Amphiregulin (AREG) (NM_001657) Human Untagged Clone

SKU SC122605

AREG (untagged)-Human amphiregulin (AREG)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Amphiregulin
• Synonyms: AR; AREGB; CRDGF; SDGF
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001657 edited; GGGGAGACGTTCGCACACCTGGGTGCCAGCGCCCCAGAGGTCCCGGGACAGCCCGAGGCG; CCGCGCCCGCCGCCCCGAGCTCCCCAAGCCTTCGAGAGCGGCGCACACTCCCGGTCTCCA; CTCGCTCTTCCAACACCCGCTCGTTTTGGCGGCAGCTCGTGTCCCAGAGACCGAGTTGCC; CCAGAGACCGAGACGCCGCCGCTGCGAAGGACCAATGAGAGCCCCGCTGCTACCGCCGGC; GCCGGTGGTGCTGTCGCTCTTGATACTCGGCTCAGGCCATTATGCTGCTGGATTGGACCT; CAATGACACCTACTCTGGGAAGCGTGAACCATTTTCTGGGGACCACAGTGCTGATGGATT; TGAGGTTACCTCAAGAAGTGAGATGTCTTCAGGGAGTGAGATTTCCCCTGTGAGTGAAAT; GCCTTCTAGTAGTGAACCGTCCTCGGGAGCCGACTATGACTACTCAGAAGAGTATGATAA; CGAACCACAAATACCTGGCTATATTGTCGATGATTCAGTCAGAGTTGAACAGGTAGTTAA; GCCCCCCCAAAACAAGACGGAAAGTGAAAATACTTCAGATAAACCCAAAAGAAAGAAAAA; GGGAGGCAAAAATGGAAAAAATAGAAGAAACAGAAAGAAGAAAAATCCATGTAATGCAGA; ATTTCAAAATTTCTGCATTCACGGAGAATGCAAATATATAGAGCACCTGGAAGCAGTAAC; ATGCAAATGTCAGCAAGAATATTTCGGTGAACGGTGTGGGGAAAAGTCCATGAAAACTCA; CAGCATGATTGACAGTAGTTTATCAAAAATTGCATTAGCAGCCATAGCTGCCTTTATGTC; TGCTGTGATCCTCACAGCTGTTGCTGTTATTACAGTCCAGCTTAGAAGACAATACGTCAG; GAAATATGAAGGAGAAGCTGAGGAACGAAAGAAACTTCGACAAGAGAATGGAAATGTACA; TGCTATAGCATAACTGAAGATAAAATTACAGGATATCACATTGGAGTCACTGCCAAGTCA; TAGCCATAAATGATGAGTCGGTCCTCTTTCCAGTGGATCATAAGACAATGGACCCTTTTT; GTTATGATGGTTTTAAACTTTCAATTGTCACTTTTTATGCTATTTCTGTATATAAAGGTG; CACGAAGGTAAAAAGTATTTTTTCAAGTTGTAAATAATTTATTTAATATTTAATGGAAGT; GTATTTATTTTACAGCTCATTAAACTTTTTTAACCAAACAGATCAAAAAAAAAAAAAAAA; AAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001657 unedited; CCGCAAGTTCGGGATTTGNTATACGACTTCACTATAGGGCGGCGCGCGATTCGGCCATTA; CGGCCGGGGAGACGTTCGCACACCTGGGTGCCAGCGCCCCAGAGTCCCGGGACAGCCCGA; GGCGCCGCGCCCGCCGCCCCGAGCTCCCCAAGCCTTCGAGAGCGGCGCACACTCCCGGTC; TCCACTCGCTCTTCCAACACCCGCTCGTTTTGGCGGCAGCTCGTGTCCCAGAGACCGAGT; TGCCCCAGAGACCGAGACGCCGCCGCTGCGAAGGACCAATGAGAGCCCCGCTGCTACCGC; CGGCGCCGGTGGTGCTGTCGCTCTTGATACTCGGCTCAGGCCATTATGCTGCTGGATTGG; ACCTCAATGACACCTACTCTGGGAAGCGTGAACCATTTTCTGGGGACCACAGTGCTGATG; GATTTGAGGTTACCTCAAGAAGTGAGATGTCTTCAGGGAGTGAGATTTCCCCTGTGAATG; AAATGCCTTCTAGTAGTGAACCGTCCTCGGGAGCCGACTATGACTACTCAGAAAAGTATG; ATAACGAACCACAAATACCTGGCTATATTGGACATGATTCAGTCATAATTGAACAGGTTG; TTAAGCCCCCCCAAAACAAGGCCGGGAGGTGAAAATACTTCAGATAAACCCAACAAAAAG; AAAAGGGGGGCCAATATGGCGCGAAATGGCGGAAACCGTAGGAAGAAAAATCCCTGGAGG; GCGGAAATTGAAGATTTCTGGCTTCCCGGGGAATGCCAATATTTTGGGCCCCGGGGAGAG; GGACCTTGCCGAGTGGGGCAGAAAAATTTCGGGGAGCGGGGTGGGGAAAAGTCCCACAGA; ACTCCAACCAGGTTGGACGGTGTTTGACCAGAAATTGGTTGAGCGAGCCTAGGCTGCCTT; GAGGGCGCN;
• Restriction Sites: Please inquire
• ACCN: NM_001657
• Insert Size: 1300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001657.2, NP_001648.1
• RefSeq Size: 1270 bp
• RefSeq ORF: 759 bp
• Locus ID: 374
• UniProt ID: P15514
• Cytogenetics: 4q13.3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ErbB signaling pathway
• Summary: The protein encoded by this gene is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). The protein interacts with the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells, and it inhibits the growth of certain aggressive carcinoma cell lines. It also functions in mammary gland, oocyte and bone tissue development. This gene is associated with a psoriasis-like skin phenotype, and is also associated with other pathological disorders, including various types of cancers and inflammatory conditions. [provided by RefSeq, Apr 2014]