CD31 (PECAM1) (NM_000442) Human Untagged Clone

CAT#: SC119894

PECAM1 (untagged)-Human platelet/endothelial cell adhesion molecule (PECAM1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD31
• Synonyms: CD31; CD31/EndoCAM; endoCAM; GPIIA'; PECA1; PECAM-1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000442 edited
  ATGCAGCCGAGGTGGGCCCAAGGGGCCACGATGTGGCTTGGAGTCCTGCTGACCCTTCTG
  CTCTGTTCAAGCCTTGAGGGTCAAGAAAACTCTTTCACAATCAACAGTGTTGACATGAAG
  AGCCTGCCGGACTGGACGGTGCAAAATGGGAAGAACCTGACCCTGCAGTGCTTCGCGGAT
  GTCAGCACCACCTCTCACGTCAAGCCTCAGCACCAGATGCTGTTCTATAAGGATGACGTG
  CTGTTTTACAACATCTCCTCCATGAAGAGCACAGAGAGTTATTTTATTCCTGAAGTCCGG
  ATCTATGACTCAGGGACATATAAATGTACTGTGATTGTGAACAACAAAGAGAAAACCACT
  GCAGAGTACCAGGTGTTGGTGGAAGGAGTGCCCAGTCCCAGGGTGACACTGGACAAGAAA
  GAGGCCATCCAAGGTGGGATCGTGAGGGTCAACTGTTCTGTCCCAGAGGAAAAGGCCCCA
  ATACACTTCACAATTGAAAAACTTGAACTAAATGAAAAAATGGTCAAGCTGAAAAGAGAG
  AAGAATTCTCGAGACCAGAATTTTGTGATACTGGAATTCCCCGTTGAGGAACAGGACCGC
  GTTTTATCCTTCCGATGTCAAGCTAGGATCATTTCTGGGATCCATATGCAGACCTCAGAA
  TCTACCAAGAGTGAACTGGTCACCGTGACGGAATCCTTCTCTACACCCAAGTTCCACATC
  AGCCCCACCGGAATGATCATGGAAGGAGCTCAGCTCCACATTAAGTGCACCATTCAAGTG
  ACTCACCTGGCCCAGGAGTTTCCAGAAATCATAATTCAGAAGGACAAGGCGATTGTGGCC
  CACAACAGACATGGCAACAAGGCTGTGTACTCAGTCATGGCCATGGTGGAGCACAGTGGC
  AACTACACGTGCAAAGTGGAGTCCAGCCGCATATCCAAGGTCAGCAGCATCGTGGTCAAC
  ATAACAGAACTATTTTCCAAGCCCGAACTGGAATCTTCCTTCACACATCTGGACCAAGGT
  GAAAGACTGAACCTGTCCTGCTCCATCCCAGGAGCACCTCCAGCCAACTTCACCATCCAG
  AAGGAAGATACGATTGTGTCACAGACTCAAGATTTCACCAAGATAGCCTCAAAGTCGGAC
  AGTGGGACGTATATCTGCACTGCAGGTATTGACAAAGTGGTCAAGAAAAGCAACACAGTC
  CAGATAGTCGTATGTGAAATGCTCTCCCAGCCCAGGATTTCTTATGATGCCCAGTTTGAG
  GTCATAAAAGGACAGACCATCGAAGTCCGTTGCGAATCGATCAGTGGAACTTTGCCTATT
  TCTTACCAACTTTTAAAAACAAGTAAAGTTTTGGAGAATAGTACCAAGAACTCAAATGAT
  CCTGCGGTATTCAAAGACAACCCCACTGAAGACGTCGAATACCAGTGTGTTGCAGATAAT
  TGCCATTCCCACGCCAAAATGTTAAGTGAGGTTCTGAGGGTGAAGGTGATAGCCCCGGTG
  GATGAGGTCCAGATTTCTATCCTGTCAAGTAAGGTGGTGGAGTCTGGAGAGGACATTGTG
  CTGCAATGTGCTGTGAATGAAGGATCTGGTCCCATCACCTATAAGTTTTACAGAGAAAAA
  GAGGGCAAACCCTTCTATCAAATGACCTCAAATGCCACCCAGGCATTTTGGACCAAGCAG
  AAGGCTAACAAGGAACAGGAGGGAGAGTATTACTGCACAGCCTTCAACAGAGCCAACCAC
  GCCTCCAGTGTCCCCAGAAGCAAAATACTGACAGTCAGAGTCATTCTTGCCCCATGGAAG
  AAAGGACTTATTGCAGTGGTTATCATCGGAGTGATCATTGCTCTCTTGATCATTGCGGCC
  AAATGTTATTTTCTGAGGAAAGCCAAGGCCAAGCAGATGCCAGTGGAAATGTCCAGGCCA
  GCAGTACCACTTCTGAACTCCAACAACGAGAAAATGTCAGATCCCAATATGGAAGCTAAC
  AGTCATTACGGTCACAATGACGATGTCGGAAACCATGCAATGAAACCAATAAATGATAAT
  AAAGAGCCTCTGAACTCAGACGTGCAGTACACGGAAGTTCAAGTGTCCTCAGCTGAGTCT
  CACAAAGATCTAGGAAAGAAGGACACAGAGACAGTGTACAGTGAAGTCCGGAAAGCTGTC
