Galactosylceramidase (GALC) (NM_000153) Human Untagged Clone

CAT#: SC120078

GALC (untagged)-Human galactosylceramidase (GALC), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Galactosylceramidase
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000153, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTGAGTGGCTACTCTCGGCTTCCTGGCAACGCCGAGCGAAAGCTATGACTGCGGCCGCGGGTTCGG
  CGGGCCGCGCCGCGGTGCCCTTGCTGCTGTGTGCGCTGCTGGCGCCCGGCGGCGCGTACGTGCTCGACGA
  CTCCGACGGGCTGGGCCGGGAGTTCGACGGCATCGGCGCGGTCAGCGGCGGCGGGGCAACCTCCCGACTT
  CTAGTAAATTACCCAGAGCCCTATCGTTCTCAGATATTGGATTATCTCTTTAAGCCGAATTTTGGTGCCT
  CTTTGCATATTTTAAAAGTGGAAATAGGTGGTGATGGGCAGACAACAGACGGCACTGAGCCCTCCCACAT
  GCATTATGCACTAGATGAGAATTATTTCCGAGGATACGAGTGGTGGTTGATGAAAGAAGCTAAGAAGAGG
  AATCCCAATATTACACTCATTGGGTTGCCATGGTCATTCCCTGGATGGCTGGGAAAAGGTTTCGACTGGC
  CTTATGTCAATCTTCAGCTGACTGCCTATTATGTCGTGACCTGGATTGTGGGCGCCAAGCGTTACCATGA
  TTTGGACATTGATTATATTGGAATTTGGAATGAGAGGTCATATAATGCCAATTATATTAAGATATTAAGA
  AAAATGCTGAATTATCAAGGTCTCCAGCGAGTGAAAATCATAGCAAGTGATAATCTCTGGGAGTCCATCT
  CTGCATCCATGCTCCTTGATGCCGAACTCTTCAAGGTGGTTGATGTTATAGGGGCTCATTATCCTGGAAC
  CCATTCAGCAAAAGATGCAAAGTTGACTGGGAAGAAGCTTTGGTCTTCTGAAGACTTTAGCACTTTAAAT
  AGTGACATGGGTGCAGGCTGCTGGGGTCGCATTTTAAATCAGAATTATATCAATGGCTATATGACTTCCA
  CAATCGCATGGAATTTAGTGGCTAGTTACTATGAACAGTTGCCTTATGGGAGATGCGGGTTGATGACGGC
  CCAGGAGCCATGGAGTGGGCACTACGTGGTAGAATCTCCTGTCTGGGTATCAGCTCATACCACTCAGTTT
  ACTCAACCTGGCTGGTATTACCTGAAGACAGTTGGCCATTTAGAGAAAGGAGGAAGCTACGTAGCTCTGA
  CTGATGGCTTAGGGAACCTCACCATCATCATTGAAACCATGAGTCATAAACATTCTAAGTGCATACGGCC
  ATTTCTTCCTTATTTCAATGTGTCACAACAATTTGCCACCTTTGTTCTTAAGGGATCTTTTAGTGAAATA
  CCAGAGCTACAGGTATGGTATACCAAACTTGGAAAAACATCCGAAAGATTTCTTTTTAAGCAGCTGGATT
  CTCTATGGCTCCTTGACAGCGATGGCAGTTTCACACTGAGCCTGCATGAAGATGAGCTGTTCACACTCAC
  CACTCTCACCACTGGTCGCAAAGGCAGCTACCCGCTTCCTCCAAAATCCCAGCCCTTCCCAAGTACCTAT
  AAGGATGATTTCAATGTTGATTACCCATTTTTTAGTGAAGCTCCAAACTTTGCTGATCAAACTGGTGTAT
  TTGAATATTTTACAAATATTGAAGACCCTGGCGAGCATCACTTCACGCTACGCCAAGTTCTCAACCAGAG
  ACCCATTACGTGGGCTGCCGATGCATCCAACACAATCAGTATTATAGGAGACTACAACTGGACCAATCTG
  ACTATAAAGTGTGATGTATACATAGAGACCCCTGACACAGGAGGTGTGTTCATTGCAGGAAGAGTAAATA
  AAGGTGGTATTTTGATTAGAAGTGCCAGAGGAATTTTCTTCTGGATTTTTGCAAATGGATCTTACAGGGT
  TACAGGTGATTTAGCTGGATGGATTATATATGCTTTAGGACGTGTTGAAGTTACAGCAAAAAAATGGTAT
  ACACTCACGTTAACTATTAAGGGTCATTTCACCTCTGGCATGCTGAATGACAAGTCTCTGTGGACAGACA
  TCCCTGTGAATTTTCCAAAGAATGGCTGGGCTGCAATTGGAACTCACTCCTTTGAATTTGCACAGTTTGA
  CAACTTTCTTGTGGAAGCCACACGCTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000153 unedited
  AAAAGATTCGGATTTGTACACGATTCACTATAGGCGGCCGCGAATTCGCACGAGGCCTCG
  TGCCGAATTCGGCACGAGGGCGGCGTCAGCATCAGCGGCCTCCTGCCCGTATCTATCGTG
