Cytochrome P450 3A5 (CYP3A5) (NM_000777) Human Untagged Clone

CAT#: SC120801

CYP3A5 (untagged)-Human cytochrome P450, family 3, subfamily A, polypeptide 5 (CYP3A5), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Cytochrome P450 3A5
• Synonyms: CP35; CYPIIIA5; P450PCN3; PCN3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120801 sequence for NM_000777 edited (data generated by NextGen Sequencing)
  ATGGACCTCATCCCAAATTTGGCGGTGGAAACCTGGCTTCTCCTGGCTGTCAGCCTGGTG
  CTCCTCTATCTATATGGGACCCGTACACATGGACTTTTTAAGAGACTGGGAATTCCAGGG
  CCCACACCTCTGCCTTTGTTGGGAAATGTTTTGTCCTATCGTCAGGGTCTCTGGAAATTT
  GACACAGAGTGCTATAAAAAGTATGGAAAAATGTGGGGAACGTATGAAGGTCAACTCCCT
  GTGCTGGCCATCACAGATCCCGACGTGATCAGAACAGTGCTAGTGAAAGAATGTTATTCT
  GTCTTCACAAATCGAAGGTCTTTAGGCCCAGTGGGATTTATGAAAAGTGCCATCTCTTTA
  GCTGAGGATGAAGAATGGAAGAGAATACGGTCATTGCTGTCTCCAACCTTCACCAGCGGA
  AAACTCAAGGAGATGTTCCCCATCATTGCCCAGTATGGAGATGTATTGGTGAGAAACTTG
  AGGCGGGAAGCAGAGAAAGGCAAGCCTGTCACCTTGAAAGACATCTTTGGGGCCTACAGC
  ATGGATGTGATTACTGGCACATCATTTGGAGTGAACATCGACTCTCTCAACAATCCACAA
  GACCCCTTTGTGGAGAGCACTAAGAAGTTCCTAAAATTTGGTTTCTTAGATCCATTATTT
  CTCTCAATAATACTCTTTCCATTCCTTACCCCAGTTTTTGAAGCATTAAATGTCTCTCTG
  TTTCCAAAAGATACCATAAATTTTTTAAGTAAATCTGTAAACAGAATGAAGAAAAGTCGC
  CTCAACGACAAACAAAAGCACCGACTAGATTTCCTTCAGCTGATGATTGACTCCCAGAAT
  TCGAAAGAAACTGAGTCCCACAAAGCTCTGTCTGATCTGGAGCTCGCAGCCCAGTCAATA
  ATCTTCATTTTTGCTGGCTATGAAACCACCAGCAGTGTTCTTTCCTTCACTTTATATGAA
  CTGGCCACTCACCCTGATGTCCAGCAGAAACTGCAAAAGGAGATTGATGCAGTTTTGCCC
  AATAAGGCACCACCTACCTATGATGCCGTGGTACAGATGGAGTACCTTGACATGGTGGTG
  AATGAAACACTCAGATTATTCCCAGTTGCTATTAGACTTGAGAGGACTTGCAAGAAAGAT
  GTTGAAATCAATGGGGTATTCATTCCCAAAGGGTCAATGGTGGTGATTCCAACTTATGCT
  CTTCACCATGACCCAAAGTACTGGACAGAGCCTGAGGAGTTCCGCCCTGAAAGGTTCAGT
  AAGAAGAAGGACAGCATAGATCCTTACATATACACACCCTTTGGAACTGGACCCAGAAAC
  TGCATTGGCATGAGGTTTGCTCTCATGAACATGAAACTTGCTCTAATCAGAGTCCTTCAG
  AACTTCTCCTTCAAACCTTGTAAAGAAACACAGATCCCCTTGAAATTAGACACGCAAGGA
  CTTCTTCAACCAGAAAAACCCATTGTTCTAAAGGTGGATTCAAGAGATGGAACCCTAAGT
  GGAGAATGA
  