  CCTGATGCCGTGGAAAGCAGATACTCTAGAACGGAAGGCTCCCTTGATGGAACTTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000442 unedited
  TATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGCCATTACCTGACCAGC
  GCCACAGCCGGTCTCTCTGCAGGCGCCGGGAGAAGTGACCAGAGCAATTTCTGCTTTTCA
  CAGGGCGGGTTTCTCAATGGTGACTTGTGGGCAGTGCCTTCTGCTGAGCGAGTCATGGCC
  CGAAGGCAGAACTAACTGTGCCTGCAGTCTTCACTCTCAGGATGCAGCCGAGGTGGGCCC
  AAGGGGCCACGATGTGGCTTGGAGTCCTGCTGACCCTTCTGCTCTGTTCAAGCCTTGAGG
  GTCAAGAAAACTCTTTCACAATCAACAGTGTTGACATGAAGAGCCTGCCGGACTGGACGG
  TGCAAAATGGGAAGAACCTGACCCTGCAGTGCTTCGCGGATGTCAGCACCACCTCTCACG
  TCAAGCCTCAGCACCAGATGCTGTTCTATAAGGATGACGTGCTGTTTTACAACATCTCCT
  CCATGAAGAGCACAGAGAGTTATTTTATTCCTGAAGTCCGGATCTATGACTCAGGGACAT
  ATAAATGTACTGTGATTGTGAACAACAAAGAGAAAACCACTGCAGAGTACCAGGTGTTGG
  TGGAAGGAGTGCCCANTCCCAGGGTGACACTGGACAAGAAAAGAGCCATCCAAGGTGGGA
  TCGTGAGGGTCACCTGTTCTGTCCCAAGGAAAGGCCCCATACACTTCACATTGAAAACTT
  GACTAAATGAAAAATGTCAAGCTGAAAGAGAGAGAATTCTCGAGACAGATTTTGTGAACT
  GGAATTCCCGTTGAGGACAAGACCGCGTTTATCCTTCGTGTCAGCTAGGACATTTNTGGA
  TCCTATGCAGCCTAAAATTACCAAGAGGACTGTCACGGGACGGATCCTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000442 unedited
  GACAAGAGTCAAATTTATTTAATACAACATCCACGAGGTCCCTGCAGCTGTGTCACTGAG
  GCAAACAGGAAAGTGATTTTGGCTAGGCGTGGTTCNTCATCTGTGAAATTCCACAGCGCA
  ATGACAGCAGCCTCTCTCCCACCCACTCAAGACACTGTCAGGAATGTCTTAAGACCTCAG
  GAGACCACTTCTTTAGCAAGCAATTTTGTTTTTTGTTTTTTTTGAGATGGATTCTCACTC
  TGTCACTCAGGCTGGAGTGCAGTGGCGCGATCTCCGCTCACTACAACCTCCGTTTCCTGG
  GTTCAAGCGATAATCTCACCTCAGCCTCTTGAGTAGCTGATACTACAGGCATGCGCCACC
  ACGCCCGGCTAATTCTTGTATTTTTAGTAGAGACAGGGTTTCATCAGGCTGGTCTCAAAC
  TCCTGACCTCAGGTGATCCGCCCACCTCAGCCTCCTGAAGTGCTGGGATTACAGGCGTGA
  GCCACCTTGCCTGCCCTGGCAAGGAATACATTTTTAAAAATTAGTAAGAAACATACACAT
  TTCAAGTTTTCAATTAAGAATAATATTTGCTGATGGCACCATCTTCCTGTCTTTCAGCCT
  TCAGCCCGGTCGAGGAAAAGAGAAAGAGTGTATACACAAACAGTTGAAGAACATCTGCGC
  TTGGGTCACCTTCATTTTCTGCGCCGTGCTGCGCCTGAACGACCGGTGTCCTCATGGTGG
  CATTTCATAGGCGGGGCTCCCAAGTAATTCTGGTTTTCCATTCGTGAGGGGCCCGGCCCT
  AAAGGGGGCCCGGGGCCGTTCGTCTGCGCTCCCCTGTTCCTTCCCACGCCCAGGTTCTCC
  CCCAACTTCCATCCCCCCGAGGATCTTGTCATTATTCCACACCCGGCTTTACCCCGCCAG
  GTTTTTGAGGATCGCGTTATAAACAATCTGCTTTCCCTTAGCCCCTAGAAGGGCTCACTC
  TCCCACACCCTTCCCTAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000442
• Insert Size: 3700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000442.2, NP_000433.2
• RefSeq Size: 3189 bp
• RefSeq ORF: 2217 bp
• Locus ID: 5175
• UniProt ID: P16284
• Cytogenetics: 17q23.3
• Domains: ig, IGc2, IG
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Leukocyte transendothelial migration
• Gene Summary: The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]