KCNA5 (NM_002234) Human Untagged Clone

CAT#: SC123964

KCNA5 (untagged)-Human potassium voltage-gated channel, shaker-related subfamily, member 5 (KCNA5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KCNA5
• Synonyms: ATFB7; HCK1; HK2; HPCN1; KV1.5; PCN1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002234, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGATCGCCCTGGTGCCCCTGGAGAACGGCGGTGCCATGACCGTCAGAGGAGGCGATGAGGCCCGGG
  CAGGCTGCGGCCAGGCCACAGGGGGAGAGCTCCAGTGTCCCCCGACGGCTGGGCTCAGCGATGGGCCCAA
  GGAGCCGGCGCCAAAGGGGCGCGGCGCGCAGAGAGACGCGGACTCGGGAGTGCGGCCCTTGCCTCCGCTG
  CCGGACCCGGGAGTGCGGCCCTTGCCTCCGCTGCCAGAGGAGCTGCCACGGCCTCGACGGCCGCCTCCCG
  AGGACGAGGAGGAAGAAGGCGATCCCGGCCTGGGCACGGTGGAGGACCAGGCTCTGGGCACGGCGTCCCT
  GCACCACCAGCGCGTCCACATCAACATCTCCGGGCTGCGCTTTGAGACGCAGCTGGGCACCCTGGCGCAG
  TTCCCCAACACACTCCTGGGGGACCCCGCCAAGCGCCTGCGCTACTTCGACCCCCTGAGGAACGAGTACT
  TCTTCGACCGCAACCGGCCCAGCTTCGACGGTATCCTCTACTACTACCAGTCCGGGGGCCGCCTGCGGAG
  GCCGGTCAACGTCTCCCTGGACGTGTTCGCGGACGAGATACGCTTCTACCAGCTGGGGGACGAGGCCATG
  GAGCGCTTCCGCGAGGATGAGGGCTTCATTAAAGAAGAGGAGAAGCCCCTGCCCCGCAACGAGTTCCAGC
  GCCAGGTGTGGCTTATCTTCGAGTATCCGGAGAGCTCTGGGTCCGCGCGGGCCATCGCCATCGTCTCGGT
  CTTGGTTATCCTCATCTCCATCATCACCTTCTGCTTGGAGACCCTGCCTGAGTTCAGGGATGAACGTGAG
  CTGCTCCGCCACCCTCCGGCGCCCCACCAGCCTCCCGCGCCCGCCCCTGGGGCCAACGGCAGCGGGGTCA
  TGGCCCCGCCCTCTGGCCCTACGGTGGCACCGCTCCTGCCCAGGACCCTGGCCGACCCCTTCTTCATCGT
  GGAGACCACGTGCGTCATCTGGTTCACCTTCGAGCTGCTCGTGCGCTTCTTCGCCTGCCCCAGCAAGGCA
  GGGTTCTCCCGGAACATCATGAACATCATCGATGTGGTGGCCATCTTCCCCTACTTCATCACCCTGGGCA
  CCGAACTGGCAGAGCAGCAGCCAGGGGGTGGAGGAGGCGGCCAGAATGGGCAGCAGGCCATGTCCCTGGC
  CATCCTCCGAGTCATCCGCCTGGTCCGGGTGTTCCGCATCTTCAAGCTCTCCCGCCACTCCAAGGGGCTG
  CAGATCCTGGGCAAGACCTTGCAGGCCTCCATGAGGGAGCTGGGGCTGCTCATCTTCTTCCTCTTCATCG
  GGGTCATCCTCTTCTCCAGTGCCGTCTACTTCGCAGAGGCTGACAACCAGGGAACCCATTTCTCTAGCAT
  CCCTGACGCCTTCTGGTGGGCAGTGGTCACCATGACCACTGTGGGCTACGGGGACATGAGGCCCATCACT
  GTTGGGGGCAAGATCGTGGGCTCGCTGTGTGCCATCGCCGGGGTCCTCACCATTGCCCTGCCTGTGCCCG
  TCATCGTCTCCAACTTCAACTACTTCTACCACCGGGAAACGGATCACGAGGAGCCGGCAGTCCTTAAGGA
  AGAGCAGGGCACTCAGAGCCAGGGGCCGGGGCTGGACAGAGGAGTCCAGCGGAAGGTCAGCGGGAGCAGG
  GGATCCTTCTGCAAGGCTGGGGGGACCCTGGAGAATGCAGACAGTGCCCGAAGGGGCAGCTGCCCCCTAG
  AGAAGTGTAACGTCAAGGCCAAGAGCAACGTGGACTTGCGGAGGTCCCTTTATGCCCTCTGCCTGGACAC
  CAGCCGGGAAACAGATTTGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002234 unedited
  NTTTCCCTGGAGAACGCGGTGCCATGACCGTCAGAGGAGGCGATGAGGCCCGGGCAGGCT
  GCGGCCAGGCCACAGGGGGAGAGCTCCAGTGTCCCCCGACGGCTGGGCTCAGCGATGGGC
  CCAAGGAGCCGGCGCCAAAGGGGCGCGGCGCGCAGAGAGACGCGGACTCGGGAGTGCGGC
