NMNAT1 (NM_022787) Human Untagged Clone

CAT#: SC122936

NMNAT1 (untagged)-Human nicotinamide nucleotide adenylyltransferase 1 (NMNAT1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NMNAT1
• Synonyms: LCA9; NMNAT; PNAT1; SHILCA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_022787 edited
  AGAGTGCGACCGAGATGTTCCACTCGCTGGCGTCCGGGCCGCTGGTGATCTCCGGTAGCA
  CTCGGGCCGGCGGACAGTGAGGGCGCGACAACAAGGGAGGTGTCACAGTTTTCCATTTAG
  ATCAACAACTTCAAGTTCTTACCATGGAAAATTCCGAGAAGACTGAAGTGGTTCTCCTTG
  CTTGTGGTTCATTCAATCCCATCACCAACATGCACCTCAGGTTGTTTGAGCTGGCCAAGG
  ACTACATGAATGGAACAGGAAGGTACACAGTTGTCAAAGGCATCATCTCTCCTGTTGGTG
  ATGCCTACAAGAAGAAAGGACTCATTCCTGCCTATCACCGGGTCATCATGGCAGAACTTG
  CTACCAAGAATTCTAAATGGGTGGAAGTTGATACATGGGAAAGTCTTCAGAAGGAGTGGA
  AAGAGACTCTGAAGGTGCTAAGACACCATCAAGAGAAATTGGAGGCTAGTGACTGTGATC
  ACCAGCAGAACTCACCTACTCTAGAAAGGCCTGGAAGGAAGAGGAAGTGGACTGAAACAC
  AAGATTCTAGTCAAAAGAAATCCCTAGAGCCAAAAACAAAAGCTGTGCCAAAGGTCAAGC
  TGCTGTGTGGGGCAGATTTATTGGAGTCCTTTGCTGTTCCCAATTTGTGGAAGAGTGAAG
  ACATCACCCAAATCGTGGCCAACTATGGGCTCATATGTGTTACTCGGGCTGGAAATGATG
  CTCAGAAGTTTATCTATGAATCGGATGTGCTGTGGAAACACCGGAGCAACATTCACGTGG
  TGAATGAATGGATCGCTAATGACATCTCATCCACAAAAATCCGGAGAGCCCTCAGAAGGG
  GCCAGAGCATTCGCTACTTGGTACCAGATCTTGTCCAAGAATACATTGAAAAGCATAATT
  TGTACAGCTCTGAGAGTGAAGACAGGAATGCTGGGGTCATCCTGGCCCCTTTGCAGAGAA
  ACACTGCAGAAGCTAAGACATAGGAATTCTACAGCATGATATTTCAGACTTCCCATTTGG
  GGATCTGAAACAATCTGGGAGTTAATAACTGGGGAAAGAAGTTGTGATCTGTTGCCTAAA
  CTAAAGCTTAAAAGTTTAGTAAAAATCGTCTGGGCACAGTGGCTCACGCCTGTAGTCCCA
  GCTACTTGGGAGGCTGAGGCAGGAGAATCACTTGACCCCAGGTGGTGGAGGTTGCAGTGA
  GCCAAGATTGCACCATTGCACTCCAGCCTGGCGACAGAGCAAGACTCTGTCTCAAAAAAA
  AAAAAAAAATTTAGTAAAAATCAATGGTAAGCTAAAATAAGTTTTTGTTTGTTTATTTGT
  TTTTGAGATGGAGTCTCTACTAAAAATACAAAAAATTAGCCAGGCATGGTGCCGCATAAC
  TATAATCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCACAG
  GTTCCAGTGGGCCAAGGTTGTGCCACTGCACTCCAGCCTGGGCAAAAAAGCAAAACTCCA
  TCTCAAAGAGAAAAAAAAAAAAGACCGGGTGTGGTGGCTCACACCTGTAATCCCAGCACT
  TTGGGAGGCCTAAGTGGGTGGATCACGTGAGGTCAAGAGTTCAAGACCAGCCTGGCCAAT
  ATGGTGAAACCCCATCTCTACTAAGAATACAAAAAATTAGCTGAGCATGGTGGTGGGCTC
  CTGTAGTCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATCGCTTGAACCTGGGAGGCAGA
  GGTTGCAGTAAGCCAAGATCGTGCCATTGCACTCCAGCCTGGGTGACAGAGCGAGACTCC
  ATCTCAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022787 unedited
  NAGAACAGTACAGATATTTGTAAACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGN
  AGAGTGCGACCGAGNAGTTCCACTCGCTGGCGTCCGGGCCGCTGGTGATCTCCGGTAGCA
  CTCGGGCCGGCGGACAGTGAGGGCGCGACAACAAGGGAGGTGTCACAGTTTTCCATTTAG
  ATCAACAACTTCAAGTTCTTACCATGGAAAATTCCGAGAAGACTGAAGTGGTTCTCCTTG
  CTTGTGGTTCATTCAATCCCATCACCAACATGCACCTCAGGTTGTTTGAGCTGGCCAAGG
  ACTACATGAATGGAACAGGAAGGTACACAGTTGTCAAAGGCATCATCTCTCCTGTTGGTG
  ATGCCTACAAGAAGAAAGGACTCATTCCTGCCTATCACCGGGTCATCATGGCAGAACTTG
  CTACCAAGAATTCTAAATGGGTGGAAGTTGATACATGGGAAAGTCTTCAGAAGGAGTGGA
  AAGAGACTCTGAAGGTGCTAAGACACCATCAAGAGAAATTGGAGGCTAGTGACTGTGATC
  ACCAGCAGAACTCACCTACTCTAGAAAGGCCTGGAAGGAAGAGGAAGTGGACTGAAACAC
  AAGATTCTAGTCAAAAGAAATCCCTAGAGCCAAAAACAAAAGCTGTGCCAAAGGTCAAGC
  TGCTGTGTGGGGCAGATTTATTGGAGTCCTTTGCTGTTCCCAATTTGTGGAAGAGTGAAG
  ACATCACCCAAATCGTGGCCAACTATGGGCTCATATGTGTTACTCGGGCTGGAAATGATG
  CTCAGAAGTTTATCTATGAATCGGATGTGCTGTGGAAACACCGGAGCAACTTCACGTGGT
  GAATGAATGGATCGCTAATGACATCTCATCCACAAAAATCCGAAAGCCCTCA
  
• Restriction Sites: Please inquire
• ACCN: NM_022787
• Insert Size: 1825 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022787.2, NP_073624.2
• RefSeq Size: 1825 bp
• RefSeq ORF: 840 bp
• Locus ID: 64802
• UniProt ID: Q9HAN9
• Cytogenetics: 1p36.22
• Domains: CTP_transf_2
• Protein Pathways: Metabolic pathways, Nicotinate and nicotinamide metabolism
• Gene Summary: This gene encodes an enzyme which catalyzes a key step in the biosynthesis of nicotinamide adenine dinucleotide (NAD). The encoded enzyme is one of several nicotinamide nucleotide adenylyltransferases, and is specifically localized to the cell nucleus. Activity of this protein leads to the activation of a nuclear deacetylase that functions in the protection of damaged neurons. Mutations in this gene have been associated with Leber congenital amaurosis 9. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are located on chromosomes 1, 3, 4, 14, and 15. [provided by RefSeq, Jul 2014]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Variants 1 and 2 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.