGTTCTTCAACACTCTCGCCGGCTT
  CGCCAACATCGACCAGGAGACCTACCGCTGTGAACTCATTCAAGGATGGAACATTACTGTGGACCTGGTC
  ATTGGCCCTAAAGGGATCCGCCAGCTGACTAGTCAGGACGCAAAGCCCACCTGCCTGGCCGAGTTCAAGC
  AGATCAGGTCCATCAGGTGCCTCCCGCTGGAGGAGGGCCAGGCAGTACTTCAGCTGGGCATTGAAGGTGC
  CCCCCAGGCCTTGTCCATCAAAACCTCATCCCTAGCAGAGGCTGAGAACATGGCTGACCTCATAGACGGC
  TACTGCCGGCTGCAGGGTGAGCACCAAGGCTCTCTCATCATCCATCCTAGGAAAGATGGTGAGAAGCGGA
  ACAGCCTGCCCCAGATCCCCATGCTAAACCTGGAGGCCCGGCGGTCCCACCTCTCAGAGAGCTGCAGCAT
  AGAGTCAGACATCTACGCAGAGATTCCCGACGAAACCCTGCGAAGGCCCGGAGGTCCACAGTATGGCATT
  GCCCGTGAAGATGTGGTCCTGAATCGTATTCTTGGGGAAGGCTTTTTTGGGGAGGTCTATGAAGGTGTCT
  ACACAAATCACAAAGGGGAGAAAATCAATGTAGCTGTCAAGACCTGCAAGAAAGACTGCACTCTGGACAA
  CAAGGAGAAGTTCATGAGCGAGGCAGTGATCATGAAGAACCTCGACCACCCGCACATCGTGAAGCTGATC
  GGCATCATTGAAGAGGAGCCCACCTGGATCATCATGGAATTGTATCCCTATGGGGAGCTGGGCCACTACC
  TGGAGCGGAACAAGAACTCCCTGAAGGTGCTCACCCTCGTGCTGTACTCACTGCAGATATGCAAAGCCAT
  GGCCTACCTGGAGAGCATCAACTGCGTGCACAGGGACATTGCTGTCCGGAACATCCTGGTGGCCTCCCCT
  GAGTGTGTGAAGCTGGGGGACTTTGGTCTTTCCCGGTACATTGAGGACGAGGACTATTACAAAGCCTCTG
  TGACTCGTCTCCCCATCAAATGGATGTCCCCAGAGTCCATTAACTTCCGACGCTTCACGACAGCCAGTGA
  CGTCTGGATGTTCGCCGTGTGCATGTGGGAGATCCTGAGCTTTGGGAAGCAGCCCTTCTTCTGGCTGGAG
  AACAAGGATGTCATCGGGGTGCTGGAGAAAGGAGACCGGCTGCCCAAGCCTGATCTCTGTCCACCGGTCC
  TTTATACCCTCATGACCCGCTGCTGGGACTACGACCCCAGTGACCGGCCCCGCTTCACCGAGCTGGTGTG
  CAGCCTCAGTGACGTTTATCAGATGGAGAAGGACATTGCCATGGAGCAAGAGAGGAATGCTCGCTACCGA
  ACCCCCAAAATCTTGGAGCCCACAGCCTTCCAGGAACCCCCACCCAAGCCCAGCCGACCTAAGTACAGAC
  CCCCTCCGCAAACCAACCTCCTGGCTCCAAAGCTGCAGTTCCAGGTTCCTGAGGGTCTGTGTGCCAGCTC
  TCCTACGCTCACCAGCCCTATGGAGTATCCATCTCCCGTTAACTCACTGCACACCCCACCTCTCCACCGG
  CACAATGTCTTCAAACGCCACAGCATGCGGGAGGAGGACTTCATCCAACCCAGCAGCCGAGAAGAGGCCC
  AGCAGCTGTGGGAGGCTGAAAAGGTCAAAATGCGGCAAATCCTGGACAAACAGCAGAAGCAGATGGTGGA
  GGACTACCAGTGGCTCAGGCAGGAGGAGAAGTCCCTGGACCCCATGGTTTATATGAATGATAAGTCCCCA
  TTGACGCCAGAGAAGGAGGTCGGCTACCTGGAGTTCACAGGGCCCCCACAGAAGCCCCCGAGGCTGGGCG
  CACAGTCCATCCAGCCCACAGCTAACCTGGACCGGACTGATGACCTGGTGTACCTCAATGTCATGGAGCT
  GGTGCGGGCCGTGCTGGAGCTCAAGAATGAGCTCTGTCAGCTGCCCCCCGAGGGCTACGTGGTGGTGGTG
  AAGAATGTGGGGCTGACCCTGCGGAAGCTCATCGGGAGCGTGGATGATCTCCTGCCTTCCTTGCCGTCAT
  CTTCACGGACAGAGATCGAGGGCACCCAGAAACTGCTCAACAAAGACCTGGCAGAGCTCATCAACAAGAT
  GCGGCTGGCACAGCAGAACGCCGTGACCTCCCTAAGTGAGGAGTGCAAGAGGCAGATGCTGACGGCTTCA
  CACACCCTGGCTGTGGACGCCAAGAACCTGCTCGACGCTGTGGACCAGGCCAAGGTTCTGGCCAATCTGG
  CCCACCCACCTGCAGAGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_173174 unedited
