MMP13 (NM_002427) Human Untagged Clone

CAT#: SC123911

MMP13 (untagged)-Human matrix metallopeptidase 13 (collagenase 3) (MMP13)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MMP13
• Synonyms: CLG3; MANDP1; MDST; MMP-13
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC123911 sequence for NM_002427 edited (data generated by NextGen Sequencing)
  ATGCATCCAGGGGTCCTGGCTGCCTTCCTCTTCTTGAGCTGGACTCATTGTCGGGCCCTG
  CCCCTTCCCAGTGGTGGTGATGAAGATGATTTGTCTGAGGAAGACCTCCAGTTTGCAGAG
  CGCTACCTGAGATCATACTACCATCCTACAAATCTCGCGGGAATCCTGAAGGAGAATGCA
  GCAAGCTCCATGACTGAGAGGCTCCGAGAAATGCAGTCTTTCTTCGGCTTAGAGGTGACT
  GGCAAACTTGACGATAACACCTTAGATGTCATGAAAAAGCCAAGATGCGGGGTTCCTGAT
  GTGGGTGAATACAATGTTTTCCCTCGAACTCTTAAATGGTCCAAAATGAATTTAACCTAC
  AGAATTGTGAATTACACCCCTGATATGACTCATTCTGAAGTCGAAAAGGCATTCAAAAAA
  GCCTTCAAAGTTTGGTCCGATGTAACTCCTCTGAATTTTACCAGACTTCACGATGGCATT
  GCTGACATCATGATCTCTTTTGGAATTAAGGAGCATGGCGACTTCTACCCATTTGATGGG
  CCCTCTGGCCTGCTGGCTCATGCTTTTCCTCCTGGGCCAAATTATGGAGGAGATGCCCAT
  TTTGATGATGATGAAACCTGGACAAGTAGTTCCAAAGGCTACAACTTGTTTCTTGTTGCT
  GCGCATGAGTTCGGCCACTCCTTAGGTCTTGACCACTCCAAGGACCCTGGAGCACTCATG
  TTTCCTATCTACACCTACACCGGCAAAAGCCACTTTATGCTTCCTGATGACGATGTACAA
  GGGATCCAGTCTCTCTATGGTCCAGGAGATGAAGACCCCAACCCTAAACATCCAAAAACG
  CCAGACAAATGTGACCCTTCCTTATCCCTTGATGCCATTACCAGTCTCCGAGGAGAAACA
  ATGATCTTTAAAGACAGATTCTTCTGGCGCCTGCATCCTCAGCAGGTTGATGCGGAGCTG
  TTTTTAACGAAATCATTTTGGCCAGAACTTCCCAACCGTATTGATGCTGCATATGAGCAC
  CCTTCTCATGACCTCATCTTCATCTTCAGAGGTAGAAAATTTTGGGCTCTTAATGGTTAT
  GACATTCTGGAAGGTTATCCCAAAAAAATATCTGAACTGGGTCTTCCAAAAGAAGTTAAG
  AAGATAAGTGCAGCTGTTCACTTTGAGGATACAGGCAAGACTCTCCTGTTCTCAGGAAAC
  CAGGTCTGGAGATATGATGATACTAACCATATTATGGATAAAGACTATCCGAGACTAATA
  GAAGAAGACTTCCCAGGAATTGGTGATAAAGTAGATGCTGTCTATGAGAAAAATGGTTAT
  ATCTATTTTTTCAACGGACCCATACAGTTTGAATACAGCATCTGGAGTAACCGTATTGTT
  CGCGTCATGCCAGCAAATTCCATTTTGTGGTGTTAA
  
  Clone variation with respect to NM_002427.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002427 unedited
  NAACGTACAAATTTTGNTAATACGACTCACTATAGGGCGGCCGCGATTCGGCACGAGATT
  CAAGAGCATCCAGGGGTCCTGGCTGCCTTCCTCTTCTTGAGCTGGACTCATTGTCGGGCC
  CTGCCCCTTCCCAGTGGTGGTGATGAAGATGATTTGTCTGAGGAAGACCTCCAGTTTGCA
  GAGCGCTACCTGAGATCATACTACCATCCTACAAATCTCGCGGGAATCCTGAAGGAGAAT
  GCAGCAAGCTCCATGACTGAGAGGCTCCGAGAAATGCAGTCTTTCTTCGGCTTAGAGGTG
  ACTGGCAAACTTGACGATAACACCTTAGATGTCATGAAAAAGCCAAGATGCGGGGTTCCT
  GATGTGGGTGAATACAATGTTTTCCCTCGAACTCTTAAATGGTCCAAAATGAATTTAACC
  TACAGAATTGTGAATTACACCCCTGATATGACTCATTCTGAAGTCGAAAAGGCATTCAAA
  AAAGCCTTCAAAGTTTGGTCCGATGTAACTCCTCTGAATTTTACCAGACTTCACGATGGC
  ATTGCTGACATCATGATCTCTTTTGGAATTAAGGAGCATGGCGACTTCTACCCATTTGAT
  GGGCCCTCTGGCCTGCTGGCTCATGCTTTTCCTCCTGGGCCAAATTATGGAGGAGATGCC
  CATTTTGATGATGATGAAACCTGGACAAGTAGTTCCAAAGGCTACAACTTGTTTCTTGTT
  GCTGCGCATGAGTTCGGCCACTCCTTTAGTCTTGACCACTCCAAGGACCCTGGAGCACTC
  ATGTTTTCTATCTACACCTACACCGGGCAAAGCCACTTTTTGCTTCCTGATGACGATGTC
  CAAGGGAATCCGTCTCCCTAATGGTCCGGGAGATGAAGACCCCCACCCTAAACATC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002427 unedited
  TCCAGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGG
  AAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTGTTTTTTTTTTTTTT
  TTCTGTTGAAAATATATTTTTATTATAAACAACATAAACACTTCCTAATACACTTTGCAA
  ATATGCATTTCATTTTCTTTGCCTGAGTTTTTATCAACAGTGTCTCTGAGCACAATAGTT
  CTTCCCTTGATGGCCGATCATATATTCAATAAGTGCCAAGCACCCTCCCCAAGTATCAAT
  AGGCACTGTGGGAAGTGCTGGGGGATTTTTTTAAATGCAGAGAAAAAAAGGCATCCTTCC
  ATATCTCAAGGGATCACAATCTAGTAGACTGCTTTAAAAAAAAATTTGGATGTCTCTTTT
  TTAATATACATACTTCCACTTTATAAGATATATTTTATTTCATGCTATACAAGTCTTTAG
  AAAATAGCCTATATATCCAAGACAAACTGGACAATTAAATAATTAGCTAGGTAAAATAGG
  GTTAAAATGCAACTTTTAGTCTTAAATAATCAAATAATTTATGAAAAAGGGATGCCATGG
  CCTTAGACTATTTTGATAACAATTTTAACTTTGTAATGTGTGACTTCTGAAGAACCTGAA
  ATATTTTAATAAAAAGACATCATCCTTCTTTATCCTTTATAATATGCAAAGGAATATTTG
  TCTTATATGAGAGCAGACTTTGAGTCATTGCCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002427
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002427.2, NP_002418.1
• RefSeq Size: 2722 bp
• RefSeq ORF: 1416 bp
• Locus ID: 4322
• UniProt ID: P45452
• Cytogenetics: 11q22.2
• Protein Families: Druggable Genome, Protease
• Gene Summary: This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. Mutations in this gene are associated with metaphyseal anadysplasia. This gene is part of a cluster of MMP genes on chromosome 11. [provided by RefSeq, Jan 2016]