Cystathionase (CTH) (NM_001902) Human Untagged Clone

CAT#: SC122611

CTH (untagged)-Human cystathionase (cystathionine gamma-lyase) (CTH), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Cystathionase
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001902 edited
  CTTTAGTGCGCTCGCCGTCGGCTCTACCTGCGTGCTTTAGCTCCTTCTCGCCTGATCCTT
  CTGTCTCTCCCAACCCCGGACACCCGGCTTCGACTGGTTATATCTTCGGTGTTCTTTTCC
  TCTCTTCTTCTTTCGCGGTTCAGCATGCAGGAAAAAGACGCCTCCTCACAAGGTTTCCTG
  CCACACTTCCAACATTTCGCCACGCAGGCGATCCATGTGGGCCAGGATCCAGAGCAATGG
  ACCTCCAGGGCTGTAGTGCCCCCCATCTCACTGTCCACCACGTTCAAGCAAGGGGCGCCT
  GGCCAGCACTCGGGTTTTGAATATAGCCGTTCTGGAAATCCCACTAGGAATTGCCTTGAA
  AAAGCAGTGGCAGCACTGGATGGGGCTAAGTACTGTTTGGCCTTTGCTTCAGGTTTAGCA
  GCCACTGTAACTATTACCCATCTTTTAAAAGCAGGAGACCAAATTATTTGTATGGATGAT
  GTGTATGGAGGTACAAACAGGTACTTCAGGCAAGTGGCATCTGAATTTGGATTAAAGATT
  TCTTTTGTTGATTGTTCCAAAATCAAATTACTAGAGGCAGCAATTACACCAGAAACCAAG
  CTTGTTTGGATCGAAACCCCCACAAACCCCACCCAGAAGGTGATTGACATTGAAGGCTGT
  GCACATATTGTCCATAAGCATGGAGACATTATTTTGGTCGTGGATAACACTTTTATGTCA
  CCATATTTCCAGCGCCCTTTGGCTCTGGGAGCTGATATTTCTATGTATTCTGCAACAAAA
  TACATGAATGGCCACAGTGATGTTGTAATGGGCCTGGTGTCTGTTAATTGTGAAAGCCTT
  CATAATAGACTTCGTTTCTTGCAAAACTCTCTTGGAGCAGTTCCATCTCCTATTGATTGT
  TACCTCTGCAATCGAGGTCTGAAGACTCTACATGTCCGAATGGAAAAGCATTTCAAAAAC
  GGAATGGCAGTTGCCCAGTTCCTGGAATCTAATCCTTGGGTAGAAAAGGTTATTTATCCT
  GGGCTGCCCTCTCATCCACAGCATGAGTTGGTGAAGCGTCAGTGTACAGGTTGTACAGGG
  ATGGTCACCTTTTATATTAAGGGCACTCTTCAGCATGCTGAGATTTTCCTCAAGAACCTA
  AAGCTATTTACTCTGGCCGAGAGCTTGGGAGGATTCGAAAGCCTTGCTGAGCTTCCGGCA
  ATCATGACTCATGCATCAGTTCTTAAGAATGACAGAGATGTCCTTGGAATTAGTGACACA
  CTGATTCGACTTTCTGTGGGCTTAGAGGATGAGGAAGACCTACTGGAAGATCTAGATCAA
  GCTTTGAAGGCAGCACACCCTCCAAGTGGAAGTCACAGCTAGTATTCCAGAGCTGCTATT
  AGAAGCTGCTTCCTGTGAAGATCAAATCTTCCTGAGTAATTAAATGGACCAACAATGAGC
  CTTTGCAAAATTTTCAAGCGGAAATTTTAAGGCACCTCATTATCTTTCATAACTGTAATT
  TTCTTAGGGATCATCTCTGTTAAAAAGTTTTCTGTATGTCATGTTATAATTACAGGTCAA
  TTCTGTTAATATCTTTTTGTTAATTTTGCTCTATGTTTGCCTCTGAAGGAGGTGAGATTT
  GTGCTACTTTGGGAGATTATGTTCTTTTTTCATGTCTAAGATTTATTTTGATCATGTTTA
  TAATATAATGGTAATTCATTTTTGATGTTTTGTGAAGAATTTAAATTTAAACGAATGTTC
  TTAAATCAAGTGTGATTTTTTTGCATATCATTGAAAAGAACATTAAAAGCAATGGTTTAC
  ACTTAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001902 unedited
  GGCAGTTCNGATTTGTATACGACTCATATAGGGCGGCCGCGATTCCCGGGATATCGTCGA
  CCCACGCGTCCGCCCACGCGTCCGCTTTAGTGCGCTCGCCGTCGGCTCTACCTGCGTGCT
  TTAGCTCCTTCTCGCCTGATCCTTCTGTCTCTCCCAACCCCGGACACCCGGCTTCGACTG
  GTTATATCTTCGGTGTTCTTTTCCTCTCTTCTTCTTTCGCGGTTCAGCATGCAGGAAAAA
  GACGCCTCCTCACAAGGTTTCCTGCCACACTTCCAACATTTCGCCACGCAGGCGATCCAT
  GTGGGCCAGGATCCAGAGCAATGGACCTCCAGGGCTGTAGTGCCCCCCATCTCACTGTCC
  ACCACGTTCAAGCAAGGGGCGCCTGGCCAGCACTCGGGTTTTGAATATAGCCGTTCTGGA
  AATCCCACTAGGAATTGCCTTGAAAAAGCAGTGGCAGCACTGGATGGGGCTAAGTACTGT
  TTGGCCTTTGCTTCAGGTTTAGCAGCCACTGTAACTATTACCCATCTTTTAAAAGCAGGA
  GACCAAATTATTTGTATGGATGATGTGTATGGAGGTACAAACAGGTACTTCAGGCAAGTG
  GCATCTGAATTTGGATTAAAGATTTCTTTTGTTGATTGTTCCAAAATCAAATTACTAGAG
  GCAGCAATTACACCAGANACCAAGCTTGTTTGGATCGAAACCCCCACAAACCCCACCCAG
  AAGGTGATTGACATTGAAGGCTGTGCACATATTGTCCATAAGCATGGAGACATTATTTTT
  GGTCGTGGATAACACTTTTATGTCACCATATTTCCAGCGCCCTTTGGCTCTGGGAGCTGA
  TATTTCTATGTATTCTGCNACAAATACATGAATGGCCACAGTGATGTTGTAATGGGCCTG
  
• Restriction Sites: Please inquire
• ACCN: NM_001902
• Insert Size: 1831 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001902.4, NP_001893.2
• RefSeq Size: 1819 bp
• RefSeq ORF: 1218 bp
• Locus ID: 1491
• UniProt ID: P32929
• Cytogenetics: 1p31.1
• Protein Pathways: Cysteine and methionine metabolism, Glycine, serine and threonine metabolism, Metabolic pathways, Nitrogen metabolism, Selenoamino acid metabolism
• Gene Summary: This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010]
  Transcript Variant: This variant (1) encodes the longest isoform (1) of this protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.