DNER (NM_139072) Human Untagged Clone

CAT#: SC120614

DNER (untagged)-Human delta/notch-like EGF repeat containing (DNER)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DNER
• Synonyms: bet; UNQ26
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120614 sequence for NM_139072 edited (data generated by NextGen Sequencing)
  ATGCAGCCCCGCCGCGCCCAGGCGCCCGGTGCGCAGCTGCTGCCCGCGCTGGCCCTGCTG
  CTGCTGCTGCTCGGAGCGGGGCCCCGAGGCAGCTCCCTGGCCAACCCGGTGCCCGCCGCG
  CCCTTGTCTGCGCCCGGGCCGTGCGCCGCGCAGCCCTGCCGGAATGGGGGTGTGTGCACC
  TCGCGCCCTGAGCCGGACCCGCAGCACCCGGCCCCCGCCGGCGAGCCTGGGTACAGCTGC
  ACCTGCCCCGCCGGGATCTCCGGCGCCAACTGCCAGCTTGTTGCAGATCCTTGTGCCAGC
  AACCCTTGTCACCATGGCAACTGCAGCAGCAGCAGCAGCAGCAGCAGCGATGGCTACCTC
  TGCATTTGCAATGAAGGCTATGAAGGTCCCAACTGTGAACAGGCACTTCCCAGTCTCCCA
  GCCACTGGCTGGACCGAATCCATGGCACCCCGACAGCTTCAGCCTGTTCCTGCTACTCAG
  GAGCCTGACAAAATCCTGCCTCGCTCTCAGGCAACGGTGACACTGCCTACCTGGCAGCCG
  AAAACAGGGCAGAAAGTTGTAGAAATGAAATGGGATCAAGTGGAGGTGATCCCAGATATT
  GCCTGTGGGAATGCCAGTTCTAACAGCTCTGCGGGTGGCCGCCTGGTATCCTTTGAAGTG
  CCACAGAACACCTCAGTCAAGATTCGGCAAGATGCCACTGCCTCACTGATTTTGCTCTGG
  AAGGTCACGGCCACAGGATTCCAACAGTGCTCCCTCATAGATGGACGAAGTGTGACCCCC
  CTTCAGGCTTCAGGGGGACTGGTCCTCCTGGAGGAGATGCTCGCCTTGGGGAATAATCAC
  TTTATTGGTTTTGTGAATGATTCTGTGACTAAGTCTATTGTGGCTTTGCGCTTAACTCTG
  GTGGTGAAGGTCAGCACCTGTGTGCCGGGGGAGAGTCACGCAAATGACTTGGAGTGTTCA
  GGAAAAGGAAAATGCACCACGAAGCCGTCAGAGGCAACTTTTTCCTGTACCTGTGAGGAG
  CAGTACGTGGGTACTTTCTGTGAAGAATACGATGCTTGCCAGAGGAAACCTTGCCAAAAC
  AACGCGAGCTGTATTGATGCAAATGAAAAGCAAGATGGGAGCAATTTCACCTGTGTTTGC
  CTTCCTGGTTATACTGGAGAGCTTTGCCAGTCCAAGATTGATTACTGCATCCTAGACCCA
  TGCAGAAATGGAGCAACATGCATTTCCAGTCTCAGTGGATTCACCTGCCAGTGTCCAGAA
  GGATACTTCGGATCTGCTTGTGAAGAAAAGGTGGACCCCTGCGCCTCGTCTCCGTGCCAG
  AACAACGGCACCTGCTATGTGGACGGGGTACACTTTACCTGCAACTGCAGCCCGGGCTTC
  ACAGGGCCGACCTGTGCCCAGCTTATTGACTTCTGTGCCCTCAGCCCCTGTGCTCATGGC
  ACGTGCCGCAGCGTGGGCACCAGCTACAAATGCCTCTGTGATCCAGGTTACCATGGCCTC
  TACTGTGAGGAGGAATATAATGAGTGCCTCTCCGCTCCATGCCTGAATGCAGCCACCTGC
  AGGGACCTCGTTAATGGCTATGAGTGTGTGTGCCTGGCAGAATACAAAGGAACACACTGT
  GAATTGTACAAGGATCCCTGCGCTAACGTCAGCTGTCTGAACGGAGCCACCTGTGACAGC
  GACGGCCTGAATGGCACGTGCATCTGTGCACCCGGGTTTACAGGTGAAGAGTGCGACATT
  GACATAAATGAATGTGACAGTAACCCCTGCCACCATGGTGGGAGCTGCCTGGACCAGCCC
  AATGGTTATAACTGCCACTGCCCGCATGGTTGGGTGGGAGCAAACTGTGAGATCCACCTC
  CAATGGAAGTCCGGGCACATGGCGGAGAGCCTCACCAACATGCCACGGCACTCCCTCTAC
  ATCATCATTGGAGCCCTCTGCGTGGCCTTCATCCTTATGCTGATCATCCTGATCGTGGGG
  ATTTGCCGCATCAGCCGCATTGAATACCAGGGTTCTTCCAGGCCAGCCTATGAGGAGTTC
  TACAACTGCCGCAGCATCGACAGCGAGTTCAGCAATGCCATTGCATCCATCCGGCATGCC
  AGGTTTGGAAAGAAATCCCGGCCTGCAATGTATGATGTGAGCCCCATCGCCTATGAAGAT
  TACAGTCCTGATGACAAACCCTTGGTCACACTGATTAAAACTAAAGATTTGTAA
  
