CHRNA6 (NM_004198) Human Untagged Clone

SKU SC124153

CHRNA6 (untagged)-Human cholinergic receptor, nicotinic, alpha 6 (CHRNA6), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: CHRNA6
• Synonyms: CHNRA6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124153 sequence for NM_004198 edited (data generated by NextGen Sequencing); ATGCTGACCAGCAAGGGGCAGGGATTCCTTCATGGGGGCTTGTGTCTCTGGCTGTGTGTG; TTCACACCTTTCTTTAAAGGCTGTGTGGGCTGTGCAACTGAGGAGAGGCTCTTCCACAAA; CTGTTTTCTCATTACAACCAGTTCATCAGGCCTGTGGAAAACGTTTCCGACCCTGTCACG; GTACACTTTGAAGTGGCCATCACCCAGCTGGCCAACGTGGATGAAGTAAACCAGATCATG; GAAACCAATTTGTGGCTGCGTCACATCTGGAATGATTATAAATTGCGCTGGGATCCAATG; GAATATGATGGCATTGAGACTCTTCGCGTTCCTGCAGATAAGATTTGGAAGCCCGACATT; GTTCTCTATAACAATGCTGTTGGTGACTTCCAAGTAGAAGGCAAAACAAAAGCTCTTCTT; AAATACAATGGCATGATAACCTGGACTCCACCAGCTATTTTTAAGAGTTCCTGCCCTATG; GATATCACCTTTTTCCCTTTTGATCATCAAAACTGTTCCCTAAAATTTGGTTCCTGGACG; TATGACAAAGCTGAAATTGATCTTCTAATCATTGGATCAAAAGTGGATATGAATGATTTT; TGGGAAAACAGTGAATGGGAAATCATTGATGCCTCTGGCTACAAACATGACATCAAATAC; AACTGTTGTGAAGAGATATACACAGATATAACCTATTCTTTCTACATTAGAAGATTGCCG; ATGTTTTACACGATTAATCTGATCATCCCTTGTCTCTTTATTTCATTTCTAACCGTGTTG; GTCTTTTACCTTCCTTCGGACTGTGGTGAAAAAGTGACGCTTTGTATTTCAGTCCTGCTT; TCTCTGACTGTGTTTTTGCTGGTCATCACAGAAACCATCCCATCCACATCTCTGGTGGTC; CCACTGGTGGGTGAGTACCTGCTGTTCACCATGATCTTTGTCACACTGTCCATCGTGGTG; ACTGTGTTTGTGTTGAACATACACTACCGCACCCCAACCACGCACACAATGCCCAGGTGG; GTGAAGACAGTTTTCCTGAAGCTGCTGCCCCAGGTCCTGCTGATGAGGTGGCCTCTGGAC; AAGACAAGGGGCACAGGCTCTGATGCAGTGCCCAGAGGCCTTGCCAGGAGGCCTGCCAAA; GGCAAGCTTGCAAGCCATGGGGAACCCAGACATCTTAAAGAATGCTTCCATTGTCACAAA; TCAAATGAGCTTGCCACAAGCAAGAGAAGATTAAGTCATCAGCCATTACAGTGGGTGGTG; GAAAATTCGGAGCACTCGCCTGAAGTTGAAGATGTGATTAACAGTGTTCAGTTCATAGCA; GAAAACATGAAGAGCCACAATGAAACCAAGGAGGTAGAAGATGACTGGAAATACGTGGCC; ATGGTGGTGGACAGAGTATTTCTTTGGGTATTTATAATTGTCTGTGTATTTGGAACTGCA; GGGCTATTTCTACAGCCACTACTTGGGAACACAGGAAAATCTTAA; ; Clone variation with respect to NM_004198.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004198 unedited; AAAGTTCGAATTTTGTAATACGACTCACTATAGNNGGCGGCCGCGNAATTNNCGGCACGA; GGCCCACAATCCTNNTTGCCTAGGAAAAAGGAATCCAAGTGTGTTTTAACCATGCTGACC; AGCAAGGGGCAGGGATTCCTTCATGGGGGCTTGTGTCTCTGGCTGTGTGTGTTCACACCT; TTCTTTAAAGGCTGTGTGGGCTGTGCAACTGAGGAGAGGCTCTTCCACAAACTGTTTTCT; CATTACAACCAGTTCATCAGGCCTGTGGAAAACGTTTCCGACCCTGTCACGGTACACTTT; GAAGTGGCCATCACCCAGCTGGCCAACGTGGATGAAGTAAACCAGATCATGGAAACCAAT; TTGTGGCTGCGTCACATCTGGAATGATTATAAATTGCGCTGGGATCCAATGGAATATGAT; GGCATTGAGACTCTTCGCGTTCCTGCAGATAAGATTTGGAAGCCCGACATTGTTCTCTAT; AACAATGCTGTTGGTGACTTCCAAGTAGAAAGCAAAACAAAAGCTCTTCTTAAATACAAT; GGCATGATAACCTGGACTCCACCAGCTATTTTTAAGAGTTCCTGCCCTATGGATATCACC; TTTTTCCCTTTTGATCATCAAAACTGGTCCCTAAAATTTGGTTCCTGGACGTATGACAAA; GCTGAAATTGAATCTCTAATCATTGGATCAAAAGTGGATATGAATGATTTTTGGGAAAAC; AGTGAATGGGGAAATCATTGATGCCTCTGGCTACAAAATGACATCAAATACAACTGGTGT; GAAGAGATATTCCCAGATTTACCCTATTCTTTCTACATATAAAGATTGGCAATGTTTTAC; ACGATTAAACTGAATCAACCCTTGGCCCTTTATTTTATTTTTCAACCGGGTGGNGCTTTT; ACCCTCCCCTCCGACCGGTGGTGAAAAAG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004198 unedited; AGGGGNNGTTGGTTNNNTTTGCATGCAGTGCACTTCCAGGCCAGGAGAGGCACTGGGGAG; GGGTCACAGGGATGCCACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATC; TAGAGTCGAGTTTTTTTTTTTTTTTTTTTAAAACTAACAATCAGTGTATCAAATAATGCT; GAGGAGGGTGAGTTACATTACAGAGACCTGCCCAAGAAATCAAGGATGCCATAGTTCACA; AAGTGTTGCAAACAGTTCACAAATGTTGCATGGTCCAATTCCCAGAGTTCACCTGTCACA; TGTCATAATACTGAGAAACAGCATCCACTGGAGGTACAGCAGAACTTTTTAGTGTGGCTT; GATGTTAATTACATAATTAGGCTGTATTGTAAGTCATTCTTGTCTTTAGAAGCCAAACAC; ACAGACACGAGAAACCTGACCTGATACTGTAGCCGTGGGTGCTGCCCAATCAGGGCACTA; ATGTCATAACACTTCTCACTTCTCCCCCTGTGCTACACTGGAGGATATTCTGCTTCCTAA; TGTGCAAGAAAGTCCTCTTTTTCCATGTAGCCATCAGTTTTAACAAATGGGGGACTTGGG; TGGGAAGCCTTTGCTTTCCTTTCCTTGGGGCCAGGAATGCAAAACAAATATGGGGGCTGG; AAATTTCTGAACATNAAAGAAAATACATTTTAAAAATTTTCTGTGTTCCCAATTATGGGC; TGTGAATAGCCCTGCATTTCCAAATCCCGACCATTATAATTCCCCAAGAAATACTCTGTC; ACCCCC;
• Restriction Sites: Please inquire
• ACCN: NM_004198
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004198.2, NP_004189.1
• RefSeq Size: 2090 bp
• RefSeq ORF: 1485 bp
• Locus ID: 8973
• UniProt ID: Q15825
• Cytogenetics: 8p11.21
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Summary: This gene encodes an alpha subunit of neuronal nicotinic acetylcholine receptors. These receptors consist of five subunits and function as ion channels involved in neurotransmission. The encoded protein is a subunit of neuronal nicotinic acetylcholine receptors that mediate dopaminergic neurotransmission and are activated by acetylcholine and exogenous nicotine. Alternatively spliced transcript variants have been observed for this gene. Single nucleotide polymorphisms in this gene have been associated with both nicotine and alcohol dependence. [provided by RefSeq, Dec 2010]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).