Mineralocorticoid Receptor (NR3C2) (NM_000901) Human Untagged Clone

CAT#: SC123888

NR3C2 (untagged)-Human nuclear receptor subfamily 3, group C, member 2 (NR3C2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Mineralocorticoid Receptor
• Synonyms: MCR; MLR; MR; NR3C2VIT
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  GAATTCGGCACCAGTTCCCCTTCCCCCGCCCCAGCCTCCCCACCATGTCCTAGAAAAGCGATGGAGACC
  AAAGGCTACCACAGTCTCCCTGAAGGTCTAGATATGGAAAGACGGTGGGGTCAAGTTTCTCAGGCTGTG
  GAGCGTTCTTCCCTGGGACCTACAGAGAGGACCGATGAGAATAACTACATGGAGATTGTCAACGTAAGC
  TGTGTTTCCGGTGCTATTCCAAACAACAGTACTCAAGGAAGCAGCAAAGAAAAACAAGAACTACTCCCT
  TGCCTTCAGCAAGACAATAATCGGCCTGGGATTTTAACATCTGATATTAAAACTGAGCTGGAATCTAAG
  GAACTTTCAGCAACTGTAGCTGAGTCCATGGGTTTATATATGGATTCTGTAAGAGATGCTGACTATTCC
  TATGAGCAGCAGAACCAACAAGGAAGCATGAGTCCAGCTAAGATTTATCAGAATGTTGAACAGCTGGTG
  AAATTTTACAAAGGAAATGGCCATCGTCCTTCCACTCTAAGTTGTGTGAACACGCCCTTGAGATCATTT
  ATGTCTGACTCTGGGAGCTCCGTGAATGGTGGCGTCATGCGCGCCGTTGTTAAAAGCCCTATCATGTGT
  CATGAGAAAAGCCCGTCTGTTTGCAGCCCTCTGAACATGACATCTTCGGTTTGCAGCCCTGCTGGAATC
  AACTCTGTGTCCTCCACCACAGCCAGCTTTGGCAGTTTTCCAGTGCACAGCCCAATCACCCAGGGAACT
  CCTCTGACATGCTCCCCTAATGTTGAAAATCGAGGCTCCAGGTCGCACAGCCCTGCACATGCTAGCAAT
  GTGGGCTCTCCTCTCTCAAGTCCGTTAAGTAGCATGAAATCCTCAATTTCCAGCCCTCCAAGTCACTGC
  AGTGTAAAATCTCCAGTCTCCAGTCCCAATAATGTCACTCTGAGATCCTCTGTGTCTAGCCCTGCAAAT
  ATTAACAACTCAAGGTGCTCTGTTTCCAGCCCTTCGAACACTAATAACAGATCCACGCTTTCCAGTCCG
  GCAGCCAGTACTGTGGGATCTATCTGTAGCCCTGTAAACAATGCCTTCAGCTACACTGCTTCTGGCACC
  TCTGCTGGATCCAGTACATTGCGGGATGTGGTTCCCAGTCCAGACACGCAGGAGAAAGGTGCTCAAGAG
  GTCCCTTTTCCTAAGACTGAGGAAGTAGAGAGTGCCATCTCAAATGGTGTGACTGGCCAGCTTAATATT
  GTCCAGTACATAAAACCAGAACCAGATGGAGCTTTTAGCAGCTCATGTCTAGGAGGAAATAGCAAAATA
  AATTCGGATTCTTCATTCTCAGTACCAATAAAGCAAGAATCAACCAAGCATTCATGTTCAGGCACCTCT
  TTTAAAGGGAATCCAACAGTAAACCCGTTTCCATTTATGGATGGCTCGTATTTTTCCTTTATGGATGAT
  AAAGACTATTATTCCCTATCAGGAATTTTAGGACCACCTGTGCCCGGCTTTGATGGTAACTGTGAAGGC
  AGCGGATTCCCAGTGGGTATTAAACAAGAACCAGATGATGGGAGCTATTACCCAGAGGCCAGCATCCCT
  TCCTCTGCTATTGTTGGGGTGAATTCAGGTGGACAGTCCTTCCACTACAGGATTGGTGCTCAAGGTACA
  ATATCTTTATCACGATCGGCTAGAGACCAATCTTTCCAACACCTGAGTTCCTTTCCTCCTGTCAATACT
  TTAGTGGAGTCATGGAAATCACACGGCGACCTGTCGTCTAGAAGAAGTGATGGGTATCCGGTCTTAGAA
  TACATTCCAGAAAATGTATCAAGCTCTACTTTACGAAGTGTTTCTACTGGATCTTCAAGACCTTCAAAA
  ATATGTTTGGTGTGTGGGGATGAGGCTTCAGGATGCCATTATGGGGTAGTCACCTGTGGCAGCTGCAAA
  GTTTTCTTCAAAAGAGCAGTGGAAGGGCAACACAACTATTTATGTGCTGGAAGAAATGATTGCATCATT
  GATAAGATTCGACGAAAGAATTGTCCTGCTTGCAGACTTCAGAAATGTCTTCAAGCTGGAATGAATTTA
  GGAGCACGAAAGTCAAAGAAGTTGGGAAAGTTAAAAGGGATTCACGAGGAGCAGCCACAGCAGCAGCAG
  CCCCCACCCCCACCCCCACCCCCGCAAAGCCCAGAGGAAGGGACAACGTACATCGCTCCTGCAAAAGAA
  CCCTCGGTCAACACAGCACTGGTTCCTCAGCTCTCCACAATCTCACGAGCGCTCACACCTTCCCCCGTT
  ATGGTCCTTGAAAACATTGAACCTGAAATTGTATATGCAGGCTATGACAGCTCAAAACCAGATACAGCC
  GAAAATCTGCTCTCCACGCTCAACCGCTTAGCAGGCAAACAGATGATCCAAGTCGTGAAGTGGGCAAAG
  GTACTTCCAGGATTTAAAAACTTGCCTCTTGAGGACCAAATTACCCTAATCCAGTATTCTTGGATGTGT
  CTATCATCATTTGCCTTGAGCTGGAGATCGTACAAACATACGAACAGCCAATTTCTCTATTTTGCACCA
  GACCTAGTCTTTAATGAAGAGAAGATGCATCAGTCTGCCATGTATGAACTATGCCAGGGGATGCACCAA
  ATCAGCCTTCAGTTCGTTCGACTGCAGCTCACCTTTGAAGAATACACCATCATGAAAGTTTTGCTGCTA
