CACNB3 (NM_000725) Human Untagged Clone

SKU SC122090

CACNB3 (untagged)-Human calcium channel, voltage-dependent, beta 3 subunit (CACNB3), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: CACNB3
• Synonyms: CAB3; CACNLB3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000725 edited; GTCGAGAGCGGGTGGGGGGAGCGGTGATCTGAGCTCCGAGCAGCTGGTCTTCGCGGCTCG; CTCCCTCCTTCGCGCTCTCTCGCTCCCTGCCGCCGCCCGCAGGGCTGCGGGGCTCGGTGG; CATCTCCCGGGCGCGGCCCGCAGTCCTTGCCCCTGCCTCCGGGCCGCTCCCGCCCCCGGC; GCCGCTCGCTCCCCCGACCCGGACTCCCCCATGTATGACGACTCCTACGTGCCCGGGTTT; GAGGACTCGGAGGCGGGTTCAGCCGACTCCTACACCAGCCGCCCATCTCTGGACTCAGAC; GTCTCCCTGGAGGAGGACCGGGAGAGTGCCCGGCGTGAAGTAGAGAGCCAGGCTCAGCAG; CAGCTCGAAAGGGCCAAGCACAAACCTGTGGCATTTGCGGTGAGGACCAATGTCAGCTAC; TGTGGCGTACTGGATGAGGAGTGCCCAGTCCAGGGCTCTGGAGTCAACTTTGAGGCCAAA; GATTTTCTGCACATTAAAGAGAAGTACAGCAATGACTGGTGGATCGGGCGGCTAGTGAAA; GAGGGCGGGGACATCGCCTTCATCCCCAGCCCCCAGCGCCTGGAGAGCATCCGGCTCAAA; CAGGAGCAGAAGGCCAGGAGATCTGGGAACCCTTCCAGCCTGAGTGACATTGGCAACCGA; CGCTCCCCTCCGCCATCTCTAGCCAAGCAGAAGCAAAAGCAGGCGGAACATGTTCCCCCA; TATGACGTGGTGCCCTCCATGCGGCCTGTGGTGCTGGTGGGACCATCTCTGAAAGGTTAT; GAGGTCACAGACATGATGCAGAAGGCTCTCTTCGACTTCCTCAAACACAGATTTGATGGC; AGGATCTCCATCACCCGAGTCACAGCCGACCTCTCCCTGGCAAAGCGATCTGTGCTCAAC; AATCCGGGCAAGAGGACCATCATTGAGCGCTCCTCTGCCCGCTCCAGCATTGCGGAAGTG; CAGAGTGAGATCGAGCGCATATTTGAGCTGGCCAAATCCCTGCAGCTAGTAGTGTTGGAC; GCTGACACCATCAACCACCCAGCACAGCTGGCCAAGACCTCGCTGGCCCCCATCATCGTC; TTTGTCAAAGTGTCCTCACCAAAGGTACTCCAGCGTCTCATTCGCTCCCGGGGGAAGTCA; CAGATGAAGCACCTGACCGTACAGATGATGGCATATGATAAGCTGGTTCAGTGCCCACCG; GAGTCATTTGATGTGATTCTGGATGAGAACCAGCTGGAGGATGCCTGTGAGCACCTGGCT; GAGTACCTGGAGGTTTACTGGCGGGCCACGCACCACCCAGCCCCTGGCCCCGGACTTCTG; GGTCCTCCCAGTGCCATCCCGGGACTTCAGAACCAGCAGCTGCTGGGGGAGCGTGGCGAG; GAGCACTCCCCCCTTGAGCGGGACAGCTTGATGCCCTCTGATGAGGCCAGCGAGAGCTCC; CGCCAAGCCTGGACAGGATCTTCACAGCGTAGCTCCCGCCACCTGGAGGAGGACTATGCA; GATGCCTACCAGGACCTGTACCAGCCTCACCGCCAACACACCTCGGGGCTGCCTAGTGCT; AACGGGCATGACCCCCAAGACCGGCTTCTAGCCCAGGACTCAGAGCACAACCACAGTGAC; CGGAACTGGCAGCGCAACCGGCCTTGGCCCAAGGATAGCTACTGACAGCCTCCTGCTGCC; CTACCCTGGCAGGCACAGGCGCAGCTGGCTGGGGGGCCCACTCCAGGCAGGGTGGCGTTA; GACTGGCATCAGGCTGGCACTAGGCTCAGCCCCCAAAACCCCCTGCCCAGCCCCAGCTTC; AGGGCTGCCTGTGGTCCCAAGGTTCTGGGAGAAACAGGGGACCCCCTCACCTCCTGGGCA; GTGACCCCTACTAGGCTCCCATTCCAGGTACTAGCTGTGTGTTCTGCACCCCTGGCACCT; TCCTCTCCTCCCACACAGGAAGCTGCCCCACTGGGCAGTGCCCTCAGGCCAGGATCCCCT; TAGCAGGGTCCTTCCCACCAGACTCAGGGAAGGGATGCCCCATTAAAGTGACAAAAGGGT; GGGGTGTGGGCACCATGGCATGAGGAAGAAACAAGGTCCCTGAGCAGGCACAAGTCCTGA; CAGTCAAGGGACTGCTTTGGCATCCAGGGCCTCCAGTCACCTCACTGCCATACATTAGAA; ATGAGACAATCAAAGCCCCCCCAGGGTGGCACACCCATCCGTTTGCTGGGGTGTGGCAGC; CACATCCAAGACTGGAGCAGCAGGCTGGCCACGCTCGGGCCAGAGAGAGCTCACAGCTGA; AGCTCTTGGAGGGAAGGGCTCTCCTCACCCTGCCAGGAAGCTTCTTAACATGTGACAGGA; CCAGGGACCAGGAGCATGGTGAAGCCAAGTGGCAGATGGGAGCCAACCTGGATGGGGGTT; TGGGGAAGGAGGGCATGTGTAGCAGAGAACTTAGGGGGGCCTCCTTGCCTTTCTCATTCT; TTTGCCCTGCATCCTGTCATTTCTGTTCTTGTCCCTCATACATCTTTGGAGAACCGGGCT; CCAGACTTTGTTCCCTGACTCATAGCTGCCGCTTGTTAGGTTAGGGTTAGATGGGGAGAG; ACAGGGCACAGAGGACCTGTCTCCCCGGCTACTCTTGCCTTATGGCTCTAGTGTGTGACC; TACAGAGCATGCTCCACAAGCCCCTGCCTCACCTCACTGTCATCACTAATAAACATCATG; CACAGTCAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000725 unedited; AAAACGTTCGNCTTTTGTAATACGACTCACTATAGGCGGCCGCATAACTTCGTATAGCAT; ACATTATACGAAGTTATGGATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGG; TGGGGGGAGCGGTGATCTGAGCTCCGAGCAGCTGGTCTTCGCGGCTCGCTCCCTCCTTCG; CGCTCTCTCGCTCCCTGCCGCCGCCCGCAGGGCTGCGGGGCTCGGTGGCATCTCCCGGGC; GCGGCCCGCAGTCCTTGCCCCTGCCTCCGGGCCGCTCCCGCCCCCGGCGCCGCTCGCTCC; CCCGACCCGGACTCCCCCATGTATGACGACTCCTACGTGCCCGGGTTTGAAGACCTCGGA; GCAGGTTCAGCCGACTCCTAACCAGCCGGCCATCTCTGGACTCAGACCGTCTCCTTGGNA; GAGACCGGGAGAGTGCCCCGCGTGGAGTANAGAGCCCAGCTCAACAACAGCTCTGAAAGG; CCAAGCCACACCTGTGGCATTTGCGGTGAAGACCCATGGCAGCTCCCGTGGCCTTCTTGA; TGAAGAGTGGCCCAGTCAGGGCTTTGAGTCAACTTTGAGCCCAAGGATTTCTGCCCCTTA; AGAGGAGTTCAACCATGACTGTGGGATCGGCCGGCTAGTGAAAAAGAGGCGGGGACCATT; GCCTTNTTCCCCAAACCCAAAAGCCCTGGGAGACTTCGGCTCTCACCCGAGCCCAAGGGC; CGGGATCTGGGAAACCTTTCCGCCTGGATGAACTTTGGAAACGAAGCTCTCCCTCGCCTT; TTTTGGCAAGCGAAACCAAAGCCGGCGGGACACGTTCCCCCCTTGAACTGGGGCCCCTCC; ATTCGGCCTGTGGTGCTCGGGGAACCCTTCCTGAAGGTTTTAAGGGCCAAACCTGGTCCA; AAGGCCTCTTTG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000725 