APOBEC3G (NM_021822) Human Untagged Clone

SKU SC122916

APOBEC3G (untagged)-Human apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G (APOBEC3G)

Product Data

• Type: Human Untagged Clone
• Target Symbol: APOBEC3G
• Synonyms: A3G; ARCD; ARP-9; ARP9; bK150C2.7; CEM-15; CEM15; dJ494G10.1; MDS019
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_021822 edited; GTGCTCCAGACAAAGATCTTAGTCGGGACTAGCCGGCCAAGGATGAAGCCTCACTTCAGA; AACACAGTGGAGCGAATGTATCGAGACACATTCTCCTACAACTTTTATAATAGACCCATC; CTTTCTCGTCGGAATACCGTCTGGCTGTGCTACGAAGTGAAAACAAAGGGTCCCTCAAGG; CCCCCTTTGGACGCAAAGATCTTTCGAGGCCAGGTGTATTCCGAACTTAAGTACCACCCA; GAGATGAGATTCTTCCACTGGTTCAGCAAGTGGAGGAAGCTGCATCGTGACCAGGAGTAT; GAGGTCACCTGGTACATATCCTGGAGCCCCTGCACAAAGTGTACAAGGGATATGGCCACG; TTCCTGGCCGAGGACCCGAAGGTTACCCTGACCATCTTTGTTGCCCGCCTCTACTACTTC; TGGGACCCAGATTACCAGGAGGCGCTTCGCAGCCTGTGTCAGAAAAGAGACGGTCCGCGT; GCCACCATGAAGATCATGAATTATGACGAATTTCAGCACTGTTGGAGCAAGTTCGTGTAC; AGCCAAAGAGAGCTATTTGAGCCTTGGAATAATCTGCCTAAATATTATATATTACTGCAC; ATCATGCTGGGGGAGATTCTCAGACACTCGATGGATCCACCCACATTCACTTTCAACTTT; AACAATGAACCTTGGGTCAGAGGACGGCATGAGACTTACCTGTGTTATGAGGTGGAGCGC; ATGCACAATGACACCTGGGTCCTGCTGAACCAGCGCAGGGGCTTTCTATGCAACCAGGCT; CCACATAAACACGGTTTCCTTGAAGGCCGCCATGCAGAGCTGTGCTTCCTGGACGTGATT; CCCTTTTGGAAGCTGGACCTGGACCAGGACTACAGGGTTACCTGCTTCACCTCCTGGAGC; CCCTGCTTCAGCTGTGCCCAGGAAATGGCTAAATTCATTTCAAAAAACAAACACGTGAGC; CTGTGCATCTTCACTGCCCGCATCTATGATGATCAAGGAAGATGTCAGGAGGGGCTGCGC; ACCCTGGCCGAGGCTGGGGCCAAAATTTCAATAATGACATACAGTGAATTTAAGCACTGC; TGGGACACCTTTGTGGACCACCAGGGATGTCCCTTCCAGCCCTGGGATGGACTAGATGAG; CACAGCCAAGACCTGAGTGGGAGGCTGCGGGCCATTCTCCAGAATCAGGAAAACTGAAGG; ATGGGCCTCAGTCTCTAAGGAAGGCAGAGACCTGGGTTGAGCCTCAGAATAAAAGATCTT; CTTCCAAGAAATGCAAACAGGCTGTTCACCACCATCTCCAGCTGATCACAGACACCAGCA; AAGCAATGCACTCCTGACCAAGTAGATTCTTTTAAAAATTAGAGTGCATTACTTTGAATC; AAAAATTTATTTATATTTCAAGAATAAAGTACTAAGATTGTGCTCAATACACAGAAAAGT; TTCAAACCTACTAATCCAGCGACAATTTGAATCGGTTTTGTAGGTAGAGGAATAAAATGA; AATACTAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021822 unedited; AAGTTAGCATTTGTAATACGACTTTATATAGGCGGCACGCGAATTCGCACGAGGTGCTCC; ATACAAATATCTTAGTCGGGACTAGCCGGCCAAGGATGAAGCCTCACTTCAGAAACACAG; TGGAGCGAATGTATCGAGACACATTCTCCTACAACTTTTATAATAGACCCATCCTTTCTC; GTCGGAATACCGTCTGGCTGTGCTACGAAGTGAAAACAAAGGGTCCCTCAAGGCCCCCTT; TGGACGCAAAGATCTTTCGAGGCCAGGTGTATTCCGAACTTAAGTACCACCCAGAGATGA; GATTCTTCCACTGGTTCAGCAAGTGGAGGAAGCTGCATCGTGACCAGGAGTATGAGGTCA; CCTGGTACATATCCTGGAGCCCCTGCACAAAGTGTACAAGGGATATGGCCACGTTCCTGG; CCGAGGACCCGAAGGTTACCCTGACCATCTTTGTTGCCCGCCTCTACTACTTCTGGGACC; CAGATTACCAGGAGGCGCTTCGCAGCCTGTGTCAGAAAAGAGACGGTCCGCGTGCCACCA; TGAAGATCATGAATTATGACGAATTTCAGCACTGTTGGAGCAAGTTCGTGTACAGCCAAA; GAGAGCTATTTGAGCCTTGGAATAATCTGCCTAAATATTATATATTACTGCACATCATGC; TGGGGGAGATTCTCAGACACTCGATGGATCCACCCACATTCACTTTCAACTTTAACAATG; AAACCTGNNGTCAGAGACGGCATGAGACTTACCTGTNGTATGAGGTGGAGCGCATGCANC; ATGACACCTGGGTCCTGCTGAACCAGCGCAGGGGCTTTCTATGCAACAGNCTCCNACATA; ACACGGTTTCCCTTTGAAGCCGNCATGCANAGCTGGGCTTCCCTGGACGTGATTCCCNTT; TTGGAAGCTGAACCTGGNACAG;
• Restriction Sites: Please inquire
• ACCN: NM_021822
• Insert Size: 1526 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_021822.1, NP_068594.1
• RefSeq Size: 1717 bp
• RefSeq ORF: 1155 bp
• Locus ID: 60489
• UniProt ID: Q9HC16
• Cytogenetics: 22q13.1
• Summary: This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. The protein encoded by this gene catalyzes site-specific deamination of both RNA and single-stranded DNA. The encoded protein has been found to be a specific inhibitor of human immunodeficiency virus-1 (HIV-1) infectivity. [provided by RefSeq, Mar 2017]; Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.