PTEN (NM_000314) Human Untagged Clone

CAT#: SC119965

PTEN (untagged)-Human phosphatase and tensin homolog (PTEN)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PTEN
• Synonyms: 10q23del; BZS; CWS1; DEC; GLM2; MHAM; MMAC1; PTEN1; PTENbeta; TEP1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000314 edited
  GAATTCGGCACGAGGGCCAAGCGGCGGCAGAGCGAGGGGCATCAGCTACCGCCAAGTCCA
  GAGCCATTTCCATCCTGCAGAAGAAGCCCCGCCACCAGCAGCTTCTGCCATCTCTCTCCT
  CCTTTTTCTTCAGCCACAGGCTCCCAGACATGACAGCCATCATCAAAGAGATCGTTAGCA
  GAAACAAAAGGAGATATCAAGAGGATGGATTCGACTTAGACTTGACCTATATTTATCCAA
  ACATTATTGCTATGGGATTTCCTGCAGAAAGACTTGAAGGCGTATACAGGAACAATATTG
  ATGATGTAGTAAGGTTTTTGGATTCAAAGCATAAAAACCATTACAAGATATACAATCTTT
  GTGCTGAAAGACATTATGACACCGCCAAATTTAATTGCAGAGTTGCACAATATCCTTTTG
  AAGACCATAACCCACCACAGCTAGAACTTATCAAACCCTTTTGTGAAGATCTTGACCAAT
  GGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTAAAGCTGGAAAGGGACGAA
  CTGGTGTAATGATATGTGCATATTTATTACATCGGGGCAAATTTTTAAAGGCACAAGAGG
  CCCTAGATTTCTATGGGGAAGTAAGGACCAGAGACAAAAAGGGAGTAACTATTCCCAGTC
  AGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATCTGGATTATAGACCAG
  TGGCACTGTTGTTTCACAAGATGATGTTTGAAACTATTCCAATGTTCAGTGGCGGAACTT
  GCAATCCTCAGTTTGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGAC
  CCACACGACGGGAAGACAAGTTCATGTACTTTGAGTTCCCTCAGCCGTTACCTGTGTGTG
  GTGATATCAAAGTAGAGTTCTTCCACAAACAGAACAAGATGCTAAAAAAGGACAAAATGT
  TTCACTTTTGGGTAAATACATTCTTCATACCAGGACCAGAGGAAACCTCAGAAAAAGTAG
  AAAATGGAAGTCTATGTGATCAAGAAATCGATAGCATTTGCAGTATAGAGCGTGCAGATA
  ATGACAAGGAATATCTAGTACTTACTTTAACAAAAAATGATCTTGACAAAGCAAATAAAG
  ACAAAGCCAACCGATACTTTTCTCCAAATTTTAAGGTGAAGCTGTACTTCACAAAAACAG
  TAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACCAGATGTTAGTG
  ACAATGAACCTGATCATTATAGATATTCTGACACCACTGACTCTGATCCAGAGAATGAAC
  CTTTTGATGAAGATCAGCATACACAAATTACAAAAGTCTGAATTTTTTTTTATCAAGAGG
  GATAAAACACCATGAAAATAAACTTGAATAAACTGAAAATGGACCTTTTTTTTTTTAATG
  GCAATAGGACATTGTGTCAGATTACCAGTTATAGGAACAATTCTCTTTTCCTGACCAATC
  TTGTTTTACCCTATACATCCACAGGGTTTTGACACTTGTTGTCCAGTTGAAAAAAGGTTG
  TGTAGCTGTGTCATGTATATACCTTTTTGTGTCAAAAGGACATTTAAAATTCAATTAGGA
  TTAATAAAGATGGCACTTTCCCGTTTTATTCCAGTTTTATAAAAAGTGGAGACAGACTGA
  TGTGTATACGTAGGAATTTTTTCCTTTTGTGTTCTGTCACCAACTGAAGTGGCTAAAGAG
  CTTTGTGATATACTGGTTCACATCCTACCCCTTTGCACTTGTGGCAACAGATAAGTTTGC
  AGTTGGCTAAGAGAGGTTTCCGAAGGGTTTTGCTACATTCTAATGCATGTATTCGGGTTA
  GGGGAATGGAGGGAATGCTCAGAAAGGAAATAATTTTATGCTGGACTCTGGACCATATAC
  CATCTCCAGCTATTTACACACACCTTTCTTTAGCATGCTACAGTTATTAATCTGGACATT
  CGAGGAATTGGCCGCTGTCACTGCTTGTTGTTTGCGCATTTTTTTTTAAAGCATATTGGT
  GCTAGAAAAGGCAGCTAAAGGAAGTGAATCTGTATTGGGGTACAGGAATGAACCTTCTGC
  AACATCTTAAGATCCACAAATGAAGGGATATAAAAATAATGTCATAGGTAAGAAACACAG
  CAACAATGACTTAACCATATAAATGTGGAGGCTATCAACAAAGAATGGGCTTGAAACATT
  ATAAAAATTGACAATGATTTATTAAATATGTTTTCTCAATTGTAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000314 unedited
  ACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGCCAAGCGGCGGCAGAGCGAGGGG
  CATCAGCTACCGCCAAGTCCAGAGCCATTTCCATCCTGCAGAAGAAGCCCCGCCACCAGC
  AGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAGACATGACAGCCA
  TCATCAAAGAGATCGTTAGCAGAAACAAAAGGAGATATCAAGAGGATGGATTCGACTTAG