  CTAAGCACAATTCCAAAGGATGGCCTCAAAAGCCAGGCTGCATTTGAAGAAATGAGGACAAATTACATC
  AAAGAACTGAGGAAGATGGTAACTAAGTGTCCCAACAATTCTGGGCAGAGCTGGCAGAGGTTCTACCAA
  CTGACCAAGCTGCTGGACTCCATGCATGACCTGGTGAGCGACCTGCTGGAATTCTGCTTCTACACCTTC
  CGAGAGTCCCATGCGCTGAAGGTAGAGTTCCCCGCAATGCTGGTGGAGATCATCAGCGACCAGCTGCCC
  AAGGTGGAGTCGGGGAACGCCAAGCCGCTCTACTTCCACCGGAAGTGACTGCCCGCTGCCCAGAAGAAC
  TTTGCCTTAAGTTTCCCTGTGTTGTTCCACACCCAGAAGGACCCAAGAAAACCTGTTTTTAACATGTGA
  TGGTTGATTC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000901 unedited
  ATAGGGCGGCCGCGAATTCGGCACCAGTTCCCCTTCCCCCGCCCCAGCCTCCCCACCATG
  TCCTAGAAAAGCGATGGAGACCAAAGGCTACCACAGTCTCCCTGAAGGTCTAGATATGGA
  AAGACGGTGGGGTCAAGTTTCTCAGGCTGTGGAGCGTTCTTCCCTGGGACCTACAGAGAG
  GACCGATGAGAATAACTACATGGAGATTGTCAACGTAAGCTGTGTTTCCGGTGCTATTCC
  AAACAACAGTACTCAAGGAAGCAGCAAAGAAAAACAAGAACTACTCCCTTGCCTTCAGCA
  AGACAATAATCGGCCTGGGATTTTAACATCTGATATTAAAACTGAGCTGGAATCTAAGGA
  ACTTTCAGCAACTGTAGCTGAGTCCATGGGTTTATATATGGATTCTGTAAGAGATGCTGA
  CTATTCCTATGAGCAGCAGAACCAACAAGGAAGCATGAGTCCAGCTAAGATTTATCAGAA
  TGTTGAACAGCTGGTGAAATTTTACAAAGGAAATGGCCATCGTCCTTCCACTCTAAGTTG
  TGTGAACACGCCCTTGAGATCATTTATGTCTGACTCTGGGAGCTCCGTGAATGGTGGCGT
  CATGCGCGCCGTTGTTAAAAGCCCTATCATGTGTCATGAGAAAAGCCCGTCTGTTTGCAG
  CCCTCTGAACATGACATCTTCGGTTTGCAGCCCTGCTGGAATCAACTCTGTGTCCTCCAC
  CACAGCCAGCTTTGGCAGTTCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000901 unedited
  GGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGGAAACAGCTA
  TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGATTAAAGTAAAGGA
  TTTATTGTAATCTGCTTACAGTCCTTTGCAAAGACAGACATATGTTTTTGCATAAAGATA
  TAAATTGCTTCATTTTAAACTAATTTAGTGTTTTTTTAAATTATATGAACTTTTGGTGAA
  TTATGAACTGTACCAAACCAAGATTTTTAAGAGCAATATGGTACAAAAATAAGAGCAGAC
  AGACAATTTGGACTGGGACAGGCATTCAACAGTGAATTTAGAATATGGCTTTGCTGTTTA
  ATCAAGCAAAGTTACCTGGAGACACACAAAGAGCCAGTAACTTTGTTAATACAAAAAGCT
  GATGCAGACCATCTCCATCCCAAACCTGGTTAAGTAAGTCAGAATTCCCATTTTATAAAA
  ACTAAGTCTGAACTAAAAGCTGCTTTTGTAAGTAAGCCATTTGTTTGCTAGCAACTCCCA
  GTCTGCATGCTGTGTATTTATTACCTTCAAAGTGTGGCTTTCATATTACTGTGCACCGTA
  AGTTGACTGTATTTAAAGATCTGCCTATAAATTAAATTCCTGAAATTGCAAATTATGTAA
  GAAAAACTCCTCCCTCTGAGTGCAGCTTTCACAGGTTTCCCCTTGCATAAGGCAGGTGTC
  TTTTGAAAGACAAGGTAATG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000901
• Insert Size: 3155 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000901.1
• RefSeq Size: 5749 bp
• RefSeq ORF: 3155 bp
• Locus ID: 4306
• UniProt ID: P08235
• Cytogenetics: 4q31.23
• Domains: HOLI, zf-C4
• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• MW: 114.9 kDa
• Gene Summary: This gene encodes the mineralocorticoid receptor, which mediates aldosterone actions on salt and water balance within restricted target cells. The protein functions as a ligand-dependent transcription factor that binds to mineralocorticoid response elements in order to transactivate target genes. Mutations in this gene cause autosomal dominant pseudohypoaldosteronism type I, a disorder characterized by urinary salt wasting. Defects in this gene are also associated with early onset hypertension with severe exacerbation in pregnancy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.