  CATTCCCCGCCCGCCGCCGCATTGGGCGGTAGGCGTGCACGGTGGGAGGTCTATATAAGC
  AGAGTTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCTGC
  GAATTCGGCACGAGGCTTTTACTCAGCCACAGCCTCCGGAGCCGTTGCACACCTACCTGC
  CCGGCCGACTTACCTGTACTTGCCGCCGTCCCGGCTCACCTGGCGGTGCCCGAGGAGTAG
  TCGCTGGAGTCCGCGCCTCCCTGGGACTGCAATGTGCCGGTCTTAGCTGCTGCCTGAGAG
  GATGTCTGGGGTGTCCGAGCCCCTGAGCCGAGTAAAGTTGGGCACATTACGCCGGCCTGA
  AGGCCCTGCAGAGCCCATGGTGGTGGTACCAGTAGATGTGGAAAAGGAGGACGTGCGTAT
  CCTCAAGGTCTGCTTCTATAGCAACAGCTTCAATCCTGGGAAGAACTTCAAACTGGTCAA
  ATGCACTGTCCAGACGGAGATCCGGGAGAGCATCACCTCCATCCTGCTGAGCGGGCGGAT
  CGGGCCCAACATCCGGATGGCTGAGTGCTATGGGCTGAGGCTGAAGCACATGAAGTCCGA
  TGAGATCCACTGGCTGCACCCACAGATGACGGTGGGTGAGGTGCAGGACAAGTATGAGTG
  TCTGCACGTGGAAGCCGAGTGGAGGTATGACCTTCAAATCCGCTACTTGCCAGCAGACTC
  CATGGAGAGCCTGCAAGAGGACAGGACCACGCTGCTCTATTTTTACCAACAGCTCCGAAC
  GACTACATGCAGCGCTACGCCAGCAGGTCACCGAGGGCATGGCCCTGCAGCTGGGCTGCC
  TGGAGCTCANGCGGTTCTCCAGGNATATGCCCCACATGCACTTGACAAGAAGTCCACTTC
  CAGCTCCTACAAAGGAAGGGGGCTGCCACTGTTTTTCCCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_173174 unedited
  TCCCCACCATNGACATGGGTGATGGCAATTCCAGGCCAGAATAGCACTGGGGAAGGGTAC
  ACAGGCATGCCACCCGGGTACTGTTCAGGAAAAGCTATGACCGCGGCCGCAATCTAGAGT
  CGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTNCAAANACAAGAAAAAAAAAGGTTTACGCAAACACAT
  GGAAAAAAAAAGGGGGAAACATAAAATGTGGGCGAGGGGAAAAAAAAAGGAGGACTTAAA
  AAAAAACTAAAGCAAGGGAAAGGAAAATAAGAAATATTGCATCTGCTTTGAGAAAAACAA
  GTTCAGCCCCATTTTTTATTTCTAAGAGCTGGAATTTGGACTTCCCGCCAAGTGCATTGT
  GGAGTACAACATATGAAGAACCGCCTGAGCTCCAGAAAGCCCAGCTGCAGGGACATGCCC
  TCACTGACTCTTGATGGAGAACAGATGCATGTAATTCCAACCGAAACTGTAGAAAAAAAA
  AAAGCAACGTGCGTCGTGTCCATATTCAGGCTCTACACGAAGACTTATGGCAAGGAGCGG
  ATTTTAAAAGAAAACCTCCACTCGGCTTCCACGTGCAGACAGTAATACTTGTCCTGGATC
  TCACAAACCGAAATCAGTGGGTGCAGCCAGAGGATCTCATCGGAATTCAAGTGCTTCAGC
  CGCAGCCCATAGCAATAAGCCAACCGCATGTTGGACCCGATCCGCCAGCTCAGCAGGAAG
  GAAGGGAAGATCCCCCGGGACTCCGGTCGGACAGTGCATTTGAACAGTTTGAAGTCCCTC
  CAAGAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_173174
• Insert Size: 4700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173174.1, NP_775266.1
• RefSeq Size: 4715 bp
• RefSeq ORF: 3030 bp
• Locus ID: 2185
• UniProt ID: Q14289
• Cytogenetics: 8p21.2
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Calcium signaling pathway, Chemokine signaling pathway, GnRH signaling pathway, Leukocyte transendothelial migration, Natural killer cell mediated cytotoxicity
• Gene Summary: This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1, 2, and 3 all encode isoform a.