Glucose 6 Phosphate Dehydrogenase (G6PD) (NM_000402) Human Untagged Clone

CAT#: SC119906

G6PD (untagged)-Human glucose-6-phosphate dehydrogenase (G6PD), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Glucose 6 Phosphate Dehydrogenase
• Synonyms: G6PD1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000402 edited
  ATGGGCCGGCGGGGCTCAGCCCCCGGAAACGGTCGTACACTTCGGGGCTGCGAGCGCGGA
  GGGCGACGACGACGAAGCGCAGACAGCGTCATGGCAGAGCAGGTGGCCCTGAGCCGGACC
  CAGGTGTGCGGGATCCTGCGGGAAGAGCTTTTCCAGGGCGATGCCTTCCATCAGTCGGAT
  ACACACATATTCATCATCATGGGTGCATCGGGTGACCTGGCCAAGAAGAAGATCTACCCC
  ACCATCTGGTGGCTGTTCCGGGATGGCCTTCTGCCCGAAAACACCTTCATCGTGGGCTAT
  GCCCGTTCCCGCCTCACAGTGGCTGACATCCGCAAACAGAGTGAGCCCTTCTTCAAGGCC
  ACCCCAGAGGAGAAGCTCAAGCTGGAGGACTTCTTTGCCCGCAACTCCTATGTGGCTGGC
  CAGTACGATGATGCAGCCTCCTACCAGCGCCTCAACAGCCACATGAATGCCCTCCACCTG
  GGGTCACAGGCCAACCGCCTCTTCTACCTGGCCTTGCCCCCGACCGTCTACGAGGCCGTC
  ACCAAGAACATTCACGAGTCCTGCATGAGCCAGATAGGCTGGAACCGCATCATCGTGGAG
  AAGCCCTTCGGGAGGGACCTGCAGAGCTCTGACCGGCTGTCCAACCACATCTCCTCCCTG
  TTCCGTGAGGACCAGATCTACCGCATCGACCACTACCTGGGCAAGGAGATGGTGCAGAAC
  CTCATGGTGCTGAGATTTGCCAACAGGATCTTCGGCCCCATCTGGAACCGGGACAACATC
  GCCTGCGTTATCCTCACCTTCAAGGAGCCCTTTGGCACTGAGGGTCGCGGGGGCTATTTC
  GATGAATTTGGGATCATCCGGGACGTGATGCAGAACCACCTACTGCAGATGCTGTGTCTG
  GTGGCCATGGAGAAGCCCGCCTCCACCAACTCAGATGACGTCCGTGATGAGAAGGTCAAG
  GTGTTGAAATGCATCTCAGAGGTGCAGGCCAACAATGTGGTCCTGGGCCAGTACGTGGGG
  AACCCCGATGGAGAGGGCGAGGCCACCAAAGGGTACCTGGACGACCCCACGGTGCCCCGC
  GGGTCCACCACCGCCACTTTTGCAGCCGTCGTCCTCTATGTGGAGAATGAGAGGTGGGAT
  GGGGTGCCCTTCATCCTGCGCTGCGGCAAGGCCCTGAACGAGCGCAAGGCCGAGGTGAGG
  CTGCAGTTCCATGATGTGGCCGGCGACATCTTCCACCAGCAGTGCAAGCGCAACGAGCTG
  GTGATCCGCGTGCAGCCCAACGAGGCCGTGTACACCAAGATGATGACCAAGAAGCCGGGC
  ATGTTCTTCAACCCCGAGGAGTCGGAGCTGGACCTGACCTACGGCAACAGATACAAGAAC
  GTGAAGCTCCCTGACGCCTACGAGCGCCTCATCCTGGACGTCTTCTGCGGGAGCCAGATG
  CACTTCGTGCGCAGCGACGAGCTCCGTGAGGCCTGGCGTATTTTCACCCCACTGCTGCAC
  CAGATTGAGCTGGAGAAGCCCAAGCCCATCCCCTATATTTATGGCAGCCGAGGCCCCACG
  GAGGCAGACGAGCTGATGAAGAGAGTGGGTTTCCAGTATGAGGGCACCTACAAGTGGGTG
  AACCCCCACAAGCTCTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000402 unedited
  AGCATATTTGTATACGACTCACTATTAGGGCGGCCGCGAATTCGCACGAGGCCGCCCCCG
  CCGATTAAATGGGCCGGCGGGGCTCAGCCCCCGGAAACGGTCGTACACTTCGGGGCTGCG
  AGCGCGGAGGGCGACGACGACGAAGCGCAGACAGCGTCATGGCAGAGCAGGTGGCCCTGA
  GCCGGACCCAGGTGTGCGGGATCCTGCGGGAAGAGCTTTTCCAGGGCGATGCCTTCCATC
