CYP11B1 (NM_000497) Human Untagged Clone

CAT#: SC123879

CYP11B1 (untagged)-Human cytochrome P450, family 11, subfamily B, polypeptide 1 (CYP11B1), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CYP11B1
• Synonyms: CPN1; CYP11B; FHI; P450C11
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000497 edited
  GGGCGGCCGCGAATTCGGCACCAGATGGCACTCAGGGCAAAGGCAGAGGTGTGCATGGCA
  GTGCCCTGGCTGTCCCTGCAAAGGGCACAGGCACTGGGCACGAGAGCCGCCCGGGTCCCC
  AGGACAGTGCTGCCCTTTGAAGCCATGCCCCGGCGTCCAGGCAACAGGTGGCTGAGGCTG
  CTGCAGATCTGGAGGGAGCAGGGTTATGAGGACCTGCACCTGGAAGTACACCAGACCTTC
  CAGGAACTAGGGCCCATTTTCAGGTACGACTTGGGAGGAGCAGGCATGGTGTGTGTGATG
  CTGCCGGAGGACGTGGAGAAGCTGCAACAGGTGGACAGCCTGCATCCCCACAGGATGAGC
  CTGGAGCCCTGGGTGGCCTACAGACAACATCGTGGGCACAAATGTGGCGTGTTCTTGCTG
  AATGGGCCTGAATGGCGCTTCAACCGATTGCGGCTGAATCCAGAAGTGCTGTCGCCCAAC
  GCTGTGCAGAGGTTCCTCCCGATGGTGGATGCAGTGGCCAGGGACTTCTCCCAGGCCCTG
  AAGAAGAAGGTGCTGCAGAACGCCCGGGGGAGCCTGACCCTGGACGTCCAGCCCAGCATC
  TTCCACTACACCATAGAAGCCAGCAACTTGGCTCTTTTTGGAGAGCGGCTGGGCCTGGTT
  GGCCACAGCCCCAGTTCTGCCAGCCTGAACTTCCTCCATGCCCTGGAGGTCATGTTCAAA
  TCCACCGTCCAGCTCATGTTCATGCCCAGGAGCCTGTCTCGCTGGACCAGCCCCAAGGTG
  TGGAAGGAGCACTTTGAGGCCTGGGACTGCATCTTCCAGTACGGCGACAACTGTATCCAG
  AAAATCTATCAGGAACTGGCCTTCAGCCGCCCTCAACAGTACACCAGCATCGTGGCAGAG
  CTCCTGTTGAATGCGGAACTGTCGCCAGATGCCATCAAGGCCAACTCTATGGAACTCACT
  GCAGGGAGCGTGGACACGACGGTGTTTCCCTTGCTGATGACGCTCTTTGAGCTGGCTCGG
  AACCCCAACGTGCAGCAGGCCCTGCGCCAGGAGAGCCTGGCCGCCGCAGCCAGCATCAGT
  GAACATCCCCAGAAGGCAACCACCGAGCTGCCCTTGCTGCGTGCGGCCCTCAAGGAGACC
  TTGCGGCTCTACCCTGTGGGTCTGTTTCTGGAGCGAGTGGCGAGCTCAGACTTGGTGCTT
  CAGAACTACCACATCCCAGCTGGGACATTGGTGCGCGTGTTCCTCTACTCTCTGGGTCGC
  AACCCCGCCTTGTTCCCGAGGCCTGAGCGCTATAACCCCCAGCGCTGGCTAGACATCAGG
  GGCTCCGGCAGGAACTTCTACCACGTGCCCTTTGGCTTTGGCATGCGCCAGTGCCTTGGG
  CGGCGCCTGGCAGAGGCAGAGATGCTGCTGCTGCTGCACCATGTGCTGAAACACCTCCAG
  GTGGAGACACTAACCCAAGAGGACATAAAGATGGTCTACAGCTTCATATTGAGGCCCAGC
  ATGTTCCCCCTCCTCACCTTCAGAGCCATCAACTAATCACGTCTCTGCACCCAGGGTCCC
  AGCCTGGCCACCAGCCTCCCTTTCTGCCTGACCCCAGGCCACCCCTCTTCTCTCCCACAT
  GCACAGCTTCCTGAGTCACCCCTCTGTCTAACCAGCCCCAGCACAAATGGAACTCCCGAG
  GGCCTCTAGGACCAGGGTTTGCCAGGCTAAGCAGCAATGCCAGGGCACAGCTGGGGAAGA
  TCTTGCTGACCTTGTCCCCAGCCCCACCTGGCCCTTTCTCCAGCAAGCACTGTCCTCTGG
  GCAGTTTGCCCCCATCCCTCCCAGTGCTGGCTCCAGGCTCCTCGTGTGGCCATGCAAGGG
  TGCTGTGGTTTTGTCCCTTGCCTTCCTGCCTAGTCTCACATGTCC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000497 unedited
  ACGTCAAAATTTGTAAACGACTCATATAGGGCGGCCGCGAATTCGCACCAGNAGGCACTC
