TBX5 (NM_000192) Human Untagged Clone

SKU SC120046

TBX5 (untagged)-Human T-box 5 (TBX5), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: TBX5
• Synonyms: HOS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120046 sequence for NM_000192 edited (data generated by NextGen Sequencing); ATGGCCGACGCAGACGAGGGCTTTGGCCTGGCGCACACGCCTCTGGAGCCTGACGCAAAA; GACCTGCCCTGCGATTCGAAACCCGAGAGCGCGCTCGGGGCCCCCAGCAAGTCCCCGTCG; TCCCCGCAGGCCGCCTTCACCCAGCAGGGCATGGAGGGAATCAAAGTGTTTCTCCATGAA; AGAGAACTGTGGCTAAAATTCCACGAAGTGGGCACGGAAATGATCATAACCAAGGCTGGA; AGGCGGATGTTTCCCAGTTACAAAGTGAAGGTGACGGGCCTTAATCCCAAAACGAAGTAC; ATTCTTCTCATGGACATTGTACCTGCCGACGATCACAGATACAAATTCGCAGATAATAAA; TGGTCTGTGACGGGCAAAGCTGAGCCCGCCATGCCTGGCCGCCTGTACGTGCACCCAGAC; TCCCCCGCCACCGGGGCGCATTGGATGAGGCAGCTCGTCTCCTTCCAGAAACTCAAGCTC; ACCAACAACCACCTGGACCCATTTGGGCATATTATTCTAAATTCCATGCACAAATACCAG; CCTAGATTACACATCGTGAAAGCGGATGAAAATAATGGATTTGGCTCAAAAAATACAGCG; TTCTGCACTCACGTCTTTCCTGAGACTGCGTTTATAGCAGTGACTTCTTACCAGAACCAC; AAGATCACGCAATTAAAGATTGAGAATAATCCCTTTGCCAAAGGATTTCGGGGCAGTGAT; GACATGGAGCTGCACAGAATGTCAAGAATGCAAAGTAAAGAATATCCCGTGGTCCCCAGG; AGCACCGTGAGGCAAAAAGTGGCCTCCAACCACAGTCCTTTCAGCAGCGAGTCTCGAGCT; CTCTCCACCTCATCCAATTTGGGGTCCCAATACCAGTGTGAGAATGGTGTTTCCGGCCCC; TCCCAGGACCTCCTGCCTCCACCCAACCCATACCCACTGCCCCAGGAGCATAGCCAAATT; TACCATTGTACCAAGAGGAAAGAGGAAGAATGTTCCACCACAGACCATCCCTATAAGAAG; CCCTACATGGAGACATCACCCAGTGAAGAAGATTCCTTCTACCGCTCTAGCTATCCACAG; CAGCAGGGCCTGGGTGCCTCCTACAGGACAGAGTCGGCACAGCGGCAAGCTTGCATGTAT; GCCAGCTCTGCGCCCCCCAGCGAGCCTGTGCCCAGCCTAGAGGACATCAGCTGCAACACG; TGGCCAAGCATGCCTTCCTACAGCAGCTGCACCGTCACCACCGTGCAGCCCATGGACAGG; CTACCCTACCAGCACTTCTCCGCTCACTTCACCTCGGGGCCCCTGGTCCCTCGGCTGGCT; GGCATGGCCAACCATGGCTCCCCACAGCTGGGAGAGGGAATGTTCCAGCACCAGACCTCC; GTGGCCCACCAGCCTGTGGTCAGGCAGTGTGGGCCTCAGACTGGCCTGCAGTCCCCTGGC; ACCCTTCAGCCCCCTGAGTTCCTCTACTCTCATGGCGTGCCAAGGACTCTATCCCCTCAT; CAGTACCACTCTGTGCACGGAGTTGGCATGGTGCCAGAGTGGAGCGACAATAGCTAA; ; Clone variation with respect to NM_000192.3; 648 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000192 