Cathepsin L (CTSL) (NM_001912) Human Untagged Clone

CAT#: SC124186

CTSL1 (untagged)-Human cathepsin L1 (CTSL1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Cathepsin L
• Synonyms: CATL; CTSL1; MEP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124186 sequence for NM_001912 edited (data generated by NextGen Sequencing)
  ATGAATCCTACACTCATCCTTGCTGCCTTTTGCCTGGGAATTGCCTCAGCTACTCTAACA
  TTTGATCACAGTTTAGAGGCACAGTGGACCAAGTGGAAGGCGATGCACAACAGATTATAC
  GGCATGAATGAAGAAGGATGGAGGAGAGCAGTGTGGGAGAAGAACATGAAGATGATTGAA
  CTGCACAATCAGGAATACAGGGAAGGGAAACACAGCTTCACAATGGCCATGAACGCCTTT
  GGAGACATGACCAGTGAAGAATTCAGGCAGGTGATGAATGGCTTTCAAAACCGTAAGCCC
  AGGAAGGGGAAAGTGTTCCAGGAACCTCTGTTTTATGAGGCCCCCAGATCTGTGGATTGG
  AGAGAGAAAGGCTACGTGACTCCTGTGAAGAATCAGGGTCAGTGTGGTTCTTGTTGGGCT
  TTTAGTGCTACTGGTGCTCTTGAAGGACAGATGTTCCGGAAAACTGGGAGGCTTATCTCA
  CTGAGTGAGCAGAATCTGGTAGACTGCTCTGGGCCTCAAGGCAATGAAGGCTGCAATGGT
  GGCCTAATGGATTATGCTTTCCAGTATGTTCAGGATAATGGAGGCCTGGACTCTGAGGAA
  TCCTATCCATATGAGGCAACAGAAGAATCCTGTAAGTACAATCCCAAGTATTCTGTTGCT
  AATGACACCGGCTTTGTGGACATCCCTAAGCAGGAGAAGGCCCTGATGAAGGCAGTTGCA
  ACTGTGGGGCCCATTTCTGTTGCTATTGATGCAGGTCATGAGTCCTTCCTGTTCTATAAA
  GAAGGCATTTATTTTGAGCCAGACTGTAGCAGTGAAGACATGGATCATGGTGTGCTGGTG
  GTTGGCTACGGATTTGAAAGCACAGAATCAGATAACAATAAATATTGGCTGGTGAAGAAC
  AGCTGGGGTGAAGAATGGGGCATGGGTGGCTACGTAAAGATGGCCAAAGACCGGAGAAAC
  CATTGTGGAATTGCCTCAGCAGCCAGCTACCCCACTGTGTGA
  
  Clone variation with respect to NM_001912.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001912 unedited
  CCCCGTTCAGAATTGTATACGACTCATATAGGCGGCCTGCGAATCGGCACCAGGCAACCT
  TGAGCGGCATCCGTGGAGTGCGCCTGCGCAGCTACGACCGCAGCTAGGAAAGCGCCGCCG
  GCCAGGCCCAGCTGTGGCCGGACAGGGACTGGAAGAGAGGACGCGGTCGAGTAGGTGTGC
  ACCAGCCCTGGCAACGAGAGCGTCTACCCCGAACTCTGCTGGCCTTGAGGTGGGGAAGCC
  GGGGAGGGCAGTTGAGGACCCCGCGGAGGCGCGTGACTGGTTGAGCGGGCAGGCCAGCCT
  CCGAGCCGGGTGGACACAGGTTTTAAAACATGAATCCTACACTCATCCTTGCTGCCTTTT
  GCCTGGGAATTGCCTCAGCTACTCTAACATTTGATCACAGTTTAGAGGCACAGTGGACCA
  AGTGGAAGGCGATGCACAACAGATTATACGGCATGAATGAAGAAGGATGGAGGAGAGCAG
  TGTGGGAGAAGAACATGAAGATGATTGAACTGCACAATCAGGAATACAGGGAAGGGAAAC
  ACAGCTTCACAATGGCCATGAACGCCTTTGGAGACATGACCAGTGAAGAATTCAGGCAGG
  TGATGAATGGCTTTCAAAACCGTAAGCCCAGGAAGGGGTAAAGTGTTCCAGGAACCTCTG
  TTTTATGAGGCCCCCAGATCTGTGGATTGGAGAGAGAAAGGCTACGTGACTCCTGTGAAG
  AATAGGGTCAGTGTGGTTCTTGTTGNGCTATTAGTGCTACTGGTGCTCTTGAGGNACAGA
  TGTTCCGGACACTGNGAGGCTTATCTCACTGAGTGAGCAGCATCTGGTAGAGTGCTCTGG
  GCCTCACGGCATGACGGCTGCATGGTGGCTACTGGATATGCTTNCCAGTATGTCAGATTA
  ATGGAGCCTGGACTCTGAGAAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001912 unedited
  TCCAGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGG
  AAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTACATTT
  GAAATTAAATTTTATTTAAACAGGTAAAAATTTATACATCCTTTTAAAAACGAAAATGCA
  AAGTCAGTAAGTGAATCAATAATATAAACCTATTTATAGCAGTAATTAAAATAGAGGTAA
  CATTTACCAGTGTGAAAATATCACAGAATTCAAATCACACTCGGATCTTCAATGATTCGA
  GTGTGTATCCGACACAGCGGGGGCTGGTAGACTGAAGATGAATTCCTCCCATGCATGCGC
  CATCCCCAGTCAAGTCCTTCCTCATCACCGTCCACCAGCTCACACAGTGGGGTAGCTGGC
  TGCTGAGGCAATTCCACAATGGTTTCTCCGGTCTTTGGCCATCTTTACGTAGCCACCCAT
  GCCCCATTCTTCACCCCAGCTGTTCTTCACCAGCCAATATTTATTGTTATCTGATTCTGT
  GCTTTCAAATCCGTAGCCAACCACCAGCACACCATGATCCATGTCTTCACTGCTACAGTC
  TGGCTCAAAATAAATGCCTTCTTTATAGAACAGGAAGGACTCATGACCTGCATCAATAGC
  AACAGAAATGGGCCCCACAGTTGCAACTGCCTTCATCAGGGCCTTCTCCTGCTTAGGGAT
  GTCCACAAAGCCCGTGTCATTAGCAACAGAATN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001912
• Insert Size: 1600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001912.2, NP_001903.1
• RefSeq Size: 1632 bp
• RefSeq ORF: 1002 bp
• Locus ID: 1514
• UniProt ID: P07711
• Cytogenetics: 9q21.33
• Domains: Pept_C1
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Antigen processing and presentation, Lysosome
• Gene Summary: The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Additionally, this protein cleaves the S1 subunit of the SARS-CoV-2 spike protein, which is necessary for entry of the virus into the cell. [provided by RefSeq, Aug 2020]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1-4 encode the same isoform (1).