PON1 (NM_000446) Human Untagged Clone

CAT#: SC119866

PON1 (untagged)-Human paraoxonase 1 (PON1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PON1
• Synonyms: ESA; MVCD5; PON
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119866 sequence for NM_000446 edited (data generated by NextGen Sequencing)
  ATGGCGAAGCTGATTGCGCTCACCCTCTTGGGGATGGGACTGGCACTCTTCAGGAACCAC
  CAGTCTTCTTACCAAACACGACTTAATGCTCTCCGAGAGGTACAACCCGTAGAACTTCCT
  AACTGTAATTTAGTTAAAGGAATCGAAACTGGCTCTGAAGACATGGAGATACTGCCTAAT
  GGACTGGCTTTCATTAGCTCTGGATTAAAGTATCCTGGAATAAAGAGCTTCAACCCCAAC
  AGTCCTGGAAAAATACTTCTGATGGACCTGAATGAAGAAGATCCAACAGTGTTGGAATTG
  GGGATCACTGGAAGTAAATTTGATGTATCTTCATTTAACCCTCATGGGATTAGCACATTC
  ACAGATGAAGATAATGCCATGTACCTCCTGGTGGTGAACCATCCAGATGCCAAGTCCACA
  GTGGAGTTGTTTAAATTTCAAGAAGAAGAAAAATCGCTTTTGCATCTAAAAACCATCAGA
  CATAAACTTCTGCCTAATTTGAATGATATTGTTGCTGTGGGACCTGAGCACTTTTATGGC
  ACAAATGATCACTATTTTCTTGACCCCTACTTACAATCCTGGGAGATGTATTTGGGTTTA
  GCGTGGTCGTATGTTGTCTACTATAGTCCAAGTGAAGTTCGAGTGGTGGCAGAAGGATTT
  GATTTTGCTAATGGAATCAACATTTCACCCGATGGCAAGTATGTCTATATAGCTGAGTTG
  CTGGCTCATAAGATTCATGTGTATGAAAAGCATGCTAATTGGACTTTAACTCCATTGAAG
  TCCCTTGACTTTAATACCCTCGTGGATAACATATCTGTGGATCCTGAGACAGGAGACCTT
  TGGGTTGGATGCCATCCCAATGGCATGAAAATCTTCTTCTATGACTCAGAGAATCCTCCT
  GCATCAGAGGTGCTTCGAATCCAGAACATTCTAACAGAAGAACCTAAAGTGACACAGGTT
  TATGCAGAAAATGGCACAGTGTTGCAAGGCAGTACAGTTGCCTCTGTGTACAAAGGGAAA
  CTGCTGATTGGCACAGTGTTTCACAAAGCTCTTTACTGTGAGCTCTAA
  