  GCGGCGACGGGACCCGCCTCCCTGGGCGCCGGAGTCATGTGACCCACACAATGGCTGAGT
  GGCTACTCTCGGCTTCCTGGCAACGCCGAGCGAAAGCTATGACTGCGGCCGCGGGTTCGG
  CGGGCCGCGCCGCGGTGCCCTTGCTGCTGTGTGCGCTGCTGGCGCCCGGCGGCGCGTACG
  TGCTCGACGACTCCGACGGGCTGGGCCGGGAGTTCGACGGCATCGGCGCGGTCAGCGGCG
  GCGGGGCAACCTCCCGACTTCTAGTAAATTACCCAGAGCCCTATCGTTCTCAGATATTGG
  ATTATCTCTTTAAGCCGAATTTTGGTGCCTCTTTGCATATTTTAAAAGTGGAAATAGGTG
  GTGATGGGCAGACAACAGACGGCACTGAGCCCTCCCACATGCATTATGCACTAGATGAGA
  ATTATTTCCGAGGATACGAGTGGTGGTTGATGAAAGAAGCTAAGAAGAGGAATCCCAATA
  TTACACTCATTGGGTTGCCATGGTCATTCCCTGGATGGCTGGGAAAAGGTTTCGACTGGC
  CTTATGTCAATCTTCAGCTGACTGCCTATTATGTCGTGACCTGGATTGTGGGCGCCAAGC
  GTTACCATGATTTGGACATTGATTATATTGGAATTTGGAATGAGAGGTCATATAATGCCA
  ATTATATTAAGATATTAAGAAAAATGGCTGATTATCAAGGTCTCCAGCGAGTGAAATCAT
  AGCAGTGGATATCTCTGGGAGTCCATCTCTGCATCCATGCTNCTTGATGCCCGACTCTTC
  AGGGTGTTN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000153 unedited
  AGCTATGNNACCGCGGCCGCAATCTAANGATCGGTTTTTTTTTTTTTTTTTTTGTAAATG
  TAGACAGTTTTAATTGTAGTATCAGAAACTGGTGGGGAGGAAACAAATTGTGGTATATTC
  ATACAATGGAAAACTCTTCAGAAATAAGAAGGAACAAACCACTGAATCACACAACATGGA
  CCAATCTCAAATCATTATGCTGATGGAAAGAAACCATTCATAAGAATACACAGTACATGA
  CGCCGCTTTCATGATGTTCTGGAACAAAGAAAACTAACCTATAGTGATAGAATTCCTATC
  AATGGCTGCCAACAATTGGGAGTGAAAGGAACTGACTGAGCAGGTATACAAGAGAACCTT
  CTGGGGTGATGGAAATATTCTGAAGCTTGACTGGAGTGTTGGTTACATGGGTATATCAAT
  TTATCAAAACTCACTGAATTGTATATTTAAAGTAGGAACATTTTATTGTAAATAAATTAC
  ACCTCTATAATGTTTGTTTTAAAAAATATTAGATACATCAGCTGGAAATCACTTGGTTTT
  AACAAAAAATTTTCTCTGTAATCTTATTTATTCTAACACTTTTCTTGTCTTTAAAAGCAA
  TGCAAAACTTCACTGTAGCTAAGCCCATGTTAAACTCTTACAAATTCCTACATAGTAGAA
  CTAGTTCAATGAGTAATTCTAAAAAAAATTGTAGATTTACTTTAACCTCATGGATAACTG
  ATTTGCTACCTAGAGCCCCATTAATGCGTTAGATAAACCCTACCCCAGGTGAACATGCTC
  ACGCTTATTAAAAAATAAACACTTTTTGACTCTAACAAGAACCCCACCCTAAGGAGAGAA
  CCCATCCCGTGGAGTTACAAAAAGGCGCAATGGCTCCCTCTCTTCCCCACCCAAGGGACA
  AAAAAGCTCTCTTATCTGGGTGAACTCGTTACCACCGCCCACCGGGAGGCACCACCCCAA
  GCCCCGGCGCAGAGGCCTGGGACAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000153
• Insert Size: 3870 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000153.1, NP_000144.1
• RefSeq Size: 3897 bp
• RefSeq ORF: 3897 bp
• Locus ID: 2581
• UniProt ID: P54803
• Cytogenetics: 14q31.3
• Domains: Glyco_hydro_59
• Protein Families: Druggable Genome
• Protein Pathways: Lysosome, Metabolic pathways, Sphingolipid metabolism
• Gene Summary: This gene encodes a lysosomal protein which hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. Mutations in this gene have been associated with Krabbe disease, also known as globoid cell leukodystrophy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.