PMM2 (NM_000303) Human Untagged Clone

CAT#: SC119957

PMM2 (untagged)-Human phosphomannomutase 2 (PMM2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PMM2
• Synonyms: CDG1; CDG1a; CDGS; PMI; PMI1; PMM 2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119957 sequence for NM_000303 edited (data generated by NextGen Sequencing)
  ATGGCAGCGCCTGGCCCAGCGCTCTGCCTCTTCGACGTGGATGGGACCCTCACCGCCCCG
  CGGCAGAAAATTACCAAAGAAATGGATGACTTCCTACAAAAATTGAGGCAGAAGATCAAA
  ATCGGAGTGGTAGGCGGATCGGACTTTGAGAAAGTGCAGGAGCAACTGGGAAATGATGTG
  GTTGAAAAATACGATTATGTGTTTCCAGAAAATGGCTTGGTAGCATACAAAGATGGGAAA
  CTCTTGTGTAGACAGAATATTCAAAGTCATCTGGGTGAGGCCCTAATCCAAGATTTAATC
  AACTACTGTCTGAGCTACATTGCGAAAATTAAACTCCCGAAGAAGAGGGGTACTTTCATT
  GAATTCCGAAATGGGATGTTAAACGTGTCCCCTATTGGAAGAAGCTGCAGCCAAGAAGAA
  CGCATTGAGTTCTACGAACTCGATAAAAAAGAAAATATAAGACAAAAGTTTGTAGCAGAT
  CTACGGAAAGAGTTTGCTGGAAAAGGCCTCACGTTTTCCATAGGAGGCCAGATCAGCTTT
  GATGTCTTTCCTGATGGATGGGACAAGAGATACTGTCTGCGACATGTGGAAAATGACGGT
  TATAAGACCATTTATTTCTTTGGAGACAAAACTATGCCAGGTGGCAATGACCATGAGATC
  TTCACAGACCCCAGAACCATGGGCTACTCCGTGACAGCGCCTGAGGACACGCGCAGGATC
  TGTGAACTGCTGTTCTCCTAA
  
  Clone variation with respect to NM_000303.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000303 unedited
  NGTTCAAATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGAGAACTGGG
  GACATGGCAGCGCCTGGCCCAGCGCTCTGCCTCTTCGACGTGGATGGGACCCTCACCGCC
  CCGCGGCAGAAAATTACCAAAGAAATGGATGACTTCCTACAAAAATTGAGGCAGAAGATC
  AAAATCGGAGTGGTAGGCGGATCGGACTTTGAGAAAGTGCAGGAGCAACTGGGAAATGAT
  GTGGTTGAAAAATACGATTATGTGTTTCCAGAAAATGGCTTGGTAGCATACAAAGATGGG
  AAACTCTTGTGTAGACAGAATATTCAAAGTCATCTGGGTGAGGCCCTAATCCAAGATTTA
  ATCAACTACTGTCTGAGCTACATTGCGAAAATTAAACTCCCGAAGAAGAGGGGTACTTTC
  ATTGAATTCCGAAATGGGATGTTAAACGTGTCCCCTATTGGAAGAAGCTGCAGCCAAGAA
  GAACGCATTGAGTTCTACGAACTCGATAAAAAAGAAAATATAAGACAAAAGTTTGTAGCA
  GATCTACGGAAAGAGTTTGCTGGAAAAGGCCTCACGTTTTCCATAGGAGGCCAGATCAGC
  TTTGATGTCTTTCCTGATGGATGGGACAAGAGATACTGTCTGCGACATGTGGAAAATGAC
  GGTTATAAGACCATTTATTTCTTTGGAGACAAAACTATGCCAGGTGGCAATGAACCATGA
  GATCTTCACAGACCCCAGAACCATGGGCTACTCCGTGACAGCGCCTNGAGACACGCGCAG
  NATCTGTGAACTGCTGTTCTCCTACGTGNGAACGGNAGGGGCNNGGGTCCCCGCTGACAG
  CCAGCATAGGGCATTCGTGGCCAGAGCCGAGGGTNCTCCCACACGTGCTCACCCACCGCA
  GCCTAGCAGCTCTGATGCTATGCAGCATGTGCAGTCTGGACTCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000303 unedited
  GCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAACAAAAAAATCACTTTAAGGGGCTCCTTT
  TCACTTTGGAAGGGCTTTACCGTTTTTTTCCACAAGGAAAAATCCCCCTGGATGGCTGGG
  CCCTGGGAATCACTGGGAAAAACTGGCTGTTTTCCCCCCCCCGGATCCGGGGCTTAAACA
  GTTACAACCCCAAGTGGCTGGGAGGTTTGGAGGGAACCAGGTTTACCTGGGATAAAACCC
  CCTGGCTTGGGGCTGGGAGGCAAGAATGCAGGGCACCCTGGGCTCCCCTGGCCCCAAACT
  TAGGGCGGCAAGGGCCCAGGGGGGGCCCCCCAACCCCACCCCCAAACAAGGGGGGGGGTA
  TGGCTCAATGTTTCCCCCTCCTGAAAAGGGACCCATGAACCCTTTCCTAAGAAAGTTCTT
  TTCCACCGGGTTTTAACTGAAACCCGGGATCCTTCCATTTTCACAAAAAACGTGCAAAAA
  ATGAAAAAAGGGGGGGACCCGGCCCTAGCTGTAGCTGCCCTCACCCCCCTCCGGGGTAAA
  ATCTGGGTAAAGGCCTGGGTGAATGCAAATAGGAAAACAACTCCTCTTACTGGTCTCTGG
  CTGGGCTCTCGCTCCATCCCTGAAAAACAAAGGCGACCGCCTCCAAGAACCCAGCCCGGG
  CCACCAAAAGTCCTTTTGGAGAAACTGACATTCCCCTTTGTTTCTCTGAACTGGGAAAAA
  CCGGCCCAGGGAAGGATGTTTTGCCACCCCACCTCTCACACACTTGTGGTTTCACTACCT
  CCGCTCGGAACACAAAAACACCCGCTTTTTTATGTGAGAAACCAAACGTCCAAACCGCCA
  CCGAGGGAACTCCTAGGTTGTCCCTTGGCCCCTTGTCCAAAGCCTACCCAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000303
• Insert Size: 2640 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000303.1, NP_000294.1
• RefSeq Size: 2302 bp
• RefSeq ORF: 741 bp
• Locus ID: 5373
• UniProt ID: O15305
• Cytogenetics: 16p13.2
• Domains: PMM
• Protein Families: Druggable Genome
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Fructose and mannose metabolism, Metabolic pathways
• Gene Summary: The protein encoded by this gene catalyzes the isomerization of mannose 6-phosphate to mannose 1-phosphate, which is a precursor to GDP-mannose necessary for the synthesis of dolichol-P-oligosaccharides. Mutations in this gene have been shown to cause defects in glycoprotein biosynthesis, which manifests as carbohydrate-deficient glycoprotein syndrome type I. [provided by RefSeq, Jul 2008]