  Clone variation with respect to NM_139072.3
  124 c=>t;231 c=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_139072 unedited
  GAGGCGCCACCGTCGCTCTCGCAGCTCTCGTCGCCACTGCCACCGCCGCCGCCGTCACTG
  CGTCCTGGCTCCGGCTCCCGCGCCCTCCCGGCCGGCCATGCAGCCCCGCCGCGCCCAGGC
  GCCCGGTGCGCAGCTGCTGCCCGCGCTGGCCCTGCTGCTGCTGCTGCTCGGAGCGGGGCC
  CCGAGGCAGCTCCCTGGCCAACCCGGTGCCCGCCGCGCCCTTGTCTGCGCCCGGGCCGTG
  CGCCGCGCAGCCCTGCCGGAATGGGGGTGTGTGCACCTCGCGCCCTGAGCCGGACCCGCA
  GCACCCGGCCCCCGCCGGCGAGCCTGGGTACAGCTGCACCTGCCCCGCCGGGATCTCCGG
  CGCCAACTGCCAGCTTGTTGCAGATCCTTGTGCCAGCAACCCTTGTCACCATGGCAACTG
  CAGCAGCAGCAGCAGCAGCAGCAGCGATGGCTACCTCTGCATTTGCAATGAAGGCTATGA
  AGGTCCCAACTGTGAACAGGCACTTCCCAGTCTCCCAGCCACTGGCTGGACCGAATCCAT
  GGCACCCCGACAGCTTCAGCCTGTTCCTGCTACTCAGGAGCCTGACAAAATCCTGCCTCG
  CTCTCANGCAACGGTGACACTGCCTACCTGGCAGCCGAAAACAGGGCAGAAAGTTGTAGA
  AATGAAATGGGATCAAGTGGAGGTGATCCCAGATATTGGCCTGTGGGAATGCCAGTTCTA
  ACAGCTCTGCGGGTGGNCGCCTGGTATTCTTTGAAGTGCCACAGAACACCTCAGTCAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_139072 unedited
  GACTATGNNACCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTTCCTT
  GACTTGATCACATTTATTCAATATGGTTGGGAAATTAGCAAGAATCAGAAGAAGCACATA
  TCAATCAAATACAGCCACAAAAACATCCTGGAGATAAATAAAGCTGCACTATGAGAAATA
  CTCACTACTGAAGGAACCAGTACTCTCTTACATTTACTACCTCTTAGCAACAAACAACTC
  AAATGTAAATTTAATTTACTCACAATATAGAACCCTTGACTTTTTAAAGAAAAATATAGA
  TTCAAATCAATCAGAATTTGCCTCGCTAGGCCTTTTCGTTACGTATGTTTTTGAGGCCTA
  GTTCGACGACTGTTACGAAAATGACAAAAACAAACTCGAAAGAATGTTTCCACAAAGTTC
  AACAAAACAGTGCAGAAATAAGTTACTTACCCATGCACAGAATAAGGCAAAGGCTAATTT
  CATTCGGGTTAGGAATAGTAAATTTAACTAGTTTCTTTTCCACAAAACGAACATTTCAGA
  CTTCTTTTTAAGTAACGGGTATACTCTATTATACATCATATGCTACGTTACGTGGTTTTC
  CTAACTCTACTCAGAAAGCCGGCACTCAAACGTCAAACAGAAACTTCTCCTTGATTAGAC
  TTATTCTGTAACAACCACAGAAATTAATCTGGGTCTCGTGATAGTGTCTACAGTGANAAG
  AGCACACACTAGTAGAAGCACACAGTTTAGAGAGCTGAAGGTACATTAAAACATCGTCTA
  TAGGTTTCACAAATTTTGGTTTTAAATATTTTTTCCAAACTAAAAGCTGCAGAAATATAG
  TCTTAANATTCTACTGAAAACTCTTGAGCAGCTGCATTTTAATTTCTTAAGCTTTTTATT
  TCTTAAAAAATTAAATGAGGGTGGATCTCATCTTTTGAAAATAATCAAAAAAGATACAAT
  CTTTAGTTTAATCAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_139072
• Insert Size: 3500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139072.2, NP_620711.2
• RefSeq Size: 3308 bp
• RefSeq ORF: 2214 bp
• Locus ID: 92737
• UniProt ID: Q8NFT8
• Cytogenetics: 2q36.3
• Domains: EGF_CA, EGF, EGF
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: Activator of the NOTCH1 pathway. May mediate neuron-glia interaction during astrocytogenesis (By similarity).[UniProtKB/Swiss-Prot Function]