alpha 1 Fetoprotein (AFP) (NM_001134) Human Untagged Clone

SKU SC122582

AFP (untagged)-Human alpha-fetoprotein (AFP)

Product Data

• Type: Human Untagged Clone
• Target Symbol: alpha 1 Fetoprotein
• Synonyms: AFPD; FETA; HPAFP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001134 edited; CCACTGCCAATAACAAAATAACTAGCAACCATGAAGTGGGTGGAATCAATTTTTTTAATT; TTCCTACTAAATTTTACTGAATCCAGAACACTGCATAGAAATGAATATGGAATAGCTTCC; ATATTGGATTCTTACCAATGTACTGCAGAGATAAGTTTAGCTGACCTGGCTACCATATTT; TTTGCCCAGTTTGTTCAAGAAGCCACTTACAAGGAAGTAAGCAAAATGGTGAAAGATGCA; TTGACTGCAATTGAGAAACCCACTGGAGATGAACAGTCTTCAGGGTGTTTAGAAAACCAG; CTACCTGCCTTTCTGGAAGAACTTTGCCATGAGAAAGAAATTTTGGAGAAGTACGGACAT; TCAGACTGCTGCAGCCAAAGTGAAGAGGGAAGACATAACTGTTTTCTTGCACACAAAAAG; CCCACTCCAGCATCGATCCCACTTTTCCAAGTTCCAGAACCTGTCACAAGCTGTGAAGCA; TATGAAGAAGACAGGGAGACATTCATGAACAAATTCATTTATGAGATAGCAAGAAGGCAT; CCCTTCCTGTATGCACCTACAATTCTTCTTTGGGCTGCTCGCTATGACAAAATAATTCCA; TCTTGCTGCAAAGCTGAAAATGCAGTTGAATGCTTCCAAACAAAGGCAGCAACAGTTACA; AAAGAATTAAGAGAAAGCAGCTTGTTAAATCAACATGCATGTGCAGTAATGAAAAATTTT; GGGACCCGAACTTTCCAAGCCATAACTGTTACTAAACTGAGTCAGAAGTTTACCAAAGTT; AATTTTACTGAAATCCAGAAACTAGTCCTGGATGTGGCCCATGTACATGAGCACTGTTGC; AGAGGAGATGTGCTGGATTGTCTGCAGGATGGGGAAAAAATCATGTCCTACATATGTTCT; CAACAAGACACTCTGTCAAACAAAATAACAGAATGCTGCAAACTGACCACGCTGGAACGT; GGTCAATGTATAATTCATGCAGAAAATGATGAAAAACCTGAAGGTCTATCTCCAAATCTA; AACAGGTTTTTAGGAGATAGAGATTTTAACCAATTTTCTTCAGGGGAAAAAAATATCTTC; TTGGCAAGTTTTGTTCATGAATATTCAAGAAGACATCCTCAGCTTGCTGTCTCAGTAATT; CTAAGAGTTGCTAAAGGATACCAGGAGTTATTGGAGAAGTGTTTCCAGACTGAAAACCCT; CTTGAATGCCAAGATAAAGGAGAAGAAGAATTACAGAAATACATCCAGGAGAGCCAAGCA; TTGGCAAAGCGAAGCTGCGGCCTCTTCCAGAAACTAGGAGAATATTACTTACAAAATGCG; TTTCTCGTTGCTTACACAAAGAAAGCCCCCCAGCTGACCTCGTCGGAGCTGATGGCCATC; ACCAGAAAAATGGCAGCCACAGCAGCCACTTGTTGCCAACTCAGTGAGGACAAACTATTG; GCCTGTGGCGAGGGAGCGGCTGACATTATTATCGGACACTTATGTATCAGACATGAAATG; ACTCCAGTAAACCCTGGTGTTGGCCAGTGCTGCACTTCTTCATATGCCAACAGGAGGCCA; TGCTTCAGCAGCTTGGTGGTGGATGAAACATATGTCCCTCCTGCATTCTCTGATGACAAG; TTCATTTTCCATAAGGATCTGTGCCAAGCTCAGGGTGTAGCGCTGCAAACGATGAAGCAA; GAGTTTCTCATTAACCTTGTGAAGCAAAAGCCACAAATAACAGAGGAACAACTTGAGGCT; GTCATTGCAGATTTCTCAGGCCTGTTGGAGAAATGCTGCCAAGGCCAGGAACAGGAAGTC; TGCTTTGCTGAAGAGGGACAAAAACTGATTTCAAAAACTCGTGCTGCTTTGGGAGTTTAA; ATTACTTCAGGGGAAGAGAAGACAAAACGAGTCTTTCATTCGGTGTGAACTTTTCTCTTT; AATTTTAACTGATTTAACACTTTTTGTGAATTAATGAAATGATAAAGACTTTTATGTGAG; ATTTCCTTATCACAGAAATAAAATATCTCCAAATGTTAAAAAAAAAAAAAAAAAAAAAAA; AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001134 unedited; NGGTAACGTCAATATTTGTATACGACTCACTATAGGCGGCCGCGNATTCCCGGGATCCAC; TGCCAATAACAAAATAACTAGCAACCATGAAGTGGGTGGAATCAATTTTTTTAATTTTCC; TACTAAATTTTACTGAATCCAGAACACTGCATAGAAATGAATATGGAATAGCTTCCATAT; TGGATTCTTACCAATGTACTGCAGAGATAAGTTTAGCTGACCTGGCTACCATATTTTTTG; CCCAGTTTGTTCAAGAAGCCACTTACAAGGAAGTAAGCAAAATGGTGAAAGATGCATTGA; CTGCAATTGAGAAACCCACTGGAGATGAACAGTCTTCAGGGTGTTTAGAAAACCAGCTAC; CTGCCTTTCTGGAAGAACTTTGCCATGAGAAAGAAATTTTGGAGAAGTACGGACATTCAG; ACTGCTGCAGCCAAAGTGAAGAGGGAAGACATAACTGTTTTCTTGCACACAAAAAGCCCA; CTCCAGCATCGATCCCACTTTTCCAAGTTCCAGAACCTGTCACAAGCTGTGAAGCATATG; AAGAAGACAGGGAGACATTCATGAACAAATTCATTTATGAGATAGCAAGAAGGCATCCCT; TCCTGTATGCACCTACAATTCTTCTTTGGGCTGCTCGCTATGACAAAATAATTCCATCTT; GCTGCAAAGCTGAANATGCAGTTGAATGCCTTCCAACAAGAGCAGCAACAGTTACAAAAG; ATTTAAGAGAAGCAGCTTGTTAAATCACATGCATGTGCAGTAATGAAAAAATTTGGGACC; CGAACTTTCCAGCCTTACTGTTACTAAACTGAATCAGAGTTTACCACAGTTAATTTACTG; AATCCAAAACTAGTCCGGGAGTGGCCATGTTCTGAACACG;
• Restriction Sites: Please inquire
• ACCN: NM_001134
• Insert Size: 2098 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001134.1, NP_001125.1
• RefSeq Size: 2032 bp
• RefSeq ORF: 1830 bp
• Locus ID: 174
• UniProt ID: P02771
• Cytogenetics: 4q13.3
• Protein Families: Druggable Genome, Secreted Protein
• Summary: This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatocarcinoma and with teratoma, and has prognostic value for managing advanced gastric cancer. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Oct 2019]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).