CD16 (FCGR3A) (NM_000569) Human Untagged Clone

CAT#: SC124061

FCGR3A (untagged)-Human Fc fragment of IgG, low affinity IIIa, receptor (CD16a) (FCGR3A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD16
• Synonyms: CD16; CD16A; FCG3; FCGR3; FCGRIII; FCR-10; FCRIII; FCRIIIA; IGFR3; IMD20
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124061 sequence for NM_000569 edited (data generated by NextGen Sequencing)
  ATGGGTGGAGGGGCTGGGGAAAGGCTGTTTACTTCCTCCTGTCTAGTCGGTTTGGTCCCT
  TTAGGGCTCCGGATATCTTTGGTGACTTGTCCTCTCCAGTGTGGCATCATGTGGCAGCTG
  CTCCTCCCAACTGCTCTGCTACTTCTAGTTTCAGCTGGCATGCGGACTGAAGATCTCCCA
  AAGGCTGTGGTGTTCCTGGAGCCTCAATGGTACAGGGTGCTCGAGAAGGACAGTGTGACT
  CTGAAGTGCCAGGGAGCCTACTCCCCTGAGGACAATTCCACACAGTGGTTTCACAATGAG
  AGCCTCATCTCAAGCCAGGCCTCGAGCTACTTCATTGACGCTGCCACAGTCGACGACAGT
  GGAGAGTACAGGTGCCAGACAAACCTCTCCACCCTCAGTGACCCGGTGCAGCTAGAAGTC
  CATATCGGCTGGCTGTTGCTCCAGGCCCCTCGGTGGGTGTTCAAGGAGGAAGACCCTATT
  CACCTGAGGTGTCACAGCTGGAAGAACACTGCTCTGCATAAGGTCACATATTTACAGAAT
  GGCAAAGGCAGGAAGTATTTTCATCATAATTCTGACTTCTACATTCCAAAAGCCACACTC
  AAAGACAGCGGCTCCTACTTCTGCAGGGGGCTTGTTGGGAGTAAAAATGTGTCTTCAGAG
  ACTGTGAACATCACCATCACTCAAGGTTTGGCAGTGTCAACCATCTCATCATTCTTTCCA
  CCTGGGTACCAAGTCTCTTTCTGCTTGGTGATGGTACTCCTTTTTGCAGTGGACACAGGA
  CTATATTTCTCTGTGAAGACAAACATTCGAAGCTCAACAAGAGACTGGAAGGACCATAAA
  TTTAAATGGAGAAAGGACCCTCAAGACAAATGA
  