unedited; ACCCTATACTTTGNNACCGCGGCCGCATNCTANGATCGACAGNCTTGATATCGGTACCGG; GCCCCCCCTCGAAGTTCAGGCCCTTATGGCCGGATCCAGCGCTGGAGAGTTTTTTTTTTT; TTTTTGACTGTGCATGATGTTTATTAGTGATGACAGTGAGGTGAGGCAGGGGCTTGTGGA; GCATGCTCTGTAGGTCACACACTAGAGCCATAAGGCAAGAGTAGCCGGGGAGACAGGTCC; TCTGTGCCCTGTCTCTCCCCATCTAACCCTAACCTAACAAGCGGCAGCTATGAGTCAGGG; AACAAAGTCTGGAGCCCGGTTCTCCAAAGATGTATGAGGGACAAGAACAGAAATGACAGG; ATGCAGGGCAAAAGAATGAGAAAGGCAAGGAGGCCCCCCTAAGTTCTCTGCTACACATGC; CCTCCTTCCCCAAACCCCCATCCAGGTTGGCTCCCATCTGCCACTTGGCTTCACCATGCT; CCTGGTCCCTGGTCCTGTCACATGTTAAGAAGCTTCCTGGCAGGGTGAGGAGAGCCCTTC; CCTCCAAGAGCTTCAGCTGTGAGCTCTCTCTGGCCCGAGCGTGGCCAGCCTGCTGCTCCA; GTCTTGGATGTGGCTGCCACACCCCAGCAAACGGATGGGTGTGCCACCCTGGGGGGGCTT; TGATTGTCTCATTTCTAATGTATGGCAGTGAGGTGACTGGAGGCCCTGGATGCCAAAGCA; GTCCCTTGACTGTCAGGACTTGTGCCTGCTCAGGGACCTTGTTTCTTTCTCATGCCTTGG; TGCCCACCCCCCAACCCTTTGGCACTTTTATGGGGGCATCCCCTTCCCTGATTCCGGTGG; GGAAGAACCCTGCTAAGGTGATCCTGCCCTGAAGGCCCTTCCACTTGGCGCCTCTTCCTG; TGTGGGC;
• Restriction Sites: Please inquire
• ACCN: NM_000725
• Insert Size: 1455 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found that the protein associated with this clone matches NM_000725 perfectly.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000725.2, NP_000716.2
• RefSeq Size: 2714 bp
• RefSeq ORF: 1455 bp
• Locus ID: 784
• UniProt ID: P54284
• Cytogenetics: 12q13.12
• Domains: Ca_channel_B, GuKc
• Protein Families: Druggable Genome, Ion Channels: Other
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway
• Summary: This gene encodes a regulatory beta subunit of the voltage-dependent calcium channel. Beta subunits are composed of five domains, which contribute to the regulation of surface expression and gating of calcium channels and may also play a role in the regulation of transcription factors and calcium transport. [provided by RefSeq, Oct 2011]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.