AFG1L (NM_145315) Human Untagged Clone

SKU SC123228

LACE1 (untagged)-Human lactation elevated 1 (LACE1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: AFG1L
• Synonyms: AFG1; c222389; LACE1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_145315 edited; CGGAAGGACGGGAGGGCACGGGAGTGCAGCCCGCCCATGTGGCTACTGGAGGTCACGTTC; CCTAACTGATCCCTTGGTTCTCTCGGGTGGAGCCTTCAGCGTGCACGGCGGGGTTTGACT; TTGCCACCGTCTCTCTTCTGGGTTCCAATAAAGTTTTCCTCTTCCTCTCCTCGTACGGAG; TTCAAGATGGCGGCCTCCTGGTCGCTCTTGGTTACCCTGCGCCCCTTAGCACAGAGCCCG; CTGAGAGGGAGATGTGTTGGGTGCGGGGCCTGGGCCGCCGCTCTCGCTCCTCTGGCCACC; GCCCCTGGGAAGCCCTTTTGGAAAGCCTATACGGTTCAGACATCCGAGAGCATGACCCCA; ACTGCCACTTCAGAGACTTATTTGAAAGCTTTGGCCGTTTGCCATGGACCTCTGGACCAC; TATGATTTTCTGATCAAAGCTCATGAGCTAAAGGATGATGAACATCAAAGAAGAGTCATA; CAGTGTTTGCAGAAATTACACGAGGACCTTAAAGGATACAATATAGAGGCAGAAGGCCTT; TTTTCAAAGCTTTTTTCAAGGAGCAAACCTCCAAGGGGCCTGTATGTTTATGGAGATGTT; GGTACAGGAAAAACAATGGTGATGGACATGTTTTATGCTTATGTGGAAATGAAGAGGAAA; AAACGGGTTCATTTTCATGGTTTCATGCTAGATGTGCACAAAAGAATACATCGCCTTAAA; CAGAGTTTGCCAAAAAGGAAACCAGGATTCATGGCTAAATCATATGACCCAATAGCTCCC; ATAGCCGAAGAAATCAGCGAAGAAGCATGTCTCCTATGTTTTGATGAATTTCAGGTCACT; GACATTGCTGATGCCATGATTCTGAAACAGCTTTTTGAAAATCTGTTCAAAAACGGGGTC; GTCGTTGTGGCAACATCCAACAGGCCACCGGAAGATCTCTATAAAAATGGACTCCAAAGA; GCTAACTTTGTACCATTCATAGCAGTCTTGAAGGAATATTGTAATACAGTCCAGCTAGAT; TCTGGGATAGATTACCGGAAAAGGGAACTTCCTGCTGCAGGAAAACTCTACTACCTCACA; AGTGAAGCTGATGTGGAGGCTGTCATGGATAAGTTGTTTGATGAGCTGGCTCAGAAACAA; AATGATTTAACTAGACCAAGGATTCTAAAAGTGCAAGGCAGAGAGCTGCGCCTGAATAAA; GCCTGTGGAACCGTTGCCGACTGCACATTTGAAGAGCTGTGTGAGAGACCACTTGGAGCC; AGTGACTATTTGGAACTATCAAAGAATTTTGATACAATATTTTTACGAAACATTCCGCAA; TTTACTCTGGCAAACAGGACTCAAGGTCGAAGATTCATAACTCTCATCGATAACTTTTAT; GATCTCAAGGTGCGTATAATTTGCTCTGCGTCGACTCCTATATCAAGCTTATTTTTGCAT; CAACATCATGACAGTGAGTTGGAGCAAAGCAGAATACTGATGGATGATTTGGGGCTGAGC; CAGGATTCAGCAGAAGGACTCTCCATGTTTACCGGAGAAGAGGAAATCTTTGCATTTCAG; CGCACAATTTCCCGACTCACGGAAATGCAGACTGAACAGTACTGGAATGAAGGAGACAGA; ACCAAGAAGTAACTGCCACTTTTGCATAAATAAAACTCTAGACAAATGGTTAACGCAGGC; AGAACTCCTTATTGTGGGACTTGAAGGAATCATTTTCTCATCATTAATTATGCACTTTGT; CCTCTGGACCATTTTAATCTAAAATTGCTCTCAAGGATCTAGTGGATTAGTAAGTTAAAC; ACTTAACATTTTTTAGGTCTGCTTTTTCTTATTTGGCCTTATTTCTGCACCATGAAATTA; AAATCATCTCATTCCTGAAGATGAATGTAGGACCAGACATTTTAGCTTCACATTAAACCA; CCTGAATGAACCTTGAATGCACAAGTAAATGCAAGACGTCTGGATACCTTCAAGCTTTGG; CTGCATGGTGGTGGCATGTTAGAAATACATCTTTTATAATGGGGGAAGAATGCCCTATGT; TTCAATTCAAAACAAAGACAGTTTTCCAATCTGATATAAACTAAAAGGGATAATATTTTA; GAATATGGGCTTATATGTGGTTTTTTTTTTTTGGTTATGAATTACAGTAAATCTCCCAGG; CCTTCAGATTTCTACATAATGGAAGAATATGTTTTTAACCCTGAGATCAAGTTTAGTAGG; CAGGACTTGATCTCAAGTGGTCACAAAGTTTTCTAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_145315 unedited; NGTTTTTATTTGTATACGATCATATAGGCGGCCCGCGATTCCCGGGNAATCGTCGACCCA; CGCGTCNCGCGGAAGACGGGAGGGCACGGGAGTGCAGCCCGCCCATGTGGCTACTGGAGG; TCACGTTCCCTAACTGATCCCTTGGTTCTCTCGGGTGGAGCCTTCAGCGTGCACGGCGGG; GTTTGACTTTGCCACCGTCTCTCTTCTGGGTTCCAATAAAGTTTTCCTCTTCCTCTCCTC; GTACGGAGTTCAAGATGGCGGCCTCCTGGTCGCTCTTGGTTACCCTGCGCCCCTTAGCAC; AGAGCCCGCTGAGAGGGAGATGTGTTGGGTGCGGGGCCTGGGCCGCCGCTCTCGCTCCTC; TGGCCACCGCCCCTGGGAAGCCCTTTTGGAAAGCCTATACGGTTCAGACATCCGAGAGCA; TGACCCCAACTGCCACTTCAGAGACTTATTTGAAAGCTTTGGCCGTTTGCCATGGACCTC; TGGACCACTATGATTTTCTGATCAAAGCTCATGAGCTAAAGGATGATGAACATCAAAGAA; GAGTCATACAGTGTTTGCAGAAATTACACGAGGACCTTAAAGGATACAATATAGAGGCAG; AAGGCCTTTTTTCAAAGCTTTTTTCAAGGAGCAAACCTCCAAGGGGCCTGTATGTTTATG; GAGATGTTGGTACAGGAAAAACAATGGTGATGGACATGTTTTATGCTTATGTGGAAATGA; AGAGGAAAAAACGGGTTCATTTTCATGGTTTCATGCTAGATGTGCACAAAAGAATACATC; GCCTTANACAGAGTTTGCCAAAAAGGAAACCAGGATTCATGGCTAAATCATATGACCCAA; TAGCTCCCATAGCCGAAGNAATCAGCGAAGAAGCATGTCTCCCTATGTTTGATGAANTTC; ANGTCACTGACATTGCTGATGCCCN;
• Restriction Sites: Please inquire
• ACCN: NM_145315
• Insert Size: 2271 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_145315.3, NP_660358.2
• RefSeq Size: 2262 bp
• RefSeq ORF: 1446 bp
• Locus ID: 246269
• UniProt ID: Q8WV93
• Cytogenetics: 6q21
• Summary: This gene encodes a mitochondrial integral membrane protein that plays a role in mitochondrial protein homeostasis. The protein contains a P-loop motif and a five-domain structure that is conserved in fly, yeast, and bacteria. It functions to mediate the degradation of nuclear-encoded complex IV subunits. Two conserved estrogen receptor binding sites are located within 2.5 kb of this gene. Polymorphisms in this gene have been associated with bipolar disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2016]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.