Hydroxysteroid (17 beta) Dehydrogenase 4 (HSD17B4) (NM_000414) Human Untagged Clone

CAT#: SC119874

HSD17B4 (untagged)-Human hydroxysteroid (17-beta) dehydrogenase 4 (HSD17B4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Hydroxysteroid (17 beta) Dehydrogenase 4
• Synonyms: DBP; MFE-2; MFP-2; MPF-2; PRLTS1; SDR8C1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119874 sequence for NM_000414 edited (data generated by NextGen Sequencing)
  ATGGGCTCACCGCTGAGGTTCGACGGGCGGGTGGTACTGGTCACCGGCGCGGGGGCAGGA
  TTGGGCCGAGCCTATGCCCTGGCTTTTGCAGAAAGAGGAGCGTTAGTTGTTGTGAATGAT
  TTGGGAGGGGACTTCAAAGGAGTTGGTAAAGGCTCCTTAGCTGCTGATAAGGTTGTTGAA
  GAAATAAGAAGGAGAGGTGGAAAAGCAGTGGCCAACTATGATTCAGTGGAAGAAGGAGAG
  AAGGTTGTGAAGACAGCCCTGGATGCTTTTGGAAGAATAGATGTTGTGGTCAACAATGCT
  GGAATTCTGAGGGATCGTTCCTTTGCTAGGATAAGTGATGAAGACTGGGATATAATCCAC
  AGAGTTCATTTGCGGGGTTCATTCCAAGTGACACGGGCAGCATGGGAACACATGAAGAAA
  CAGAAGTATGGAAGGATTATTATGACTTCATCAGCTTCAGGAATATATGGCAACTTTGGC
  CAGGCCAATTATAGTGCTGCAAAGTTGGGTCTTCTGGGCCTTGCAAATTCTCTTGCAATT
  GAAGGCAGGAAAAGCAACATTCATTGTAACACCATTGCTCCTAATGCGGGATCACGGATG
  ACTCAGACAGTTATGCCTGAAGATCTTGTGGAAGCCCTGAAGCCAGAGTATGTGGCACCT
  CTTGTCCTTTGGCTTTGTCACGAGAGTTGTGAGGAGAATGGTGGCTTGTTTGAGGTTGGA
  GCAGGATGGATTGGAAAATTACGCTGGGAGCGGACTCTTGGAGCTATTGTAAGACAAAAG
  AATCACCCAATGACTCCTGAGGCAGTCAAGGCTAACTGGAAGAAGATCTGTGACTTTGAG
  AATGCCAGCAAGCCTCAGAGTATCCAAGAATCAACTGGCAGTATAATTGAAGTTCTGAGT
  AAAATAGATTCAGAAGGAGGAGTTTCAGCAAATCATACTAGTCGTGCAACGTCTACAGCA
  ACATCAGGATTTGCTGGAGCTATTGGCCAGAAACTCCCTCCATTTTCTTATGCTTATACG
  GAACTGGAAGCTATTATGTATGCCCTTGGAGTGGGAGCGTCAATCAAGGATCCAAAAGAT
  TTGAAATTTATTTATGAAGGAAGTTCTGATTTCTCCTGTTTGCCCACCTTCGGAGTTATC
  ATAGGTCAGAAATCTATGATGGGTGGAGGATTAGCAGAAATTCCTGGACTTTCAATCAAC
  TTTGCAAAGGTTCTTCATGGAGAGCAGTACTTAGAGTTATATAAACCACTTCCCAGAGCA
  GGAAAATTAAAATGTGAAGCAGTTGTTGCTGATGTCCTAGATAAAGGATCCGGTGTAGTG
  ATTATTATGGATGTCTATTCTTATTCTGAGAAGGAACTTATATGCCACAATCAGTTCTCT
  CTCTTTCTTGTTGGCTCTGGAGGCTTTGGTGGAAAACGGACATCAGACAAAGTCAAGGTA
  GCTGTAGCCATACCTAATAGACCTCCTGATGCTGTACTTACAGATACCACCTCTCTTAAT
  CAGGCTGCTTTGTACCGCCTCAGTGGAGACTGGAATCCCTTACACATTGATCCTAACTTT
  GCTAGTCTAGCAGGTTTTGACAAGCCCATATTACATGGATTATGTACATTTGGATTTTCT
  GCCAGGCGTGTGTTACAGCAGTTTGCAGATAATGATGTGTCAAGATTCAAGGCAATTAAG
  GCTCGTTTTGCAAAACCAGTATATCCAGGACAAACTCTACAAACTGAGATGTGGAAGGAA
  GGAAACAGAATTCATTTTCAAACCAAGGTCCAAGAAACTGGAGACATTGTCATTTCAAAT
  GCATATGTGGATCTTGCACCAACATCTGGTACTTCAGCTAAGACACCCTCTGAGGGCGGG
  AAGCTTCAGAGTACCTTTGTATTTGAGGAAATAGGACGCCGCCTAAAGGATATTGGGCCT
  GAGGTGGTGAAGAAAGTAAATGCTGTATTTGAGTGGCATATAACCAAAGGCGGAAATATT
  GGGGCTAAGTGGACTATTGACCTGAAAAGTGGTTCTGGAAAAGTGTACCAAGGCCCTGCA
  AAAGGTGCTGCTGATACAACAATCATACTTTCAGATGAAGATTTCATGGAGGTGGTCCTG
  GGCAAGCTTGACCCTCAGAAGGCATTCTTTAGTGGCAGGCTGAAGGCCAGAGGGAACATC
  ATGCTGAGCCAGAAACTTCAGATGATTCTTAAAGACTACGCCAAGCTCTGA
  