unedited; CCGACACTCGTCATTTTGTNAATACGNACTTTCTNTNAGGGNNCGGCCGCGNAATTTCGG; CACCAGGATAAACAGTAATATTTAATTTGTCGGAGACCACAAACCAACCTTGAGCTGGGA; GGTACGTGCTCTTCTTGACAGACGTTGGAAGAAGACCTGGCCTAAAGAGGTCTCTTTTGG; TGGTCCTTTTCAAAGTCTTCACCTGAGCCCTGCTCTCCAGCGAGGCGCACTCCTGGCTTT; TGCGCTCCAAAGAAGAGGTGGGATAGTTGGAGAGCAGAACCTTGCGCGGGCACAGGGCCC; TGGGCGCACCATGGCCGACGCAGACGAGGGCTTTGGCCTGGCGCACACGCCTCTGGAGCC; TGACGCAAAAGACCTGCCCTGCGATTCGAAACCCGAGAGCGCGCTCGGGGCCCCCAGCAA; GTCCCCGTCGTCCCCGCAGGCCGCCTTCACCCAGCAGGGCATGGAGGGAATCAAAGTGTT; TCTCCATGAAAGAGAACTGTGGCTAAAATTCCACGAAGTGGGCACGGAAATGATCATAAC; CAAGGCTGGAAGGCGGATGTTTCCCAGTTACAAAGTGAAGGTGACGGGCCTTAATCCCAA; AACGAAGTACATTCTTCTCATGGACATTGTACCTGCCGACGATCACAGATACAAATTCGC; AGATAATAAATGGTCTGTGACGGGCAAAGCTGAGCCCGCCATGCCTGGCCGCCTGTACGT; GCACCCAGACTCCCCCGCCACCGGGGCGCATTGGATGAAGCAGCTCGTCTCCTTCCCAGA; AACTCAGCTCACCACAACCACCTGGACCCTTTGGGCATATTATTCTAAATTCATGCANCA; ATACCAGCCTAGATTACACATCGTGAAAGCGGATGAAAATATGGATTNGNCTCAAAAATA; CACCGTCT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000192 unedited; GGAAAAAAACAATATATTAACAAAAAAACACAAACTTGGTTTCTGAGTATCAATAAAATT; AAAAGCTCTTGGCCAGCTCCTATGCTGGGTTTCATTTCATGTTATTACTTAATTAGAATT; CCTATTTATAAATAAATAGTACCAGTAAAATATTACATCTGAAGAACCCTACCATAACAC; TTTACTTACACACTTTTTCCAATACTGTTTTCGGCTTTCAGTAAACACAGTTCTGTGTCG; GCATTGGTGTGGGCGTGGTTTCTTTGGCGAGATATCATTGCCGACTGTGCCCTGTCTCTT; CTGTAGGAACCGTGTATTTTGCTTGACATCCACTTTGCCCCGTTTTTCACGGACGTTTGG; AACGCTGGTTCCTGCCCTGTTTCCTCCTAACACATGCCTCTTTTATTAATAACCCCATTT; CGGTTTTTCCGTCTCAACTCTTCCCACACCCTTCTCGCCCCTGCCATTACATTCCTGCCG; CACCGTCCACCGCGTTTCTTTCCTCTCGCCCATTTCCGCCTTTCCCCCCTTTTTTTTCTT; TTCTCCCCCCCCCCCGACAGGCATTTTTTCAGTCTTTCCCGCGCTTTCCGGCGTCGCCCT; TTTTTTCCCCCCTTCTCCTTTTTCCCGTATCGGGCCCCCTCCTTTTCCCGCCGTTTTCCC; CCCGCCATTTTTTGGTTTTTTTTTTTCGCGCCCCCGCGTTGTGCAAAACCCGCTCTCTCC; CCCCCCTTTTTTCTCGTTGTTTTTTTCTCCCCCCTCTTTTTTCTCCTCTTGTTTTTTGTG; TCCACGTTTTCCGAATTGCCCTCGTCACCCGCCGGCTCTGCCGGCCCCTTTTTTTTTTTT; TCGCCCCCTTTTTTCCCCCTCCCCCCCGTCTTTTGT;
• Restriction Sites: NotI-NotI
• ACCN: NM_000192
• Insert Size: 1557 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000192.3, NP_000183.2
• RefSeq Size: 3921 bp
• RefSeq ORF: 1557 bp
• Locus ID: 6910
• UniProt ID: Q99593
• Cytogenetics: 12q24.21
• Domains: T-box
• Protein Families: Druggable Genome, Transcription Factors
• Summary: This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in this gene have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Variants 1 and 4 both encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.