EGR2 (NM_000399) Human Untagged Clone

CAT#: SC119904

EGR2 (untagged)-Human early growth response 2 (EGR2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EGR2
• Synonyms: AT591; CHN1; CMT1D; CMT4E; KROX20
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000399 edited
  ATGATGACCGCCAAGGCCGTAGACAAAATCCCAGTAACTCTCAGTGGTTTTGTGCACCAG
  CTGTCTGACAACATCTACCCGGTGGAGGACCTCGCCGCCACGTCGGTGACCATCTTTCCC
  AATGCCGAACTGGGAGGCCCCTTTGACCAGATGAACGGAGTGGCCGGAGATGGCATGATC
  AACATTGACATGACTGGAGAGAAGAGGTCGTTGGATCTCCCATATCCCAGCAGCTTTGCT
  CCCGTCTCTGCACCTAGAAACCAGACCTTCACTTACATGGGCAAGTTCTCCATTGACCCT
  CAGTACCCTGGTGCCAGCTGCTACCCAGAAGGCATAATCAATATTGTGAGTGCAGGCATC
  TTGCAAGGGGTCACTTCCCCAGCTTCAACCACAGCCTCATCCAGCGTCACCTCTGCCTCC
  CCCAACCCACTGGCCACAGGACCCCTGGGTGTGTGCACCATGTCCCAGACCCAGCCTGAC
  CTGGACCACCTGTACTCTCCGCCACCGCCTCCTCCTCCTTATTCTGGCTGTGCAGGAGAC
  CTCTACCAGGACCCTTCTGCGTTCCTGTCAGCAGCCACCACCTCCACCTCTTCCTCTCTG
  GCCTACCCACCACCTCCTTCCTATCCATCCCCCAAGCCAGCCACGGACCCAGGTCTCTTC
  CCAATGATCCCAGACTATCCTGGATTCTTTCCATCTCAGTGCCAGAGAGACCTACATGGT
  ACAGCTGGCCCAGACCGTAAGCCCTTTCCCTGCCCACTGGACACCCTGCGGGTGCCCCCT
  CCACTCACTCCACTCTCTACAATCCGTAACTTTACCCTGGGGGGCCCCAGTGCTGGGGTG
  ACCGGACCAGGGGCCAGTGGAGGCAGCGAGGGACCCCGGCTGCCTGGTAGCAGCTCAGCA
  GCAGCAGCAGCCGCCGCCGCCGCCGCCTATAACCCACACCACCTGCCACTGCGGCCCATT
  CTGAGGCCTCGCAAGTACCCCAACAGACCCAGCAAGACGCCGGTGCACGAGAGGCCCTAC
  CCGTGCCCAGCAGAAGGCTGCGACCGGCGGTTCTCCCGCTCTGACGAGCTGACACGGCAC
  ATCCGAATCCACACTGGGCATAAGCCCTTCCAGTGTCGGATCTGCATGCGCAACTTCAGC
  CGCAGTGACCACCTCACCACCCATATCCGCACCCACACCGGTGAGAAGCCCTTCGCCTGT
  GACTACTGTGGCCGAAAGTTTGCCCGGAGTGATGAGAGGAAGCGCCACACCAAGATCCAC
  CTGAGACAGAAAGAGCGGAAAAGCAGTGCCCCCTCTGCATCGGTGCCAGCCCCCTCTACA
  GCCTCCTGCTCTGGGGGCGTGCAGCCTGGGGGTACCCTGTGCAGCAGTAACAGCAGCAGT
  CTTGGCGGAGGGCCGCTCGCCCCTTGCTCCTCTCGGACCCGGACACCTTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000399 unedited
  NGGTTCGAATTATGTATACGACTCACTATAGGGNNCGGCCGCGNAATTCGGCACGAGGGA
  CTCACTGACTGTTATAATAACACTACACCAGCAACTCCTGGCTTCCCAGCAGCCGGAACA
  CAGACAGGAGAGAGTCAGTGGCAAATAGACATTTTTCTTATTTCTTAAAAAACAGCAACT
  TGTTTGCTACTTTTATTTCTGTTGATTTTTTTTTCTTGGTGTGTGTGGTGGTTGTTTTTA
  AGTGTGGAGGGCAAAAGGAGATACCATCCCAGGCTCAGTCCAACCCCTCTCCAAAACGGC
  TTTTCTGACACTCCAGGTAGCGAGGGAGTTGGGTCTCCAGGTTGTGCGAGGAGCAAATGA
  TGACCGCCAAGGCCGTAGACAAAATCCCAGTAACTCTCAGTGGTTTTGTGCACCAGCTGT
  CTGACAACATCTACCCGGTGGAGGACCTCGCCGCCACGTCGGCGACCATCTTTCCCAATG
