AFG1L (NM_145315) Human Untagged Clone

CAT#: SC123228

LACE1 (untagged)-Human lactation elevated 1 (LACE1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AFG1L
• Synonyms: AFG1; c222389; LACE1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_145315 edited
  CGGAAGGACGGGAGGGCACGGGAGTGCAGCCCGCCCATGTGGCTACTGGAGGTCACGTTC
  CCTAACTGATCCCTTGGTTCTCTCGGGTGGAGCCTTCAGCGTGCACGGCGGGGTTTGACT
  TTGCCACCGTCTCTCTTCTGGGTTCCAATAAAGTTTTCCTCTTCCTCTCCTCGTACGGAG
  TTCAAGATGGCGGCCTCCTGGTCGCTCTTGGTTACCCTGCGCCCCTTAGCACAGAGCCCG
  CTGAGAGGGAGATGTGTTGGGTGCGGGGCCTGGGCCGCCGCTCTCGCTCCTCTGGCCACC
  GCCCCTGGGAAGCCCTTTTGGAAAGCCTATACGGTTCAGACATCCGAGAGCATGACCCCA
  ACTGCCACTTCAGAGACTTATTTGAAAGCTTTGGCCGTTTGCCATGGACCTCTGGACCAC
  TATGATTTTCTGATCAAAGCTCATGAGCTAAAGGATGATGAACATCAAAGAAGAGTCATA
  CAGTGTTTGCAGAAATTACACGAGGACCTTAAAGGATACAATATAGAGGCAGAAGGCCTT
  TTTTCAAAGCTTTTTTCAAGGAGCAAACCTCCAAGGGGCCTGTATGTTTATGGAGATGTT
  GGTACAGGAAAAACAATGGTGATGGACATGTTTTATGCTTATGTGGAAATGAAGAGGAAA
  AAACGGGTTCATTTTCATGGTTTCATGCTAGATGTGCACAAAAGAATACATCGCCTTAAA
  CAGAGTTTGCCAAAAAGGAAACCAGGATTCATGGCTAAATCATATGACCCAATAGCTCCC
  ATAGCCGAAGAAATCAGCGAAGAAGCATGTCTCCTATGTTTTGATGAATTTCAGGTCACT
  GACATTGCTGATGCCATGATTCTGAAACAGCTTTTTGAAAATCTGTTCAAAAACGGGGTC
  GTCGTTGTGGCAACATCCAACAGGCCACCGGAAGATCTCTATAAAAATGGACTCCAAAGA
  GCTAACTTTGTACCATTCATAGCAGTCTTGAAGGAATATTGTAATACAGTCCAGCTAGAT
  TCTGGGATAGATTACCGGAAAAGGGAACTTCCTGCTGCAGGAAAACTCTACTACCTCACA
  AGTGAAGCTGATGTGGAGGCTGTCATGGATAAGTTGTTTGATGAGCTGGCTCAGAAACAA
  AATGATTTAACTAGACCAAGGATTCTAAAAGTGCAAGGCAGAGAGCTGCGCCTGAATAAA
  GCCTGTGGAACCGTTGCCGACTGCACATTTGAAGAGCTGTGTGAGAGACCACTTGGAGCC
  AGTGACTATTTGGAACTATCAAAGAATTTTGATACAATATTTTTACGAAACATTCCGCAA
  TTTACTCTGGCAAACAGGACTCAAGGTCGAAGATTCATAACTCTCATCGATAACTTTTAT
  GATCTCAAGGTGCGTATAATTTGCTCTGCGTCGACTCCTATATCAAGCTTATTTTTGCAT
  CAACATCATGACAGTGAGTTGGAGCAAAGCAGAATACTGATGGATGATTTGGGGCTGAGC
  CAGGATTCAGCAGAAGGACTCTCCATGTTTACCGGAGAAGAGGAAATCTTTGCATTTCAG
  CGCACAATTTCCCGACTCACGGAAATGCAGACTGAACAGTACTGGAATGAAGGAGACAGA
  ACCAAGAAGTAACTGCCACTTTTGCATAAATAAAACTCTAGACAAATGGTTAACGCAGGC
  AGAACTCCTTATTGTGGGACTTGAAGGAATCATTTTCTCATCATTAATTATGCACTTTGT
  CCTCTGGACCATTTTAATCTAAAATTGCTCTCAAGGATCTAGTGGATTAGTAAGTTAAAC
  ACTTAACATTTTTTAGGTCTGCTTTTTCTTATTTGGCCTTATTTCTGCACCATGAAATTA
  AAATCATCTCATTCCTGAAGATGAATGTAGGACCAGACATTTTAGCTTCACATTAAACCA
