VAMP5 (NM_006634) Human Untagged Clone

SKU SC122751

VAMP5 (untagged)-Human vesicle-associated membrane protein 5 (myobrevin) (VAMP5)

Product Data

• Type: Human Untagged Clone
• Target Symbol: VAMP5
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_006634 edited; ATGGCAGGAATAGAGTTGGAGCGGTGCCAGCAGCAGGCGAACGAGGTGACGGAAATTATG; CGTAACAACTTCGGCAAGGTCCTGGAGCGTGGTGTGAAGCTGGCCGAACTGCAGCAGCGT; TCAGACCAACTCCTGGATATGAGCTCAACCTTCAACAAGACTACACAGAACCTGGCCCAG; AAGAAGTGCTGGGAGAACATCCGTTACCGGATCTGCGTGGGGCTGGTGGTGGTTGGTGTC; CTGCTCATCATCCTGATTGTGCTGCTGGTCGTCTTTCTCCCTCAGAGCAGTGACAGCAGT; AGTGCCCCACGGACCCAGGATGCAGGCATTGCCTCAGGGCCTGGGAACTGA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006634 unedited; ACGCCTAGATTTGTAAACGACTTACTATAGGCGGCCGACGAATTCGCCATTACGGCCGGG; GGCTCTTCCTCCGGGGCCGCTGGCACTGCGGCCGCTCCGCAGGCAGAGAAGCCGGGAGCG; GGCGAGGCGGCGGCGGCAGCAGCGATGGCAGGAATAGAGTTGGAGCGGTGCCAGCAGCAG; GCGAACGAGGTGACGGAAATTATGCGTAACAACTTCGGCAAGGTCCTGGAGCGTGGTGTG; AAGCTGGCCGAACTGCAGCAGCGTTCAGACCAACTCCTGGATATGAGCTCAACCTTCAAC; AAGACTACACAGAACCTGGCCCAGAAGAAGTGCTGGGAGAACATCCGTTACCGGATCTGC; GTGGGGCTGGTGGTGGTTGGTGTCCTGCTCATCATCCTGATTGTGCTGCTGGTCGTCTTT; CTCCCTCAGAGCAGTGACAGCAGTAGTGCCCCACGGACCCAGGATGCAGGCATTGCCTCA; GGGCCTGGGAACTGACCCAGCTGGTCCTGAAGGAGAAGCCAAATGGCTGCACTGGCCGAT; TCTGGTCTCCAGAGGACCTTGGTGTTTGCTCTCCCTTGACCCACCCCAGTGAGTGCCAAA; GGGCAGCCCCAACATGTGCACCCCTGCATTTCCTGTCATGCCACAGACTGGCCCTTGAGG; GCAGCCTGCTGTACTGGCCATGCTGGGCCAGCCCCACCTGGAGCTCAGTAAAAACTGCTG; TTTGATAAAAAAAAAAAAAAAAAAAAAAAAAAACATGTCGGCCGCCTCGGCCCTCGAGTT; TTTTTTTTTTTTTTTTTAATAAAATCATACAAATCTTTTATTAAAGATCTACTCATACCA; TGGCTGAAATCATCTATTATGGTTGCTAGGTAGGCCTCTCTTCTTA;
• Restriction Sites: Please inquire
• ACCN: NM_006634
• Insert Size: 694 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006634.2, NP_006625.1
• RefSeq Size: 694 bp
• RefSeq ORF: 351 bp
• Locus ID: 10791
• UniProt ID: O95183
• Cytogenetics: 2p11.2
• Protein Families: Transmembrane
• Protein Pathways: SNARE interactions in vesicular transport
• Summary: Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of vesicles and cell membranes. The VAMP5 gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE superfamily. This VAMP family member may participate in vesicle trafficking events that are associated with myogenesis. [provided by RefSeq, Jul 2008]