Noggin (NOG) (NM_005450) Human Untagged Clone

CAT#: SC122718

NOG (untagged)-Human noggin (NOG)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Noggin
• Synonyms: SYM1; SYNS1; SYNS1A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >SC122718 representing NM_005450.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  CAGCGAGGAGCCGGCGCCTCCCGCGCCCCGCGGTCGCCCTGGAGTAATTTCGGATGCCCAGCCGCGGCC
  GCCTTCCCCAGTAGACCCGGGAGAGGAGTTGCGGCCAACTTGTGTGCCTTTCTTCCGCCCCGGTGGGAG
  CCGGCGCTGCGCGAAGGGCTCTCCCGGCGGCTCATGCTGCCGGCCCTGCGCCTGCCCAGCCTCGGGTGA
  GCCGCCTCCGGAGAGACGGGGGAGCGCGGCGGCGCCGCGGGCTCGGCGTGCTCTCCTCCGGGGACGCGG
  GACGAAGCAGCAGCCCCGGGCGCGCGCCAGAGGCATGGAGCGCTGCCCCAGCCTAGGGGTCACCCTCTA
  CGCCCTGGTGGTGGTCCTGGGGCTGCGGGCGACACCGGCCGGCGGCCAGCACTATCTCCACATCCGCCC
  GGCACCCAGCGACAACCTGCCCCTGGTGGACCTCATCGAACACCCAGACCCTATCTTTGACCCCAAGGA
  AAAGGATCTGAACGAGACGCTGCTGCGCTCGCTGCTCGGGGGCCACTACGACCCAGGCTTCATGGCCAC
  CTCGCCCCCCGAGGACCGGCCCGGCGGGGGCGGGGGTGCAGCTGGGGGCGCGGAGGACCTGGCGGAGCT
  GGACCAGCTGCTGCGGCAGCGGCCGTCGGGGGCCATGCCGAGCGAGATCAAAGGGCTAGAGTTCTCCGA
  GGGCTTGGCCCAGGGCAAGAAGCAGCGCCTAAGCAAGAAGCTGCGGAGGAAGTTACAGATGTGGCTGTG
  GTCGCAGACATTCTGCCCCGTGCTGTACGCGTGGAACGACCTGGGCAGCCGCTTTTGGCCGCGCTACGT
  GAAGGTGGGCAGCTGCTTCAGTAAGCGCTCGTGCTCCGTGCCCGAGGGCATGGTGTGCAAGCCGTCCAA
  GTCCGTGCACCTCACGGTGCTGCGGTGGCGCTGTCAGCGGCGCGGGGGCCAGCGCTGCGGCTGGATTCC
  CATCCAGTACCCCATCATTTCCGAGTGCAAGTGCTCGTGCTAGAACTCGGGGGCCCCCTGCCCGCACCC
  GGACACTTGATCGATCCCCACCGACGCCCCCTGCACCGCCTCCAACCAGTTCCACCACCCTCTAGCGAG
  GGTTTTCAATGAACTTTTTTTTTTTTTTTTTTTTTTTTCTGGGCTACAGAGACCTAGCTTTCTGGTTCC
  TGTAATGCACTGTTTAACTGTGTAGGAATGTATATGTGTGTGTATATACGGTCCCAGTTTTAATTTACT
  TATTAAAAGGTCAGTATTATACGTTAAAAAAAAAAAACCCAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005450 unedited
  NNGGTAAAGGTCAGACTTTGTAAACGAATCACTTAGGCGGCCGCGTAACTCGCCATTACG
  GCCGGGGCAGCGAGGAGCCGGCGCCTCCCGCGCCCCGCGGTCGCCCTGGAGTAATTTCGG
  ATGCCCAGCCGCGGCCGCCTTCCCCAGTAGACCCGGGAGAGGAGTTGCGGCCAACTTGTG
  TGCCTTTCTTCCGCCCCGGTGGGAGCCGGCGCTGCGCGAAGGGCTCTCCCGGCGGCTCAT
  GCTGCCGGCCCTGCGCCTGCCCAGCCTCGGGTGAGCCGCCTCCGGAGAGACGGGGGAGCG
  CGGCGGCGCCGCGGGCTCGGCGTGCTCTCCTCCGGGGACGCGGGACGAAGCAGCAGCCCC
  GGGCGCGCGCCAGAGGCATGGAGCGCTGCCCCAGCCTAGGGGTCACCCTCTACGCCCTGG
  TGGTGGTCCTGGGGCTGCGGGCGACACCGGCCGGCGGCCAGCACTATCTCCACATCCGCC
  CGGCACCCAGCGACAACCTGCCCCTGGTGGACCTCATCGAACACCCAGACCCTATCTTTG
  ACCCCAAGGAAAAGGATCTGAACGAGACGCTGCTGCGCTCGCTGCTCGGGGGCCACTACG
  ACCCAGGCTTCATGGCCACCTCGCCCCCCGAGGACCGGCCCGGCGGGGGCGGGGGTGCAG
  CTGGGGGCGCGGAGGACCTGGCGGAGCTGGACCAGCTGCTGCGGCAGCGGCCGTCGGGGG
  CCATGCCGAGCGAGATCAAAGGGCTAGAGTTCTCCGAGGGCTTGCCCCAGGGCAGAAGCA
  GCGCCTAAGCAAGAGCTGCGGAGGAAAGTACAGATGTGGCTGTGGTCGCAGACTTCTGCC
  CCGTGCTGTACGCCTGGAACGACCCTGGCAGCCCCTTTTGCCGCGCTACTTAAGGTGGGC
  AA
  
• Restriction Sites: EcoRI-XbaI
• ACCN: NM_005450
• Insert Size: 1307 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_005450.2.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005450.4
• RefSeq Size: 1892 bp
• RefSeq ORF: 699 bp
• Locus ID: 9241
• UniProt ID: Q13253
• Cytogenetics: 17q22
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: TGF-beta signaling pathway
• MW: 45.9 kDa
• Gene Summary: The secreted polypeptide, encoded by this gene, binds and inactivates members of the transforming growth factor-beta (TGF-beta) superfamily signaling proteins, such as bone morphogenetic protein-4 (BMP4). By diffusing through extracellular matrices more efficiently than members of the TGF-beta superfamily, this protein may have a principal role in creating morphogenic gradients. The protein appears to have pleiotropic effect, both early in development as well as in later stages. It was originally isolated from Xenopus based on its ability to restore normal dorsal-ventral body axis in embryos that had been artificially ventralized by UV treatment. The results of the mouse knockout of the ortholog suggest that it is involved in numerous developmental processes, such as neural tube fusion and joint formation. Recently, several dominant human NOG mutations in unrelated families with proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) were identified; both SYM1 and SYNS1 have multiple joint fusion as their principal feature, and map to the same region (17q22) as this gene. All of these mutations altered evolutionarily conserved amino acid residues. The amino acid sequence of this human gene is highly homologous to that of Xenopus, rat and mouse. [provided by RefSeq, Jul 2008]