IFITM2 (NM_006435) Human Untagged Clone

SKU SC124192

IFITM2 (untagged)-Human interferon induced transmembrane protein 2 (1-8D) (IFITM2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: IFITM2
• Synonyms: 1-8D; DSPA2c
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124192 sequence for NM_006435 edited (data generated by NextGen Sequencing); ATGAACCACATTGTGCAAACCTTCTCTCCTGTCAACAGCGGCCAGCCTCCCAACTACGAG; ATGCTCAAGGAGGAGCAGGAAGTGGCTATGCTGGGGGCGCCCCACAACCCTGCTCCCCCG; ATGTCCACCGTGATCCACATCCGCAGCGAGACCTCCGTGCCTGACCATGTCGTCTGGTCC; CTGTTCAACACCCTCTTCATGAACACCTGCTGCCTGGGCTTCATAGCATTCGCCTACTCC; GTGAAGTCTAGGGACAGGAAGATGGTTGGCGACGTGACCGGGGCCCAGGCCTATGCCTCC; ACCGCCAAGTGCCTGAACATCTGGGCCCTGATTTTGGGCATCTTCATGACCATTCTGCTC; ATCATCATCCCAGTGTTGGTCGTCCAGGCCCAGCGATAG; ; Clone variation with respect to NM_006435.2; 98 t=>c;171 g=>c;234 g=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006435 unedited; TTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCCGATCACCGCTGG; TCACCATGAACCACATTGTGCAAACCTTCTCTCCTGTCAACAGCGGCCAGCCTCCCAACT; ACGAGATGCTCAAGGAGGAGCAGGAAGTGGCTATGCTGGGGGCGCCCCACAACCCTGCTC; CCCCGATGTCCACCGTGATCCACATCCGCAGCGAGACCTCCGTGCCTGACCATGTCGTCT; GGTCCCTGTTCAACACCCTCTTCATGAACACCTGCTGCCTGGGCTTCATAGCATTCGCCT; ACTCCGTGAAGTCTAGGGACAGGAAGATGGTTGGCGACGTGACCGGGGCCCAGGCCTATG; CCTCCACCGCCAAGTGCCTGAACATCTGGGCCCTGATTTTGGGCATCTTCATGACCATTC; TGCTCATCATCATCCCAGTGTTGGTCGTCCAGGCCCAGCGATAGATCAGGAGGCATCATT; GAGGCCAGGAGCTCTGCCCGTGACCTGTATCCCACGTACTCTATCTTCCATTCCTCGCCC; TGCCCCCAGAGGCCAGGAGCTCTGCCCTTGACCTGTATTCCACTTACTCCACCTTCCATT; CCTCGCCCTGTCCCCACAGCCGAGTCCTGCATCAGCCCTTTATCCTCACACGCTTTTCTA; CAATGGCATTCAATAAAGTGTATANTGTTTCTGGTAAAAAAAAAAAAAAAAACTCGACTC; TAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCNTGTGAC; CCCTCCCCGTGCCTCTCCTGGCCCCTGAAGTTGCCACTNCAGTGCCCACCAGCCTTTGTC; TAATAAAATAAAGTGCATCATTTTGTCTGACTANGGTGTCTCATAATATGTGGGGGGGGG; GGGGGGGNGTGGNNNNNNNNNNNGGGAGGGGGNNNTNGGAGAGAAAACT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006435 unedited; NNTTATCTCTGNNACCGCGGCCCGCATTCTNANNATCGAGTTTTTTTTTTTTTTTTTTAC; CAAAAACATATACACTTTATTGAATGCCATTGTAGAAAAGCGTGTGAGGATAAAGGGCTG; ATGCAGGACTCGGCTGTGGGGACAGGGCGAGGAATGGAAGGTGGAGTAAGTGGAATACAG; GTCAAGGGCAGAGCTCCTGGCCTCTGGGGGCAGGGCGAGGAATGGAAGATAGAGTACGTG; GGATACAGGTCACGGGCAGAGCTCCTGGCCTCAATGATGCCTCCTGATCTATCGCTGGGC; CTGGACGACCAACACTGGGATGATGATGAGCAGAATGGTCATGAAGATGCCCAAAATCAG; GGCCCAGATGTTCAGGCACTTGGCGGTGGAGGCATAGGCCTGGGCCCCGGTCACGTCGCC; AACCATCTTCCTGTCCCTAGACTTCACGGAGTAGGCGAATGCTATGAAGCCCAGGCAGCA; GGTGTTCATGAAGAGGGTGTTGAACAGGGACCAGACGACATGGTCAGGCACGGAGGTCTC; GCTGCGGATGTGGATCACGGTGGACATCGGGGGAGCAGGGTTGTGGGGCGCCCCCAGCAT; AGCCACTTCCTGCTCCTCCTTGAGCATCTCGTANTTGGGAGGCTGGCCGCTGTTGACAGG; AGAGAAAGGTTGCACAATGTGGTTCATGGTGACCCAGCGTGATCGGGCCTCGTGCCGAAT; TCGCGGCCGCCCTATAGTGAGTCGTATTACAAATTCTGACGGTTCACTAAACGAGCTCTG; CTTTATAGACCTNCCACCGTACACGCCTACCGNCCATTTGCGTCACGGGGGCGGGGNTAT; TACCACATTTTGAAAGGCCCNGTGATTTTGGTGCCAAACAAACTCCNATTGACGTCAATG; GGGTGGAAACTTGGAAAATCCCGTGAGTCAAACCGTTATCACGCCCATG;
• Restriction Sites: NotI-NotI
• ACCN: NM_006435
• Insert Size: 670 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006435.1, NP_006426.1
• RefSeq Size: 905 bp
• RefSeq ORF: 399 bp
• Locus ID: 10581
• UniProt ID: Q01629
• Cytogenetics: 11p15.5
• Domains: CD225
• Protein Families: Transmembrane
• Summary: IFN-induced antiviral protein which inhibits the entry of viruses to the host cell cytoplasm, permitting endocytosis, but preventing subsequent viral fusion and release of viral contents into the cytosol. Active against multiple viruses, including influenza A virus, SARS coronavirus (SARS-CoV), Marburg virus (MARV), Ebola virus (EBOV), Dengue virus (DNV), West Nile virus (WNV), human immunodeficiency virus type 1 (HIV-1) and vesicular stomatitis virus (VSV). Can inhibit: influenza virus hemagglutinin protein-mediated viral entry, MARV and EBOV GP1,2-mediated viral entry, SARS-CoV S protein-mediated viral entry and VSV G protein-mediated viral entry. Induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner.[UniProtKB/Swiss-Prot Function]