  Clone variation with respect to NM_000414.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000414 unedited
  NGGTCAGCATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCAGGCTTGTTCG
  TGTGTGTGTCGTTGCAGGCCTTATTCATGGGCTCACCGCTGAGGTTCGACGGGCGGGTGG
  TACTGGTCACCGGCGCGGGGGCAGGATTGGGCCGAGCCTATGCCCTGGCTTTTGCAGAAA
  GAGGAGCGTTAGTTGTTGTGAATGATTTGGGAGGGGACTTCAAAGGAGTTGGTAAAGGCT
  CCTTAGCTGCTGATAAGGTTGTTGAAGAAATAAGAAGGAGAGGTGGAAAAGCAGTGGCCA
  ACTATGATTCAGTGGAAGAAGGAGAGAAGGTTGTGAAGACAGCCCTGGATGCTTTTGGAA
  GAATAGATGTTGTGGTCAACAATGCTGGAATTCTGAGGGATCGTTCCTTTGCTAGGATAA
  GTGATGAAGACTGGGATATAATCCACAGAGTTCATTTGCGGGGTTCATTCCAAGTGACAC
  GGGCAGCATGGGAACACATGAAGAAACAGAAGTATGGAAGGATTATTATGACTTCATCAG
  CTTCAGGAATATATGGCAACTTTGGCCAGGCCAATTATAGTGCTGCAAAGTTGGGTCTTC
  TGGGCCTTGCAAATTCTCTTGCAATTGAAGGCAGGAAAAGCAACATTCATTGTAACACCA
  TTGCTCCTAATGCGGGATCACGGATGACTCAGACAGTTATGCCTGAAGATCTTGTGGAAG
  CCCTGAAGCCAGAGTATGTGGCACCTCTTGTCCTTTGGCTNTGTCACGAGAAGTGTGAGG
  AGAATGGTGCCTTGTTTGAGGTTGGAGCCAGATGGATTGGAAATTACGCTNGGAGCGACT
  CTTGNAGCTATTGTAGACAAAGATCACCCCATGACTCCTGAGCAGTCAAGGCTACTGGAG
  AA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000414 unedited
  GAACGCGGCCGCAATCTATAGTCGAGTTTTTTTTTTTTTTTTTTTTTAAAGTATCAAAGG
  CCTTTATTGATTTAATTTTCCTTTCAACTTATCAATGCTATCACAAATTCTAAGGAAACA
  GGACTTGGGCAAAATTTTTTATTATGAAGATACAGATCTAAGAACAGAAGGATAATTATG
  TGCTAGCTTATATATAGTTCCTTGTAAAAATAATGATACATGAAAAATTAGTAGAAAATG
  AAAATCTGCAGTTATCTGATATCTGAAAATGTTAAGCAATTTCCCCTGCATCTTAGTTCT
  AATCACTTTTGCAGAATAATCAAGCATATTTGGGTGAAGAGAGTATTTAATGATTCCATT
  TTTATTAATAGTGTAGTGTGCCCTTCAGAGCTTGGCGTAGTCTTTAAGAATCATCTGAAG
  TTTCTGGCTCAGCATGATGTTCCCTCTGGCCTTCAGCCTGCCACTAAAGAATGCCTTCTG
  AGGGTCAAGCTTGCCCAGGACCACCTCCATGAAATCTTCATCTGAAAGTATGATTGTTGT
  ATCAGCAGCACCTTTTGCAGGGCCTTGGTACACTTTTCCAGAACCACTTTTCAAGTCAAT
  AGTCCACTTAGCCCCAATATTTCCGCCTTTGGTTATATGCCACTCAAATACAGCATTTAC
  TTTTTTCACCACCTCATGCCCAATATCCTTTAAGCGGCGCCCCTATTCCTCAAATACAAA
  AGTACTCTGAAACTTCCCCGCCTCAGAAGGTGTCTTAACTGGAGACCAGAAGTTGGGGCA
  AGATCCCCATATGCATTTTGAATGACAATGTTTCCCACTCTCTTGAACCTTGGCTGGAAA
  AAGAATTCTGTTTCCTTCCTTACACTTCTAATTTGAAAAAGCTGCCCTGGATATATGGTT
  TTGACAACCAGCCTTATTGGCACTGATCTGGACACCTATTTTCGGAACTGCTGTACACAC
  GCTGGTAAAATCACATGGCATAACCCGTCAATGGGCTTGACAAACGGGCACTAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000414
• Insert Size: 2590 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000414.1, NP_000405.1
• RefSeq Size: 2593 bp
• RefSeq ORF: 2211 bp
• Locus ID: 3295
• UniProt ID: P51659
• Cytogenetics: 5q23.1
• Domains: adh_short, MaoC_dehydratas, SCP2
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Primary bile acid biosynthesis
• Gene Summary: The protein encoded by this gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathway for fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidation activity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present on chromosome 8. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1.