  ACTTGACCTATATTTATCCAAACATTATTGCTATGGGATTTCCTGCAGAAAGACTTGAAG
  GCGTATACAGGAACAATATTGATGATGTAGTAAGGTTTTTGGATTCAAAGCATAAAAACC
  ATTACAAGATATACAATCTTTGTGCTGAAAGACATTATGACACCGCCAAATTTAATTGCA
  GAGTTGCACAATATCCTTTTGAAGACCATAACCCACCACAGCTAGAACTTATCAAACCCT
  TTTGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACT
  GTAAAGCTGGAAAGGGACGAACTGGTGTAATGATATGTGCATATTTATTACATCGGGGCA
  AATTTTTAAAGGCACAAGAGGCCCTAGATTTCTATGGNGAAGTAAGGACCAGAGACAAAA
  GGGAGTAACTATTCCCAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAT
  CATCTGGATTATAGACCAGTGGCACTGGNTGTTCACAAGATGATGTTTGAAACTATNCCC
  ATGGTCAGTGGCGGAACTTNGCATCCTCAGTTNGTGGTCTGCCAGCTAAGGGTGAAGAAT
  ATNCCTCCATNCAGGACCACACGACGGGNAGACAGTCATGTCCTTTGAGTCCCTCAGCCG
  TACCTGGGTGTGTGAATCAAAGAAAAGTCTTCCCAACAGACAGAGCTAAAAGGCAAATGN
  NTACTTTGGTAAAACATNTNATACAGACAAAGAACTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000314 unedited
  ATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTACAATTGAGAAAAACATATTTAATAAATCATTGTCAATTTTTATAATGTTTCAAG
  CCCATTCTTTGTTGATAGCCTCCACATTTATATGGTTAAGTCATTGTTGCTGGGTTTCTT
  ACCTATGACATTATTTTTATATCCCTTCATTTGGGGATCTTAAAATGTTGCAAAAGGTTC
  ATTCCTGTACCCCAATACAGATTCACTTCCTTTAGCTGCCTTTTCTAGCACCAATATGCT
  TTAAAAAAAAATGCGCAAACAACAAGCAGGGACAGCGGCCAATTCCTCGAATGTCCAGAT
  TAATAACTGTAGCATGCTAAAGAAAGGGGTGTGTAAATAGCTGGAGATGGTATATGGTCC
  AGAGTCCAGCATAAAATTATTTCCTTTCTGAGCATTCCCTCCATTCCCCTAACCCGAATA
  CATGCATTAAAATGTAGCAAAACCCTTCGGAAACCTCTCTTAGCCAACTGCAAACTTATC
  TGTTGCCACAAGTGCAAAGGGGTAGGATGTGAACCAGTATATCACAAAGCTCTTTAGCCA
  CTTCAGTTGGTGACAGAACACAAAAGGAAAAAATTCCTACGTATACACATCAGTCTGTCT
  CCACTTTTTATAAAACTGGGATAAAACGGGAAAGNGCCATCTTTATTAATCCTAATTGAA
  TTTTAAATGCCCTTTTGACACAAAAGTATATACATGACACAGCTACACAACCTTTTTTCA
  ACTGGACANNCAGTGTCAAAACCCTGTGGTGTATAGGGTAAAACAAGATTGGTCAGGAAA
  GAGAATTGTTCCTAAACTGGGNATCTGACACAGTGCCTATTGCCATAAAAAAAAAGGGCC
  ATTTCAGTTATCCAAGTTATTTCATGGGGTTTATCCCTCTGAAAAAAAATCGACTTTGGA
  ATGGGATGCGGACTCACAAAGGTATCCCTGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000314
• Insert Size: 2390 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000314.3, NP_000305.3
• RefSeq Size: 3416 bp
• RefSeq ORF: 1212 bp
• Locus ID: 5728
• UniProt ID: P60484
• Cytogenetics: 10q23.31
• Domains: PTPc_motif
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Endometrial cancer, Focal adhesion, Glioma, Inositol phosphate metabolism, Melanoma, p53 signaling pathway, Pathways in cancer, Phosphatidylinositol signaling system, Prostate cancer, Small cell lung cancer, Tight junction
• Gene Summary: This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2015]
  Transcript Variant: This variant (1) encodes multiple isoforms due to the use of alternative translation initiation codons. The longest isoform (PTEN-L, PMID:23744781; also known as PTENalpha, PMID: 24768297) is derived from the use of an upstream non-AUG (CUG) start codon, while two shorter isoforms are derived from downstream AUG start codons. The most abundant isoform (PTEN), which is derived from the use of the 5'-most AUG start codon, is represented in this RefSeq. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.