  CCTGAATGAACCTTGAATGCACAAGTAAATGCAAGACGTCTGGATACCTTCAAGCTTTGG
  CTGCATGGTGGTGGCATGTTAGAAATACATCTTTTATAATGGGGGAAGAATGCCCTATGT
  TTCAATTCAAAACAAAGACAGTTTTCCAATCTGATATAAACTAAAAGGGATAATATTTTA
  GAATATGGGCTTATATGTGGTTTTTTTTTTTTGGTTATGAATTACAGTAAATCTCCCAGG
  CCTTCAGATTTCTACATAATGGAAGAATATGTTTTTAACCCTGAGATCAAGTTTAGTAGG
  CAGGACTTGATCTCAAGTGGTCACAAAGTTTTCTAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_145315 unedited
  NGTTTTTATTTGTATACGATCATATAGGCGGCCCGCGATTCCCGGGNAATCGTCGACCCA
  CGCGTCNCGCGGAAGACGGGAGGGCACGGGAGTGCAGCCCGCCCATGTGGCTACTGGAGG
  TCACGTTCCCTAACTGATCCCTTGGTTCTCTCGGGTGGAGCCTTCAGCGTGCACGGCGGG
  GTTTGACTTTGCCACCGTCTCTCTTCTGGGTTCCAATAAAGTTTTCCTCTTCCTCTCCTC
  GTACGGAGTTCAAGATGGCGGCCTCCTGGTCGCTCTTGGTTACCCTGCGCCCCTTAGCAC
  AGAGCCCGCTGAGAGGGAGATGTGTTGGGTGCGGGGCCTGGGCCGCCGCTCTCGCTCCTC
  TGGCCACCGCCCCTGGGAAGCCCTTTTGGAAAGCCTATACGGTTCAGACATCCGAGAGCA
  TGACCCCAACTGCCACTTCAGAGACTTATTTGAAAGCTTTGGCCGTTTGCCATGGACCTC
  TGGACCACTATGATTTTCTGATCAAAGCTCATGAGCTAAAGGATGATGAACATCAAAGAA
  GAGTCATACAGTGTTTGCAGAAATTACACGAGGACCTTAAAGGATACAATATAGAGGCAG
  AAGGCCTTTTTTCAAAGCTTTTTTCAAGGAGCAAACCTCCAAGGGGCCTGTATGTTTATG
  GAGATGTTGGTACAGGAAAAACAATGGTGATGGACATGTTTTATGCTTATGTGGAAATGA
  AGAGGAAAAAACGGGTTCATTTTCATGGTTTCATGCTAGATGTGCACAAAAGAATACATC
  GCCTTANACAGAGTTTGCCAAAAAGGAAACCAGGATTCATGGCTAAATCATATGACCCAA
  TAGCTCCCATAGCCGAAGNAATCAGCGAAGAAGCATGTCTCCCTATGTTTGATGAANTTC
  ANGTCACTGACATTGCTGATGCCCN
  
• Restriction Sites: Please inquire
• ACCN: NM_145315
• Insert Size: 2271 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145315.3, NP_660358.2
• RefSeq Size: 2262 bp
• RefSeq ORF: 1446 bp
• Locus ID: 246269
• UniProt ID: Q8WV93
• Cytogenetics: 6q21
• Gene Summary: This gene encodes a mitochondrial integral membrane protein that plays a role in mitochondrial protein homeostasis. The protein contains a P-loop motif and a five-domain structure that is conserved in fly, yeast, and bacteria. It functions to mediate the degradation of nuclear-encoded complex IV subunits. Two conserved estrogen receptor binding sites are located within 2.5 kb of this gene. Polymorphisms in this gene have been associated with bipolar disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2016]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.