NNT1 (CLCF1) (NM_013246) Human Untagged Clone

CAT#: SC122789

CLCF1 (untagged)-Human cardiotrophin-like cytokine factor 1 (CLCF1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NNT1
• Synonyms: BSF-3; BSF3; CISS2; CLC; NNT-1; NNT1; NR6
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_013246 edited
  GCCGCGGCTCGCCCTCCCACTCCGCCAGCCTCCGGGAGAGGAGCCGCACCCGGCCGGCCC
  GGCCCCAGCCCCATGGACCTCCGAGCAGGGGACTCGTGGGGGATGTTAGCGTGCCTGTGC
  ACGGTGCTCTGGCACCTCCCTGCAGTGCCAGCTCTCAATCGCACAGGGGACCCAGGGCCT
  GGCCCCTCCATCCAGAAAACCTATGACCTCACCCGCTACCTGGAGCACCAACTCCGCAGC
  TTGGCTGGGACCTATCTGAACTACCTGGGCCCCCCTTTCAACGAGCCAGACTTCAACCCT
  CCCCGCCTGGGGGCAGAGACTCTGCCCAGGGCCACTGTTGACTTGGAGGTGTGGCGAAGC
  CTCAATGACAAACTGCGGCTGACCCAGAACTACGAGGCCTACAGCCACCTTCTGTGTTAC
  TTGCGTGGCCTCAACCGTCAGGCTGCCACTGCTGAGCTGCGCCGCAGCCTGGCCCACTTC
  TGCACCAGCCTCCAGGGCCTGCTGGGCAGCATTGCGGGCGTCATGGCAGCTCTGGGCTAC
  CCACTGCCCCAGCCGCTGCCTGGGACTGAACCCACTTGGACTCCTGGCCCTGCCCACAGT
  GACTTCCTCCAGAAGATGGACGACTTCTGGCTGCTGAAGGAGCTGCAGACCTGGCTGTGG
  CGCTCGGCCAAGGACTTCAACCGGCTCAAGAAGAAGATGCAGCCTCCAGCAGCTGCAGTC
  ACCCTGCACCTGGGGGCTCATGGCTTCTGACTTCTGACCTTCTCCTCTTCGCTCCCCCTT
  CAAACCCTGCTCCCACTTTGTGAGAGCCAGCCCTGTATGCCAACACCTGTTGAGCCAGGA
  GACAGAAGCTGTGAGCCTCTGGCCCTTTCCTGGACCGGCTGGGCGTGTGATGCGATCAGC
  CCTGTCTCCTCCCCACCTCCCAAAGGTCTACCGAGCTGGGGAGGAGGTACAGTAGGCCCT
  GTCCTGTCCTGTTTCTACAGGAAGTCATGCTCGAGGGAGTGTGAAGTGGTTCAGGTTGGT
  GCAGAGGCGCTCATGGCCTCCTGCTTCTTGCCTACCACTTGGCCAGTGCCCACCCAGCCC
  CTCAGGTGGCACATCTGGAGGGCAGGGGTTGAGGGGCCACCACCACACATGCCTTTCTGG
  GGTGAAGCCCTTTGGCTGCCCCACTCTCCTTGGATGGGTGTTGCTCCCTTATCCCCAAAT
  CACTCTATACATCCAATTCAGGAAACAAACATGGTGGCAATTCTACACAAAAAGAGATGA
  GATTAACAGTGCAGGGTTGGGGTCTGCATTGGAGGTGCCCTATAAACCAGAAGAGAAAAT
  ACTGAAAGCACAGGGGCAGGGACAGACCAGACCAGACCCAGGAGTCTCCAAAGCACAGAG
  TGGCAAACAAAACCCGAGCTGAGCATCAGGACCTTGCCTCGAATTGTCTTCCAGTATTAC
  GGTGCCTCTTCTCTGCCCCCTTTCCCAGGGTATCTGTGGGTTGCCAGGCTGGGGAGGGCA
  ACCATAGCCACACCACAGGATTTCCTGAAAGTTTACAATGCAGTAGCATTTTGGGGTGTA
  GGGTGGCAGCTCCCCAAGGCCCTGCCCCCCAGCCCCACCCACTCATGACTCTAAGTGTGT
  TGTATTAATATTTATTTATTTGGAGATGTTATTTATTAGATGATATTTATTGCAGAATTT
  CTATTCTTGTATTAACAAATAAAATGCTTGCCCCAGAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013246 unedited
  NGAGCTTGGCATTTGTAATACGACTTCCTATAGGGCGGCCGCGACTTCCCGGGGTATCGT
  CGACCCACGCGTCCGGCCGCGGCTCGCCCTCCCACTCCGCCAGCCTCCGGGAGAGGAGCC
  GCACCCGGCCGGCCCGGCCCCAGCCCCATGGACCTCCGAGCAGGGGACTCGTGGGGGATG
  TTAGCGTGCCTGTGCACGGTGCTCTGGCACCTCCCTGCAGTGCCAGCTCTCAATCGCACA
  GGGGACCCAGGGCCTGGCCCCTCCATCCAGAAAACCTATGACCTCACCCGCTACCTGGAG
  CACCAACTCCGCAGCTTGGCTGGGACCTATCTGAACTACCTGGGCCCCCCTTTCAACGAG
  CCAGACTTCAACCCTCCCCGCCTGGGGGCAGAGACTCTGCCCAGGGCCACTGTTGACTTG
  GAGGTGTGGCGAAGCCTCAATGACAAACTGCGGCTGACCCAGATCTACGAGGCCTACAGC
  CACCTTCTGTGTTACTTGCGTGGCCTCAACCGTCAGGCTGCCACTGCTGAGCTGCGCCGC
  AGCCTGGCCCACGGCTGCACCAGCCTCCAGGGCCTGCTGGGCAGCATTGCGGGCGTCATG
  GCAGCTCTGGGCTAACCACTGCCCCAGCCGCTGCCTGGGACTGAACCCACTTGGACTCCT
  GGCCCTGCCACAGTGACTTCCCTCCAAAAGATGGACAACTTNCTGGCTGCTAGAGGGAGC
  TGCAAGACCTGGCCTGTGGGCGCTCGGGCCAAGGGATTTCACCCGGCTCAAGAAAGAATA
  TTCAGNCCTCCAACAGCTGGAGGTACCCTGTGCCCTGGGGGCCTAAGGGTTTCTGACTTC
  TGAACTTTTCCTTTTTGCCTCCCCCTTAAAACCCTGGTCCCCTTTTGGAGAAACCCACCC
  CTGGTTGGCAACCCCTGTTGGAN
  
