CYP7A1 (NM_000780) Human Untagged Clone

CAT#: SC123882

CYP7A1 (untagged)-Human cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CYP7A1
• Synonyms: CP7A; CYP7; CYPVII
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000780 edited
  CGGCCGCGAATTCGGCACCAGGCTTTGTCAACCAAGCTCAAGTTAATGGATCTGGATACT
  ATGTATATAAAAAGCCTAGCTTGAGTCTCTTTTCAGTGGCATCCTTCCCTTTCTAATCAG
  AGATTTTCTTCCTCAGAGATTTTGGCCTAGATTTGCAAAATGATGACCACATCTTTGATT
  TGGGGGATTGCTATAGCAGCATGCTGTTGTCTATGGCTTATTCTTGGAATTAGGAGAAGG
  CAAACGGGTGAACCACCTCTAGAGAATGGATTAATTCCATACCTGGGCTGTGCTCTGCAA
  TTTGGTGCCAATCCTCTTGAGTTCCTCAGAGCAAATCAAAGGAAACATGGTCATGTTTTT
  ACCTGCAAACTAATGGGAAAATATGTCCATTTCATCACAAATCCCTTGTCATACCATAAG
  GTGTTGTGCCACGGAAAATATTTTGATTGGAAAAAATTTCACTTTGCTACTTCTGCGAAG
  GCATTTGGGCACAGAAGCATTGACCCGATGGATGGAAATACCACTGAAAACATAAACGAC
  ACTTTCATCAAAACCCTGCAGGGCCATGCCTTGAATTCCCTCACGGAAAGCATGATGGAA
  AACCTCCAACGTATCATGAGACCTCCAGTCTCCTCTAACTCAAAGACCGCTGCCTGGGTG
  ACAGAAGGGATGTATTCTTTCTGCTACCGAGTGATGTTTGAAGCTGGGTATTTAACTATC
  TTTGGCAGAGATCTTACAAGGCGGGACACACAGAAAGCACATATTCTAAACAATCTTGAC
  AACTTCAAGCAATTCGACAAAGTCTTTCCAGCCCTGGTAGCAGGCCTCCCCATTCACATG
  TTCAGGACTGCGCACAATGCCCGGGAGAAACTGGCAGAGAGCTTGAGGCACGAGAACCTC
  CAAAAGAGGGAAAGCATCTCAGAACTGATCAGCCTGCGCATGTTTCTCAATGACACTTTG
  TCCACCTTTGATGATCTGGAGAAGGCCAAGACACACCTCGTGGTCCTCTGGGCATCGCAA
  GCAAACACCATTCCAGCGACTTTCTGGAGTTTATTTCAAATGATTAGGAACCCAGAAGCA
  ATGAAAGCAGCTACTGAAGAAGTGAAAAGAACATTAGAGAATGCTGGTCAAAAAGTCAGC
  TTGGAAGGCAATCCTATTTGTTTGAGTCAAGCAGAACTGAATGACCTGCCAGTATTAGAT
  AGTATAATCAAGGAATCGCTGAGGCTTTCCAGTGCCTCCCTCAACATCCGGACAGCTAAG
  GAGGATTTCACTTTGCACCTTGAGGACGGTTCCTACAACATCCGAAAAGATGACATCATA
  GCTCTTTACCCACAGTTAATGCACTTAGATCCAGAAATCTACCCAGACCCTTTGACTTTT
  AAATATGATAGGTATCTTGATGAAAACGGGAAGACAAAGACTACCTTCTATTGTAATGGA
  CTCAAGTTAAAGTATTACTACATGCCCTTTGGATCGGGAGCTACAATATGTCCTGGAAGA
  TTGTTCGCTATCCACGAAATCAAGCAATTTTTGATTCTGATGCTTTCTTATTTTGAATTG
  GAGCTTATAGAGGGCCAAGCTAAATGTCCACCTTTGGACCAGTCCCGGGCAGGCTTGGGC
  ATTTTGCCGCCATTGAATGATATTGAATTTAAATATAAATTCAAGCATTTGTGAATACAT
  GGCTGGAATAAGAGGACACTAGATGATATTACAGGACTGCAGAACACCCTCACCACACAG
  TCCCTTTGGACAAATGCATTTAGTGGTGGTAGAAATGATTCACCAGGTCCAATGTTGTTC
  ACCAGTGCTTGCTTGTGAATCTTAACATTTTGGTGACAGTTTCCAGATGCTATCACAGAC
  TCTGCTAGTGAAAAGAACTAGTTTCTAGGAGCACAATAATTTGTTTTCATTTGTATAAGT
  CCATGAATGTTCATATAGCCAGGGATTGAAGTTTATTATTTTCAAAGGAAAACACCTTTA
  TTTTATTTTTTTTCAAAATGAAGATACACATTACAGCCAGGTGTGGTAGCAGGCACCTGT
  AGTCTTAGCTACTCGAGAGGCCA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000780 unedited
  CCGTCAGATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACCAGGCTTTGTCA
  ACCAAGCTCAAGTTAATGGATCTGGATACTATGTATATAAAAAGCCTAGCTTGAGTCTCT
  TTTCAGTGGCATCCTTCCCTTTCTAATCAGAGATTTTCTTCCTCAGAGATTTTGGCCTAG
  ATTTGCAAAATGATGACCACATCTTTGATTTGGGGGATTGCTATAGCAGCATGCTGTTGT
  CTATGGCTTATTCTTGGAATTAGGAGAAGGCAAACGGGTGAACCACCTCTAGAGAATGGA
  TTAATTCCATACCTGGGCTGTGCTCTGCAATTTGGTGCCAATCCTCTTGAGTTCCTCAGA
  GCAAATCAAAGGAAACATGGTCATGTTTTTACCTGCAAACTAATGGGAAAATATGTCCAT
  TTCATCACAAATCCCTTGTCATACCATAAGGTGTTGTGCCACGGAAAATATTTTGATTGG
  AAAAAATTTCACTTTGCTACTTCTGCGAAGGCATTTGGGCACAGAAGCATTGACCCGATG
  GATGGAAATACCACTGAAAACATAAACGACACTTTCATCAAAACCCTGCAGGGCCATGCC
  TTGAATTCCCTCACGGAAAGCATGATGGANAACCTCCAACGTATCATGAGACCTCCAGTC
  TCCTCTAACTCAAAGACCGCTGCCTGGGTGACAGAAGGGATGTATTCTTTCTGCTACCGA
  GTGATGTTTGAAGCTGGGTATTTAACTATCTTTGGGCAGAGATCTTACAAGGCGGGACAC
  ACAAGAAGCACATATTCTAAACAATCTTGACAACTTTCAGCAATTCGACAAAGTCTTTCA
  GCCCTGGTAGCACGCCTCCCCATTTCCCTGTTCAGGACTGCGCACAATGCCCGGGAAGAA
  CTTGCAGAGAGCTTGGAGGCCCAAGACCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000780 unedited
  GGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGGAAACAGCTA
  TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTAGATTCATACTTTTATTTCTGAAAGATGAATCATTTCAAAATTATCAAATGTT
  AGAATATTTTCTTAATATTTGTAAAATAAGCAAAAAGGTAAATATAATTTAAATATAAAT
  CCTACCCAGAAATATCAATTGACATAGAGCTGATAACTCATACTTAAAATATTTTATCAA
  AGCATTGTTGTACACAGCATTTAAACACTCGAGTCACCTTAGTTTTTTTCATCTGCAAGT
  CATTTAGCGATAAAAAGCATTTAAATAAAACATAATGTCAAGCTGCACTTAATACACTCC
  ACATGTTTCATAACCAAAGCTTCCAAATGGCTATTTCCATCCAGTTTTAACTTTAAAACA
  TATCACATAAAAGCTACATTTTTTCAGTGATAATGTGTATTTAACAATGACAGAAAAACT
  ACCTTTTTTAATCTCTGAGAAAACTCTTCTCATTAATGTGAATGAAATAAAAATATAGAG
  AAGATAGTTAAAGCCGGACATAGAGTTTTCAGCACAATAAGTTAAAACATGAAGGGAAAA
  TAAAACCACCTTAGTGTTTTTGGAAATCAAGATATCATGTTTAAGCCCTGATTTTTGATA
  TAGAGAAGTGAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_000780
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000780.2, NP_000771.1
• RefSeq Size: 2877 bp
• RefSeq ORF: 1515 bp
• Locus ID: 1581
• UniProt ID: P22680
• Cytogenetics: 8q12.1
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, P450, Transmembrane
• Protein Pathways: Metabolic pathways, PPAR signaling pathway, Primary bile acid biosynthesis
• Gene Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway in the liver, which converts cholesterol to bile acids. This reaction is the rate limiting step and the major site of regulation of bile acid synthesis, which is the primary mechanism for the removal of cholesterol from the body. Polymorphisms in the promoter of this gene are associated with defects in bile acid synthesis. [provided by RefSeq, Feb 2010]