APEX2 (NM_014481) Human Untagged Clone

SKU SC122813

APEX2 (untagged)-Human APEX nuclease (apurinic/apyrimidinic endonuclease) 2 (APEX2), nuclear gene encoding mitochondrial protein

Product Data

• Type: Human Untagged Clone
• Target Symbol: APEX2
• Synonyms: APE2; APEXL2; XTH2; ZGRF2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_014481 edited; AGGTTGGCGCGGGCTGGGAGGTGTTCCAGCCCTTTAAGATGTTGCGCGTGGTGAGCTGGA; ACATCAATGGGATTCGGAGACCCCTGCAAGGGGTGGCAAATCAGGAACCCAGCAACTGTG; CCGCCGTGGCCGTGGGGCGCATTTTGGACGAGCTGGATGCGGATATCGTCTGTCTCCAGG; AAACCAAAGTGACCAGGGATGCACTGACAGAGCCCCTGGCTATCGTTGAGGGTTATAACT; CCTATTTCAGCTTCAGCCGCAACCGTAGCGGCTATTCTGGTGTAGCCACCTTCTGTAAGG; ACAATGCTACCCCAGTGGCTGCTGAAGAAGGCCTGAGTGGCCTGTTTGCCACCCAGAATG; GGGATGTTGGTTGCTATGGAAACATGGATGAGTTTACCCAAGAGGAACTCCGGGCTCTGG; ATAGTGAGGGCAGGGCCCTCCTCACACAGCATAAGATCCGCACATGGGAAGGTAAGGAGA; AGACCTTGACCCTAATCAACGTGTACTGCCCCCATGCGGACCCTGGGAGGCCTGAGCGGC; TAGTCTTTAAGATGCGCTTCTATCGTTTGCTGCAAATCCGAGCAGAAGCCCTCCTGGCGG; CAGGCAGCCATGTGATCATTCTGGGTGACCTGAATACAGCCCACCGCCCCATTGACCACT; GGGATGCAGTCAACCTGGAATGCTTTGAAGAGGACCCAGGGCGCAAGTGGATGGACAGCT; TGCTCAGTAACTTGGGGTGCCAGTCTGCCTCTCATGTAGGGCCCTTCATCGATAGCTACC; GCTGCTTCCAACCAAAGCAGGAGGGGGCCTTCACCTGCTGGTCAGCAGTCACTGGCGCCC; GCCATCTCAACTATGGCTCCCGGCTTGACTATGTGCTGGGGGACAGGACCCTGGTCATAG; ACACCTTTCAGGCCTCTTTCCTGCTGCCTGAGGTGATGGGCTCTGACCACTGCCCTGTGG; GTGCAGTCTTGAGTGTGTCCTCTGTGCCTGCAAAACAGTGCCCACCTCTGTGCACCCGCT; TCCTCCCTGAGTTTGCAGGCACCCAGCTCAAGATCCTTCGCTTCCTAGTTCCTCTCGAAC; AAAGTCCTGTGTTGGAGCAGTCGACGCTGCAGCACAACAATCAAACCCGGGTACAGACAT; GCCAAAACAAAGCCCAAGTGCGCTCAACCAGGCCTCAGCCCAGTCAGGTTGGCTCTAGCA; GAGGCCAGAAAAACCTGAAGAGCTACTTTCAGCCCTCCCCTAGCTGTCCCCAAGCCTCTC; CTGACATAGAGCTGCCTAGCCTACCACTGATGAGCGCCCTCATGACCCCGAAGACTCCAG; AAGAGAAGGCAGTGGCCAAAGTGGTGAAGGGGCAGGCCAAGACTTCAGAAGCCAAAGATG; AGAAGGAGTTACGGACCTCATTCTGGAAGTCTGTGCTGGCGGGGCCCTTGCGCACACCCC; TCTGTGGGGGCCACAGGGAGCCATGTGTGATGCGTACTGTGAAGAAGCCAGGACCCAACT; TGGGCCGCCGCTTCTACATGTGTGCCAGGCCCCGGGGTCCTCCCACTGACCCCTCCTCCC; GGTGCAACTTCTTCCTCTGGAGCAGGCCCAGCTGAACCAATGGAGGCCTGGGGACATCTG; GCATGGTCACCCCTGCACATGATCTGAGGCCAGCTCCCCTTCCCTGAGCTGCCTCCTGCT; TCTCCCTCAAAGTCTCCTACCCTTCTCTTCCTCTTTTAAGCCCTCTCTTCCTCGCTTTCC; TTCCTACCTAGCTCCTTGTTGGTGAGCTTCTTGTGCCTTAATCCTGTGACCCAGCCCCTT; ACACCACTTTCCACCTTCCTGTCCGAAGTACACGGACACTAGCTGCCCCAGGAAGTTGTG; TGATTTTAAATCACTTCTGTCTTTGCTGGAAAGTGTATTTGTGCATAAATAAAGTCTGTG; TATTTGTTTCAAAAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014481 unedited; NAACGTCGAAATTGTAAACGCCTCCTATAGGCGGCCGCGACATTCGCACGAGGAGGTTGG; CGCGGGCTGGGAGGTGTTCCAGCCCTTTAAGAGTTGCGCGTGGTGAGCTGGAACATCAAT; GGGATTCGGAGACCCCTGCAAGGGGTGGCAAATCAGGAACCCAGCAACTGTGCCGCCGTG; GCCGTGGGGCGCATTTTGGACGAGCTGGATGCGGATATCGTCTGTCTCCAGGAAACCAAA; GTGACCAGGGATGCACTGACAGAGCCCCTGGCTATCGTTGAGGGTTATAACTCCTATTTC; AGCTTCAGCCGCAACCGTAGCGGCTATTCTGGTGTAGCCACCTTCTGTAAGGACAATGCT; ACCCCAGTGGCTGCTGAAGAAGGCCTGAGTGGCCTGTTTGCCACCCAGAATGGGGATGTT; GGTTGCTATGGAAACATGGATGAGTTTACCCAAGAGGAACTCCGGGCTCTGGATAGTGAG; GGCAGGGCCCTCCTCACACAGCATAAGATCCGCACATGGGAAGGTAAGGAGAAGACCTTG; ACCCTAATCAACGTGTACTGCCCCCATGCGGACCCTGGGAGGCCTGAGCGGCTAGTCTTT; AAGATGCGCTTCTATCGTTTGCTGCAAATCCGAGCAGAAGCCCTCCTGGCGGCAGGCAGC; CATGTGATCATTCTGGGTGACCTGAATACAGCCCACCGCCCCATTGACCACTGGGATGCA; GTCAACCTGGAATGCTTTGAAGAGGACCCAGGGCGCAAGTGGATGGACAGCTTGCTCAGT; AACTTGGGGTGGCAGTCTGCCTCTCATGTAGGGCCCTTCATCGATAGCTACCGCTGCTTC; CAACCAAAGCAGGAGGGGGCCTTCACCTGCTGGTCAGCAGTCACTGGCGCCCGCCATCTC; ACTATGGCTCCCGGCTTGACTATGTGCTGGGGGGACAGA;
• Restriction Sites: Please inquire
• ACCN: NM_014481
• Insert Size: 1953 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014481.2, NP_055296.2
• RefSeq Size: 1991 bp
• RefSeq ORF: 1557 bp
• Locus ID: 27301
• UniProt ID: Q9UBZ4
• Cytogenetics: Xp11.21
• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair
• Summary: Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes a protein shown to have a weak class II AP endonuclease activity. Most of the encoded protein is located in the nucleus but some is also present in mitochondria. This protein may play an important role in both nuclear and mitochondrial base excision repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).