CYP27B1 (NM_000785) Human Untagged Clone

SKU SC123873

CYP27B1 (untagged)-Human cytochrome P450, family 27, subfamily B, polypeptide 1 (CYP27B1), nuclear gene encoding mitochondrial protein

Product Data

• Type: Human Untagged Clone
• Target Symbol: CYP27B1
• Synonyms: CP2B; CYP1; CYP1alpha; CYP27B; P450c1; PDDR; VDD1; VDDR; VDDRI; VDR
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000785 edited; GGCGGCCGCGAATTCGGCACGAGGCTGAACCAGACCATGACCCAGACCCTCAAGTACGCC; TCCAGAGTGTTCCATCGCGTCCGCTGGGCGCCCGAGTTGGGCGCCTCCCTAGGCTACCGA; GAGTACCACTCAGCACGCCGGAGCTTGGCAGACATCCCAGGCCCCTCTACGCCCAGCTTT; CTGGCCGAACTTTTCTGCAAGGGGGGGCTGTCGAGGCTACACGAGCTGCAGGTGCAGGGC; GCCGCGCACTTCGGGCCGGTGTGGCTAGCCAGCTTTGGGACAGTGCGCACCGTGTACGTG; GCTGCCCCTGCACTCGTCGAGGAGCTGCTGCGACAGGAGGGACCCCGGCCCGAGCGCTGC; AGCTTCTCGCCCTGGACGGAGCACCGCCGCTGCCGCCAGCGGGCTTGCGGACTGCTCACT; GCGGAAGGCGAAGAATGGCAAAGGCTCCGCAGTCTCCTGGCCCCGCTCCTCCTCCGGCCT; CAAGCGGCCGCCCGCTACGCCGGAACCCTGAACAACGTAGTCTGCGACCTTGTGCGGCGT; CTGAGGCGCCAGCGGGGACGTGGCACGGGGCCGCCCGCCCTGGTTCGGGACGTGGCGGGG; GAATTTTACAAGTTCGGACTGGAAGGCATCGCCGCGGTTCTGCTCGGCTCGCGCTTGGGC; TGCCTGGAGGCTCAAGTGCCACCCGACACGGAGACCTTCATCCGCGCTGTGGGCTCGGTG; TTTGTGTCCACGCTGTTGACCATGGCGATGCCCCACTGGCTGCGCCACCTTGTGCCTGGG; CCCTGGGGCCGCCTCTGCCGAGACTGGGACCAGATGTTTGCATTTGCTCAGAGGCACGTG; GAGCGGCGAGAGGCAGAGGCAGCCATGAGGAACGGAGGACAGCCCGAGAAGGACCTGGAG; TCTGGGGCGCACCTGACCCACTTCCTGTTCCGGGAAGAGTTGCCTGCCCAGTCCATCCTG; GGAAATGTGACAGAGTTGCTATTGGCGGGAGTGGACACGGTGTCCAACACGCTCTCTTGG; GCTCTGTATGAGCTCTCCCGGCACCCCGAAGTCCAGACAGCACTCCACTCAGAGATCACA; GCTGCCCTGAGCCCTGGCTCCAGTGCCTACCCCTCAGCCACTGTTCTGTCCCAGCTGCCC; CTGCTGAAGGCGGTGGTCAAGGAAGTGCTAAGACTGTACCCTGTGGTACCTGGAAATTCT; CGTGTCCCAGACAAAGACATTCATGTGGGTGACTATATTATCCCCAAAAATACGCTGGTC; ACTCTGTGTCACTATGCCACTTCAAGGGACCCTGCCCAGTTCCCAGAGCCAAATTCTTTT; CGTCCAGCTCGCTGGCTGGGGGAGGGTCCCACCCCCCACCCATTTGCATCTCTTCCCTTT; GGCTTTGGCAAGCGCAGCTGTATGGGGAGACGCCTGGCAGAGCTTGAATTGCAAATGGCT; TTGGCCCAGATCCTAACACATTTTGAGGTGCAGCCTGAGCCAGGTGCGGCCCCAGTTAGA; CCCAAGACCCGGACTGTCCTGGTACCTGAAAGGAGCATCAACCTACAGTTTTTGGACAGA; TAGTCCCATGGAAAGAGACTGTCATCATCACCCTTTCATTCATCATAGGGATAAGATTTT; TTGTAGGCACAAGACCAAGGTATACATCTTCCCCTAATGCCTATCTGACCAAACTGGATA; GAACCACCATAGTGAAGTGTGAGGCGGCCCTGACCAATGTGTGAAGTATGCACTTGGCCT; GACTCAGGAAGCCAGGTGAGAAAACCATGGTCTCTCTGCTTGCTTGGCCCTTCTGATCAT; GTATGCATCCCCCAAGGATGAAATCAGATTTTAACTAATAATGCTGGATGGCCTGAGGAA; AGATTCAACTGCCTCTCTTTTTGGGCTTTCATAGTGTTCATTGATGCTGCTGGCTAAGCA; TTTATCAAAGCATAAGCTCAGTAACTGTGCATCTGGTCTGTACCTGGTTGGTCCTTCGTC; TTTGCATGTAAGCTCTTTGAGAGGAAGGGTGAAGCCTTATTTGTTTTTTATGTCCCCTGC; CAGGGCCTGTCTCTGACTAGGTGTCACCATACACATTCTTAGATTGAATCTGAACCATGT; GGCAGAAGGGATAAGCAGCTTACTTAGTAGGCTCTGTCTACCCCCTTCCTTCTTTGTCTT; GCCCCTAGGAAGGTGAATCTGCCCTAGCCTGGTTTACGGTTTCTTATAACTCTCCTTTGC; TCTCTGGCCACTATTAAGTGGGTTTGCCCCATCACTTAGTTCTCAGGCAGAGACATCTTT; GGGCCTGTCCCTGCCCAGGCCTCTGGCTTTTTATATTGAAAATTTTTAAATATTCACAAA; TTTTAGAATAAATCAAATATTCCATTAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGC; GGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCC; AGTGCCTC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000785 unedited; NGGGTTCCCAATTTGTATACGACNACTATAGGCGGCCGCGNAATTCGCACGAGGCTGAAC; CAGACCTGACCCAGACCCTCAGTACGCCTCCAGAGTGTTCCATCGCGTCCGCTGGGCGCC; CGAGTTGGGCGCCTCCCTAGGCTACCGAGAGTACCACTCAGCACGCCGGAGCTTGGCAGA; CATCCCAGGCCCCTCTACGCCCAGCTTTCTGGCCGAACTTTTCTGCAAGGGGGGGCTGTC; GAGGCTACACGAGCTGCAGGTGCAGGGCGCCGCGCACTTCGGGCCGGTGTGGCTAGCCAG; CTTTGGGACAGTGCGCACCGTGTACGTGGCTGCCCCTGCACTCGTCGAGGAGCTGCTGCG; ACAGGAGGGACCCCGGCCCGAGCGCTGCAGCTTCTCGCCCTGGACGGAGCACCGCCGCTG; CCGCCAGCGGGCTTGCGGACTGCTCACTGCGGAAGGCGAAGAATGGCAAAGGCTCCGCAG; TCTCCTGGCCCCGCTCCTCCTCCGGCCTCAAGCGGCCGCCCGCTACGCCGGAACCCTGAA; CAACGTAGTCTGCGACCTTGTGCGGCGTCTGAGGCGCCAGCGGGGACGTGGCACGGGGCC; GCCCGCCCTGGTTCGGGACGTGGCGGGGGAATTTTACAAGTTCGGACTGGAAGGCATCGC; CGCGGTTCTGCTCGGCTCGCGCTTGGGCTGCCTGGAGGCTCAAGTGCCACCCGACACGGA; GACCTTCATCCGCGCTGTGGGCTCGGTGTTTGTGTCCACGCTGTTGACCATGGCGATGCC; CCACTGGCTGCGCCACCTTGTGCCTGGGCCCTGGGGCCGCCTCTGCCGAGACTGGGACCA; GATGTTTGCATTTGCTCAGAGCACNNTGGAGCGCNAGAGGCANAAGCAGCCATNGAGAAC; GGNAGACAGCCCGAGAAG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000785 unedited; GGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGGAAACAGCTA; TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAATGGAATATTTGAT; TTATTCTAAAATTTGTGAATATTTAAAAATTTTCAATATAAAAAGCCAGAGGCCTGGGCA; GGGACAGGCCCAAAGATGTCTCTGCCTGAGAACTAAGTGATGGGGCAAACCCACTTAATA; GTGGCCAGAGAGCAAAGGAGAGTTATAAGAAACCGTAAACCAGGCTAGGGCAGATTCACC; TTCCTAGGGGCAAGACAAAGAAGGAAGGGGGTAGACAGAGCCTACTAAGTAAGCTGCTTA; TCCCTTCTGCCACATGGTTCAGATTCAATCTAAGAATGTGTATGGTGACACCTAGTCAGA; GACAGGCCCTGGCAGGGGACATAAAAAACAAATAAGGCTTCACCCTTCCTCTCAAAGAGC; TTACATGCAAAGACGAAGGACCAACCAGGTACAGACCAGATGCACAGTTACTGAGCTTAT; GCTTTGATAAATGCTTAGCCAGCAGCATCAATGAACACTATGAAAGCCCAAAAAGAGAGG; CAGTTGAATCTTTCCTCAGGCCATCCAGCATTATTAGTTAAAATCTGATTTCATCCTTGG; NGGATGCATACATGATCAGAAGGGCCAAGCAAGCAGAGAGACCATGGTTTTCTCACCTGG; CTTCCTGAGTCAGGCCAAGTGCAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_000785
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000785.3, NP_000776.1
• RefSeq Size: 2503 bp
• RefSeq ORF: 1527 bp
• Locus ID: 1594
• UniProt ID: O15528
• Cytogenetics: 12q14.1
• Protein Families: Druggable Genome, P450
• Protein Pathways: Metabolic pathways, Steroid biosynthesis
• Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I. [provided by RefSeq, Jul 2008]