  Clone variation with respect to NM_000446.5
  163 t=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000446 unedited
  TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAGAAGTTACACCAGGGACAG
  CATTTCGTTGGAAGGAGCAAAATGGGACTTTTGGCTGAAAGTGCTGAGCTCCTGCGGTGG
  GGGCTGACCGCAAGCCGCGCCTTCTGTGCACCTGGTCGGCCCAGCTAGCTGCGGACCCGG
  CGGGGAGGGGTGGGGCGGGCCAATCGGCGCTGCCCCAGCAGGGCTGCGGCTGCAGGCAGG
  CAGAGCCTCCTAGCCCGTCGGTGTCTGCGCCCATCGATCCCTTTGTCTATCCCCGACCAT
  GGCGAAGCTGATTGCGCTCACCCTCTTGGGGATGGGACTGGCACTCTTCAGGAACCACCA
  GTCTTCTTACCAAACACGACTTAATGCTCTCCGAGAGGTACAACCCGTAGAACTTCCTAA
  CTGTAATTTAGTTAAAGGAATCGAAACTGGCTCTGAAGACATGGAGATACTGCCTAATGG
  ACTGGCTTTCATTAGCTCTGGATTAAAGTATCCTGGAATAAAGAGCTTCAACCCCAACAG
  TCCTGGAAAAATACTTCTGATGGACCTGAATGAAGAAGATCCAACAGTGTTGGAATTGGG
  GATCACTGGAAGTAAATTTGATGTATCTTCATTTAACCCTCATGGGATTAGCACATTCAC
  AGATGAAGATAATGCCATGTACCTNCTGGTGGTGAACCATCCAGATGCCAAGTCCACAGT
  GGGAGTGTTTAAATTTCAAGAAGAAGAAAAATCGCTTTTGCATCTAAAAACCCATCAGAC
  ATAACTNCTGCCTAAATTGATGATATTGNTGCTGTGGGACCTGAGCACTTTATGGCACAA
  TGATCACTATTTTCTTGACCCCTACTACATNNCTGGAGAGTATTTGGTTTACCGTGGCGT
  ATGTTGCTACATANTCCAGTGAAGTCGAGTGTGGCAAAAGAATTGATTTGCTATGGAATC
  ACATTCACCGAGGCAGTATGCATAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000446 unedited
  GCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGTGGTTCTCAATTTATTGGCAA
  AACATTTTCCCGGAAATGCCAGACTATCATCATAACAAGTGCCTTATAAAAACTTCGTTT
  CTGGCTGGGTGTGGTGGCTCGCACCTGTAATCCCAGCACTTTGGAAGGCCAAGGCGGGTG
  GATCACCTGAGGTCAAGTGTTCGAGACCAGGCTGGCCAACATGGTGAAACCCCGTCTCTA
  CTAAAAATACAAAAATTATCCGGGCATGGTGGTGTGTGCCTGTAGTCCCAGCTATCCGGG
  AGGCTGAGGCACAAGAATCTCTTGAACCTCGGAGGTAGAAGTTGCAGTGAGCCAAGATCG
  CACCACTGCACTCCAGCCTGGGCAACAGAGTGAGATTCGGTCTCAAAAACAAAACAAAAA
  CAAGAAAAAAATTTGTTTCTAAAGTCACTGCCTTAACACCCTGAAGAAGTAATTCATCAT
  GAAGGTTATACCTTCAAATCAGAAAAACAAATAAATGAAAATGTGTAACCTTTATATATC
  AGCTTCTATAACAAAATGATAAAGTCATGTTTTCTTCCTAGTCATGTCTCAGTAGATAGC
  CAAAGGGGTTGGTGATCTTTTCTATTTATTTGTTTGTTTACCTTTCTATTATTTTTTTTC
  TTTTCAGCCTNCACAACAAGAAGTTTAAATATTAAACCAAAACCCACCAGCACTACCACC
  ATCTCAACACAGTGTAGTCTTTGGGGACACTTTTAACTTCCCCATGTCATTATGGGATTC
  ATCTGTTCCATCATCGAATGAACAAACTCCTTTACCAAGTCAAAGAACTCAAAGTACATT
  TGTGCCAGATCATAAACTCTTATTTTAGGGGGACACCCCCCAGAATTTAGATTTCTTGGC
  CTTGCCCCGAAATGAAAACAGATCAAGGGAGTGGAAATCAGTTATTTACTGAAAAGCTTT
  CGAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000446
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000446.3, NP_000437.3
• RefSeq Size: 2395 bp
• RefSeq ORF: 1068 bp
• Locus ID: 5444
• UniProt ID: P27169
• Cytogenetics: 7q21.3
• Domains: Arylesterase
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Metabolic pathways
• Gene Summary: This gene encodes a member of the paraoxonase family of enzymes and exhibits lactonase and ester hydrolase activity. Following synthesis in the kidney and liver, the enzyme is secreted into the circulation, where it binds to high density lipoprotein (HDL) particles and hydrolyzes thiolactones and xenobiotics, including paraoxon, a metabolite of the insecticide parathion. Polymorphisms in this gene may be associated with coronary artery disease and diabetic retinopathy. The gene is found in a cluster of three related paraoxonase genes on chromosome 7. [provided by RefSeq, Aug 2017]