  AGTCGGATACACACATATTCATCATCATGGGTGCATCGGGTGACCTGGCCAAGAAGAAGA
  TCTACCCCACCATCTGGTGGCTGTTCCGGGATGGCCTTCTGCCCGAAAACACCTTCATCG
  TGGGCTATGCCCGTTCCCGCCTCACAGTGGCTGACATCCGCAAACAGAGTGAGCCCTTCT
  TCAAGGCCACCCCAGAGGAGAAGCTCAAGCTGGAGGACTTCTTTGCCCGCAACTCCTATG
  TGGCTGGCCAGTACGATGATGCAGCCTCCTACCAGCGCCTCAACAGCCACATGAATGCCC
  TCCACCTGGGGTCACAGGCCAACCGCCTCTTCTACCTGGCCTTGCCCCCGACCGTCTACG
  AGGCCGTCACCAAGAACATTCACGAGTCCTGCATGAGCCAGATAGGCTGGAACCGCATCA
  TCGTGGAGAAGCCCTTCGGGAGGGACCTGCAGAGCTCTGACCGGCTGTCCAACCACATCT
  CCTCCCTGTTCCGTGAGGACCAGATCTACCGCATCGACCACTACCTGGGCAAGGAGATGG
  TGCAGACCCTCATGGTGCTGAGATTTGCCCACAGGATCTTTCGCCCCATCTGGACCGGGA
  CACATCGNCTGCGTTATCCTCACCTTCAGGNAGCCCTTTTGCACTGAGGGTCGCGGNGGC
  TATTTCGATGAATTTGGGAAATTCCGGACGTGATGCAGAACACCTACTGCAGAGCTGNGT
  CTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000402 unedited
  CCCCCCCCAATTAGTGTGCNCNCGCGGCCCGCTATTCTAGGACTCGGTTTTTTTTTTTTT
  TTTTTGGCTGTTTGCGGATTTAATGGCAGGGCATTGAGGTTGGGAGGGGTCCCAGCTGCT
  GCGTCTGCTTTTCTTATAGCAGAGAGGCTGCCTACGGGTGGCACGGGGTGGCCATGGAGT
  GCAGAGTTGGTGGGACAGGGGACATCCAGGGGGCTCGAGATGTTGCTGGTGACAAGGAAT
  GTCAAGTGGCACTGAGGCTGGCGGGCAAGGCCACAGGCAGATTCTCTCACGTGGGTGCTC
  GCCCCTTTCCTCCCCCTTGTCCCTCCCTCCCACCCTGGCCCCACTCAGGAGTGAGACCCA
  GTGGCCAATAAGCTCTGGGACAGACGAATGGGCGCCCTCCTCCTTCCTTCTGTTGGGCTG
  GAGTGAGTGGAGGAGGTGACTCAGCTCCTGGGCTCAGGCAGGGTCTGGAGGGGCCAGGAT
  GGTCTCGAGTGCTTGGCAGCTGAGGAATGTAGCTGGGCTCGGGTAGTAGCAGCAGCGAGG
  GGCGGGCCAGGGTGGCCAGAGCCCGGGGCCAGGAATGTGCAGCTGAGGTCAATGGTCCCG
  GAGTCCTCCCGACTCGGGGTCGGGCGGCGGGAAGGAGGGTGGCCGTGGCGGGGGTGGAGG
  TGGGTGCCCAGGGCTCACAGCTTGTGGGGGGTCACCCACTTGTAGGTGCCCTCATACTGG
  AAACCACTCTCTTCATCAGCTCGTCTGCCTCCGTGGGGCCTCCGCTGCCATAATATAGGG
  GATGGGCCTTGGGCTTTTCCAGCTCAATCTGGGCCCCCCCGGGGTGAAAATACGCCAGGC
  CTTACGGAACTTGTCGCTGTGCCCCAAGGCATTTGGGTTCCTGAGAACACTTCCAGAATG
  AGCGCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000402
• Insert Size: 2400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000402.2, NP_000393.2
• RefSeq Size: 2650 bp
• RefSeq ORF: 2406 bp
• Locus ID: 2539
• UniProt ID: P11413
• Cytogenetics: Xq28
• Domains: G6PD
• Protein Families: Druggable Genome
• Protein Pathways: Glutathione metabolism, Metabolic pathways, Pentose phosphate pathway
• Gene Summary: This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). This full-length 545 aa form has been reported to be inactive, but may be processed to the smaller (515 aa) active form (PMID:8466644).