  CCGAACTGGGAGGCCCCTTTGACCAGATGAACGGAGTGGCCGGAGATGGCATGATCAACA
  TTGACATGACTGGAGAGAAGAGGTCGTTGGATCTCCCATATCCCAGCAGCTTTGCTCCCG
  CCCTCGCACCTAGAAACCAGACCTCCACTACATGCGCAAGCCCTCCATGACCCTCAATAC
  CCTGGCGCCAGCCGCTACCCAGAACGCCCCACCCCACCGTGAGCGCACGCATCTTGCACA
  GGGTCACTTCCCCACTCTCAACCCACAGCCCCATCAACCGCACCTCCCACCCCCCCACAC
  CCATGCACCCAGGACCCCCGCCCCCGCTCTTCTCCCCCCACAACCCCTTGTCCTCTTACA
  AATCTAACTTTCCGTCACCTATTCTTCTCCTTCCTGCATTTAACAAATCATTCATCTACC
  TCTCCGTCTCCTCTAATCACTCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000399 unedited
  GGCCGCATCTAGATCGGTTTTTTTTTTTTTTTTTTATGTTGAAAATATTTATTTACACTA
  TAGTCACAAACCATCCATTATCTGAACTTCAGTTAGTGGTTTTGCGTTAGAATAGATTTA
  TTTTTTCACAACTATTAAGAGCTAATGACCAAACAAATCAGCTCCGGTAACATTATGTAC
  ATTCTTATCTGAAATTTGACTACAAAGGTAACACTTTTAAGTCACAAAACAAAGCATACA
  AAAATATACTTTCTAAAAAAAATTCCAAATATTAATAGGCAGAAATATACAGCCATACTA
  AACTCAGGGAGTGATTTTTTTCTCCATAATAAGGCAACCCATTTACATGCAGACCTTGTA
  ACATTGTCTACATCACACAAGGCACCAAGGACACTTCCAACACAATTGCATGCATCCTAG
  TTTCAGAGAATATATGTACATCCCCCTTAAATAAGTTAACCTCTGAGATCATAAATGCTT
  TACATTTTTTCCCAAAGATTCAGAATTTAGAGAACAGTGTACATCAAAAATACACAAAAT
  CTGAGTGGATATACACTGAAAAAATAGCCTACATTTCTCAAACAACTCGAAAAGGAGCAG
  ATATTGCTATGTTCCTCCCATCACATTGCACTTCTGTTCTCTGAGTTTATAATACATATT
  GCTTCAAGGGTTGAGACAACTAGATATGCTCTGATTCACCATTTTAGGGCCACAGAGAGT
  GGCATAAAGGCTATACTTCACTCACTGTACAATGTCCCCCAAATCAGTCCCCAGCCCATA
  AGNGCACATCAGTTTTGCTTGTCTTTTTGCTGNCCCACCTGAAGAATCAGCATCTCCCAC
  CTATTGTAGAAACGCTTATATCACTCTGACTCCAGGTCAAAACCTGGATGGGCAAATTAT
  GGTAGTGGGTACAAAACTATTAAAAACCTTTTAAGCAGGTAGAACTCACCTCTTGCCAGG
  GTCCCACACAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000399
• Insert Size: 2840 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: no
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000399.2, NP_000390.2
• RefSeq Size: 2984 bp
• RefSeq ORF: 1431 bp
• Locus ID: 1959
• UniProt ID: P11161
• Cytogenetics: 10q21.3
• Domains: zf-C2H2
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: The protein encoded by this gene is a transcription factor with three tandem C2H2-type zinc fingers. Defects in this gene are associated with Charcot-Marie-Tooth disease type 1D (CMT1D), Charcot-Marie-Tooth disease type 4E (CMT4E), and with Dejerine-Sottas syndrome (DSS). Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1, 2, and 3 all encode the same isoform.