IL12B (NM_002187) Human Untagged Clone

CAT#: SC124116

IL12B (untagged)-Human interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) (IL12B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IL12B
• Synonyms: CLMF; CLMF2; IL-12B; IMD28; IMD29; NKSF; NKSF2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124116 sequence for NM_002187 edited (data generated by NextGen Sequencing)
  ATGTGTCACCAGCAGTTGGTCATCTCTTGGTTTTCCCTGGTTTTTCTGGCATCTCCCCTC
  GTGGCCATATGGGAACTGAAGAAAGATGTTTATGTCGTAGAATTGGATTGGTATCCGGAT
  GCCCCTGGAGAAATGGTGGTCCTCACCTGTGACACCCCTGAAGAAGATGGTATCACCTGG
  ACCTTGGACCAGAGCAGTGAGGTCTTAGGCTCTGGCAAAACCCTGACCATCCAAGTCAAA
  GAGTTTGGAGATGCTGGCCAGTACACCTGTCACAAAGGAGGCGAGGTTCTAAGCCATTCG
  CTCCTGCTGCTTCACAAAAAGGAAGATGGAATTTGGTCCACTGATATTTTAAAGGACCAG
  AAAGAACCCAAAAATAAGACCTTTCTAAGATGCGAGGCCAAGAATTATTCTGGACGTTTC
  ACCTGCTGGTGGCTGACGACAATCAGTACTGATTTGACATTCAGTGTCAAAAGCAGCAGA
  GGCTCTTCTGACCCCCAAGGGGTGACGTGCGGAGCTGCTACACTCTCTGCAGAGAGAGTC
  AGAGGGGACAACAAGGAGTATGAGTACTCAGTGGAGTGCCAGGAGGACAGTGCCTGCCCA
  GCTGCTGAGGAGAGTCTGCCCATTGAGGTCATGGTGGATGCCGTTCACAAGCTCAAGTAT
  GAAAACTACACCAGCAGCTTCTTCATCAGGGACATCATCAAACCTGACCCACCCAAGAAC
  TTGCAGCTGAAGCCATTAAAGAATTCTCGGCAGGTGGAGGTCAGCTGGGAGTACCCTGAC
  ACCTGGAGTACTCCACATTCCTACTTCTCCCTGACATTCTGCGTTCAGGTCCAGGGCAAG
  AGCAAGAGAGAAAAGAAAGATAGAGTCTTCACGGACAAGACCTCAGCCACGGTCATCTGC
  CGCAAAAATGCCAGCATTAGCGTGCGGGCCCAGGACCGCTACTATAGCTCATCTTGGAGC
  GAATGGGCATCTGTGCCCTGCAGTTAG
  
  Clone variation with respect to NM_002187.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002187 unedited
  CGCGGAAAGTCAGGATTTGTATCACGATCTCATATAGGCGGCCGCCAGTGTGATGGATAT
  CTGCAGAATTCGGCTTCAGAGCAAGATGTGTCACCAGCAGTTGGTCATCTCTTGGTTTTC
  CCTGGTTTTTCTGGCATCTCCCCTCGTGGCCATATGGGAACTGAAGAAAGATGTTTATGT
  CGTAGAATTGGATTGGTATCCGGATGCCCCTGGAGAAATGGTGGTCCTCACCTGTGACAC
  CCCTGAAGAAGATGGTATCACCTGGACCTTGGACCAGAGCAGTGAGGTCTTAGGCTCTGG
  CAAAACCCTGACCATCCAAGTCAAAGAGTTTGGAGATGCTGGCCAGTACACCTGTCACAA
  AGGAGGCGAGGTTCTAAGCCATTCGCTCCTGCTGCTTCACAAAAAGGAAGATGGAATTTG
  GTCCACTGATATTTTAAAGGACCAGAAAGAACCCAAAAATAAGACCTTTCTAAGATGCGA
  GGCCAAGAATTATTCTGGACGTTTCACCTGCTGGTGGCTGACGACAATCAGTACTGATTT
  GACATTCAGTGTCAAAAGCAGCAGAGGCTCTTCTGACCCCCAAGGGGTGACGTGCGGAGC
  TGCTACACTCTCTGCAGAGAGAGTCAGAGGGGACAACAAGGAGTATGAGTACTCAGTGGA
  GTGCCAGGAGGACAGTGCCTGCCCAGCTGCTGAGGAGAGTCTGCCCATTGAGGTCATGGT
  TGGATGCCGTTCACAAGCTCAAGTATGAAAACTACACCCGCAGCTTCTTCATCAGGGACA
  TCATCAAACCTGACCCACCCAAGAACTTGCAGCTGGAAGCCATTAAGGATTTCTCGCCGG
  TGGGAGGTCACCTTGGAAGTACCCTGACACCTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002187 unedited
  TGGGGNAGGGNNNNATGCACTGCATTCAACTTCCAGGCCGNANAACACTGGGGAGGGGTC
  ACAGGGATGCCACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAG
  TCGACAAGCTTGATATCGGTACCGAGCTCGGATCCACTAGTAACGGCCGCCAGTGTGCTG
  GAATTCGGCTTTTTCATCCTGGATCAGAACCTAACTGCAGGGCACAGATGCCCATTCGCT
  CCAAGATGAGCTATAGTAGCGGTCCTGGGCCCGCACGCTAATGCTGGCATTTTTGCGGCA
  GATGACCGTGGCTGAGGTCTTGTCCGTGAAGACTCTATCTTTCTTTTCTCTCTTGCTCTT
  GCCCTGGACCTGAACGCAGAATGTCAGGGAGAAGTAGGAATGTGGAGTACTCCAGGTGTC
  AGGGTACTCCCAGCTGACCTCCACCTGCCGAGAATTCTTTAATGGCTTCAGCTGCAAGTT
  CTTGGGTGGGTCAGGTTTGATGATGTCCCTGATGAAGAAGCTGCTGGTGTAGTTTTCATA
  CTTGAGCTTGTGAACGGCATCCACCATGACCTCAATGGGCAGACTCTCCTCAGCAGCTGG
  GCAGGCACTGTCCTCCTGGCACTCCACTGAGTACTCATACTCCTTGTTGTCCCCTCTGAC
  TCTCTCTGCAGAGAGTGTAGCAGCTCCGCACGTCACCCCTTTGGGGTCAGAAGAGCCTCT
  GCTGCTTTGACACTGAATGTCAAATCAGTACTGATTTGCGTCAGCCACCAGCAGGTGAAA
  CGTCCAGAATAATTCTTGGCTN
  
• Restriction Sites: Please inquire
• ACCN: NM_002187
• Insert Size: 1100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002187.2, NP_002178.2
• RefSeq Size: 2347 bp
• RefSeq ORF: 987 bp
• Locus ID: 3593
• UniProt ID: P29460
• Cytogenetics: 5q33.3
• Domains: FN3
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Allograft rejection, Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus
• Gene Summary: This gene encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children. [provided by RefSeq, Jul 2008]