  Clone variation with respect to NM_000569.6
  93 a=>t;634 t=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000569 unedited
  NNCCCAACGCCTTCACGATTTGTGAAACGACTTCTATAGGCGGCACGCGATTCCCGGGCA
  GATTCTGTGTGTGTCCTCAGCAGCTCAGCCACAGACCTTTGAGGGAGTAAAGGGGGCAGA
  CCCACCCACCTTGCCTCCAGGCTCTTTCCTTCCTGGTCCTGTTCTATGGTGGGGCTCCCT
  TGCCAGACTTCAGACTGAGAAGTCAGATGAAGTTTCAAGAAAAGGAAATTGGTGGGTGAC
  AGAGATGGGTGGAGGGGCTGGGGAAAGGCTGTTTACTTCCTCCTGTCTAGTCAGTTTGGT
  CCCTTTAAGGCTCCGGATATCTTTGGTGACTTGTCCTCTCCAGTGTGGCATCATGTGGCA
  GCTGCTCCTCCCAACTGCTCTGCTACTTCTAGTTTCAGCTGGCATGCGGACTGAAGATCT
  CCCAAAGGCTGTGGTGTTCCTGGAGCCTCAATGGTACAGGGTGCTCGAGAAAGACAGTGT
  GACTCTGAAGTGCCAGGGAGCCTACTCCCCTGAGGACAATTCCACACAGTGGTTTCACAA
  TGAGAGCCTCATCTCAAGCCAGGCCTCGAGCTACTTCATTGACGCTGCCACAGTCGACGA
  CAGTGGAGAGTACAGGTGCCAGACAAACCTCTCCACCCTCAGTGACCCGGTGCAGCTAGA
  AGTCCATATCGGCTGGCTGTTGCTCCAGGCCCCTCGGTGGGTGTTCAAGGAGGAAGACCC
  TATTCACCTGAAGTGTCACAGCTGGAAGAACACTGCTCTGCATAAAGTCACATATTTACA
  AGATGGCAAAAGCAGGAAGTATTTTCATCATAATTCTGACTTCTACATTCCAAAAGCCAC
  ACTCAAAGACAGCGGCTCCTACTTCTGCAAGGGAGCTTGTTGGGAGTAAAAATGTGTCTT
  CAGAAACTGTGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000569 unedited
  NAGGGTCAAGGGCTGCCACCCGGGTATCTGTTCAAGAAAACAGCTATGACCGCGGCCGCA
  ATCTACAGCGGCCGCCCTCTTTTTTNTTTTTTATTTATTTTTTATTTTTTCTTTTTTTAT
  CATTCATATTTTATTACCATGGTTTTCCCATCTTCTATCTAAAAGTAACATGCAATTTTT
  TTTAAAATGCTTTATTGGAACCAAAAAATGTTGCGCTTAAAGCTTACAAAACAAAGACAG
  CTAAAGCTTTCTTTCATAAACAACAATTGTCTTCTCCATCCCCACCTTATTGGAACTGAC
  ATGATGAAAGATTTGAAAGTTTCATAACTTAACTCAGCGAAGCTCAGTAGTACATTTAGT
  ATTGGTTATACAACATTTGTTTAATAAATGCAATGAACAAAGCTACACAGGAATTATATA
  TTGAAGCAAAAAAAGTGGTTTTACAGTCCCTGCATTAACCTCTAATTCTTACTACCCTGG
  CCAAAAAAGCATTTTCACCTCCTGCGCTTTCCTTCCTGTGTGCTTGAGGTTGGTTCTTTC
  TTCTCAAGCTTTCTCATTCTGATGCTGAGATAGTTCTGTTCACTTAGCAACTTGGGACAG
  AGACACAAGGTTTGTTCTGTACTTTCTTTTCCACCCCACCCCCCACCGAAATCCTCAATC
  CCCTTCCTAAGACCATTTTCTACTCCTAACATTAAAAAGACTCATCGTTATATACTTGAG
  AAATGGTCCAATTCTTATAAAATCCCCCTGGAAGACTCTATTTCTACCTCCCCCCCATGA
  ATTTCCTGGTAATTCCCCCTACGGTTATAGTTTTAAGAAAACACCCTTTTTTCTGGGGCT
  AAGATGGAAACCACCCCCCTTAAACTATCCTGGACT
  
• Restriction Sites: Please inquire
• ACCN: NM_000569
• Insert Size: 2100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000569.6, NP_000560.5
• RefSeq Size: 2406 bp
• RefSeq ORF: 873 bp
• Locus ID: 2214
• UniProt ID: P08637
• Cytogenetics: 1q23.3
• Domains: ig, IG
• Protein Families: ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
• Protein Pathways: Fc gamma R-mediated phagocytosis, Natural killer cell mediated cytotoxicity, Systemic lupus erythematosus
• Gene Summary: This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other responses, including antibody dependent cellular mediated cytotoxicity and antibody dependent enhancement of virus infections. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene are associated with immunodeficiency 20, and have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2020]
  Transcript Variant: This variant (1) retains an intron in its 5' UTR and 5' coding region compared to variant 3. The encoded isoform (a) has a longer and distinct N-terminus compared to isoform c. This isoform (a) lacks a predicted signal peptide compared to isoform c.