• Restriction Sites: Please inquire
• ACCN: NM_013246
• Insert Size: 1736 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013246.2, NP_037378.1
• RefSeq Size: 1860 bp
• RefSeq ORF: 678 bp
• Locus ID: 23529
• UniProt ID: Q9UBD9
• Cytogenetics: 11q13.2
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway
• Gene Summary: This gene is a member of the glycoprotein (gp)130 cytokine family and encodes cardiotrophin-like cytokine factor 1 (CLCF1). CLCF1 forms a heterodimer complex with cytokine receptor-like factor 1 (CRLF1). This dimer competes with ciliary neurotrophic factor (CNTF) for binding to the ciliary neurotrophic factor receptor (CNTFR) complex, and activates the Jak-STAT signaling cascade. CLCF1 can be actively secreted from cells by forming a complex with soluble type I CRLF1 or soluble CNTFR. CLCF1 is a potent neurotrophic factor, B-cell stimulatory agent and neuroendocrine modulator of pituitary corticotroph function. Defects in CLCF1 cause cold-induced sweating syndrome 2 (CISS2). This syndrome is characterized by a profuse sweating after exposure to cold as well as congenital physical abnormalities of the head and spine. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2009]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Though this isoform has a predicted signal peptide at amino acids 1-27, experiments show it is retained within the cell.