MSI2 (NM_138962) Human Untagged Clone

CAT#: SC123160

MSI2 (untagged)-Human musashi homolog 2 (Drosophila) (MSI2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MSI2
• Synonyms: MSI2H
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_138962 edited
  CTCGCCGCTGCCCCGGCTCCGCCGCTCGCAGAGAGATTCGGAGGAGCCCGGGCGGGGGGG
  AGGAGGAGGGGGAGGAGGGAGCGGAGATCTCGGGGCTCGGAGCCGGCCGCCGCTCCGCTC
  CGATCGCTGTGGGGCTTGGTTTTTTGGGGGTGGGGGGGCGGGGGGGCTCAGATATGGAGG
  CAAATGGGAGCCAAGGCACCTCGGGCAGCGCCAACGACTCCCAGCACGACCCCGGTAAAA
  TGTTTATCGGTGGACTGAGCTGGCAGACCTCACCAGATAGCCTTAGAGACTATTTTAGCA
  AATTTGGAGAAATTAGAGAATGTATGGTCATGAGAGATCCCACTACGAAACGCTCCAGAG
  GCTTCGGTTTCGTCACGTTCGCAGACCCAGCAAGTGTAGATAAAGTATTAGGTCAGCCCC
  ACCATGAGTTAGATTCCAAGACGATTGACCCCAAAGTTGCATTTCCTCGTCGAGCGCAAC
  CCAAGATGGTCACGAGAACAAAGAAAATATTTGTAGGCGGGTTATCTGCGAACACAGTAG
  TGGAAGATGTAAAGCAATATTTCGAGCAGTTTGGCAAGGTGGAAGATGCAATGCTGATGT
  TTGATAAAACTACCAACAGGCACAGAGGGTTTGGCTTTGTCACTTTTGAGAATGAAGATG
  TTGTGGAGAAAGTCTGTGAGATTCATTTCCATGAAATCAATAATAAAATGGTAGAATGTA
  AGAAAGCTCAGCCGAAAGAAGTCATGTTCCCACCTGGGACAAGAGGCCGGGCCCGGGGAC
  TGCCTTACACCATGGACGCGTTCATGCTTGGCATGGGGATGCTGGGATATCCCAACTTCG
  TGGCGACCTATGGCCGTGGCTACCCCGGATTTGCTCCAAGCTATGGCTATCAGTTCCCAG
  GCTTCCCAGCAGCGGCTTATGGACCAGTGGCAGCAGCGGCGGTGGCGGCAGCAAGAGGAT
  CAGGCTCCAACCCGGCGCGGCCCGGAGGCTTCCCGGGGGCCAACAGCCCAGGACCTGTCG
  CCGATCTCTACGGCCCTGCCAGCCAGGACTCCGGAGTGGGGAATTACATAAGTGCGGCCA
  GCCCACAGCCGGGCTCGGGCTTCGGCCACGGCATAGCTGGACCTTTGATTGCAACGGCCT
  TTACAAATGGATACCATTGAGCAGGTGCTTTCGTTGCCATCTCACTCTGAGAGCATACCT
  GGATGTCCAGGCAAGACTGGGCGAAGTTTCTGAGTGGCCCTTTGTTTAGGTGATGTCCTC
  AGACCTGGACCCCCACCAGCCTCACTCCCCATCCCAACCAGAGATGGCTCACTTCGGATC
  GAGGGTTGACTACATCTCATCATCTCACGAATCTGCTGTAATATAAGACAACAGCTTTTA
  AATGTGTATATAACCCAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_138962 unedited
  NAAGTCGACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCTCGCCG
  CTGCCCCGGCTCCGCCGCTCGCATAGAGATTCGGAGGAGCCCGGGCGGGGGGGAGGAGGA
  GGGGGAGGAGGGAGCGGAGATCTCGGGGCTCGGAGCCGGCCGCCGCTCCGCTCCGATCGC
  TGTGGGGCTTGGTTTTTTGGGGGTGGGGGGGCGGGGGGGCTCATATATGGAGGCAAATGG
  GAGCCAAGGCACCTCGGGCAGCGCCAACGACTCCCAGCACGACCCCGGTAAAATGTTTAT
  CGGTGGACTGAGCTGGCAGACCTCACCAGATAGCCTTAGAGACTATTTTAGCAAATTTGG
  AGAAATTAGAGAATGTATGGTCATGAGAGATCCCACTACGAAACGCTCCAGAGGCTTCGG
  TTTCGTCACGTTCGCAGACCCAGCAAGTGTAGATAAAGTATTACGTCAGCCCCACCATGA
  GTTAGATTCCAAGACGATTGACCCCAAAGTTGCATTTCCTCGTCGAGCGCAACCCAAGAT
  GGTCACGAGAACAAAGAATATATTTGTAGGCGGGTTATCTGCGAACACAGTAGTGGAAGA
  TGTAAAGCAATATTTCGAGCAGTTTGGGCAGGTGGAAGATGCCATGCTGATGTTTGATAA
  ACTACCAACAGGCACAGAGGGTTTGGCTTTGTCACTTTTGAGAATGAAGATGCTGTGGAG
  AAAGTCTGTGAGATTCATTTCCATGAAATCAAATATAAAATGGTAGATTGCTAGAAAGCT
  CACCCGAAAGGAGTCATGTTCCCACCTGGGACCAGAGGCCGGCCCCGGGACTGCCTTAAC
  ACATGGACGCTTCATGCTTGCATGGGGTGCTGGGATATCCAACCTC
  
• Restriction Sites: Please inquire
• ACCN: NM_138962
• Insert Size: 1400 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_138962.2.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138962.2, NP_620412.1
• RefSeq Size: 1581 bp
• RefSeq ORF: 987 bp
• Locus ID: 124540
• UniProt ID: Q96DH6
• Cytogenetics: 17q22
• Gene Summary: This gene encodes an RNA-binding protein that is a member of the Musashi protein family. The encoded protein is transcriptional regulator that targets genes involved in development and cell cycle regulation. Mutations in this gene are associated with poor prognosis in certain types of cancers. This gene has also been shown to be rearranged in certain cancer cells. [provided by RefSeq, Apr 2016]
  Transcript Variant: This variant (1) represents the shorter transcript, and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.