  AGGGCAAAGGCAGAGGTGTGCATGGCAGTGCCCTGGCTGTCCCTGCAAAGGGCACAGGCA
  CTGGGCACGAGAGCCGCCCGGGTCCCCAGGACAGTGCTGCCCTTTGAAGCCATGCCCCGG
  CGTCCAGGCAACAGGTGGCTGAGGCTGCTGCAGATCTGGAGGGAGCAGGGTTATGAGGAC
  CTGCACCTGGAAGTACACCAGACCTTCCAGGAACTAGGGCCCATTTTCAGGTACGACTTG
  GGAGGAGCAGGCATGGTGTGTGTGATGCTGCCGGAGGACGTGGAGAAGCTGCAACAGGTG
  GACAGCCTGCATCCCCACAGGATGAGCCTGGAGCCCTGGGTGGCCTACAGACAACATCGT
  GGGCACAAATGTGGCGTGTTCTTGCTGAATGGGCCTGAATGGCGCTTCAACCGATTGCGG
  CTGAATCCAGAAGTGCTGTCGCCCAACGCTGTGCAGAGGTTCCTCCCGATGGTGGATGCA
  GTGGCCAGGGACTTCTCCCAGGCCCTGAAGAAGAAGGTGCTGCAGAACGCCCGGNGGAGC
  CTGACCCTGGACGTCCAGCCCAGCATCTTCCACTACACCATAGAAGCCAGCAACTTGGCT
  CTTTTTGGAGAGCGGCTGGGCCTGGTTGGCCACAGCCNCAGTTCTGCCAGCCTGAACTTC
  CTCCATGCCCTGGAGGTCATGTTCAAATCCACCGTCCAGCTCATGTTCATGCCCAGGAGC
  CTGTCTCGCTNGACCAGCCCCAAGGTGTGGNAAGGAGCACTTTGAGGCCTGGGACTGCAT
  CTTCCAGTACGGGCGACACTGTATCCAGAAAATCTATCAGGAACTGGCCTTCAGCCGCCC
  TCACAGTACACCAGCATCGTGGCAGAGCTCCTGTTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000497 unedited
  GGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGGAAACAGCTA
  TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTAGAGAAACAGAAGCCGAGTAGTTTAATCTGAATTTTTGGGTCCG
  CCTCCAGAATTCATAGGCTGAAATCTAGTCACCAAGGGGACGATAATAGCAGGGCAGGGC
  CTTTGAGGGGGGAAGGGAATGCAGGCCGTGCCCTCTTGAATGGAACTGGCGTCCTTATAA
  AAAAGGTCTCTGTGAGCTGTCTTGCCCTTTTCCACCATGTGAGGACACAGCAAGAAGGTT
  TTATCTATGAAACAAGAGGTGGGTCCTCACCAGACACAGAACCTGGTGACACCTTGATCT
  TAGCCTTCTAAGCCTTCAGAACCATGAGAAATAGAATTCTGATGTTTGTCAGCAACCCAG
  TCTATGGCGTTTTCTTAGAGCATCCTGAATAGATTAACACAAATAACTCTAGCTAGGTGT
  GCTCCCAACCTGGGGCACTTCATCTCCCTGTACAGGCTGAGTCCTGCAGGGCGTCCTGGA
  TCTACTGACCACCACTGGAATGATGCCATAGGGATTTCCCCTTTCATGGGGGAACAAATG
  CTCTGATCCTCATCAGCCACCAGGAGCTGGCTGCTGTGATCTTTGCCCTGATGAAAATCC
  ATGGAGATAAAGACAGTCT
  
• Restriction Sites: Please inquire
• ACCN: NM_000497
• Insert Size: 3600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000497.3, NP_000488.3
• RefSeq Size: 3551 bp
• RefSeq ORF: 1512 bp
• Locus ID: 1584
• UniProt ID: P15538
• Cytogenetics: 8q24.3
• Protein Families: Druggable Genome, P450
• Protein Pathways: Androgen and estrogen metabolism, C21-Steroid hormone metabolism, Metabolic pathways
• Gene Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and is involved in the conversion of progesterone to cortisol in the adrenal cortex. Mutations in this gene cause congenital adrenal hyperplasia due to 11-beta-hydroxylase deficiency. Transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longer isoform (1).