  CCTTGCCTCCGCTGCCGGACCCGGGAGTGCGGCCCTTGCCTCCGCTGCCAGAGGAGCTGC
  CACGGCCTCGACGGCCGCCTCCCGAGGACGAGGAGGAAGAAGGCGATCCCGGCCTGGGCA
  CGGTGGAGGACCAGGCTCTGGGCACGGCGTCCCTGCACCACCAGCGCGTCCACATCAACA
  TCTCCGGGCTGCGCTTTGAGACGCAGCTGGGCACCCTGGCGCAGTTCCCCAACACACTCC
  TGGGGGACCCCGCCAAGCGCCTGCGCTACTTCGACCCCCTGAGGAACGAGTACTTCTTCG
  ACCGCAACCGGCCCAGCTTCGACGGTATCCTCTACTACTACCAGTCCGGGGGCCGCCTGC
  GGAGGCCGGTCAACGTCTCCCTGGACGTGTTCGCGGACGAGATACGCTTCTACCAGCTGG
  GGGACGAGGCCATGGAGCGCTTCCGCGAGGATGAGGGCTTCATTAAAGAAGAGGAGAAGC
  CCCTGCCCCGCAACGAGTTCCAGCGCCAGGTGTGGCTTATCNTCGAGTATCCGGAGAGCT
  CTGGGTCCGCGCGGGCCATCGCCATCGTCTCGGTCTTGGTTATCTCATCTCCTCATCACC
  TTCTGCTTGAGACCNTGCTGAGTTAGGGATGACGTGAGCTGCTCCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002234 unedited
  NTTGCCTGGATGGCACTTCCAGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCA
  CCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTCTT
  TCTTTGGGTTTTTTTGCAACAAGGTAACGGTTTATTTCCTGGGATAAAAATAAAGACAAT
  AAATTATCAGAAGTATTCCTCCAATATCATTAAAATGCTTTTTGAAGAAAGTTACAAGAA
  TTGTTTAAGGCGCACAGAAAAAGTCAGATTTGCCTTTCTTCAAGAACATACTCTGCCCTA
  GACACAAAGCACCAATACCACATCCCAGAAAAGACAGAGGCTTGGAGACACATGAAACAG
  ATCAGCCCATACCAAGATCTCTGACTCTAATATAAGTAACACAGAAAAACATCGGGACAA
  CACTTTCTTCCATAAATACAACATGTATCTTGAAAATATACCCAAAATATCTCTATCTGA
  ACTACAGTACAAAAAGCCACTTACCAGGCATAAAAATAATATTTTCCCCTATACACGAAT
  ACAATCATATGTCAAGCAAATTTGGAACACATTGATGGGAGAGTTACATAATCTCTTTGG
  ATCTGGGCTATTTAGAGTCATTTTTGTTTGGGGTATGCCTCTGCTTGGGTATACGAGACT
  GGCTACAAATACATNCATTCCTAAAGCGGTTAACAATTTCAACAACCTCTCGGTTATTGG
  AATGGGGTACTTTTTAAAAATAAGGCACACCTTGGTGTGTTGGAAAGGAATTATCAAATT
  GGGGGGGTCTCGGGGGGGGGGGAAAAAGGTTTAAAGGGAGGGGGTATCTGNG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002234
• Insert Size: 3000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002234.2, NP_002225.2
• RefSeq Size: 2865 bp
• RefSeq ORF: 1842 bp
• Locus ID: 3741
• UniProt ID: P22460
• Cytogenetics: 12p13.32
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: Potassium channels represent the most complex class of voltage-gated ino channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. It belongs to the delayed rectifier class, the function of which could restore the resting membrane potential of beta cells after depolarization and thereby contribute to the regulation of insulin secretion. This gene is intronless, and the gene is clustered with genes KCNA1 and KCNA6 on chromosome 12. Defects in this gene are a cause of familial atrial fibrillation type 7 (ATFB7). [provided by RefSeq, May 2012]