  Clone variation with respect to NM_000777.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000777 unedited
  NGGGTCAGGATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGGGAAGC
  TCCAGGCAAACAGCCCAGCAAACAGCAGCACTCAGCTAAAAGGAAGACTCACAGAACACA
  GTTGAAGAAGGAAAGTGGCGATGGACCTCATCCCAAATTTGGCGGTGGAAACCTGGCTTC
  TCCTGGCTGTCAGCCTGGTGCTCCTCTATCTATATGGGACCCGTACACATGGACTTTTTA
  AGAGACTGGGAATTCCAGGGCCCACACCTCTGCCTTTGTTGGGAAATGTTTTGTCCTATC
  GTCAGGGTCTCTGGAAATTTGACACAGAGTGCTATAAAAAGTATGGAAAAATGTGGGGAA
  CGTATGAAGGTCAACTCCCTGTGCTGGCCATCACAGATCCCGACGTGATCAGAACAGTGC
  TAGTGAAAGAATGTTATTCTGTCTTCACAAATCGAAGGTCTTTAGGCCCAGTGGGATTTA
  TGAAAAGTGCCATCTCTTTAGCTGAGGATGAAGAATGGAAGAGAATACGGTCATTGCTGT
  CTCCAACCTTCACCAGCGGAAAACTCAAGGAGATGTTCCCCATCATTGCCCAGTATGGAG
  ATGTATTGGTGAGAAACTTGAGGCGGGAAGCAGAGAAAGGCAAGCCTGTCACCTTGAAAG
  ACATCTTTGGGGCCTACAGCATGGATGTGATTACTGGCACATCATTTGGAGTGAACATCG
  ACTCTCTCAACAATCCACAAGACCCCTTTGTGGAGAGCACTAAGAAGTTCCTAANATTTG
  GTTTCTTAGATCCATTATTTCTCTCAATATACTCTNTCCATTCCTTACCCCAGTNTTTGA
  GCATTAATGTCTCTCTGTTTCCAAAGATACATANATTTTTTAAGTAATCTGTAACAGAAT
  GAAGAAAGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000777 unedited
  CCTTGTTAGGCCCTCTTTTTTCAGGTTTTATTGACTAAGTTGAAATCTCTGGTGTTCTGG
  GGCACAGCTTTCTTGAAGACCAAAGTAGAAGTCCTTAGAATAACTCATTCTCCACTTAGG
  GTTCCATCTCTTGAATCCACCTTTAGAACAATGGGTTTTTCTGGTTGAAGAAGTCCTTGC
  GTGTCTAATTTCAAGGGGATCTGTGTTTCTTTACAAGGTTTGAAGGAGAAGTTCTGAAGG
  ACTCTGATTAGAGCAAGTTTCATGTTCATGAGAGCAAACCTCATGCCAATGCAGTTTCTG
  GGTCCAGTTCCAAAGGGTGTGTATATGTAAGGATCTATGCTGTCCTTCTTCTTACTGAAC
  CTTTCAGGGCGGAACTCCTCAGGCTCTGTCCAGTACTTTGGGTCATGGTGAAGAGCATAA
  GTTGGAATCACCACCATTGACCCTTTGGGAATGAATACCCCATTGATTTCAACATCTTTC
  TTGCAAGTCCTCTCAAGTCTAATAGCAACTGGGAATAATCTGAGTGTTTCATTCACCACC
  ATGTCAAGGTACTCCATCTGTACCACGGCATCATAGGTAGGTGGTGCCTTATTGGGCAAA
  ACTGCATCAATCTCCTTTTGCAGTTTCTGCTGGACATCAGGGTGAGTGGCCAGTTCATAT
  AAAGTGAAGGAAAGAACACTGCTGGTGGTTTCATAGCCAGCAAAAATGAAGATTATTGAC
  TGNGCTGCGAGCTCCAGATCAGACAGAGCTTTGTGGGACTCAGTTTCTTTCGAATTCTGG
  GAGTCAATCATCAGCTGAAGGAAATCTAGTCGGTGCTTTTGTTTGTCGTTGAGGCGACTT
  TTCTTCATTCTGTTTACANGATTACTTAAAAATTTATGGTATCTTTTGGAACAGAGAGAC
  ATTAATGCTTCANAACTGNNGTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000777
• Insert Size: 1800 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000777.2, NP_000768.1
• RefSeq Size: 1707 bp
• RefSeq ORF: 1509 bp
• Locus ID: 1577
• UniProt ID: P20815
• Cytogenetics: 7q22.1
• Domains: p450
• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Drug metabolism - cytochrome P450, Drug metabolism - other enzymes, Linoleic acid metabolism, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Retinol metabolism
• Gene Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The encoded protein metabolizes drugs as well as the steroid hormones testosterone and progesterone. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Two pseudogenes of this gene have been identified within this cluster on chromosome 7. Expression of this gene is widely variable among populations, and a single nucleotide polymorphism that affects transcript splicing has been associated with susceptibility to hypertensions. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]
  Transcript Variant: This variant (1) encodes the longest isoform (1).